FastQCFastQC Report
Fri 27 May 2016
SRR2139313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13390624
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136200.044.4283831
GTATCAA235450.034.3262861
ATCAACG295650.026.6150253
TCAACGC296500.026.510544
CAACGCA300950.026.118545
AACGCAG306600.025.7598956
TATCAAC317700.025.0580232
ACGCAGA355750.022.0717287
CGCAGAG363500.021.5896538
GTACATG341650.019.4239751
TACATGG340600.019.0176162
ACATGGG344550.018.4836253
AGAGTAC389950.018.14272310-11
GCAGAGT435600.017.8914459
GAGTACT244850.016.69995112-13
CATGGGG260350.016.5323314
CAGAGTA423650.016.24085810-11
AGTACTT276450.015.47126212-13
GTACTAG33550.014.822571
GTACTTT280850.014.53082814-15
Produced by FastQC (version 0.11.5)