FastQCFastQC Report
Fri 27 May 2016
SRR2139310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12199322
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125050.045.1705741
GTATCAA218300.032.869671
ATCAACG264100.026.3794163
TCAACGC263550.026.339494
CAACGCA270300.025.6817365
AACGCAG278150.024.9719416
TATCAAC287650.024.5824932
ACGCAGA321150.021.6283517
CGCAGAG325600.021.3840038
GTACATG313600.018.6372871
TACATGG308800.018.6027552
AGAGTAC348500.017.99174510-11
ACATGGG307650.017.926343
GCAGAGT398900.017.4127469
GAGTACT216650.016.87758412-13
CAGAGTA380700.016.25082210-11
CATGGGG215550.015.5605554
AGTACTT249700.015.1031312-13
TATACTG72650.014.6427155
GTACTTT250850.014.55238414-15
Produced by FastQC (version 0.11.5)