FastQCFastQC Report
Fri 27 May 2016
SRR2139314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11192305
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158150.050.2734151
GTATCAA289450.038.656471
TCAACGC330000.033.639154
ATCAACG333500.033.2603233
TATCAAC340300.032.59572
CAACGCA342050.032.5441255
AACGCAG351700.031.698286
ACGCAGA410300.027.1174687
CGCAGAG416850.026.691378
GTGGTAT79150.025.6826881
TGGTATC74300.025.582582
GCAGAGT492150.022.607539
GAGTACT287700.021.17857612-13
CAGAGTA477850.019.84208310-11
CTTATAC68050.019.7853011
GTACATG344500.019.694481
GTACTTT318300.019.00477814-15
TACATGG340750.018.9686032
ACATGGG347450.018.4002083
AGAGTAC442000.018.1133410-11
Produced by FastQC (version 0.11.5)