##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139306_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22160805
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.344965311503802	31.0	31.0	34.0	30.0	34.0
2	31.49759708638743	31.0	31.0	34.0	30.0	34.0
3	31.536554876955055	31.0	31.0	34.0	30.0	34.0
4	35.35390027573457	37.0	35.0	37.0	33.0	37.0
5	35.12450423168292	37.0	35.0	37.0	32.0	37.0
6	35.152753972610654	37.0	35.0	37.0	32.0	37.0
7	35.03271000308879	37.0	35.0	37.0	32.0	37.0
8	35.005064391839554	37.0	35.0	37.0	32.0	37.0
9	36.53390050587061	39.0	35.0	39.0	32.0	39.0
10-11	36.4314015443031	38.0	35.0	39.0	32.0	39.0
12-13	36.36192543547042	38.0	35.0	39.0	32.0	39.0
14-15	37.413273773222585	39.0	36.0	41.0	32.0	41.0
16-17	37.39483809365228	39.0	36.0	41.0	32.0	41.0
18-19	37.40330622014859	39.0	36.0	41.0	32.0	41.0
20-21	37.37612505238965	39.0	36.0	41.0	31.5	41.0
22-23	37.28386886763936	39.0	36.0	41.0	31.0	41.0
24-25	37.17924342256249	39.0	36.0	41.0	31.0	41.0
26-27	36.9521196850517	39.0	36.0	40.0	30.5	41.0
28-29	36.77531469669357	39.0	35.5	40.0	30.0	41.0
30-31	36.6127484092773	38.5	35.0	40.0	30.0	41.0
32-33	36.41684442913399	38.0	35.0	40.0	30.0	41.0
34-35	36.25541669881594	38.0	35.0	40.0	30.0	41.0
36-37	36.10848576382428	38.0	34.5	40.0	29.5	41.0
38-39	35.94518076706132	38.0	34.0	40.0	29.0	41.0
40-41	35.79790870698669	38.0	34.0	40.0	29.0	41.0
42-43	35.642713614919614	38.0	34.0	40.0	28.0	41.0
44-45	35.477833480505865	38.0	33.5	40.0	27.0	41.0
46-47	35.77014479382444	38.0	34.0	40.0	28.5	41.0
48-49	36.15740138127106	38.0	34.0	40.0	29.0	41.0
50-51	36.170280135719864	38.0	34.0	40.0	29.0	41.0
52-53	36.07559628689388	38.0	34.0	40.0	28.5	41.0
54-55	35.961136128455536	38.0	34.0	40.0	28.0	41.0
56-57	35.816667139238064	38.0	34.0	40.0	28.0	41.0
58-59	35.64010691834605	38.0	34.0	40.0	28.0	41.0
60-61	35.449491242679564	37.5	33.5	40.0	28.0	41.0
62-63	35.22662918900082	37.0	33.0	40.0	28.0	41.0
64-65	34.922313898402	36.5	33.0	39.5	27.0	41.0
66-67	34.6516622335015	36.0	32.5	39.0	26.5	41.0
68-69	34.38783032597317	36.0	32.0	39.0	26.0	40.0
70-71	34.119809176803145	35.0	32.0	39.0	26.0	40.0
72-73	33.82489432277967	35.0	32.0	38.0	26.0	40.0
74-75	33.505860782241335	35.0	31.0	37.5	26.0	39.5
76-77	31.789958542096187	33.5	29.5	35.5	25.0	38.5
78-79	32.86088258017777	34.0	31.0	36.0	26.0	39.0
80-81	32.93614364859113	34.0	31.0	36.0	26.0	38.5
82-83	32.721990564399675	34.0	31.0	36.0	26.0	37.5
84-85	32.50718969494014	34.0	31.0	35.0	26.0	37.0
86-87	32.302121220885994	34.0	31.0	35.0	26.0	36.5
88-89	32.13101005787636	34.0	31.0	35.0	26.0	36.0
90-91	32.034514059129755	34.0	31.0	35.0	26.0	36.0
92-93	31.98805140232061	34.0	31.0	35.0	26.0	35.5
94-95	32.000067159238405	34.0	31.0	35.0	26.0	35.0
96-97	32.10154258235578	34.0	31.0	35.0	26.0	35.0
98-99	32.29315827637654	34.0	31.0	35.0	27.0	35.0
100	30.75509816630673	32.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	8.0
19	57.0
20	352.0
21	2645.0
22	11902.0
23	38245.0
24	94032.0
25	187054.0
26	312136.0
27	459835.0
28	617858.0
29	774175.0
30	925497.0
31	1084556.0
32	1264942.0
33	1492617.0
34	1795543.0
35	2203878.0
36	2823736.0
37	3554422.0
38	3255274.0
39	1231511.0
40	30528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.03164596231951	21.632891945937885	14.551754776056194	23.78370731568641
2	14.786501544820673	22.507252402399242	40.80559928930387	21.90064676347621
3	17.765135336915787	27.55857921226237	31.185699255961147	23.4905861948607
4	12.50897304989476	19.228589359844523	39.53023545535622	28.732202134904494
5	12.59067980608105	38.63915593318925	36.31285054852475	12.45731371220495
6	28.742376461504897	39.683928449350105	19.086662239932167	12.487032849212833
7	25.22589319295937	33.19602785187632	24.44087658368006	17.137202371484246
8	23.674753692386176	37.73432869428706	21.61377260437967	16.977145008947105
9	25.22085276234324	16.574573892961016	21.642589247096396	36.56198409759934
10-11	23.635964487752137	27.180271655294106	29.81536546167885	19.3683983952749
12-13	24.518617091668045	24.770420870870534	29.72610661428466	20.984855423176764
14-15	21.74718122684114	26.14018431445218	27.075325665496297	25.037308793210382
16-17	21.38105813366122	29.164067061202015	27.745975323385203	21.70889948175156
18-19	21.431762068210066	28.462077528320833	29.210759717438062	20.895400686031035
20-21	22.206965905878604	27.69776184333607	29.024434084229306	21.070838166556012
22-23	21.926315285846535	27.600305483124178	28.905692918144695	21.56768631288459
24-25	21.742698062598958	27.761225036738807	28.907427507328627	21.588649393333608
26-27	21.780940218276857	27.845114952531958	28.794048906328474	21.57989592286271
28-29	21.761663467122098	27.906790123819686	28.782630685428508	21.54891572362971
30-31	22.012935433461234	27.754509154568357	28.68869314273767	21.543862269232736
32-33	21.83479117715866	27.92793549381254	28.62985362941273	21.60741969961607
34-35	21.974141359167383	27.918469482622726	28.62353086408622	21.483858294123664
36-37	21.95992746746258	27.877648115941938	28.573441778018065	21.588982638577416
38-39	21.83554650806185	27.97405710523208	28.62180383888332	21.568592547822753
40-41	22.02591860600363	27.87129894274557	28.489118802771042	21.613663648479754
42-43	21.917494162674764	27.962851890542616	28.30913126821013	21.810522678572493
44-45	22.011021995038753	28.083781814775616	28.121454585090973	21.78374160509466
46-47	22.0608727511559	28.052683163150427	27.926990858851585	21.959453226842083
48-49	21.984748063888226	28.16235422299671	27.935433093575778	21.917464619539288
50-51	22.038592625528278	28.216314603727987	27.81904936919682	21.926043401546917
52-53	22.129442404549035	28.136681048160582	27.768506558782168	21.96536998850821
54-55	22.09253360111671	28.069168199028837	27.822060380256193	22.01623781959826
56-57	22.040422835929128	28.17824681196312	27.813917071722337	21.967413280385415
58-59	22.08636369474798	28.206202301154654	27.76924250656359	21.938191497533772
60-61	22.146013229222973	28.19169586915777	27.771586699407365	21.890704202211893
62-63	22.09595188329443	28.232708695761378	27.744337359820047	21.92700206112415
64-65	22.12628334585198	28.164568860654448	27.73245300388686	21.97669478960671
66-67	22.127966167485415	28.195644083492972	27.65665740650575	22.01973234251586
68-69	22.053110014441874	28.22714354600184	27.71020737492172	22.009539064634566
70-71	22.05878187920943	28.038576132961225	27.783496228134236	22.11914575969511
72-73	22.084045543340643	28.1192432761896	27.714705931134674	22.082005249335083
74-75	21.961817537042364	28.216411005356495	27.743633425379254	22.078138032221883
76-77	22.053110882977222	28.15679168115131	27.744386655092164	22.045710780779306
78-79	22.008836892085824	28.16656297125501	27.711374767353337	22.11322536930583
80-81	22.006157519319608	28.17744730660338	27.751487606649576	22.06490756742743
82-83	22.007217177661612	28.145989390627047	27.837509236893577	22.00928419481777
84-85	22.05603926208007	28.16922586382419	27.78562686205101	21.989108012044724
86-87	21.9306273591992	28.20050995899679	27.86356172483087	22.00530095697314
88-89	21.967964400724664	28.17416976476582	27.845817312394956	22.01204852211456
90-91	21.96649400690067	28.210061468773418	27.89098163000665	21.93246289431926
92-93	21.946163050492	28.18387626161327	27.909106716460542	21.960853971434183
94-95	21.760941721526297	28.216843326897294	28.03795636299776	21.984258588578648
96-97	21.791467420245016	28.368854765949532	28.11978119184852	21.719896621956934
98-99	22.794052090564666	30.374350490996232	28.403728815895736	18.427868602543363
100	22.271638032411413	31.027062560053164	22.13899026314032	24.562309144395098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5531.0
1	4912.5
2	6495.5
3	9091.0
4	11728.0
5	18958.0
6	25668.0
7	29876.0
8	32131.0
9	31122.0
10	28123.0
11	24668.0
12	25656.5
13	32823.0
14	51252.5
15	78152.0
16	100009.5
17	112929.0
18	118165.5
19	117102.0
20	113064.0
21	108211.5
22	107638.0
23	117652.0
24	139587.0
25	174618.0
26	220715.0
27	268554.0
28	314079.0
29	364414.5
30	413742.5
31	461328.0
32	514631.0
33	565577.5
34	616505.5
35	665003.5
36	713235.0
37	760364.0
38	795794.0
39	822887.0
40	850366.5
41	874560.0
42	889156.5
43	903089.5
44	915447.0
45	925835.5
46	928460.0
47	923777.0
48	915047.0
49	893672.5
50	866942.5
51	837555.5
52	800559.5
53	756023.5
54	706957.5
55	654121.5
56	600640.0
57	545053.0
58	482363.0
59	416764.0
60	349921.5
61	284242.0
62	225944.0
63	175593.0
64	132397.5
65	99776.0
66	75440.5
67	57667.5
68	44464.0
69	32939.0
70	23974.5
71	17700.0
72	13053.5
73	9470.0
74	6760.5
75	4816.0
76	3419.5
77	2367.5
78	1640.0
79	1190.0
80	880.5
81	633.5
82	458.0
83	308.5
84	211.0
85	156.0
86	115.5
87	81.5
88	49.0
89	35.0
90	29.0
91	23.5
92	18.0
93	9.5
94	3.5
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.1269764342946928
3	0.0
4	4.512471455797747E-6
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0058143194707953975
14-15	6.520521253627745E-4
16-17	0.001764376339216919
18-19	0.0
20-21	2.9333275629515223E-5
22-23	2.0988379817366343E-4
24-25	4.379762723871064E-4
26-27	5.6474988447476364E-5
28-29	0.009602007744323337
30-31	0.002952422392384996
32-33	0.0077665339055391115
34-35	0.006850379314996218
36-37	0.00905877229490872
38-39	0.0031598534683496865
40-41	3.0096663305641083E-5
42-43	2.51806695554024E-4
44-45	0.0014859163112184001
46-47	8.025172449203093E-4
48-49	1.0779520549443181E-4
50-51	0.0
52-53	3.895354988812784E-5
54-55	2.4542658601163687E-5
56-57	0.0
58-59	0.0011070185299658546
60-61	0.0
62-63	0.0
64-65	0.0
66-67	6.365432407590744E-4
68-69	0.001347455755318425
70-71	0.0013948325360072034
72-73	6.370837927549756E-4
74-75	1.8222214631143755E-4
76-77	0.003855389910772078
78-79	0.0019594722684713036
80-81	1.5225309629932215E-4
82-83	2.287155389651657E-5
84-85	4.6399264803649186E-4
86-87	0.0010333200071360902
88-89	1.1676709634962105E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1032.0
20-21	3275.0
22-23	6358.0
24-25	12581.0
26-27	16869.0
28-29	25420.0
30-31	50480.0
32-33	62827.0
34-35	86987.0
36-37	117807.0
38-39	143996.0
40-41	148101.0
42-43	170398.0
44-45	205254.0
46-47	192296.0
48-49	174953.0
50-51	165111.0
52-53	161489.0
54-55	175613.0
56-57	184627.0
58-59	188863.0
60-61	191652.0
62-63	185609.0
64-65	193750.0
66-67	206183.0
68-69	214712.0
70-71	220253.0
72-73	215765.0
74-75	221102.0
76-77	198906.0
78-79	224529.0
80-81	243381.0
82-83	246744.0
84-85	252613.0
86-87	275419.0
88-89	311539.0
90-91	350485.0
92-93	403994.0
94-95	514209.0
96-97	1021365.0
98-99	1669713.0
100-101	1.2504545E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.29170624064822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.36288038508664	74.43186781852822
2	7.069755230421292	11.77703951691073
3	1.8521511915179267	4.628064988715332
4	0.7272059780781457	2.4228090681011274
5	0.34463632250428394	1.435267366694206
6	0.19974880349040144	0.9982451197346093
7	0.12292194549583686	0.7166865002337751
8	0.08016813295344416	0.5341872463855601
9	0.055769726311279987	0.4180640094936435
>10	0.18033346086151217	2.2928353612753867
>50	0.0037443650124258147	0.20778584757459415
>100	6.224005379853439E-4	0.08662589907662646
>500	4.142026680175989E-5	0.025562419464545662
>1k	2.0637462037083224E-5	0.02495883781168575
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.024942911595495E-6	0.0	0.0	0.0
3	0.0	1.3537414367393243E-5	0.0	1.804988582319099E-5	0.0
4	0.0	5.8662128925370713E-5	0.0	3.609977164638198E-5	0.0
5	4.512471455797747E-6	5.8662128925370713E-5	0.0	3.609977164638198E-5	0.0
6	1.3537414367393243E-5	5.8662128925370713E-5	0.0	4.5124714557977475E-5	4.512471455797747E-6
7	1.3537414367393243E-5	5.8662128925370713E-5	0.0	4.9637186013775224E-5	9.024942911595495E-6
8	1.804988582319099E-5	5.8662128925370713E-5	0.0	5.414965746957297E-5	9.024942911595495E-6
9	2.2562357278988738E-5	9.024942911595495E-5	0.0	8.122448620435945E-5	9.024942911595495E-6
10-11	4.738095028587635E-5	9.024942911595495E-5	0.0	9.024942911595495E-5	9.024942911595495E-6
12-13	5.8662128925370713E-5	9.024942911595495E-5	0.0	9.701813629965156E-5	1.1281178639494369E-5
14-15	7.671201474856171E-5	1.2183672930653918E-4	0.0	1.286054364902358E-4	2.4818593006887612E-5
16-17	9.927437202755045E-5	1.2183672930653918E-4	0.0	1.5116779376922451E-4	3.158730019058423E-5
18-19	1.1281178639494369E-4	1.353741436739324E-4	0.0	1.5793650095292117E-4	3.158730019058423E-5
20-21	1.286054364902358E-4	1.5116779376922451E-4	0.0	1.7598638677611216E-4	3.158730019058423E-5
22-23	1.466553223134268E-4	2.0306121551089864E-4	2.2562357278988737E-6	1.8501132968770764E-4	3.609977164638198E-5
24-25	1.872675654156065E-4	2.14342394150393E-4	4.512471455797747E-6	2.2562357278988737E-4	4.5124714557977475E-5
26-27	2.0757368696669638E-4	2.2111110133408963E-4	4.512471455797747E-6	2.820294659873592E-4	4.5124714557977475E-5
28-29	2.0757368696669638E-4	2.2111110133408963E-4	6.768707183696621E-6	6.407709467232801E-4	4.5124714557977475E-5
30-31	2.1885486560619074E-4	2.865419374431569E-4	9.024942911595495E-6	0.002770657473859817	4.738095028587635E-5
32-33	2.391609871572806E-4	2.9105440889895474E-4	9.024942911595495E-6	0.0061550110657081275	4.9637186013775224E-5
34-35	2.6397958016416824E-4	3.4746030209642655E-4	9.024942911595495E-6	0.009498752414454258	5.414965746957297E-5
36-37	2.8428570171525806E-4	4.016099595659995E-4	9.024942911595495E-6	0.014399296415450612	5.414965746957297E-5
38-39	2.8428570171525806E-4	4.219160811170894E-4	9.024942911595495E-6	0.022142697433599548	5.414965746957297E-5
40-41	3.1136053045004457E-4	4.918593886819544E-4	9.024942911595495E-6	0.02934911434850855	5.414965746957297E-5
42-43	3.1136053045004457E-4	5.099092745051454E-4	9.024942911595495E-6	0.03601854716017762	5.414965746957297E-5
44-45	3.248979448174378E-4	5.843650535258082E-4	9.024942911595495E-6	0.043127945938786966	6.317460038116846E-5
46-47	3.3843535918483107E-4	6.407709467232801E-4	9.024942911595495E-6	0.05081945353519423	7.219954329276396E-5
48-49	3.3843535918483107E-4	6.407709467232801E-4	9.024942911595495E-6	0.058154475886593476	8.122448620435945E-5
50-51	3.3843535918483107E-4	6.97176839920752E-4	9.024942911595495E-6	0.06506758215687562	8.122448620435945E-5
52-53	3.5648524500802204E-4	6.994330756486508E-4	9.024942911595495E-6	0.07158584717477547	9.250566484385382E-5
54-55	3.655101879196175E-4	7.062017828323474E-4	9.024942911595495E-6	0.07760774033253756	1.0604307921124707E-4
56-57	3.655101879196175E-4	7.174829614718419E-4	9.024942911595495E-6	0.08342431603906086	1.0829931493914594E-4
58-59	3.813038380149097E-4	7.197391971997408E-4	9.024942911595495E-6	0.08903106182288956	1.1055555066704481E-4
60-61	3.92585016654404E-4	7.287641401113362E-4	9.024942911595495E-6	0.09462652642807876	1.1281178639494369E-4
62-63	4.038661952938984E-4	7.332766115671339E-4	9.024942911595495E-6	0.09997831757465489	1.1732425785074143E-4
64-65	4.128911382054939E-4	8.483446336899765E-4	9.024942911595495E-6	0.10512930374144802	1.1732425785074143E-4
66-67	4.1514737393339276E-4	8.483446336899765E-4	9.024942911595495E-6	0.11012460964301612	1.195804935786403E-4
68-69	4.2417231684498824E-4	8.663945195131674E-4	9.024942911595495E-6	0.1149710039865429	1.2183672930653918E-4
70-71	4.28684788300786E-4	8.912131125200552E-4	9.024942911595495E-6	0.11947445049942906	1.2183672930653918E-4
72-73	4.28684788300786E-4	9.002380554316506E-4	9.024942911595495E-6	0.12322882675065278	1.3988661512973017E-4
74-75	4.3094102402868486E-4	9.250566484385382E-4	9.024942911595495E-6	0.12613937083964233	1.3988661512973017E-4
76-77	4.331972597565838E-4	9.837187773639089E-4	9.024942911595495E-6	0.12884459747739307	1.4214285085762903E-4
78-79	4.331972597565838E-4	0.0010107936060986954	9.024942911595495E-6	0.1305751302806915	1.4439908658552792E-4
80-81	4.377097312123815E-4	0.001015306077554493	9.024942911595495E-6	0.13123169487751007	1.4439908658552792E-4
82-83	4.399659669402804E-4	0.0010175623132823918	9.024942911595495E-6	0.13182959734540328	1.4891155804132566E-4
84-85	4.4222220266817926E-4	0.0010310997276497852	9.024942911595495E-6	0.13208229574692798	1.4891155804132566E-4
86-87	4.4222220266817926E-4	0.0010378684348334819	9.024942911595495E-6	0.13222895106924137	1.4891155804132566E-4
88	4.4222220266817926E-4	0.001105555506670448	9.024942911595495E-6	0.13223797601215298	1.4891155804132566E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24005	0.0	46.990784	1
GTATCAA	41995	0.0	34.85682	1
TCAACGC	51200	0.0	27.760372	4
ATCAACG	51415	0.0	27.685108	3
CAACGCA	52425	0.0	27.079676	5
AACGCAG	53980	0.0	26.392908	6
TATCAAC	55350	0.0	26.013668	2
ACGCAGA	62780	0.0	22.68352	7
CGCAGAG	63595	0.0	22.38622	8
GTACATG	57700	0.0	20.008509	1
TACATGG	56845	0.0	19.78453	2
ACATGGG	58725	0.0	18.613424	3
GCAGAGT	76390	0.0	18.43906	9
AGAGTAC	68415	0.0	18.119076	10-11
GAGTACT	44815	0.0	17.678524	12-13
CAGAGTA	74500	0.0	16.929384	10-11
CATGGGG	42460	0.0	16.598888	4
GTGGTAT	11920	0.0	15.760251	1
AGTACTT	50190	0.0	15.689076	12-13
GTACTTT	51720	0.0	15.21168	14-15
>>END_MODULE