FastQCFastQC Report
Fri 27 May 2016
SRR2139305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10736432
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA194450.054.102321
GTATCAA365900.039.1627961
ATCAACG460350.030.6893463
TCAACGC460900.030.6527274
TATCAAC466850.030.3971582
CAACGCA469400.030.108025
AACGCAG483850.029.2088576
TGGTATC73350.026.5410062
GTGGTAT79500.025.9158521
ACGCAGA550650.025.6884047
CGCAGAG557000.025.418558
GCAGAGT632300.022.3652949
GAGTACT336200.020.61152812-13
GTACATG535900.020.5331941
TACATGG527300.020.4853252
ACATGGG533700.019.6645473
CAGAGTA626900.019.52942510-11
AGAGTAC600200.018.8848810-11
GTACTTT374700.018.4604414-15
AGTACTT361200.018.07912-13
Produced by FastQC (version 0.11.5)