##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139307_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21997728
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.347814874336112	31.0	31.0	34.0	30.0	34.0
2	31.5087878166327	31.0	31.0	34.0	30.0	34.0
3	31.54925113175324	31.0	31.0	34.0	30.0	34.0
4	35.36812833579904	37.0	35.0	37.0	33.0	37.0
5	35.1394059422864	37.0	35.0	37.0	32.0	37.0
6	35.16864823494499	37.0	35.0	37.0	32.0	37.0
7	35.05064591215966	37.0	35.0	37.0	32.0	37.0
8	35.02229366596405	37.0	35.0	37.0	32.0	37.0
9	36.55165869857105	39.0	35.0	39.0	32.0	39.0
10-11	36.45049488747202	38.0	35.0	39.0	32.0	39.0
12-13	36.379805996328344	38.0	35.0	39.0	32.0	39.0
14-15	37.43092341172688	39.0	36.0	41.0	32.0	41.0
16-17	37.416384523892646	39.0	36.0	41.0	32.0	41.0
18-19	37.42839555975963	39.0	36.0	41.0	32.0	41.0
20-21	37.40422073604073	39.0	36.0	41.0	32.0	41.0
22-23	37.31091893111646	39.0	36.0	41.0	31.0	41.0
24-25	37.20769118875073	39.0	36.0	41.0	31.0	41.0
26-27	36.98494435365103	39.0	36.0	40.0	31.0	41.0
28-29	36.80777101688926	39.0	35.5	40.0	30.0	41.0
30-31	36.64724198837686	38.5	35.0	40.0	30.0	41.0
32-33	36.4487092027218	38.0	35.0	40.0	30.0	41.0
34-35	36.28972940677964	38.0	35.0	40.0	30.0	41.0
36-37	36.14181424738451	38.0	35.0	40.0	30.0	41.0
38-39	35.982710921490366	38.0	34.0	40.0	29.0	41.0
40-41	35.83564121636407	38.0	34.0	40.0	29.0	41.0
42-43	35.68406814429919	38.0	34.0	40.0	28.0	41.0
44-45	35.518786925258695	38.0	33.5	40.0	27.5	41.0
46-47	35.81768204210634	38.0	34.0	40.0	28.5	41.0
48-49	36.20131124346312	38.0	34.5	40.0	29.0	41.0
50-51	36.21572346003872	38.0	34.5	40.0	29.0	41.0
52-53	36.12355344502282	38.0	34.0	40.0	29.0	41.0
54-55	36.01199915579332	38.0	34.0	40.0	28.5	41.0
56-57	35.86758345543098	38.0	34.0	40.0	28.0	41.0
58-59	35.69612634891416	38.0	34.0	40.0	28.0	41.0
60-61	35.508200351501664	37.5	34.0	40.0	28.0	41.0
62-63	35.289504066071245	37.0	33.0	40.0	28.0	41.0
64-65	34.989772825753406	37.0	33.0	39.5	27.5	41.0
66-67	34.72258483123456	36.0	32.5	39.0	27.0	41.0
68-69	34.461886276345425	36.0	32.0	39.0	26.5	40.5
70-71	34.19432183925065	35.5	32.0	39.0	26.0	40.0
72-73	33.904148590779855	35.0	32.0	38.0	26.0	40.0
74-75	33.58655267406999	35.0	31.5	37.5	26.0	39.5
76-77	31.864156996444898	33.5	29.5	35.5	25.0	38.5
78-79	32.93677893361807	34.0	31.0	36.0	26.0	39.0
80-81	33.00903590194938	34.0	31.0	36.0	26.0	39.0
82-83	32.7920855244085	34.0	31.0	36.0	26.0	37.5
84-85	32.576583732990485	34.0	31.0	35.0	26.0	37.0
86-87	32.37125786917193	34.0	31.0	35.0	26.0	37.0
88-89	32.19563114172856	34.0	31.0	35.0	26.0	36.0
90-91	32.09679863261195	34.0	31.0	35.0	26.0	36.0
92-93	32.04709751511477	34.0	31.0	35.0	26.0	36.0
94-95	32.054200853583176	34.0	31.0	35.0	26.0	35.0
96-97	32.15106743781199	34.0	31.0	35.0	26.0	35.0
98-99	32.33668953657539	34.0	31.0	35.0	27.0	35.0
100	30.81310280296233	32.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	12.0
19	45.0
20	354.0
21	2411.0
22	10888.0
23	35304.0
24	88563.0
25	176209.0
26	296412.0
27	441511.0
28	594964.0
29	748283.0
30	902656.0
31	1062641.0
32	1246088.0
33	1471078.0
34	1775956.0
35	2180157.0
36	2790060.0
37	3521214.0
38	3288267.0
39	1328427.0
40	36228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.921390972740454	21.745391160396203	14.679365978159197	23.653851888704143
2	14.661585020447191	22.573405218984973	40.90907580552166	21.855933955046176
3	17.657441713980642	27.735714342863044	31.241508213939184	23.365335729217126
4	12.425832657846692	19.3348892823943	39.60282067401437	28.63645738574464
5	12.54441367763071	38.68053555348989	36.315568589628896	12.45948217925051
6	28.541520287913368	39.75762406008475	19.1841221057011	12.516733546300781
7	25.06226097531527	33.27566828719766	24.58112492344664	17.080945814040433
8	23.676076911215556	37.67521354932655	21.718729316045728	16.929980223412162
9	25.259776827861497	16.605137585117884	21.780394775314978	36.354690811705645
10-11	23.561467347900656	27.263242822167815	29.828291812681744	19.34699801724978
12-13	24.487071404392186	24.895155732970174	29.762437856972635	20.85533500566501
14-15	21.702859742104337	26.214108662326367	27.190379777264567	24.892651818304724
16-17	21.268313756404773	29.228931038534185	27.872127590557493	21.63062761450355
18-19	21.31597181308906	28.520588580784345	29.399108853423407	20.764330752703188
20-21	22.125867925166492	27.742263459363954	29.19431849302987	20.937550122439685
22-23	21.841782342403576	27.669411794422416	29.087604146131373	21.401201717042635
24-25	21.64130609365632	27.82496853742978	29.08245924922236	21.451266119691546
26-27	21.68232946524826	27.884319338984813	28.980780098832128	21.4525710969348
28-29	21.629027632745824	27.944156048489944	29.00127243402741	21.425543884736825
30-31	21.884387360051395	27.815042606351746	28.86727896105119	21.433291072545668
32-33	21.68740415532907	28.040923490221715	28.78885042751229	21.482821926936925
34-35	21.826646973854857	28.040752857881333	28.780598668607983	21.352001499655824
36-37	21.841774963487364	27.965716865938166	28.723231128234016	21.469277042340455
38-39	21.70778215140959	28.0665246518688	28.762188529910215	21.463504666811396
40-41	21.921409871666448	27.935875004591654	28.639468158976538	21.50324696476536
42-43	21.803067636941485	28.07181604115413	28.41973094533648	21.705385376567904
44-45	21.898152467409478	28.17272733710513	28.25095010591216	21.678170089573236
46-47	21.967826726078943	28.161614571870867	28.0192068047108	21.851351897339384
48-49	21.895667896472567	28.27999804302203	28.003607650561353	21.82072640994405
50-51	21.94251561955296	28.36019942623419	27.88553870965894	21.811746244553905
52-53	22.060614266600144	28.24000882512466	27.845371885775865	21.85400502249933
54-55	22.00325410021185	28.179822351863926	27.894537482094815	21.92238606582941
56-57	22.01337628864424	28.266596578932596	27.874324732412862	21.8457024000103
58-59	21.992376757266737	28.292870553560377	27.8543396324003	21.86041305677259
60-61	22.033441487203813	28.285032303043874	27.870395259606894	21.81113095014541
62-63	22.029649097421082	28.34275715521033	27.814768733688517	21.812825013680076
64-65	22.066639179278667	28.29147884855978	27.74894019343747	21.892941778724083
66-67	22.035940163723183	28.301979153116978	27.731378870467143	21.930701812692696
68-69	21.977081943797447	28.30291439815295	27.794343593304266	21.925660064745333
70-71	21.952811813420684	28.133405780985964	27.847900117822476	22.065882287770876
72-73	22.007346042905667	28.194000804958403	27.782158104698052	22.01649504743788
74-75	21.849219230267565	28.308648955533698	27.81763467722344	22.024497136975295
76-77	21.9730529023083	28.26083406565938	27.812159505054296	21.953953526978022
78-79	21.911213060231756	28.2643105100579	27.769531557078263	22.054944872632085
80-81	21.921123026396184	28.27629671627304	27.798438896247063	22.004141361083715
82-83	21.949872419885164	28.25090788247517	27.868577536606487	21.93064216103318
84-85	21.942306339250074	28.285841209831	27.857206327726473	21.914646123192455
86-87	21.84688677934168	28.30339400269079	27.922480365388946	21.92723885257858
88-89	21.866928810437773	28.279494800276872	27.898194114973148	21.955382274312203
90-91	21.88503085591147	28.30253641667071	27.931662724249417	21.880770003168397
92-93	21.87417192991945	28.301892630771373	27.94714301660106	21.876792422708114
94-95	21.669145438507037	28.332612293301256	28.065930747487293	21.932311520704406
96-97	21.708554935298057	28.454391196315953	28.189491405070992	21.647562463314998
98-99	22.704618736940457	30.433005200737952	28.48068272727461	18.381693335046982
100	22.15451745926441	31.06200947622743	22.232769338205017	24.550703726303137
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5586.0
1	5010.0
2	6907.0
3	9829.0
4	12725.5
5	20521.0
6	27505.0
7	32018.0
8	34706.5
9	33675.5
10	30459.5
11	26878.5
12	27906.5
13	35673.5
14	55738.5
15	84591.0
16	108339.5
17	122737.0
18	128770.5
19	127598.5
20	122751.0
21	117379.0
22	116445.5
23	126524.5
24	149251.0
25	184879.5
26	231121.0
27	280205.0
28	329501.5
29	383284.5
30	434769.5
31	482527.0
32	534397.5
33	584598.5
34	631889.0
35	676532.5
36	722314.0
37	766173.5
38	799136.5
39	825007.5
40	850768.0
41	873591.5
42	887133.5
43	899237.5
44	909116.0
45	915823.0
46	916456.0
47	911453.0
48	902044.5
49	881654.0
50	857846.0
51	828661.0
52	788003.5
53	740783.5
54	693346.0
55	643465.5
56	590406.5
57	533283.5
58	472070.0
59	407763.5
60	340456.0
61	276536.5
62	221063.5
63	172939.0
64	130333.0
65	98461.0
66	74984.0
67	57624.5
68	44712.5
69	33117.0
70	24243.5
71	18163.5
72	13336.0
73	9461.5
74	6742.5
75	4893.5
76	3488.0
77	2416.0
78	1683.5
79	1195.5
80	856.5
81	614.5
82	469.5
83	344.5
84	250.0
85	193.0
86	151.0
87	115.0
88	76.0
89	48.0
90	33.5
91	24.0
92	16.5
93	8.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.1282268787031097
3	0.0
4	3.1821468107979156E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.005882425675960717
14-15	6.81888602313839E-4
16-17	0.001652443379607203
18-19	0.0
20-21	3.864313584592063E-5
22-23	2.341742857809328E-4
24-25	4.867139376585857E-4
26-27	4.551516234086392E-5
28-29	0.009559596648342095
30-31	0.0029244154265787984
32-33	0.0076889394491673054
34-35	0.006718535381314858
36-37	0.009064082115420298
38-39	0.0030968987609412447
40-41	4.2060927496728475E-5
42-43	2.543127857472001E-4
44-45	0.0015065919815277565
46-47	7.902805959714953E-4
48-49	8.974729780588716E-5
50-51	0.0
52-53	2.7158780476935345E-5
54-55	2.4908524068073107E-5
56-57	0.0
58-59	0.0010923397187702806
60-61	0.0
62-63	0.0
64-65	0.0
66-67	6.599218414013813E-4
68-69	0.0014481804819061917
70-71	0.001517901915241932
72-73	6.297291386479594E-4
74-75	1.5315629931719857E-4
76-77	0.004162655715685876
78-79	0.0021044624312052088
80-81	1.6505187537008178E-4
82-83	8.817174303720971E-6
84-85	5.07250580115634E-4
86-87	0.0011642016717450922
88-89	1.4504088100670222E-4
90-91	6.298377641842693E-6
92-93	0.0
94-95	3.3133491989746377E-6
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	944.0
20-21	3203.0
22-23	6527.0
24-25	12498.0
26-27	17012.0
28-29	26085.0
30-31	53508.0
32-33	66799.0
34-35	92477.0
36-37	126282.0
38-39	155786.0
40-41	159617.0
42-43	183688.0
44-45	222621.0
46-47	209754.0
48-49	188791.0
50-51	178161.0
52-53	174385.0
54-55	188726.0
56-57	197903.0
58-59	203764.0
60-61	205303.0
62-63	198076.0
64-65	205779.0
66-67	219162.0
68-69	228661.0
70-71	232994.0
72-73	226809.0
74-75	231953.0
76-77	211744.0
78-79	238504.0
80-81	254400.0
82-83	254530.0
84-85	260742.0
86-87	281469.0
88-89	315178.0
90-91	354916.0
92-93	401615.0
94-95	503526.0
96-97	983419.0
98-99	1604414.0
100-101	1.2116003E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.629728902048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.32805375589177	74.70480918952799
2	7.188498090343027	12.023442930165544
3	1.8243820811207696	4.577177365736524
4	0.7310279705296124	2.445426839808233
5	0.3456923528635308	1.44550788767441
6	0.18820095158122654	0.9443516735907262
7	0.11194830516864814	0.6553544488608449
8	0.0681822210376538	0.45616485290548014
9	0.048551757031874455	0.3654333250464388
>10	0.16178684737878604	2.0859063054199285
>50	0.002761216706014285	0.15087456959041962
>100	8.641519510683901E-4	0.10998357714097104
>500	4.0255145911426415E-5	0.02597521336569482
>1k	1.0043250155570172E-5	0.009591821166742065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.545924015425593E-6	0.0
2	0.0	3.182146810797915E-5	0.0	4.545924015425593E-6	0.0
3	0.0	3.182146810797915E-5	0.0	4.545924015425593E-6	0.0
4	0.0	5.909701220053271E-5	0.0	9.091848030851186E-6	9.091848030851186E-6
5	0.0	5.909701220053271E-5	0.0	9.091848030851186E-6	9.091848030851186E-6
6	0.0	5.909701220053271E-5	0.0	1.363777204627678E-5	1.363777204627678E-5
7	0.0	5.909701220053271E-5	0.0	2.2729620077127965E-5	1.363777204627678E-5
8	0.0	6.36429362159583E-5	0.0	2.2729620077127965E-5	1.363777204627678E-5
9	0.0	1.0455625235478864E-4	0.0	3.636739212340474E-5	1.363777204627678E-5
10-11	0.0	1.0682921436250144E-4	0.0	5.0005164169681525E-5	1.363777204627678E-5
12-13	0.0	1.1364810038563983E-4	0.0	7.728070826223508E-5	1.363777204627678E-5
14-15	2.2729620077127965E-6	1.7729103660159813E-4	0.0	7.728070826223508E-5	2.045665806941517E-5
16-17	4.545924015425593E-6	1.8638288463244932E-4	0.0	8.182663227766067E-5	2.2729620077127965E-5
18-19	4.545924015425593E-6	2.000206566787261E-4	0.0	9.091848030851186E-5	2.2729620077127965E-5
20-21	4.545924015425593E-6	2.1365842872500288E-4	0.0	1.0682921436250144E-4	2.2729620077127965E-5
22-23	1.1364810038563983E-5	2.3638804880213082E-4	0.0	1.2046698640877821E-4	2.5002582084840762E-5
24-25	2.045665806941517E-5	2.6820951691011E-4	0.0	1.3637772046276779E-4	2.9548506100266353E-5
26-27	2.2729620077127965E-5	2.8184728895638676E-4	0.0	1.7956399860931093E-4	3.4094430115691946E-5
28-29	2.2729620077127965E-5	2.8184728895638676E-4	0.0	3.5458207320319626E-4	3.636739212340474E-5
30-31	2.9548506100266353E-5	3.136687570643659E-4	0.0	0.0011137513837792701	3.636739212340474E-5
32-33	3.182146810797915E-5	3.182146810797915E-4	0.0	0.002782105497440463	3.636739212340474E-5
34-35	3.4094430115691946E-5	3.6140095922633465E-4	0.0	0.005061886391176398	4.545924015425593E-5
36-37	3.636739212340474E-5	4.045872373728778E-4	0.0	0.008316767986221123	5.0005164169681525E-5
38-39	3.636739212340474E-5	4.3413574347314415E-4	0.0	0.014117367029904179	5.0005164169681525E-5
40-41	5.0005164169681525E-5	4.864138696505385E-4	0.0	0.020167991894435643	5.0005164169681525E-5
42-43	5.0005164169681525E-5	4.977786796891025E-4	0.0	0.025932223545995295	5.0005164169681525E-5
44-45	5.9097012200532705E-5	5.500568058664967E-4	0.0	0.032526086330370116	5.0005164169681525E-5
46-47	6.59158982236711E-5	5.909701220053271E-4	0.0	0.03970410035072713	5.227812617739432E-5
48-49	7.273478424680949E-5	5.909701220053271E-4	0.0	0.04684120105494531	6.36429362159583E-5
50-51	7.273478424680949E-5	6.659778682598493E-4	0.0	0.05392147770897067	6.36429362159583E-5
52-53	7.273478424680949E-5	6.773426782984134E-4	0.0	0.06090401699666438	6.818886023138389E-5
54-55	7.500774625452228E-5	6.864345263292645E-4	0.0	0.06815249283926049	7.273478424680949E-5
56-57	7.728070826223508E-5	6.909804503446901E-4	0.0	0.07457588347305685	7.273478424680949E-5
58-59	7.728070826223508E-5	6.932534123524029E-4	0.0	0.08102200372693034	7.273478424680949E-5
60-61	7.728070826223508E-5	7.023452603832541E-4	0.0	0.08744312139871899	7.273478424680949E-5
62-63	7.728070826223508E-5	7.182559944372437E-4	0.0	0.09386196610849992	7.273478424680949E-5
64-65	7.728070826223508E-5	7.773530066377764E-4	0.0	0.09954209816577421	7.273478424680949E-5
66-67	7.728070826223508E-5	7.978096647071917E-4	0.0	0.10550407751200488	7.273478424680949E-5
68-69	7.728070826223508E-5	8.114474367534683E-4	0.0	0.11176608784325362	7.273478424680949E-5
70-71	7.728070826223508E-5	8.205392847843195E-4	0.0	0.1170211760050856	7.273478424680949E-5
72-73	7.728070826223508E-5	8.296311328151707E-4	0.0	0.12143981414807929	7.273478424680949E-5
74-75	7.728070826223508E-5	8.569066769077243E-4	0.0	0.1250106374621961	7.273478424680949E-5
76-77	7.728070826223508E-5	8.910011070234163E-4	0.0	0.12814277910882432	7.273478424680949E-5
78-79	7.728070826223508E-5	9.091848030851186E-4	0.0	0.1302702715480435	7.273478424680949E-5
80-81	7.728070826223508E-5	9.114577650928314E-4	0.0	0.131065808250743	7.955367026994788E-5
82-83	7.728070826223508E-5	9.137307271005442E-4	0.0	0.13166587022077916	8.637255629308627E-5
84-85	7.728070826223508E-5	9.137307271005442E-4	0.0	0.13192271492765073	8.637255629308627E-5
86-87	7.728070826223508E-5	9.27368499146821E-4	0.0	0.13210909781228314	9.091848030851186E-5
88	7.728070826223508E-5	9.364603471776722E-4	0.0	0.13212728150834485	9.546440432393746E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24750	0.0	48.882256	1
GTATCAA	44580	0.0	35.13421	1
TCAACGC	54280	0.0	28.053734	4
ATCAACG	54600	0.0	27.919819	3
CAACGCA	55460	0.0	27.44183	5
AACGCAG	57090	0.0	26.738558	6
TATCAAC	58370	0.0	26.40516	2
ACGCAGA	66415	0.0	22.886461	7
CGCAGAG	67110	0.0	22.680471	8
GTACATG	59380	0.0	20.360426	1
TACATGG	60170	0.0	19.566526	2
ACATGGG	60075	0.0	19.067879	3
GCAGAGT	79145	0.0	19.010885	9
AGAGTAC	71960	0.0	18.08269	10-11
GAGTACT	46385	0.0	17.835474	12-13
CAGAGTA	78230	0.0	17.226967	10-11
CATGGGG	43270	0.0	16.494133	4
AGTACTT	51880	0.0	15.761757	12-13
GTACTTT	53685	0.0	15.456164	14-15
GTGGTAT	12165	0.0	15.010356	1
>>END_MODULE