FastQCFastQC Report
Fri 27 May 2016
SRR2139309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22484887
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA263900.047.4983061
GTATCAA470400.034.354961
ATCAACG574150.027.3694023
TCAACGC577050.027.253374
CAACGCA586550.026.833135
AACGCAG598950.026.3467046
TATCAAC612200.025.9778862
ACGCAGA699700.022.4955887
CGCAGAG705750.022.3262048
GTACATG627550.020.6344951
TACATGG633550.020.0401062
ACATGGG640800.019.3301073
GCAGAGT834350.018.7364129
AGAGTAC748750.018.0764110-11
GAGTACT488150.017.67037212-13
CAGAGTA816100.017.07658410-11
CATGGGG455400.016.8941544
GTGGTAT130600.016.2449721
AGTACTT537150.015.86965212-13
GTACTTT563900.015.28207414-15
Produced by FastQC (version 0.11.5)