FastQCFastQC Report
Fri 27 May 2016
SRR2139306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22160805
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA240050.046.9907841
GTATCAA419950.034.856821
TCAACGC512000.027.7603724
ATCAACG514150.027.6851083
CAACGCA524250.027.0796765
AACGCAG539800.026.3929086
TATCAAC553500.026.0136682
ACGCAGA627800.022.683527
CGCAGAG635950.022.386228
GTACATG577000.020.0085091
TACATGG568450.019.784532
ACATGGG587250.018.6134243
GCAGAGT763900.018.439069
AGAGTAC684150.018.11907610-11
GAGTACT448150.017.67852412-13
CAGAGTA745000.016.92938410-11
CATGGGG424600.016.5988884
GTGGTAT119200.015.7602511
AGTACTT501900.015.68907612-13
GTACTTT517200.015.2116814-15
Produced by FastQC (version 0.11.5)