FastQCFastQC Report
Fri 27 May 2016
SRR2139311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8515910
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91900.044.6454851
GTATCAA160600.033.6301081
TCAACGC197400.026.8102864
ATCAACG197500.026.7967113
CAACGCA200700.026.3278685
AACGCAG203800.025.9069146
TATCAAC213800.025.027642
ACGCAGA237250.022.2542867
CGCAGAG240250.022.011148
GTACATG217850.020.1365341
TACATGG222750.019.0752222
ACATGGG222900.018.6312663
AGAGTAC254900.018.42913610-11
GCAGAGT288500.018.3443689
GAGTACT168250.016.62078712-13
CAGAGTA281200.016.5348110-11
AGTACTT187750.015.794867512-13
CATGGGG166800.015.7642694
GTACTTT188700.014.74342414-15
GTATAAG38050.013.382731
Produced by FastQC (version 0.11.5)