FastQCFastQC Report
Fri 27 May 2016
SRR2139307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21997728
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA247500.048.8822561
GTATCAA445800.035.134211
TCAACGC542800.028.0537344
ATCAACG546000.027.9198193
CAACGCA554600.027.441835
AACGCAG570900.026.7385586
TATCAAC583700.026.405162
ACGCAGA664150.022.8864617
CGCAGAG671100.022.6804718
GTACATG593800.020.3604261
TACATGG601700.019.5665262
ACATGGG600750.019.0678793
GCAGAGT791450.019.0108859
AGAGTAC719600.018.0826910-11
GAGTACT463850.017.83547412-13
CAGAGTA782300.017.22696710-11
CATGGGG432700.016.4941334
AGTACTT518800.015.76175712-13
GTACTTT536850.015.45616414-15
GTGGTAT121650.015.0103561
Produced by FastQC (version 0.11.5)