FastQCFastQC Report
Fri 27 May 2016
SRR2139312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12740945
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153050.045.2742461
GTATCAA269850.034.7672231
ATCAACG337150.027.1595843
TCAACGC338750.027.0067164
CAACGCA344200.026.5911945
AACGCAG349950.026.2256726
TATCAAC358300.025.8824062
ACGCAGA412450.022.1910277
CGCAGAG416200.021.981088
GTACATG417650.020.0308171
TACATGG416150.019.6927532
ACATGGG423050.018.9576993
GCAGAGT493700.018.5136649
AGAGTAC446200.018.3334210-11
GAGTACT250350.017.04893712-13
CATGGGG280750.016.5822334
CAGAGTA484000.016.42840210-11
AGTACTT281050.016.00151812-13
GTACTTT289600.014.78963414-15
GTGGTAT75150.014.6841261
Produced by FastQC (version 0.11.5)