FastQCFastQC Report
Fri 27 May 2016
SRR2139308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24772143
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA324050.040.763051
GTATCAA537100.030.4784891
ATCAACG636450.024.9819263
TCAACGC640450.024.8518754
CAACGCA651750.024.3763265
AACGCAG665650.023.9922626
TATCAAC680550.023.5829012
ACGCAGA762000.020.8789587
CGCAGAG770150.020.658018
GTACATG653250.020.2782171
TACATGG658100.019.470682
ACATGGG664750.018.7067683
AGAGTAC772150.017.7154510-11
GCAGAGT908650.017.3538429
GAGTACT503500.017.28709612-13
CATGGGG475000.016.4082364
CAGAGTA882700.016.29786510-11
AGTACTT562600.015.27888712-13
GTACTTT583300.014.88582814-15
ATGGGGG298700.013.6033315
Produced by FastQC (version 0.11.5)