##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139312_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12740945
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85727149752236	31.0	31.0	34.0	30.0	34.0
2	31.996420987611202	33.0	31.0	34.0	30.0	34.0
3	32.058549895631764	33.0	31.0	34.0	30.0	34.0
4	35.76506271709045	37.0	35.0	37.0	35.0	37.0
5	35.63197651351607	37.0	35.0	37.0	33.0	37.0
6	35.634297455957935	37.0	35.0	37.0	33.0	37.0
7	35.512580110815954	37.0	35.0	37.0	33.0	37.0
8	35.503761534171915	37.0	35.0	37.0	33.0	37.0
9	37.10766783782522	39.0	37.0	39.0	33.0	39.0
10-11	36.97755009538146	39.0	37.0	39.0	33.0	39.0
12-13	36.93738941656211	39.0	37.0	39.0	33.0	39.0
14-15	38.087174459979224	40.0	37.0	41.0	33.0	41.0
16-17	38.08510141908626	40.0	37.0	41.0	33.0	41.0
18-19	38.11606019019783	40.0	37.0	41.0	33.0	41.0
20-21	38.09967628882737	40.0	37.0	41.0	33.0	41.0
22-23	38.01085797008302	40.0	37.0	41.0	33.0	41.0
24-25	37.911914306332015	40.0	37.0	41.0	33.0	41.0
26-27	37.74946959915579	40.0	37.0	41.0	32.0	41.0
28-29	37.61695575525569	39.5	37.0	41.0	32.0	41.0
30-31	37.47863061065145	39.0	36.0	41.0	32.0	41.0
32-33	37.62657284371474	39.5	37.0	41.0	32.0	41.0
34-35	37.58888438115145	40.0	36.5	41.0	32.0	41.0
36-37	37.458353887044126	39.5	36.0	41.0	31.5	41.0
38-39	37.29457392065767	39.0	36.0	41.0	31.0	41.0
40-41	37.23430253708174	39.0	36.0	40.5	31.0	41.0
42-43	37.120152046755685	39.0	36.0	40.0	31.0	41.0
44-45	37.185916686540594	39.0	36.0	40.5	31.0	41.0
46-47	37.33777514333788	39.0	36.0	41.0	31.0	41.0
48-49	37.265338662850795	39.0	36.0	41.0	31.0	41.0
50-51	37.12261727371018	39.0	36.0	41.0	31.0	41.0
52-53	36.97712146802296	39.0	35.0	40.0	31.0	41.0
54-55	36.84263033760388	39.0	35.0	40.0	31.0	41.0
56-57	36.68622322254066	38.5	35.0	40.0	30.0	41.0
58-59	36.54262904906813	38.0	35.0	40.0	30.0	41.0
60-61	36.297394061300174	38.0	34.5	40.0	30.0	41.0
62-63	36.02848067136294	38.0	34.0	40.0	29.0	41.0
64-65	35.80119299453165	37.0	34.0	40.0	29.0	41.0
66-67	35.53767155466756	37.0	34.0	39.5	29.0	41.0
68-69	35.24537301644254	36.0	34.0	39.0	29.0	41.0
70-71	34.9372535861438	36.0	33.5	39.0	28.5	40.0
72-73	34.60363319875273	35.5	33.0	38.5	28.0	40.0
74-75	34.27032099053574	35.0	33.0	37.5	28.0	40.0
76-77	33.15633507263806	34.0	31.5	36.0	26.5	39.0
78-79	33.54514820641198	35.0	32.0	36.5	27.5	39.0
80-81	33.432497457898975	35.0	32.0	36.0	27.5	38.5
82-83	33.19996060515349	35.0	32.0	36.0	27.0	37.5
84-85	32.99101274669445	34.5	32.0	35.0	27.0	37.0
86-87	32.77717490319712	34.0	32.0	35.0	27.0	36.5
88-89	32.551856950744046	34.0	32.0	35.0	26.5	36.0
90-91	32.42151817651551	34.0	31.0	35.0	26.0	36.0
92-93	32.350322557676016	34.0	31.0	35.0	26.5	35.5
94-95	32.31789079457503	34.0	31.0	35.0	27.0	35.0
96-97	32.2221281235427	34.0	31.0	35.0	26.5	35.0
98-99	32.387548579732375	34.0	31.0	35.0	27.0	35.0
100-101	31.604774695881858	33.0	30.0	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0
19	13.0
20	103.0
21	642.0
22	2898.0
23	9780.0
24	25987.0
25	54385.0
26	96917.0
27	152752.0
28	217131.0
29	287626.0
30	364111.0
31	449897.0
32	555152.0
33	696875.0
34	902906.0
35	1200055.0
36	1722496.0
37	2477922.0
38	2427924.0
39	1060161.0
40	35209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.24451796942848	22.267225861190045	15.267125005248824	24.221131164132643
2	14.091692381834827	22.767717368226688	40.806229879671456	22.334360370267024
3	16.970978212369648	27.854307510157213	31.491753555171933	23.68296072230121
4	12.00399185460733	19.57649138270356	39.46866578577963	28.950850976909482
5	12.505979736981834	38.67598518006317	36.1563055173694	12.661729565585597
6	28.69214960114811	39.65778833516666	19.01303239280917	12.637029670876062
7	25.352083381570207	33.08828348289707	24.23737799668706	17.322255138845666
8	23.99534728389456	37.51032596090793	21.427288164260972	17.06703859093654
9	25.44987832535185	16.228756972108428	21.382966491104074	36.938398211435654
10-11	23.843965263173178	27.11253757080028	29.516837251867894	19.526659914158646
12-13	24.749353364291267	24.66028618756301	29.479964005809617	21.110396442336107
14-15	21.92327963114196	25.94174137004751	26.911665500478964	25.223313498331564
16-17	21.5051081375832	29.024675956139834	27.52620390402753	21.94401200224944
18-19	21.555084641168563	28.261327947438076	29.104378419367784	21.079208992025585
20-21	22.539550660618332	27.39225742873071	28.766291756041284	21.301900154609672
22-23	22.19364352370368	27.374358730808535	28.68270586210463	21.749291883383158
24-25	21.8714829875087	27.580193332018148	28.773528294609026	21.774795385864127
26-27	21.88229947196567	27.6790360637952	28.650220045070217	21.78844441916891
28-29	21.781463968072323	27.713235303941598	28.67361280137617	21.83168792660991
30-31	22.049989637325268	27.579495563563484	28.61493292115539	21.75558187795586
32-33	21.891575159930163	27.77913940203063	28.52565564968268	21.80362978835653
34-35	21.994657386705242	27.817564701629454	28.565515530218725	21.622262381446575
36-37	21.98556892255329	27.733825910208417	28.492495605939773	21.788109561298523
38-39	21.88130636464282	27.79758375960991	28.55163469297139	21.769475182775878
40-41	22.035111852582638	27.663399461950462	28.482187157615797	21.819301527851103
42-43	21.993111082093804	27.73680980527635	28.286444276664437	21.983634835965407
44-45	22.018996087592296	27.921720654992782	28.103809929135064	21.955473328279858
46-47	22.17835275921668	27.78811280316036	27.848713759532817	22.18482067809014
48-49	22.09945163789431	27.904834210226802	27.834012876499386	22.161701275379496
50-51	22.085757511350117	27.991751777730546	27.72271081492721	22.199779895992126
52-53	22.206682665366237	27.913598466862588	27.672373154695446	22.20734571307573
54-55	22.16547040216588	27.824139621937828	27.70145416496882	22.308935810927473
56-57	22.24752503736204	27.87170215697114	27.644608967238245	22.23616383842858
58-59	22.16288656850665	27.96355090382593	27.685436045610007	22.188126482057413
60-61	22.26681764722342	27.94503426113244	27.614635822528278	22.17351226911586
62-63	22.160935628146696	27.991163263538105	27.632475096646168	22.215426011669027
64-65	22.16214220349201	27.920842312553205	27.638632507074163	22.27838297688062
66-67	22.2329738556754	27.98075465301506	27.470286597546785	22.315984893762757
68-69	22.169580128620215	27.94491299760928	27.52762475287408	22.35788212089642
70-71	22.190796269871147	27.696529327107072	27.61228939484009	22.500385008181688
72-73	22.20189472467024	27.791223976155845	27.537787600369484	22.469093698804432
74-75	22.11021190111865	27.807381783873357	27.590363239873327	22.492043075134667
76-77	22.24913934610378	27.75423180212509	27.522251427879795	22.474377423891333
78-79	22.17716419670962	27.780626315169716	27.47835175500529	22.56385773311538
80-81	22.206038051507353	27.771037854144236	27.476984590284076	22.545939504064332
82-83	22.246973645112124	27.693565809370668	27.56555889092609	22.49390165459112
84-85	22.288084251835567	27.712374669294874	27.52688661558883	22.472654463280726
86-87	22.14025791882804	27.76007824666724	27.60338976471828	22.496274069786438
88-89	22.179744498037326	27.659637116697482	27.593348180707704	22.567270204557484
90-91	22.186552185684537	27.599560568198527	27.72882151141447	22.48506573470247
92-93	22.147735023327566	27.564714226669455	27.749979731181607	22.53757101882137
94-95	21.93739692755472	27.588634297595966	27.853311353860317	22.620657420988998
96-97	21.877064886510972	27.666133187993484	27.931663638820176	22.52513828667537
98-99	22.00146358844812	28.305819743629367	28.044423613071146	21.64829305485136
100-101	25.63704627970719	35.63023580765923	28.931367461636174	9.801350450997406
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1927.0
1	1946.0
2	3263.0
3	4823.0
4	6522.5
5	10232.0
6	13180.5
7	15216.0
8	16521.5
9	16243.0
10	15107.5
11	14107.5
12	15673.0
13	20839.5
14	33121.0
15	50401.0
16	63767.0
17	69742.0
18	70232.5
19	67410.0
20	63113.5
21	59993.5
22	60575.0
23	67443.0
24	81434.0
25	102483.0
26	129751.5
27	157664.5
28	185440.5
29	213956.0
30	239236.0
31	264639.0
32	293958.5
33	324917.5
34	352989.0
35	379116.0
36	408546.5
37	435482.0
38	454882.5
39	467526.0
40	480777.0
41	495575.0
42	505836.0
43	515948.5
44	526672.0
45	535556.0
46	541468.5
47	544617.0
48	542094.0
49	531989.0
50	518533.5
51	502206.5
52	479821.0
53	454245.5
54	427591.5
55	395646.5
56	361453.0
57	325623.5
58	288119.5
59	250687.0
60	211436.0
61	171751.0
62	135173.5
63	104492.5
64	78490.5
65	57947.5
66	43134.5
67	32461.5
68	24524.5
69	17877.0
70	12806.5
71	9335.0
72	6650.5
73	4612.5
74	3230.5
75	2298.5
76	1585.0
77	1058.5
78	722.0
79	501.5
80	357.0
81	252.5
82	180.0
83	119.0
84	79.0
85	57.0
86	45.0
87	39.0
88	32.0
89	23.5
90	15.5
91	8.5
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0032179716653670505
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	9.02601808578563E-5
20-21	0.0020132799556434786
22-23	3.1792289121746256E-4
24-25	3.1407364390950545E-5
26-27	2.6314064486503223E-4
28-29	0.003367536422342665
30-31	3.4227396198044434E-4
32-33	5.915339192253335E-5
34-35	0.002088707238169669
36-37	0.05485836706132768
38-39	0.03090722921451822
40-41	0.04124605667740394
42-43	7.927298783509251E-4
44-45	0.002146381318061167
46-47	0.001352477893002072
48-49	4.86453976128026E-4
50-51	0.0
52-53	9.410911181531342E-4
54-55	0.0019294680577426337
56-57	0.0011465698618749521
58-59	0.0035199827304977426
60-61	0.0014658812572912556
62-63	1.3071544847005455E-4
64-65	7.703740927477529E-4
66-67	3.5987314748376693E-4
68-69	1.40209775322715E-4
70-71	0.0010279411456797245
72-73	0.0015030106551930772
74-75	5.30426401998491E-4
76-77	8.581927693313469E-4
78-79	1.250575827639837E-4
80-81	0.0021793290084743894
82-83	0.0025656479358020165
84-85	0.00391772175930986
86-87	0.004158707362852763
88-89	0.07090525286264826
90-91	6.81482540334209E-4
92-93	3.401758573111956E-5
94-95	4.075446291934814E-5
96-97	0.008320705953069892
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	319.0
20-21	1176.0
22-23	2417.0
24-25	4754.0
26-27	5977.0
28-29	10667.0
30-31	28493.0
32-33	36445.0
34-35	50738.0
36-37	80461.0
38-39	94585.0
40-41	99272.0
42-43	111156.0
44-45	129102.0
46-47	130843.0
48-49	125132.0
50-51	114867.0
52-53	103704.0
54-55	111940.0
56-57	118676.0
58-59	124629.0
60-61	132671.0
62-63	122817.0
64-65	128144.0
66-67	138936.0
68-69	141627.0
70-71	144331.0
72-73	138130.0
74-75	137442.0
76-77	138964.0
78-79	150954.0
80-81	157501.0
82-83	152409.0
84-85	153876.0
86-87	164124.0
88-89	180356.0
90-91	201540.0
92-93	219763.0
94-95	286753.0
96-97	358388.0
98-99	1104725.0
100-101	6902141.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.86350030891913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.89746095217573	76.2901320528601
2	6.9738095392262505	11.836437759729401
3	1.6962887293542914	4.318589973227219
4	0.6711314678155886	2.2781826210517426
5	0.3021876244927492	1.282234978224598
6	0.15221681697364145	0.7750591136559184
7	0.08328194928358205	0.4947318410128257
8	0.05357077554900685	0.3636962821881744
9	0.036267562257862734	0.277001305278649
>10	0.12888776864781276	1.6714741926782148
>50	0.0036744791362840327	0.21599653877523825
>100	0.0011737804656594673	0.16774354491907914
>500	4.85546215461333E-5	0.028719796398852512
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.848711378944026E-6	0.0
2	0.0	1.5697422757888052E-5	0.0	7.848711378944026E-6	0.0
3	0.0	1.5697422757888052E-5	0.0	7.848711378944026E-6	0.0
4	0.0	3.9243556894720134E-5	0.0	1.5697422757888052E-5	0.0
5	0.0	3.9243556894720134E-5	0.0	1.5697422757888052E-5	0.0
6	0.0	3.9243556894720134E-5	0.0	1.5697422757888052E-5	7.848711378944026E-6
7	0.0	3.9243556894720134E-5	0.0	1.5697422757888052E-5	2.354613413683208E-5
8	0.0	3.9243556894720134E-5	0.0	1.5697422757888052E-5	2.354613413683208E-5
9	0.0	5.4940979652608187E-5	0.0	1.5697422757888052E-5	4.709226827366416E-5
10-11	0.0	5.4940979652608187E-5	0.0	1.5697422757888052E-5	5.101662396313617E-5
12-13	0.0	5.4940979652608187E-5	0.0	2.354613413683208E-5	5.4940979652608187E-5
14-15	0.0	9.418453654732833E-5	0.0	2.354613413683208E-5	8.241146947891227E-5
16-17	0.0	1.0203324792627234E-4	0.0	3.1394845515776105E-5	9.418453654732831E-5
18-19	0.0	1.0988195930521637E-4	0.0	5.494097965260818E-5	1.1380631499468839E-4
20-21	0.0	1.177306706841604E-4	0.0	6.671404672102422E-5	1.5697422757888054E-4
22-23	0.0	1.4520116051046448E-4	0.0	1.8444471740518463E-4	1.6482293895782457E-4
24-25	0.0	1.726716503367686E-4	0.0	8.712069630627869E-4	1.6482293895782457E-4
26-27	0.0	1.805203617157126E-4	0.0	0.0025900747550515286	1.6482293895782457E-4
28-29	0.0	1.844447174051846E-4	0.0	0.015607162577030197	1.687472946472966E-4
30-31	0.0	1.8836907309465663E-4	0.0	0.023240034393053263	1.805203617157126E-4
32-33	0.0	1.8836907309465663E-4	0.0	0.02686221469443593	1.8836907309465663E-4
34-35	7.848711378944026E-6	2.1583956292096072E-4	0.0	0.03185007077575486	1.8836907309465663E-4
36-37	7.848711378944026E-6	2.4331005274726483E-4	0.0	0.0395143374372937	1.9621778447360066E-4
38-39	7.848711378944026E-6	2.7470489826304095E-4	0.0	0.054678047821413556	1.9621778447360066E-4
40-41	7.848711378944026E-6	3.02175388089345E-4	0.0	0.08686561318646302	1.9621778447360066E-4
42-43	7.848711378944026E-6	3.217971665367051E-4	0.0	0.10050274920737826	2.0014214016307266E-4
44-45	7.848711378944026E-6	3.4141894498406514E-4	0.0	0.11982235226664897	2.040664958525447E-4
46-47	7.848711378944026E-6	3.610407234314252E-4	0.0	0.14325860444417585	2.040664958525447E-4
48-49	7.848711378944026E-6	3.610407234314252E-4	0.0	0.16377513598873553	2.1191520723148872E-4
50-51	7.848711378944026E-6	3.7673814618931326E-4	0.0	0.18580646882943141	2.1191520723148872E-4
52-53	7.848711378944026E-6	3.7673814618931326E-4	0.0	0.21287667437540936	2.1583956292096072E-4
54-55	7.848711378944026E-6	4.0813299170508943E-4	0.0	0.27102385262631623	2.1976391861043275E-4
56-57	7.848711378944026E-6	4.2383041446297743E-4	0.0	0.3281036061296866	2.629318311946249E-4
58-59	7.848711378944026E-6	4.3167912584192144E-4	0.0	0.38827967627205046	2.9040232102092896E-4
60-61	7.848711378944026E-6	4.4737654859980955E-4	0.0	0.4238657336641827	3.1002409946828907E-4
62-63	7.848711378944026E-6	4.6307397135769755E-4	0.0	0.4416626867159382	3.453433006735372E-4
64-65	7.848711378944026E-6	4.944688168734737E-4	0.0	0.4594714128347622	3.610407234314252E-4
66-67	7.848711378944026E-6	5.023175282524177E-4	0.0	0.4688820177781161	3.845868575682573E-4
68-69	7.848711378944026E-6	5.101662396313617E-4	0.0	0.47788448972976494	3.885112132577293E-4
70-71	7.848711378944026E-6	5.180149510103058E-4	0.0	0.48682809634607166	4.0028428032614537E-4
72-73	7.848711378944026E-6	5.180149510103058E-4	0.0	0.49472782434897883	4.3560348153139344E-4
74-75	7.848711378944026E-6	5.258636623892498E-4	0.0	0.5017838158786494	4.434521929103375E-4
76-77	7.848711378944026E-6	5.651072192839699E-4	0.0	0.5084905397519572	4.6699832704716956E-4
78-79	7.848711378944026E-6	5.651072192839699E-4	0.0	0.5147263409425282	4.709226827366416E-4
80-81	7.848711378944026E-6	5.651072192839699E-4	0.0	0.5192432743411105	4.748470384261136E-4
82-83	7.848711378944026E-6	5.729559306629139E-4	0.0	0.5227516483274984	4.8269574980505767E-4
84-85	7.848711378944026E-6	5.808046420418579E-4	0.0	0.5258636623892498	4.8662010549452967E-4
86-87	7.848711378944026E-6	5.88653353420802E-4	0.0	0.5263856016959496	4.905444611840017E-4
88-89	7.848711378944026E-6	5.88653353420802E-4	0.0	0.5264758618768074	4.983931725629457E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15305	0.0	45.274246	1
GTATCAA	26985	0.0	34.767223	1
ATCAACG	33715	0.0	27.159584	3
TCAACGC	33875	0.0	27.006716	4
CAACGCA	34420	0.0	26.591194	5
AACGCAG	34995	0.0	26.225672	6
TATCAAC	35830	0.0	25.882406	2
ACGCAGA	41245	0.0	22.191027	7
CGCAGAG	41620	0.0	21.98108	8
GTACATG	41765	0.0	20.030817	1
TACATGG	41615	0.0	19.692753	2
ACATGGG	42305	0.0	18.957699	3
GCAGAGT	49370	0.0	18.513664	9
AGAGTAC	44620	0.0	18.33342	10-11
GAGTACT	25035	0.0	17.048937	12-13
CATGGGG	28075	0.0	16.582233	4
CAGAGTA	48400	0.0	16.428402	10-11
AGTACTT	28105	0.0	16.001518	12-13
GTACTTT	28960	0.0	14.789634	14-15
GTGGTAT	7515	0.0	14.684126	1
>>END_MODULE