##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139314_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11192305
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.89362396753841	31.0	18.0	33.0	18.0	33.0
2	27.912349154173334	29.0	25.0	32.0	18.0	33.0
3	28.189678622946747	29.0	27.0	31.0	18.0	33.0
4	31.21333773516715	33.0	31.0	33.0	27.0	33.0
5	31.857216989708554	33.0	32.0	33.0	30.0	34.0
6	35.910259950921635	38.0	36.0	38.0	31.0	38.0
7	36.24863421788452	38.0	37.0	38.0	33.0	38.0
8	36.60056047436163	38.0	37.0	38.0	34.0	38.0
9	36.846496409810136	38.0	38.0	38.0	35.0	38.0
10-11	36.892359840086556	38.0	38.0	38.0	35.0	38.0
12-13	36.95559721612304	38.0	38.0	38.0	35.0	38.0
14-15	36.9478310321243	38.0	38.0	38.0	35.0	38.0
16-17	36.96102331021179	38.0	38.0	38.0	35.0	38.0
18-19	36.97283361202183	38.0	38.0	38.0	35.0	38.0
20-21	36.98524924335241	38.0	38.0	38.0	35.5	38.0
22-23	36.996428091550825	38.0	38.0	38.0	36.0	38.0
24-25	36.98375072463104	38.0	38.0	38.0	36.0	38.0
26-27	36.955225548963774	38.0	38.0	38.0	35.5	38.0
28-29	36.93309235002006	38.0	38.0	38.0	35.0	38.0
30-31	36.9324066839526	38.0	38.0	38.0	35.0	38.0
32-33	36.90962481091497	38.0	38.0	38.0	35.0	38.0
34-35	36.88440308211004	38.0	38.0	38.0	35.0	38.0
36-37	36.85505262904161	38.0	38.0	38.0	35.0	38.0
38-39	36.83426716866582	38.0	38.0	38.0	35.0	38.0
40-41	36.83176352679324	38.0	38.0	38.0	35.0	38.0
42-43	36.776671938691564	38.0	38.0	38.0	35.0	38.0
44-45	36.73885190085488	38.0	38.0	38.0	35.0	38.0
46-47	36.6967351118798	38.0	38.0	38.0	35.0	38.0
48-49	36.65879663880638	38.0	38.0	38.0	34.0	38.0
50-51	36.62770820518873	38.0	38.0	38.0	34.0	38.0
52-53	36.605698564284666	38.0	38.0	38.0	34.0	38.0
54-55	36.591694810572434	38.0	38.0	38.0	34.0	38.0
56-57	36.57220108061212	38.0	38.0	38.0	34.0	38.0
58-59	36.55704821209914	38.0	38.0	38.0	34.0	38.0
60-61	36.55709774395728	38.0	38.0	38.0	34.0	38.0
62-63	36.5447561250929	38.0	38.0	38.0	34.0	38.0
64-65	36.535781123848224	38.0	38.0	38.0	34.0	38.0
66-67	36.50731794562185	38.0	38.0	38.0	34.0	38.0
68-69	36.49414805909999	38.0	38.0	38.0	34.0	38.0
70-71	36.495868087306135	38.0	38.0	38.0	34.0	38.0
72-73	36.480208467291526	38.0	38.0	38.0	34.0	38.0
74-75	36.47166984450553	38.0	38.0	38.0	34.0	38.0
76-77	36.45884576689578	38.0	38.0	38.0	34.0	38.0
78-79	36.4426725539519	38.0	38.0	38.0	34.0	38.0
80-81	36.43953041161447	38.0	38.0	38.0	34.0	38.0
82-83	36.417850784115984	38.0	38.0	38.0	34.0	38.0
84-85	36.409927076057514	38.0	38.0	38.0	34.0	38.0
86-87	36.39496197409461	38.0	38.0	38.0	34.0	38.0
88-89	36.39218508769214	38.0	38.0	38.0	34.0	38.0
90-91	36.39515557844863	38.0	38.0	38.0	34.0	38.0
92-93	36.36458154391478	38.0	38.0	38.0	34.0	38.0
94-95	36.35645649690376	38.0	37.5	38.0	34.0	38.0
96-97	36.351610463548084	38.0	37.0	38.0	34.0	38.0
98-99	36.38060704521153	38.0	37.0	38.0	34.0	38.0
100-101	35.83038741406769	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	38.0
20	111.0
21	496.0
22	2038.0
23	5974.0
24	13879.0
25	26309.0
26	44510.0
27	68015.0
28	97483.0
29	131163.0
30	170730.0
31	215021.0
32	276641.0
33	364471.0
34	514637.0
35	835862.0
36	2036825.0
37	6388093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.13593937977923	18.8968402844633	13.769978570097937	22.19724176565953
2	14.567169139868867	20.2980708620789	41.00597687428997	24.12878312376226
3	19.80932753297236	25.647995023666976	29.480225339538503	25.062452103822157
4	12.943491086063148	18.290423643744518	39.28021975812847	29.485865512063867
5	13.826091495901895	38.057806456780135	34.520981442594184	13.595120604723787
6	29.725038765473244	37.83214449570486	18.822548170372414	13.620268568449484
7	26.47257200371148	33.16455368219504	22.651938094967928	17.71093621912555
8	24.067812662360435	37.06459929388986	20.99202085718715	17.875567186562556
9	25.287355911047815	17.810111500714108	21.430107560506972	35.472425027731106
10-11	24.145290000585224	27.089093801500226	28.51229036378119	20.253325834133364
12-13	25.020364437888354	24.4490522729679	28.86456364439675	21.666019644746996
14-15	22.228689961577608	25.884010540638137	26.62982676083199	25.25747273695227
16-17	21.596838184806437	29.156822477586164	27.2806807891672	21.9656585484402
18-19	21.56849281716322	28.099448683716176	29.228505656341568	21.103552842779035
20-21	22.402607294746304	27.201784610034707	29.219710230494854	21.175897864724135
22-23	22.09765245983434	27.135503660026057	29.106470780238762	21.660373099900845
24-25	21.850873865079894	27.257741887935328	29.25746478037528	21.6339194666095
26-27	21.790546586743588	27.422462897288547	29.171407669850204	21.61558284611766
28-29	21.688348991714225	27.434655329076314	29.25867224463334	21.618323434576123
30-31	22.043426944544766	27.315097407840238	29.08808712621218	21.553388521402812
32-33	21.928263049402307	27.394825859842115	29.0383515458021	21.638559544953477
34-35	21.95147972964421	27.443893040105877	29.035684447504135	21.568942782745783
36-37	21.893932110155173	27.397023004449363	29.04276172224407	21.666283163151398
38-39	21.743143877713607	27.49005298670304	29.103072641165095	21.66373049441826
40-41	22.032569809592182	27.329892400185518	28.95931836845147	21.678219421770827
42-43	21.96971553421978	27.34233489915438	28.867318558951794	21.820631007674045
44-45	21.97183892502602	27.506512120062066	28.721185234310553	21.80046372060136
46-47	22.089068493598923	27.44132243688701	28.414033902622833	22.055575166891234
48-49	21.961765385991498	27.57189868929968	28.475286708425823	21.991049216283002
50-51	21.944852807975042	27.722132883110394	28.392889291161943	21.940125017752617
52-53	22.137351868285588	27.65100702100115	28.29635884089818	21.91528226981508
54-55	22.075420469458038	27.561995684210867	28.307787108184606	22.05479673814649
56-57	22.053032570731474	27.75929398097332	28.204598773301083	21.98307467499412
58-59	22.00363195690833	27.864797172135486	28.30645574374881	21.825115127207376
60-61	22.11399649986591	27.854340833237163	28.225785800069126	21.805876866827802
62-63	22.013660341330905	27.998327182326253	28.146739519749715	21.841272956593127
64-65	22.024561832116547	27.894392610594938	28.152277048768536	21.92876850851998
66-67	22.094533879908447	27.889794517767996	28.04371616959571	21.971955432727846
68-69	22.028360299308595	27.847220246326724	28.156434810600377	21.9679846437643
70-71	22.049636713864352	27.540174925761878	28.219819989791134	22.190368370582636
72-73	22.107417388174415	27.58434608244993	28.170932223610336	22.13730430576532
74-75	21.931550934992273	27.751265310157848	28.157837698067144	22.159346056782734
76-77	22.114495879275857	27.650724762553303	28.099445300983223	22.135334057187624
78-79	22.077363666018364	27.73802618088907	27.9983134476401	22.186296705452463
80-81	22.111531930047665	27.721576324144685	27.98313416657766	22.183757579229983
82-83	22.130541040705587	27.717983048887618	28.085813502185207	22.065662408221588
84-85	22.276570937203545	27.65614437734992	28.02366142504507	22.043623260401464
86-87	22.11353078083591	27.674972255828827	28.088777944698613	22.12271901863665
88-89	22.161944189221455	27.726139069797473	28.003270834866278	22.10864590611479
90-91	22.19755234026304	27.75722144695523	28.005145733177145	22.040080479604583
92-93	22.211491149259132	27.716121903726453	27.99106885346767	22.081318093546745
94-95	22.100535033712838	27.820622652458805	27.94414668883236	22.134695624996002
96-97	22.156648522821516	27.82536188956183	27.99717318427967	22.020816403336983
98-99	22.381202640692216	28.408088772108663	28.031410542063988	21.179298045135127
100-101	23.001694305382728	30.9076900756909	25.43110836715872	20.659507251767646
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2029.0
1	1896.5
2	2484.5
3	3482.0
4	4390.5
5	6298.0
6	8271.5
7	10419.5
8	13786.0
9	16152.0
10	15951.0
11	15112.5
12	15883.5
13	19434.5
14	26496.0
15	34842.5
16	41965.5
17	47142.5
18	48501.0
19	45879.5
20	41612.0
21	37807.0
22	37032.5
23	41026.5
24	49670.0
25	63947.5
26	83669.0
27	105862.0
28	129456.0
29	154134.5
30	178166.0
31	203265.0
32	230394.0
33	257863.5
34	284812.5
35	308780.5
36	334513.0
37	359638.5
38	377263.0
39	391322.0
40	409582.5
41	427571.0
42	438214.5
43	447077.5
44	462366.5
45	472139.5
46	469094.0
47	463900.5
48	457008.0
49	446086.5
50	432371.0
51	416347.0
52	396294.0
53	374230.0
54	350682.5
55	325363.0
56	299486.0
57	271934.5
58	243324.5
59	211610.5
60	175877.5
61	141808.5
62	110361.5
63	83784.5
64	62409.0
65	45437.0
66	33610.0
67	25760.0
68	19723.5
69	14249.0
70	10059.5
71	7154.0
72	4793.5
73	3055.5
74	1977.5
75	1203.5
76	709.5
77	429.0
78	277.0
79	184.5
80	128.0
81	85.0
82	61.5
83	43.5
84	28.0
85	19.0
86	12.0
87	6.5
88	3.5
89	2.0
90	2.5
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0011436428867869488
4	0.0
5	0.04629073278471236
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.127148518558063E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.6811619816414584E-5
28-29	2.2349528114298348E-5
30-31	4.473112323115805E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	3.616067144581167E-5
40-41	0.0
42-43	0.0
44-45	9.1804038404567E-6
46-47	2.9578674884608505E-4
48-49	0.0
50-51	3.7447829322530383E-5
52-53	9.414115798237234E-6
54-55	0.001893352382982776
56-57	0.0025475815617760672
58-59	8.624852964225403E-4
60-61	0.0028114866832944646
62-63	0.0048093378997616535
64-65	0.0022417860519490696
66-67	0.00771344436134673
68-69	0.00456018638076545
70-71	0.0018486739461784064
72-73	7.703273528742727E-4
74-75	7.554411791696576E-4
76-77	0.0
78-79	0.0
80-81	2.0758927714196066E-4
82-83	0.0
84-85	7.96386955877842E-4
86-87	8.454383443616069E-4
88-89	9.117226672650429E-5
90-91	4.545871250319158E-4
92-93	2.1849317492869476E-4
94-95	3.3085307487679306E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	94.0
20-21	410.0
22-23	977.0
24-25	1175.0
26-27	2572.0
28-29	5555.0
30-31	15353.0
32-33	20170.0
34-35	27739.0
36-37	43710.0
38-39	51491.0
40-41	52039.0
42-43	60754.0
44-45	72534.0
46-47	74004.0
48-49	66924.0
50-51	60236.0
52-53	58102.0
54-55	61909.0
56-57	65322.0
58-59	65934.0
60-61	66431.0
62-63	64428.0
64-65	72201.0
66-67	76197.0
68-69	76535.0
70-71	81348.0
72-73	69143.0
74-75	70835.0
76-77	75341.0
78-79	78562.0
80-81	78709.0
82-83	76448.0
84-85	76026.0
86-87	79559.0
88-89	82944.0
90-91	85911.0
92-93	85176.0
94-95	92735.0
96-97	159687.0
98-99	966180.0
100-101	7870905.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.89526170732758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.50637352729626	73.36758996311713
2	7.760494789537793	12.86616493114175
3	2.065197661618177	5.135853019115992
4	0.7402313062320237	2.454466714162422
5	0.35538567384422853	1.4729894220176143
6	0.1887766192830483	0.9389212355815699
7	0.11291200147381542	0.6551908938449061
8	0.0714457136448403	0.4738008904364657
9	0.0434754747862047	0.32435197742274846
>10	0.15099105610955374	1.919217913546059
>50	0.003638495847065449	0.1979444366266177
>100	9.82045996004591E-4	0.12704442266723406
>500	7.650845639069366E-5	0.04192150130168353
>1k	1.91258746078788E-5	0.024542679017905453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7869420106046072E-5	0.0	0.0	0.0
3	0.0	1.7869420106046072E-5	0.0	0.0	0.0
4	0.0	5.360826031813822E-5	0.0	0.0	0.0
5	0.0	5.360826031813822E-5	0.0	0.0	0.0
6	4.467355026511518E-5	5.360826031813822E-5	0.0	0.0	0.0
7	4.467355026511518E-5	5.360826031813822E-5	0.0	8.934710053023036E-6	0.0
8	4.467355026511518E-5	6.254297037116126E-5	0.0	8.934710053023036E-6	8.934710053023036E-6
9	4.467355026511518E-5	1.3402065079534556E-4	0.0	8.934710053023036E-6	1.7869420106046072E-5
10-11	4.9140905291626704E-5	1.3402065079534556E-4	0.0	1.7869420106046072E-5	1.7869420106046072E-5
12-13	5.8075615344649735E-5	1.4295536084836858E-4	0.0	2.680413015906911E-5	2.233677513255759E-5
14-15	6.254297037116126E-5	1.8762891111348377E-4	0.0	3.5738840212092144E-5	3.5738840212092144E-5
16-17	1.1615123068929948E-4	1.9656362116650682E-4	0.0	4.467355026511518E-5	3.5738840212092144E-5
18-19	1.1615123068929948E-4	2.0549833121952986E-4	0.0	4.467355026511518E-5	4.020619523860366E-5
20-21	1.1615123068929948E-4	2.1443304127255288E-4	0.0	5.360826031813822E-5	4.467355026511518E-5
22-23	1.2061858571581099E-4	2.4123717143162198E-4	0.0	6.254297037116126E-5	5.8075615344649735E-5
24-25	1.2955329576883404E-4	2.5463923651115655E-4	0.0	8.487974550371884E-5	7.147768042418429E-5
26-27	1.3402065079534556E-4	2.591065915376681E-4	0.0	1.5635742592790314E-4	7.147768042418429E-5
28-29	1.3402065079534556E-4	2.591065915376681E-4	0.0	3.0824749682929476E-4	7.147768042418429E-5
30-31	1.3402065079534556E-4	3.0378014180278324E-4	0.0	8.979383603288152E-4	7.147768042418429E-5
32-33	1.3402065079534556E-4	3.0378014180278324E-4	0.0	0.002385567584157151	7.147768042418429E-5
34-35	1.429553608483686E-4	3.305842719618523E-4	0.0	0.0041501728196292	7.147768042418429E-5
36-37	1.5189007090139162E-4	3.5738840212092146E-4	0.0	0.007045018876808665	8.041239047720733E-5
38-39	1.697594910074377E-4	3.7525782222696754E-4	0.0	0.011333679702259722	8.041239047720733E-5
40-41	1.697594910074377E-4	4.199313724920827E-4	0.0	0.016002065704964256	8.041239047720733E-5
42-43	1.697594910074377E-4	4.333334375716173E-4	0.0	0.020397943051051592	8.041239047720733E-5
44-45	1.742268460339492E-4	4.91409052916267E-4	0.0	0.0252092844146045	8.041239047720733E-5
46-47	1.8316155608697225E-4	5.360826031813822E-4	0.0	0.030820282327902966	1.0274916560976493E-4
48-49	1.920962661399953E-4	5.360826031813822E-4	0.0	0.03654743147189073	1.0721652063627644E-4
50-51	2.0103097619301834E-4	5.941582185260319E-4	0.0	0.04254262191746919	1.0721652063627644E-4
52-53	2.0549833121952983E-4	6.03092928579055E-4	0.0	0.047849839688964876	1.0721652063627644E-4
54-55	2.0996568624604136E-4	6.164949936585895E-4	0.0	0.05319726365569916	1.0721652063627644E-4
56-57	2.1443304127255288E-4	6.254297037116126E-4	0.0	0.05795052940390742	1.1168387566278796E-4
58-59	2.1443304127255288E-4	6.432991238176587E-4	0.0	0.06251616624100219	1.1615123068929948E-4
60-61	2.4123717143162198E-4	6.656358989502163E-4	0.0	0.06710413985322952	1.1615123068929948E-4
62-63	2.5017188148464503E-4	6.790379640297508E-4	0.0	0.07122750854269966	1.1615123068929948E-4
64-65	2.5017188148464503E-4	7.594503545069581E-4	0.0	0.07519898716126838	1.1615123068929948E-4
66-67	2.591065915376681E-4	7.683850645599812E-4	0.0	0.0793938335311627	1.1615123068929948E-4
68-69	2.591065915376681E-4	7.862544846660273E-4	0.0	0.08359314725608352	1.1615123068929948E-4
70-71	2.680413015906911E-4	7.907218396925388E-4	0.0	0.08755122380957274	1.2508594074232251E-4
72-73	3.0378014180278324E-4	8.085912597985849E-4	0.0	0.09106256486041078	1.3402065079534556E-4
74-75	3.0378014180278324E-4	8.309280349311425E-4	0.0	0.09394400885251072	1.3402065079534556E-4
76-77	3.0378014180278324E-4	9.068730703818383E-4	0.0	0.0962625661112702	1.3402065079534556E-4
78-79	3.2164956190882933E-4	9.292098455143959E-4	0.0	0.09844710271923433	1.3402065079534556E-4
80-81	3.2164956190882933E-4	9.292098455143959E-4	0.0	0.09941651875998733	1.3848800582185708E-4
82-83	3.2164956190882933E-4	9.336772005409074E-4	0.0	0.09993026458803615	1.4295536084836858E-4
84-85	3.2164956190882933E-4	9.426119105939304E-4	0.0	0.10032339183036917	1.4295536084836858E-4
86-87	3.2611691693534085E-4	9.649486857264879E-4	0.0	0.10044847777111149	1.4295536084836858E-4
88-89	3.395189820148754E-4	0.0010542957862567184	0.0	0.10048868396635009	1.5189007090139162E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15815	0.0	50.273415	1
GTATCAA	28945	0.0	38.65647	1
TCAACGC	33000	0.0	33.63915	4
ATCAACG	33350	0.0	33.260323	3
TATCAAC	34030	0.0	32.5957	2
CAACGCA	34205	0.0	32.544125	5
AACGCAG	35170	0.0	31.69828	6
ACGCAGA	41030	0.0	27.117468	7
CGCAGAG	41685	0.0	26.69137	8
GTGGTAT	7915	0.0	25.682688	1
TGGTATC	7430	0.0	25.58258	2
GCAGAGT	49215	0.0	22.60753	9
GAGTACT	28770	0.0	21.178576	12-13
CAGAGTA	47785	0.0	19.842083	10-11
CTTATAC	6805	0.0	19.785301	1
GTACATG	34450	0.0	19.69448	1
GTACTTT	31830	0.0	19.004778	14-15
TACATGG	34075	0.0	18.968603	2
ACATGGG	34745	0.0	18.400208	3
AGAGTAC	44200	0.0	18.11334	10-11
>>END_MODULE