##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139310_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12199322
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896945748296503	31.0	31.0	34.0	30.0	34.0
2	32.04008034216984	33.0	31.0	34.0	30.0	34.0
3	32.10718275982878	33.0	31.0	34.0	30.0	34.0
4	35.80164217322897	37.0	35.0	37.0	35.0	37.0
5	35.672277934790145	37.0	35.0	37.0	33.0	37.0
6	35.67028192222486	37.0	35.0	37.0	33.0	37.0
7	35.55253283748064	37.0	35.0	37.0	33.0	37.0
8	35.54390670235608	37.0	35.0	37.0	33.0	37.0
9	37.15625524106996	39.0	37.0	39.0	33.0	39.0
10-11	37.0298569871342	39.0	37.0	39.0	33.0	39.0
12-13	36.99491988161309	39.0	37.0	39.0	33.0	39.0
14-15	38.16914411309088	40.0	37.0	41.0	33.0	41.0
16-17	38.16538423200896	40.0	37.0	41.0	33.0	41.0
18-19	38.188913203537055	40.0	37.5	41.0	33.0	41.0
20-21	38.17275386057662	40.0	37.5	41.0	33.0	41.0
22-23	38.084805621134585	40.0	37.0	41.0	33.0	41.0
24-25	37.99507388612402	40.0	37.0	41.0	33.0	41.0
26-27	37.839247785110416	40.0	37.0	41.0	32.5	41.0
28-29	37.70889156377487	40.0	37.0	41.0	32.0	41.0
30-31	37.57549770730415	39.0	37.0	41.0	32.0	41.0
32-33	37.72821928682031	40.0	37.0	41.0	33.0	41.0
34-35	37.696811757311025	40.0	37.0	41.0	32.5	41.0
36-37	37.57039959482777	40.0	36.5	41.0	32.0	41.0
38-39	37.413319355479516	39.0	36.0	41.0	31.0	41.0
40-41	37.35977689093777	39.0	36.0	41.0	31.0	41.0
42-43	37.25336163807217	39.0	36.0	40.5	31.0	41.0
44-45	37.32012221759261	39.0	36.0	40.5	31.5	41.0
46-47	37.47318241606037	39.5	36.0	41.0	32.0	41.0
48-49	37.40786614754498	39.0	36.0	41.0	31.0	41.0
50-51	37.274063133036464	39.0	36.0	41.0	31.0	41.0
52-53	37.1406706528976	39.0	36.0	41.0	31.0	41.0
54-55	37.01711042671468	39.0	35.0	40.5	31.0	41.0
56-57	36.87721046298351	39.0	35.0	40.0	31.0	41.0
58-59	36.74497793998928	38.5	35.0	40.0	31.0	41.0
60-61	36.51444796322673	38.0	35.0	40.0	30.0	41.0
62-63	36.2564903685513	38.0	34.5	40.0	30.0	41.0
64-65	36.036204803471	38.0	34.0	40.0	29.5	41.0
66-67	35.77401702497268	37.0	34.0	40.0	29.0	41.0
68-69	35.48590922687994	37.0	34.0	39.0	29.0	41.0
70-71	35.17619111437151	36.0	34.0	39.0	29.0	40.5
72-73	34.84436224568867	36.0	33.5	39.0	29.0	40.0
74-75	34.50145055100187	35.0	33.0	38.0	28.0	40.0
76-77	33.37615047177012	34.5	31.5	37.0	26.5	39.0
78-79	33.754736622796784	35.0	32.0	37.0	28.0	39.0
80-81	33.62687698412682	35.0	32.0	36.0	28.0	39.0
82-83	33.38237104477152	35.0	32.0	36.0	28.0	37.5
84-85	33.1630768079188	35.0	32.0	35.5	28.0	37.0
86-87	32.93908269741044	34.0	32.0	35.0	27.0	37.0
88-89	32.705098594001406	34.0	32.0	35.0	27.0	36.0
90-91	32.56237267044526	34.0	32.0	35.0	27.0	36.0
92-93	32.4837304832796	34.0	32.0	35.0	27.0	36.0
94-95	32.441169026163564	34.0	31.5	35.0	27.0	35.0
96-97	32.33984568128057	34.0	31.0	35.0	27.0	35.0
98-99	32.49018817043253	34.0	31.0	35.0	27.0	35.0
100-101	31.72016781121674	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	13.0
20	72.0
21	522.0
22	2405.0
23	8242.0
24	22095.0
25	47088.0
26	84719.0
27	134325.0
28	193916.0
29	259377.0
30	332840.0
31	414573.0
32	514240.0
33	646254.0
34	835785.0
35	1113309.0
36	1594552.0
37	2354023.0
38	2476812.0
39	1122183.0
40	41976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.037695865393175	22.45896124391175	15.190262212932817	24.313080677762255
2	13.889856190170777	22.938148132846205	40.975177409613075	22.196818267369945
3	16.735585797309064	27.985022446329396	31.67392417381884	23.6054675825427
4	11.870118683644879	19.779443480547528	39.98264821602381	28.36778961978379
5	12.388376993409961	38.664238881472265	36.57114715063673	12.37623697448104
6	28.42373535184988	40.075612398787406	19.178172360726276	12.322479888636433
7	24.97980625480662	33.16357253296536	24.779024604810004	17.07759660741802
8	23.51433956739563	37.858726903019694	21.77298049842442	16.853953031160255
9	25.073122916175176	16.461800090201734	21.757569805928558	36.707507187694524
10-11	23.45838153956425	27.283450670455295	30.026000625280652	19.23216716469981
12-13	24.352345154919266	24.84547911760998	29.900395284262522	20.90178044320824
14-15	21.58456019113193	26.233449694991247	27.15458695163551	25.027403162241313
16-17	21.278944846279163	29.2514616795917	27.847715635344322	21.621877838784812
18-19	21.32878537268571	28.60832584520736	29.248070834146905	20.814817947960023
20-21	22.10970051484252	27.827359805557766	28.997499668916504	21.065440010683208
22-23	21.83527320475263	27.78734632345394	28.878790447647244	21.498590024146186
24-25	21.62697342916484	27.94021239201479	28.873837683561277	21.558976495259095
26-27	21.701553127140052	28.028699644859817	28.744849515788033	21.52489771221209
28-29	21.662353213550958	28.08465731287802	28.77275223563336	21.480237237937665
30-31	21.87035537431728	27.941599689825498	28.68850768294946	21.499537252907764
32-33	21.667100177379737	28.089447454218636	28.69358540320285	21.54986696519878
34-35	21.81134277641506	28.0955343005417	28.668543155490973	21.424579767552263
36-37	21.815454461981762	28.073207487270135	28.60524081679009	21.506097233958013
38-39	21.67387469995773	28.142672016645864	28.659934991084903	21.523518292311504
40-41	21.860622024743893	27.99719627246183	28.608990972118566	21.53319073067571
42-43	21.755254945928144	28.144502492819807	28.388620392001073	21.711622169250976
44-45	21.826741718920808	28.20855620696005	28.238529051614165	21.726173022504973
46-47	21.937463801208274	28.184110499140917	28.026244943199934	21.852180756450874
48-49	21.862536426939467	28.271579749034387	28.01375009878196	21.852133725244194
50-51	21.88236671703391	28.34882925963862	27.90753821100534	21.861265812322134
52-53	21.97749032968335	28.245677681251045	27.88332691253102	21.89350507653458
54-55	21.967505247175385	28.194910913787286	27.900023234774775	21.93756060426255
56-57	21.98256911390176	28.257468008541366	27.862628224589326	21.897334652967547
58-59	21.946026515278305	28.28190755074469	27.878376802681025	21.893689131295982
60-61	22.021663940871857	28.283810657887937	27.867139165673766	21.827386235566436
62-63	21.996626459753312	28.31305064821658	27.820224184201297	21.870098707828806
64-65	21.94552121978125	28.24729939354385	27.794847620103198	22.0123317665717
66-67	22.012831305354542	28.260885784323612	27.728045870662747	21.9982370396591
68-69	21.95928765707472	28.235608538261232	27.797633754639655	22.007470050024388
70-71	21.94212936587747	28.08560515105866	27.853722759437012	22.11854272362686
72-73	22.003179631141165	28.13092664765935	27.801206586132825	22.064687135066656
74-75	21.898240499868525	28.219301536037417	27.7915523671522	22.09090559694186
76-77	21.969610750360324	28.157185391296412	27.768624774519697	22.104579083823566
78-79	21.924590613904858	28.141825892777593	27.755003277839833	22.17858021547772
80-81	21.93479964834001	28.173823168180554	27.79125882965402	22.10011835382541
82-83	21.96555870750079	28.151203320777313	27.83125553714966	22.051982434572242
84-85	21.998207574837846	28.15941398337164	27.782622751795493	22.05975568999502
86-87	21.88762617405761	28.202491122324798	27.85942267371472	22.050460029902872
88-89	21.908849608648016	28.1576562557444	27.799140712494015	22.134353423113566
90-91	21.925678868279878	28.158094443762128	27.860531132927573	22.05569555503042
92-93	21.879484374280274	28.138108241680243	27.896164915611628	22.08624246842786
94-95	21.680257672414765	28.208899104102986	27.968071698303742	22.142771525178503
96-97	21.633742437527864	28.28876161424797	28.039449894880438	22.03804605334373
98-99	21.78068876027175	28.89310649307641	28.141332839886836	21.184871906765
100-101	25.208493677975376	36.26222310279098	29.021926650903257	9.507356568330387
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2714.0
1	2481.0
2	3356.5
3	4676.0
4	6036.0
5	9087.0
6	11596.5
7	13263.0
8	14129.5
9	13670.0
10	12759.0
11	12073.0
12	13498.0
13	17923.0
14	27946.0
15	41810.0
16	52887.0
17	58684.5
18	60062.5
19	58840.0
20	56707.5
21	55704.0
22	57994.5
23	66063.5
24	80501.0
25	101654.0
26	127992.5
27	155458.5
28	184605.0
29	215227.0
30	243716.0
31	272684.5
32	303074.5
33	332672.5
34	361161.0
35	388003.5
36	415641.0
37	441486.5
38	462698.5
39	478473.5
40	490677.0
41	502841.5
42	510744.5
43	515727.5
44	521843.0
45	525934.0
46	525535.0
47	521026.5
48	514467.0
49	501535.0
50	483212.5
51	463480.0
52	438333.5
53	409660.5
54	379775.5
55	347428.5
56	315010.5
57	281258.0
58	245635.0
59	209496.5
60	173644.0
61	140740.0
62	111518.5
63	86282.0
64	64594.5
65	47791.5
66	35640.0
67	26861.0
68	20305.0
69	15107.0
70	11182.0
71	8254.0
72	6034.5
73	4362.5
74	3130.5
75	2158.5
76	1497.5
77	1045.0
78	718.0
79	491.5
80	318.5
81	209.5
82	162.5
83	116.5
84	76.0
85	52.5
86	38.0
87	31.0
88	20.0
89	13.0
90	9.0
91	5.5
92	4.5
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0035575747570233823
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	6.557741487600704E-5
20-21	0.0019018238367631813
22-23	3.976250552319648E-4
24-25	4.100151111069199E-5
26-27	3.6504851597319E-4
28-29	0.0035623247386503327
30-31	4.5197966683181733E-4
32-33	7.824601428187434E-5
34-35	0.0023254889867237293
36-37	0.05572097973504593
38-39	0.03111107380065432
40-41	0.04106831824142768
42-43	9.161653948297478E-4
44-45	0.0023970852128693
46-47	0.0015918671507269362
48-49	6.556281612750866E-4
50-51	4.415338727788142E-6
52-53	0.0010340928373782485
54-55	0.0023850739730692746
56-57	0.0013233153661061099
58-59	0.0038942315671928046
60-61	0.0016046141447155454
62-63	1.4112455667484577E-4
64-65	8.181787132541432E-4
66-67	4.4283195850857083E-4
68-69	1.8522281646792666E-4
70-71	0.0012149600700409663
72-73	0.00172171999827828
74-75	6.439367800108637E-4
76-77	0.0010995471972156587
78-79	1.511003203483102E-4
80-81	0.0021000975249319135
82-83	0.0024714909489434566
84-85	0.003601089820728473
86-87	0.0038870116144572434
88-89	0.06644756390442363
90-91	7.194001560925683E-4
92-93	7.059396171018814E-5
94-95	6.03244478035778E-5
96-97	0.008289518939490882
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	288.0
20-21	1136.0
22-23	2277.0
24-25	4159.0
26-27	6116.0
28-29	10685.0
30-31	25933.0
32-33	33189.0
34-35	47295.0
36-37	74866.0
38-39	88610.0
40-41	92066.0
42-43	102980.0
44-45	119885.0
46-47	121063.0
48-49	116905.0
50-51	108441.0
52-53	105034.0
54-55	113543.0
56-57	119515.0
58-59	123522.0
60-61	123382.0
62-63	117561.0
64-65	121089.0
66-67	128875.0
68-69	132876.0
70-71	135635.0
72-73	128989.0
74-75	130005.0
76-77	131776.0
78-79	142932.0
80-81	146610.0
82-83	142526.0
84-85	144903.0
86-87	153943.0
88-89	168228.0
90-91	184361.0
92-93	202042.0
94-95	262234.0
96-97	337559.0
98-99	1049063.0
100-101	6697225.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.42413186492848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.06475655488543	76.93703640327215
2	6.9366832345166065	11.851202866611704
3	1.6755454696639966	4.293960514387823
4	0.6044582908710574	2.06541298984874
5	0.2730012325030061	1.1660446642312396
6	0.15478617023309316	0.7933484530115411
7	0.08663114619826186	0.5180273319515153
8	0.05708865227332248	0.3901398847829879
9	0.03251334955088526	0.2499682193464802
>10	0.11084092793395843	1.4522748244236703
>50	0.002989245594201447	0.17199490909936244
>100	7.057257761664068E-4	0.11058893903282731
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.639435371900176E-5	0.0	0.0	0.0
3	0.0	1.639435371900176E-5	0.0	0.0	0.0
4	0.0	4.0985884297504405E-5	0.0	0.0	0.0
5	0.0	4.0985884297504405E-5	0.0	0.0	0.0
6	0.0	4.0985884297504405E-5	0.0	0.0	0.0
7	0.0	4.0985884297504405E-5	0.0	0.0	0.0
8	0.0	4.918306115700528E-5	0.0	0.0	0.0
9	8.19717685950088E-6	7.377459173550793E-5	0.0	2.459153057850264E-5	0.0
10-11	3.278870743800352E-5	7.377459173550793E-5	0.0	3.688729586775396E-5	0.0
12-13	3.278870743800352E-5	7.377459173550793E-5	0.0	4.0985884297504405E-5	0.0
14-15	3.278870743800352E-5	9.016894545450968E-5	0.0	4.0985884297504405E-5	1.639435371900176E-5
16-17	3.278870743800352E-5	9.016894545450968E-5	0.0	5.738023801650616E-5	1.639435371900176E-5
18-19	3.278870743800352E-5	1.02464710743761E-4	0.0	6.557741487600704E-5	2.459153057850264E-5
20-21	4.0985884297504405E-5	1.2705624132226364E-4	0.0	6.557741487600704E-5	2.459153057850264E-5
22-23	4.0985884297504405E-5	1.4754918347101585E-4	0.0	7.377459173550793E-5	3.278870743800352E-5
24-25	4.0985884297504405E-5	1.516477719007663E-4	0.0	9.426753388426014E-5	4.0985884297504405E-5
26-27	4.0985884297504405E-5	1.5574636033051674E-4	0.0	1.3115482975201408E-4	4.0985884297504405E-5
28-29	5.7380238016506165E-5	1.5574636033051674E-4	0.0	3.1968989752053436E-4	4.0985884297504405E-5
30-31	5.7380238016506165E-5	1.7214071404951848E-4	0.0	0.0010902245223136172	5.3281649586755724E-5
32-33	6.557741487600704E-5	1.7214071404951848E-4	0.0	0.0028403217818170554	6.557741487600704E-5
34-35	6.557741487600704E-5	2.2132377520652375E-4	0.0	0.005360953666113576	6.557741487600704E-5
36-37	7.377459173550793E-5	2.7870401322302994E-4	0.0	0.00950462656859127	6.557741487600704E-5
38-39	7.377459173550793E-5	3.032955438015326E-4	0.0	0.015947607580158965	6.557741487600704E-5
40-41	7.377459173550793E-5	3.360842512395361E-4	0.0	0.02316932039337924	6.557741487600704E-5
42-43	8.197176859500881E-5	3.606757818180388E-4	0.0	0.030046751778500476	6.557741487600704E-5
44-45	8.197176859500881E-5	4.303517851237962E-4	0.0	0.037961126036348575	6.967600330575748E-5
46-47	8.197176859500881E-5	4.754362578510511E-4	0.0	0.046678823626427765	8.197176859500881E-5
48-49	8.197176859500881E-5	4.795348462808015E-4	0.0	0.05526946497518469	9.016894545450968E-5
50-51	8.197176859500881E-5	5.246193190080563E-4	0.0	0.0642412750479084	9.016894545450968E-5
52-53	8.197176859500881E-5	5.246193190080563E-4	0.0	0.07234008578509526	9.836612231401056E-5
54-55	8.607035702475925E-5	5.246193190080563E-4	0.0	0.08104138902145547	1.0656329917351145E-4
56-57	9.016894545450968E-5	5.328164958675573E-4	0.0	0.08947628400988186	1.0656329917351145E-4
58-59	9.016894545450968E-5	5.410136727270581E-4	0.0	0.09819398159996104	1.1066188760326189E-4
60-61	9.016894545450968E-5	5.410136727270581E-4	0.0	0.10593621514375963	1.229576528925132E-4
62-63	1.1476047603301233E-4	5.49210849586559E-4	0.0	0.11322350537185591	1.3115482975201408E-4
64-65	1.1476047603301233E-4	6.229854413220669E-4	0.0	0.12089606291234874	1.3525341818176453E-4
66-67	1.1476047603301233E-4	6.393797950410686E-4	0.0	0.1288719159966431	1.434505950412654E-4
68-69	1.1476047603301233E-4	6.557741487600705E-4	0.0	0.13752403617184628	1.4754918347101585E-4
70-71	1.1476047603301233E-4	6.721685024790722E-4	0.0	0.14624173376192545	1.4754918347101585E-4
72-73	1.1476047603301233E-4	6.762670909088227E-4	0.0	0.15405364330902982	1.7214071404951848E-4
74-75	1.1885906446276276E-4	6.967600330575747E-4	0.0	0.16087369445613453	1.7214071404951848E-4
76-77	1.229576528925132E-4	7.131543867765766E-4	0.0	0.1674437317090245	1.7623930247926892E-4
78-79	1.229576528925132E-4	7.213515636360774E-4	0.0	0.17332110751728663	1.8033789090901936E-4
80-81	1.3115482975201408E-4	7.213515636360774E-4	0.0	0.17764101972224355	1.8853506776852024E-4
82-83	1.3115482975201408E-4	7.213515636360774E-4	0.0	0.1806411864528209	1.9673224462802113E-4
84-85	1.3525341818176453E-4	7.213515636360774E-4	0.0	0.1835265927073652	1.9673224462802113E-4
86-87	1.3935200661151497E-4	7.213515636360774E-4	0.0	0.18405121202637326	1.9673224462802113E-4
88-89	1.3935200661151497E-4	7.623374479335818E-4	0.0	0.18411678944124926	2.0083083305777157E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12505	0.0	45.170574	1
GTATCAA	21830	0.0	32.86967	1
ATCAACG	26410	0.0	26.379416	3
TCAACGC	26355	0.0	26.33949	4
CAACGCA	27030	0.0	25.681736	5
AACGCAG	27815	0.0	24.971941	6
TATCAAC	28765	0.0	24.582493	2
ACGCAGA	32115	0.0	21.628351	7
CGCAGAG	32560	0.0	21.384003	8
GTACATG	31360	0.0	18.637287	1
TACATGG	30880	0.0	18.602755	2
AGAGTAC	34850	0.0	17.991745	10-11
ACATGGG	30765	0.0	17.92634	3
GCAGAGT	39890	0.0	17.412746	9
GAGTACT	21665	0.0	16.877584	12-13
CAGAGTA	38070	0.0	16.250822	10-11
CATGGGG	21555	0.0	15.560555	4
AGTACTT	24970	0.0	15.10313	12-13
TATACTG	7265	0.0	14.642715	5
GTACTTT	25085	0.0	14.552384	14-15
>>END_MODULE