##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139313_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13390624 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.882700387972957 31.0 31.0 34.0 30.0 34.0 2 32.021468678382725 33.0 31.0 34.0 30.0 34.0 3 32.087589271418565 33.0 31.0 34.0 30.0 34.0 4 35.78525429434804 37.0 35.0 37.0 35.0 37.0 5 35.650906559694306 37.0 35.0 37.0 33.0 37.0 6 35.64869381740537 37.0 35.0 37.0 33.0 37.0 7 35.52928280265356 37.0 35.0 37.0 33.0 37.0 8 35.52033176347869 37.0 35.0 37.0 33.0 37.0 9 37.132085629467305 39.0 37.0 39.0 33.0 39.0 10-11 37.00238652059829 39.0 37.0 39.0 33.0 39.0 12-13 36.963573131468706 39.0 37.0 39.0 33.0 39.0 14-15 38.12980156861995 40.0 37.0 41.0 33.0 41.0 16-17 38.12513714820161 40.0 37.0 41.0 33.0 41.0 18-19 38.145777261761665 40.0 37.0 41.0 33.0 41.0 20-21 38.13025852224809 40.0 37.0 41.0 33.0 41.0 22-23 38.04131852059791 40.0 37.0 41.0 33.0 41.0 24-25 37.94798061954563 40.0 37.0 41.0 33.0 41.0 26-27 37.78660457490365 40.0 37.0 41.0 32.0 41.0 28-29 37.650970335375916 39.5 37.0 41.0 32.0 41.0 30-31 37.51629737704618 39.0 36.5 41.0 32.0 41.0 32-33 37.67187153663485 39.5 37.0 41.0 32.0 41.0 34-35 37.635844904770835 40.0 37.0 41.0 32.0 41.0 36-37 37.50713571374595 39.5 36.5 41.0 32.0 41.0 38-39 37.343244920347104 39.0 36.0 41.0 31.0 41.0 40-41 37.28941388995878 39.0 36.0 41.0 31.0 41.0 42-43 37.17887543103927 39.0 36.0 40.0 31.0 41.0 44-45 37.24178440706004 39.0 36.0 40.5 31.0 41.0 46-47 37.39472759170232 39.0 36.0 41.0 31.0 41.0 48-49 37.3230314206658 39.0 36.0 41.0 31.0 41.0 50-51 37.180315644555236 39.0 36.0 41.0 31.0 41.0 52-53 37.039700673466285 39.0 35.5 41.0 31.0 41.0 54-55 36.907423138984754 39.0 35.0 40.0 31.0 41.0 56-57 36.752890603952835 39.0 35.0 40.0 30.5 41.0 58-59 36.60959676875347 38.0 35.0 40.0 30.0 41.0 60-61 36.370099670972124 38.0 35.0 40.0 30.0 41.0 62-63 36.10395944897323 38.0 34.0 40.0 29.0 41.0 64-65 35.87825233389053 37.5 34.0 40.0 29.0 41.0 66-67 35.616200884061044 37.0 34.0 40.0 29.0 41.0 68-69 35.32456361158265 36.5 34.0 39.0 29.0 41.0 70-71 35.01572483368312 36.0 33.5 39.0 29.0 40.0 72-73 34.68360646018975 35.5 33.0 38.5 28.0 40.0 74-75 34.345928842645904 35.0 33.0 38.0 28.0 40.0 76-77 33.228615165699054 34.5 31.5 36.5 26.5 39.0 78-79 33.61156775364476 35.0 32.0 36.5 27.5 39.0 80-81 33.49494549181743 35.0 32.0 36.0 28.0 39.0 82-83 33.26130710040563 35.0 32.0 36.0 27.0 37.5 84-85 33.05175461374153 34.5 32.0 35.5 27.0 37.0 86-87 32.83855286863623 34.0 32.0 35.0 27.0 36.5 88-89 32.61527355322011 34.0 32.0 35.0 27.0 36.0 90-91 32.48421062289259 34.0 31.5 35.0 27.0 36.0 92-93 32.412073173771944 34.0 31.0 35.0 27.0 35.5 94-95 32.3791821638387 34.0 31.0 35.0 27.0 35.0 96-97 32.28607154730934 34.0 31.0 35.0 27.0 35.0 98-99 32.442932104002125 34.0 31.0 35.0 27.0 35.0 100-101 31.69966379500685 33.0 30.0 34.5 26.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 2.0 18 3.0 19 19.0 20 85.0 21 627.0 22 3047.0 23 9955.0 24 26042.0 25 55533.0 26 99472.0 27 156714.0 28 224367.0 29 298897.0 30 378675.0 31 466512.0 32 577010.0 33 719926.0 34 933767.0 35 1241856.0 36 1782799.0 37 2598361.0 38 2609138.0 39 1165305.0 40 42512.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.17671230257828 22.224953818432958 15.086630764929252 24.51170311405951 2 14.00736867921496 22.630473896258962 40.89556472246693 22.46659270205915 3 16.927739887252454 27.58444266674951 31.53751460723563 23.950302838762404 4 12.069079081004737 19.46487333226592 39.50160948436757 28.964438102361772 5 12.577912724604918 38.4732854869198 36.35790236511756 12.590899423357715 6 28.95896412295648 39.61580132486731 18.90820024518648 12.517034306989727 7 25.372469572739853 32.97959826218703 24.3496120867855 17.298320078287613 8 23.830263623263562 37.657438518175105 21.482441744313036 17.029856114248297 9 25.231632222665652 16.340433425656638 21.446483748628893 36.98145060304882 10-11 23.774007843099767 27.046614855289793 29.71147946503464 19.4678978365758 12-13 24.613998570940385 24.55842237075733 29.621935467682466 21.205643590619825 14-15 21.81563383453975 25.889122120074465 26.93686268840048 25.358381356985305 16-17 21.57306485493133 28.945693643552385 27.577669270677752 21.903572230838535 18-19 21.601324420669574 28.308703946597802 29.005640109212354 21.08433152352027 20-21 22.38182538202199 27.53430650147342 28.73322156892509 21.350646547579505 22-23 22.133394958787854 27.463547380954285 28.6188005787395 21.78425708151836 24-25 21.899996877199687 27.657809369028485 28.6457871854918 21.79640656828003 26-27 21.94418962378755 27.751432504388628 28.51922399047766 21.785153881346158 28-29 21.896357150099796 27.81808572337001 28.507003133740454 21.778553992789735 30-31 22.17222073790258 27.691229757034634 28.414941010958174 21.721608494104615 32-33 22.013615116682672 27.85083247009271 28.384364697667696 21.751187715556924 34-35 22.066370001565772 27.841901937956358 28.450389386585694 21.641338673892175 36-37 22.06340134448096 27.728288855449335 28.453957326508306 21.754352473561404 38-39 21.900225964348895 27.804401946598784 28.481621279549103 21.813750809503215 40-41 22.085377130783087 27.662688334584306 28.3942284234819 21.85770611115071 42-43 22.02175057340925 27.80033745907793 28.20419820478693 21.97371376272589 44-45 22.044030244334838 27.92588056395698 28.030711323460565 21.99937786824762 46-47 22.14893028589487 27.794437721389187 27.849384027139244 22.207247965576695 48-49 22.103272218882932 27.895979723500396 27.859352753492654 22.141395304124018 50-51 22.073495106421028 27.996877593215096 27.744485054039338 22.185142246324542 52-53 22.15692839676324 27.91139489997635 27.67495419698977 22.25672250627064 54-55 22.122581794595444 27.832173982945918 27.71205455079886 22.333189671659778 56-57 22.190481115186284 27.888739258207202 27.69804618381324 22.222733442793274 58-59 22.15647661717219 27.92150981805363 27.727217645623366 22.194795919150824 60-61 22.204735932432246 27.917631290259052 27.663832762446646 22.213800014862056 62-63 22.169617245873404 28.006409396236688 27.58995997264359 22.234013385246318 64-65 22.081466361706987 27.95000796353951 27.607118238922585 22.36140743583092 66-67 22.210799914015926 28.018049664539785 27.415300446173447 22.355849975270843 68-69 22.137031215318803 27.991225179741964 27.48358151286204 22.38816209207719 70-71 22.143155259139053 27.780024686488947 27.533756414082028 22.543063640289972 72-73 22.157977509818377 27.80392404029468 27.51591649372753 22.522181956159407 74-75 22.086820749935985 27.860915241420088 27.51882103457665 22.53344297406728 76-77 22.253246416790404 27.846553301637073 27.42732486902645 22.472875412546074 78-79 22.26871896251352 27.82351226347576 27.362372721453166 22.545396052557557 80-81 22.278863219941634 27.792872611960778 27.383037473674037 22.545226694423555 82-83 22.31122625538685 27.743749797873164 27.483085072243863 22.461938874496127 84-85 22.40553936212264 27.77775098712108 27.416976956328735 22.39973269442754 86-87 22.221634189037875 27.813804498544847 27.516303514895068 22.448257797522214 88-89 22.24021059999727 27.768409021784958 27.49068568357309 22.500694694644682 90-91 22.294019145982535 27.713874073597378 27.602686058683002 22.389420721737086 92-93 22.334166789233713 27.63959956271519 27.59767948330895 22.42855416474215 94-95 22.153188506584538 27.674813171286335 27.73388084909097 22.43811747303816 96-97 22.030777691049252 27.743333228298344 27.861346040317308 22.364543040335093 98-99 22.167871299122467 28.372198391505265 27.96392710099913 21.49600320837314 100-101 22.48012779291052 31.217312149731203 25.178965253612915 21.123594803745362 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1683.0 1 1692.0 2 2949.5 3 4447.0 4 6056.5 5 9632.5 6 12495.5 7 14337.0 8 15360.5 9 14981.5 10 13995.5 11 13179.0 12 14802.5 13 19649.0 14 30765.5 15 46710.0 16 59396.5 17 65558.0 18 66931.0 19 65554.5 20 62672.0 21 60655.5 22 61895.0 23 68952.5 24 82983.0 25 104429.0 26 133010.5 27 163281.5 28 193573.5 29 224237.5 30 251939.5 31 280695.0 32 311778.5 33 342407.5 34 372543.5 35 400441.0 36 429994.5 37 458654.0 38 479465.0 39 493421.0 40 509312.0 41 525670.0 42 536170.0 43 545863.0 44 555507.5 45 564536.5 46 569158.5 47 570129.0 48 570712.0 49 562438.5 50 547705.0 51 531388.0 52 508068.5 53 479585.5 54 448934.5 55 415520.5 56 381086.5 57 343842.5 58 303186.0 59 260537.5 60 216268.0 61 173986.0 62 137124.5 63 106231.0 64 79547.5 65 58503.5 66 43141.5 67 32305.0 68 24378.0 69 17761.5 70 12823.5 71 9237.5 72 6572.5 73 4540.0 74 3067.0 75 2092.0 76 1459.5 77 987.0 78 654.0 79 420.5 80 277.5 81 197.0 82 138.0 83 91.0 84 65.0 85 53.5 86 45.5 87 35.0 88 26.5 89 19.0 90 13.0 91 10.0 92 7.5 93 5.0 94 2.0 95 1.0 96 1.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.003256009578045056 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.0828472220562687E-4 20-21 0.0020276258987647834 22-23 4.033323919754481E-4 24-25 3.361865234551921E-5 26-27 3.325767523618555E-4 28-29 0.003424653949186809 30-31 3.817876796367178E-4 32-33 6.002538773774368E-5 34-35 0.0021523730345438187 36-37 0.0562477461877336 38-39 0.031274132669825025 40-41 0.04110665744277117 42-43 8.3440808442541E-4 44-45 0.002432335657331305 46-47 0.0014060322090077512 48-49 4.933972701920644E-4 50-51 0.0 52-53 9.895363827383975E-4 54-55 0.002360441262607804 56-57 0.001156428133814925 58-59 0.0037637710415456063 60-61 0.0014515824464016896 62-63 8.536066331381362E-5 64-65 7.510273536247688E-4 66-67 3.975873699337703E-4 68-69 1.8588810943250708E-4 70-71 0.001134905295293162 72-73 0.001791659144578253 74-75 7.143618580174007E-4 76-77 0.0011259783864477429 78-79 2.085334642417953E-4 80-81 0.002262529152327086 82-83 0.0026957672540112378 84-85 0.003937242932711076 86-87 0.004230627101761138 88-89 0.07004617004589211 90-91 7.302159800254935E-4 92-93 5.913318809863953E-5 94-95 4.415447708735875E-5 96-97 0.008371597844679526 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 344.0 20-21 1289.0 22-23 2495.0 24-25 4614.0 26-27 6353.0 28-29 10741.0 30-31 28601.0 32-33 36880.0 34-35 52052.0 36-37 82441.0 38-39 94461.0 40-41 100666.0 42-43 111095.0 44-45 129532.0 46-47 131511.0 48-49 125431.0 50-51 117184.0 52-53 104670.0 54-55 113858.0 56-57 121304.0 58-59 131739.0 60-61 136576.0 62-63 129413.0 64-65 136461.0 66-67 147132.0 68-69 149125.0 70-71 152726.0 72-73 146540.0 74-75 147435.0 76-77 148057.0 78-79 161523.0 80-81 167828.0 82-83 162509.0 84-85 163316.0 86-87 173794.0 88-89 191342.0 90-91 213249.0 92-93 230516.0 94-95 298848.0 96-97 377348.0 98-99 1157459.0 100-101 7292166.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.67690456722174 #Duplication Level Percentage of deduplicated Percentage of total 1 89.22827902158495 74.66346188386598 2 7.238263148969014 12.113509094974361 3 1.9061317106279678 4.784976037283148 4 0.733822326052454 2.456159229855515 5 0.3462050336326782 1.4484682779986702 6 0.17384927491940494 0.8728301511907047 7 0.10729867221264627 0.6284894528449048 8 0.06606398731460833 0.4422423969483703 9 0.047012190995760955 0.35404511575034614 >10 0.14875383560352473 1.8994858625517752 >50 0.0034308230105741595 0.1919741649457586 >100 8.264078146796494E-4 0.10557208408075512 >500 6.356726179268818E-5 0.03878624770971142 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.467911876250128E-6 2 0.0 2.2403735628750384E-5 0.0 0.0 7.467911876250128E-6 3 0.0 2.2403735628750384E-5 0.0 0.0 7.467911876250128E-6 4 0.0 5.22753831337509E-5 0.0 0.0 7.467911876250128E-6 5 0.0 5.22753831337509E-5 0.0 0.0 7.467911876250128E-6 6 5.974329501000103E-5 5.22753831337509E-5 0.0 7.467911876250128E-6 7.467911876250128E-6 7 5.974329501000103E-5 5.22753831337509E-5 0.0 7.467911876250128E-6 1.4935823752500257E-5 8 5.974329501000103E-5 5.974329501000103E-5 0.0 7.467911876250128E-6 1.4935823752500257E-5 9 5.974329501000103E-5 8.214703063875141E-5 0.0 7.467911876250128E-6 1.4935823752500257E-5 10-11 5.974329501000103E-5 8.214703063875141E-5 0.0 7.467911876250128E-6 1.4935823752500257E-5 12-13 6.721120688625115E-5 8.961494251500154E-5 0.0 1.4935823752500257E-5 1.4935823752500257E-5 14-15 6.721120688625115E-5 1.568261494012527E-4 0.0 2.2403735628750384E-5 2.2403735628750384E-5 16-17 1.0828472220562687E-4 1.568261494012527E-4 0.0 2.987164750500051E-5 2.9871647505000514E-5 18-19 1.1201867814375193E-4 1.7549592909187802E-4 0.0 4.480747125750077E-5 2.9871647505000514E-5 20-21 1.1201867814375193E-4 2.0163362065875348E-4 0.0 7.841307470062635E-5 2.9871647505000514E-5 22-23 1.1575263408187698E-4 2.389731800400041E-4 0.0 2.576429597306294E-4 3.733955938125064E-5 24-25 1.3068845783437724E-4 2.987164750500051E-4 0.0 8.14002394511264E-4 4.480747125750077E-5 26-27 1.344224137725023E-4 3.2858812255500563E-4 0.0 0.002400933668214416 4.480747125750077E-5 28-29 1.344224137725023E-4 3.3232207849313074E-4 0.0 0.013726022028547735 4.480747125750077E-5 30-31 1.4189032564875241E-4 3.845974616268816E-4 0.0 0.022105019153700382 4.480747125750077E-5 32-33 1.4935823752500256E-4 3.8833141756500667E-4 0.0 0.03121587164272554 4.480747125750077E-5 34-35 1.568261494012527E-4 4.3687284476063247E-4 0.0 0.04252229022336823 6.721120688625115E-5 36-37 1.568261494012527E-4 4.8541427195625833E-4 0.0 0.058768732510150384 6.721120688625115E-5 38-39 1.6802801721562787E-4 5.22753831337509E-4 0.0 0.09057456919109969 6.721120688625115E-5 40-41 1.7176197315375296E-4 6.273045976050108E-4 0.0 0.15051949782175947 6.721120688625115E-5 42-43 1.7176197315375296E-4 6.45974377295636E-4 0.0 0.17860258043239807 6.721120688625115E-5 44-45 1.7176197315375296E-4 7.54259099501263E-4 0.0 0.21985159168086565 6.721120688625115E-5 46-47 1.7176197315375296E-4 8.364061301400144E-4 0.0 0.26547679928881585 6.721120688625115E-5 48-49 1.7549592909187802E-4 8.364061301400144E-4 0.0 0.304952181466674 7.467911876250128E-5 50-51 1.9043175284437828E-4 8.998833810881404E-4 0.0 0.34402429640321464 7.467911876250128E-5 52-53 2.0163362065875348E-4 9.110852489025156E-4 0.0 0.3919570887809261 7.467911876250128E-5 54-55 2.0163362065875348E-4 9.148192048406406E-4 0.0 0.4862506780863983 7.467911876250128E-5 56-57 2.0163362065875348E-4 9.26021072655016E-4 0.0 0.5796854575261019 7.467911876250128E-5 58-59 2.0163362065875348E-4 9.372229404693911E-4 0.0 0.6550665600049707 7.467911876250128E-5 60-61 2.0910153253500362E-4 9.521587642218913E-4 0.0 0.6978240894524408 7.467911876250128E-5 62-63 2.31505268163754E-4 9.670945879743916E-4 0.0 0.7236817343239568 7.467911876250128E-5 64-65 2.31505268163754E-4 0.0010977830458087688 0.0 0.7425232759877358 7.467911876250128E-5 66-67 2.427071359781292E-4 0.0011239207373756443 0.0 0.7529820865704242 7.467911876250128E-5 68-69 2.4644109191625425E-4 0.001168728208633145 0.0 0.7636163930822044 7.467911876250128E-5 70-71 2.4644109191625425E-4 0.0012098017239525207 0.0 0.7733321464332058 7.467911876250128E-5 72-73 2.6511087160687954E-4 0.001217269635828771 0.0 0.7812556009339071 8.214703063875141E-5 74-75 2.688448275450046E-4 0.0012583431511481468 0.0 0.7887048430304666 8.214703063875141E-5 76-77 2.725787834831297E-4 0.0013106185342818975 0.0 0.795463303278473 8.214703063875141E-5 78-79 2.7631273942125477E-4 0.001336756225848773 0.0 0.8016243305763795 8.214703063875141E-5 80-81 2.800466953593798E-4 0.001336756225848773 0.0 0.8055599201351632 8.214703063875141E-5 82-83 2.837806512975049E-4 0.0013442241377250232 0.0 0.8089167465235376 8.961494251500154E-5 84-85 2.9498251911188006E-4 0.0013516920496012732 0.0 0.8117433511686983 8.961494251500154E-5 86-87 2.987164750500051E-4 0.001389031608982524 0.0 0.812183957969397 8.961494251500154E-5 88-89 2.987164750500051E-4 0.0014487749039925248 0.0 0.8122549031322215 8.961494251500154E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13620 0.0 44.428383 1 GTATCAA 23545 0.0 34.326286 1 ATCAACG 29565 0.0 26.615025 3 TCAACGC 29650 0.0 26.51054 4 CAACGCA 30095 0.0 26.11854 5 AACGCAG 30660 0.0 25.759895 6 TATCAAC 31770 0.0 25.058023 2 ACGCAGA 35575 0.0 22.071728 7 CGCAGAG 36350 0.0 21.589653 8 GTACATG 34165 0.0 19.423975 1 TACATGG 34060 0.0 19.017616 2 ACATGGG 34455 0.0 18.483625 3 AGAGTAC 38995 0.0 18.142723 10-11 GCAGAGT 43560 0.0 17.891445 9 GAGTACT 24485 0.0 16.699951 12-13 CATGGGG 26035 0.0 16.532331 4 CAGAGTA 42365 0.0 16.240858 10-11 AGTACTT 27645 0.0 15.471262 12-13 GTACTAG 3355 0.0 14.82257 1 GTACTTT 28085 0.0 14.530828 14-15 >>END_MODULE