##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10736432
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6614779472361	33.0	33.0	33.0	33.0	33.0
2	32.65757227354488	33.0	33.0	33.0	33.0	33.0
3	32.64669705913473	33.0	33.0	33.0	33.0	33.0
4	36.56458430510248	37.0	37.0	37.0	37.0	37.0
5	36.5873492236527	37.0	37.0	37.0	37.0	37.0
6	36.579164754175316	37.0	37.0	37.0	37.0	37.0
7	36.55211675536156	37.0	37.0	37.0	37.0	37.0
8	36.55187188816545	37.0	37.0	37.0	37.0	37.0
9	36.58067782667464	37.0	37.0	37.0	37.0	37.0
10-11	36.51894716978602	37.0	37.0	37.0	37.0	37.0
12-13	36.51805502051333	37.0	37.0	37.0	37.0	37.0
14-15	38.554984933542165	40.0	37.0	40.0	37.0	40.0
16-17	38.49407000388956	40.0	37.0	40.0	37.0	40.0
18-19	38.530835011109836	40.0	37.0	40.0	37.0	40.0
20-21	38.54043450685918	40.0	37.0	40.0	37.0	40.0
22-23	38.54754492892181	40.0	37.0	40.0	37.0	40.0
24-25	38.52552606522012	40.0	37.0	40.0	37.0	40.0
26-27	38.414273192927965	40.0	37.0	40.0	37.0	40.0
28-29	38.369286482767706	40.0	37.0	40.0	37.0	40.0
30-31	38.30020792363892	40.0	37.0	40.0	37.0	40.0
32-33	38.22826897345983	40.0	37.0	40.0	37.0	40.0
34-35	38.18782455375302	40.0	37.0	40.0	37.0	40.0
36-37	38.14747485945911	40.0	37.0	40.0	37.0	40.0
38-39	38.09273857728135	40.0	37.0	40.0	35.0	40.0
40-41	38.04500633146449	40.0	37.0	40.0	33.0	40.0
42-43	37.996390968839364	40.0	37.0	40.0	33.0	40.0
44-45	37.95837686227435	40.0	37.0	40.0	33.0	40.0
46-47	37.899832388095966	40.0	37.0	40.0	33.0	40.0
48-49	37.89664230193782	40.0	37.0	40.0	33.0	40.0
50-51	37.83429972144198	40.0	37.0	40.0	33.0	40.0
52-53	37.731600751469536	40.0	37.0	40.0	33.0	40.0
54-55	37.595022505212725	40.0	37.0	40.0	33.0	40.0
56-57	37.52145300457909	40.0	37.0	40.0	33.0	40.0
58-59	37.40113281716765	40.0	37.0	40.0	33.0	40.0
60-61	37.27372916054628	37.0	37.0	40.0	33.0	40.0
62-63	37.10692321915225	37.0	37.0	40.0	33.0	40.0
64-65	36.946211614346595	37.0	37.0	40.0	33.0	40.0
66-67	36.75304596752322	37.0	37.0	40.0	33.0	40.0
68-69	36.477532025001366	37.0	37.0	40.0	33.0	40.0
70-71	36.26010281291033	37.0	37.0	40.0	33.0	40.0
72-73	35.93773005398686	37.0	37.0	37.0	33.0	40.0
74-75	35.843712653613764	37.0	37.0	37.0	33.0	40.0
76-77	34.17397243028553	35.0	33.0	37.0	30.0	37.0
78-79	35.358637900010066	37.0	33.0	37.0	33.0	37.0
80-81	35.382458910689444	37.0	33.0	37.0	33.0	37.0
82-83	35.212784301888334	37.0	33.0	37.0	33.0	37.0
84-85	35.07006147842638	37.0	33.0	37.0	33.0	37.0
86-87	34.95788159702523	37.0	33.0	37.0	33.0	37.0
88-89	34.83820691478496	37.0	33.0	37.0	33.0	37.0
90-91	34.736605569913166	37.0	33.0	37.0	33.0	37.0
92-93	34.701091770041955	37.0	33.0	37.0	33.0	37.0
94-95	34.7031593011846	37.0	33.0	37.0	33.0	37.0
96-97	34.6843848636768	37.0	33.0	37.0	33.0	37.0
98-99	34.6827906554888	37.0	33.0	37.0	33.0	37.0
100	34.67441072267679	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	11.0
20	69.0
21	326.0
22	1230.0
23	3493.0
24	8068.0
25	16260.0
26	27879.0
27	45474.0
28	67668.0
29	97369.0
30	134384.0
31	184299.0
32	249827.0
33	342660.0
34	480631.0
35	708434.0
36	1246130.0
37	3017367.0
38	3959485.0
39	145365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.59485199552328	20.886436015242307	14.820100383442098	22.698611605792316
2	14.260137818597462	21.58899716404854	42.66870036526101	21.482164652092987
3	17.955713779028265	27.548556168380706	30.77599709102614	23.719732961564883
4	11.922666673621181	18.905200535894977	39.99107897297724	29.181053817506598
5	12.75432098857423	38.47970163644682	35.570960631986495	13.195016742992458
6	28.002217123901126	38.85563658392285	19.680504659276004	13.461641632900017
7	25.549959241580446	33.67116747910293	23.524528446694397	17.254344832622234
8	23.914257548504008	36.851441894290396	21.71532404806364	17.518976509141957
9	25.676719860676116	17.24312811102246	21.696175876842396	35.383976151459024
10-11	23.643643130848172	27.567034516675264	28.98269509121426	19.806627261262303
12-13	24.868313799575624	25.271022767519153	29.221154718007085	20.639508714898135
14-15	22.003789852938635	26.390412600690528	27.449157184078445	24.156640362292393
16-17	20.813180766198677	29.668878823057792	28.010073551436825	21.507866859306706
18-19	20.842268641947342	28.409004965523	30.281349520958173	20.467376871571485
20-21	22.19679933331902	27.358039568954272	30.079880910971085	20.365280186755623
22-23	21.70881573526312	27.44083166122192	29.943931444192867	20.906421159322093
24-25	21.235890545031815	27.689910043744813	30.097586733216737	20.97661267800664
26-27	21.1406231716832	27.86584195458125	30.031953065355804	20.961581808379748
28-29	20.97513738282273	27.937306103176418	30.133818179512563	20.953738334488293
30-31	21.37889426164427	27.725018729497148	30.006075680377485	20.890011328481098
32-33	21.158841326151208	27.891248625095105	29.92422257759441	21.025687471159276
34-35	21.20430592080768	27.90460349294766	29.935802326401433	20.955288259843226
36-37	21.140995877352108	27.935243067050227	29.875005802149186	21.04875525344848
38-39	21.01776816846519	27.953662667618328	29.938027961228673	21.09054120268781
40-41	21.28569358293089	27.87542499691163	29.816758954983502	21.022122465173975
42-43	21.272721447337506	27.928534083349643	29.575833603216818	21.222910866096033
44-45	21.330397300111187	28.153283746764174	29.282651605449058	21.233667347675585
46-47	21.441328409440533	28.11363650433484	28.908177771514982	21.536857314709643
48-49	21.391905130987134	28.26316106807302	28.81705565834568	21.527878142594158
50-51	21.391145525215574	28.389573461759554	28.66769465010493	21.55158636291994
52-53	21.49701983384649	28.366598738459786	28.585370273616867	21.551011154076857
54-55	21.563339858578786	28.337772138265606	28.501175145696557	21.597712857459054
56-57	21.600391067900524	28.40821394837649	28.389306572504232	21.602088411218762
58-59	21.45453223737843	28.46334903186612	28.553687424384822	21.528431306370628
60-61	21.592941682101966	28.536880531156754	28.378599895982816	21.491577890758464
62-63	21.514092754456062	28.56719280880531	28.371878684944214	21.54683575179442
64-65	21.46468541454299	28.55679823552755	28.35667993372532	21.621836416204136
66-67	21.594242949403462	28.516546076698614	28.208759739179257	21.680451234718667
68-69	21.664163625267722	28.472397961769314	28.231147085076124	21.632291327886833
70-71	21.55758027487426	28.2650773885618	28.376051126435968	21.80129121012797
72-73	21.676168191668467	28.25883559639944	28.283793068341712	21.78120314359039
74-75	21.569095529358147	28.429209016290603	28.202730224050903	21.798965230300347
76-77	21.655879961482952	28.361905973348357	28.125937333051503	21.85627673211719
78-79	21.71621171943183	28.332448299199843	28.089700208391	21.86163977297733
80-81	21.666931056974718	28.354722421482077	28.107280010050538	21.87106651149267
82-83	21.757355692809842	28.276594479002913	28.22003455926041	21.746015268926836
84-85	21.861919906061107	28.24582435514466	28.149444636685462	21.742811102108767
86-87	21.665951501933982	28.303048581189877	28.201460174715542	21.8295397421606
88-89	21.787731360579148	28.236955962049947	28.193993128900313	21.78131954847059
90-91	21.870243832329287	28.14427538551473	28.230379248463304	21.75510153369267
92-93	21.85231334443662	28.12188060714283	28.25542049027499	21.77038555814556
94-95	21.714439459550068	28.20074535358251	28.278048531378076	21.806766655489344
96-97	21.763193909693417	28.342783722960657	28.387108094031017	21.506914273314916
98-99	22.844720330573377	30.16043394664204	28.627041104175028	18.36780461860955
100	21.710123740406203	31.215132280657265	22.541161026620223	24.53358295231631
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2977.0
1	2579.5
2	3376.0
3	4925.0
4	6746.5
5	11082.5
6	14820.5
7	17796.0
8	20584.0
9	21070.0
10	19372.0
11	17493.0
12	18976.0
13	25102.5
14	39302.5
15	59014.5
16	75357.0
17	85497.5
18	87648.0
19	81532.5
20	71532.0
21	61306.5
22	54863.0
23	55624.5
24	63540.5
25	78542.5
26	99633.5
27	123483.5
28	148129.5
29	175427.5
30	202723.5
31	229970.5
32	260833.0
33	291206.5
34	320630.5
35	347829.5
36	374978.0
37	398244.5
38	411349.0
39	421739.0
40	432843.5
41	440401.0
42	444717.0
43	448647.0
44	450188.5
45	449767.5
46	447805.0
47	444911.0
48	437813.0
49	425784.5
50	411573.0
51	390511.5
52	363546.5
53	336543.0
54	310529.0
55	283304.5
56	254614.0
57	225512.0
58	197790.5
59	171518.5
60	145040.0
61	118321.0
62	91603.0
63	69460.0
64	52228.5
65	38275.0
66	28107.0
67	20902.0
68	15428.0
69	10365.5
70	6797.5
71	4727.5
72	3142.5
73	2082.5
74	1408.0
75	955.5
76	665.0
77	432.5
78	268.0
79	170.0
80	113.5
81	79.0
82	57.5
83	40.5
84	25.5
85	15.0
86	13.0
87	11.0
88	9.5
89	9.5
90	6.0
91	2.5
92	2.5
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	9.31408125157408E-5
10-11	0.0016020219752707417
12-13	1.7231050315412048E-4
14-15	0.0012248016845819915
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	156.0
20-21	592.0
22-23	1279.0
24-25	3754.0
26-27	3967.0
28-29	9201.0
30-31	22865.0
32-33	29248.0
34-35	41421.0
36-37	59886.0
38-39	70468.0
40-41	78003.0
42-43	94807.0
44-45	115774.0
46-47	111827.0
48-49	100016.0
50-51	85091.0
52-53	79110.0
54-55	84798.0
56-57	91707.0
58-59	93342.0
60-61	94639.0
62-63	92281.0
64-65	96478.0
66-67	104359.0
68-69	103101.0
70-71	100734.0
72-73	96277.0
74-75	97568.0
76-77	97411.0
78-79	104613.0
80-81	106911.0
82-83	102491.0
84-85	101332.0
86-87	107256.0
88-89	114783.0
90-91	119893.0
92-93	123595.0
94-95	132855.0
96-97	339146.0
98-99	619847.0
100-101	6703550.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.98369951122187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.18825209850586	75.79547616270524
2	7.729591877620545	13.137786269441712
3	1.7654917891497672	4.501140710959
4	0.6485232221173332	2.2045561053787535
5	0.2706948487336137	1.1502324842006542
6	0.1443380114195138	0.7359826914313962
7	0.07609646016923005	0.45268710934226575
8	0.04953091128945895	0.33674560652322927
9	0.031675418988844906	0.24227048603160417
>10	0.09367880314222633	1.2015471814962941
>50	0.001576900360301764	0.08911293336141404
>100	4.6429885637557953E-4	0.06831412658318418
>500	5.12161569040442E-5	0.03316631599643572
>1k	3.41434900689278E-5	0.05098181654879369
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.31408125157408E-6	0.0
3	0.0	0.0	0.0	9.31408125157408E-6	0.0
4	0.0	3.725632500629632E-5	0.0	1.862816250314816E-5	0.0
5	0.0	3.725632500629632E-5	0.0	2.794224375472224E-5	0.0
6	0.0	3.725632500629632E-5	0.0	2.794224375472224E-5	0.0
7	0.0	3.725632500629632E-5	0.0	2.794224375472224E-5	0.0
8	1.862816250314816E-5	3.725632500629632E-5	0.0	2.794224375472224E-5	0.0
9	1.862816250314816E-5	1.0245489376731488E-4	0.0	3.725632500629632E-5	0.0
10-11	1.862816250314816E-5	1.0245489376731488E-4	0.0	6.054152813523152E-5	0.0
12-13	1.862816250314816E-5	1.0245489376731488E-4	0.0	1.0245489376731488E-4	0.0
14-15	1.862816250314816E-5	1.2108305627046304E-4	0.0	1.397112187736112E-4	9.31408125157408E-6
16-17	1.862816250314816E-5	1.2108305627046304E-4	0.0	1.629964219025464E-4	1.862816250314816E-5
18-19	1.862816250314816E-5	1.397112187736112E-4	0.0	2.2819499066356497E-4	2.794224375472224E-5
20-21	2.7942243754722242E-5	1.4902530002518528E-4	0.0	3.120217219277317E-4	2.794224375472224E-5
22-23	4.1913365632083356E-5	1.6765346252833343E-4	0.0	5.169315094623614E-4	3.259928438050928E-5
24-25	4.65704062578704E-5	1.9559570628305568E-4	0.0	0.0017836465596764363	3.725632500629632E-5
26-27	4.65704062578704E-5	2.1422386878620382E-4	0.0	0.004447473797626623	3.725632500629632E-5
28-29	5.588448750944448E-5	2.1888090941199088E-4	0.0	0.012839461005294869	3.725632500629632E-5
30-31	6.054152813523151E-5	2.607942750440742E-4	0.0	0.015489317121367693	4.65704062578704E-5
32-33	6.519856876101856E-5	2.654513156698613E-4	0.0	0.02043509426595353	6.519856876101856E-5
34-35	6.519856876101856E-5	3.120217219277317E-4	0.0	0.027117947563957932	8.382673126416671E-5
36-37	6.519856876101856E-5	3.53935087559815E-4	0.0	0.03700018777187803	8.382673126416671E-5
38-39	6.519856876101856E-5	3.53935087559815E-4	0.0	0.06176632981981351	8.382673126416671E-5
40-41	9.31408125157408E-5	3.8187733131453725E-4	0.0	0.08831146138679963	9.31408125157408E-5
42-43	9.31408125157408E-5	3.8187733131453725E-4	0.0	0.10476478591770524	9.31408125157408E-5
44-45	1.0711193439310191E-4	4.005054938176854E-4	0.0	0.1260986890244357	9.31408125157408E-5
46-47	1.16426015644676E-4	4.191336563208336E-4	0.0	0.14580262791214066	9.779785314152784E-5
48-49	1.2108305627046304E-4	4.2844773757240765E-4	0.0	0.16500826345288638	1.2108305627046304E-4
50-51	1.629964219025464E-4	5.262455907139355E-4	0.0	0.18278418752151554	1.2108305627046304E-4
52-53	1.6765346252833343E-4	5.309026313397226E-4	0.0	0.22722632621340125	1.2108305627046304E-4
54-55	1.7231050315412048E-4	5.402167125912966E-4	0.0	0.2809872031974868	1.2108305627046304E-4
56-57	1.769675437799075E-4	5.588448750944448E-4	0.0	0.33494833292848125	1.2108305627046304E-4
58-59	1.769675437799075E-4	5.635019157202318E-4	0.0	0.37042566841572694	1.2108305627046304E-4
60-61	1.769675437799075E-4	6.007582407265282E-4	0.0	0.39413466224160876	1.2108305627046304E-4
62-63	1.8162458440569454E-4	6.240434438554634E-4	0.0	0.40849697553153597	1.2108305627046304E-4
64-65	2.0490978753462977E-4	7.264983376227782E-4	0.0	0.4140854242824804	1.2108305627046304E-4
66-67	2.235379500377779E-4	7.451265001259264E-4	0.0	0.4197530427240632	1.2108305627046304E-4
68-69	2.32852031289352E-4	7.637546626290746E-4	0.0	0.4255743435062971	1.2108305627046304E-4
70-71	2.5148019379250017E-4	7.870398657580098E-4	0.0	0.4307436586009207	1.2108305627046304E-4
72-73	2.5148019379250017E-4	8.010109876353708E-4	0.0	0.4347440564984717	1.397112187736112E-4
74-75	2.5148019379250017E-4	8.24296190764306E-4	0.0	0.43791084412400694	1.397112187736112E-4
76-77	2.5148019379250017E-4	8.941518001511117E-4	0.0	0.4412173429683157	1.397112187736112E-4
78-79	2.5148019379250017E-4	9.127799626542599E-4	0.0	0.44328972604679096	1.397112187736112E-4
80-81	2.561372344182872E-4	9.127799626542599E-4	0.0	0.44408607999380056	1.397112187736112E-4
82-83	2.607942750440742E-4	9.127799626542599E-4	0.0	0.4444865854876182	1.397112187736112E-4
84-85	2.607942750440742E-4	9.174370032800469E-4	0.0	0.4447147804782818	1.397112187736112E-4
86-87	2.607942750440742E-4	9.31408125157408E-4	0.0	0.4448312064939265	1.397112187736112E-4
88	2.607942750440742E-4	9.872926126668524E-4	0.0	0.44484983465642963	1.4902530002518528E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19445	0.0	54.10232	1
GTATCAA	36590	0.0	39.162796	1
ATCAACG	46035	0.0	30.689346	3
TCAACGC	46090	0.0	30.652727	4
TATCAAC	46685	0.0	30.397158	2
CAACGCA	46940	0.0	30.10802	5
AACGCAG	48385	0.0	29.208857	6
TGGTATC	7335	0.0	26.541006	2
GTGGTAT	7950	0.0	25.915852	1
ACGCAGA	55065	0.0	25.688404	7
CGCAGAG	55700	0.0	25.41855	8
GCAGAGT	63230	0.0	22.365294	9
GAGTACT	33620	0.0	20.611528	12-13
GTACATG	53590	0.0	20.533194	1
TACATGG	52730	0.0	20.485325	2
ACATGGG	53370	0.0	19.664547	3
CAGAGTA	62690	0.0	19.529425	10-11
AGAGTAC	60020	0.0	18.88488	10-11
GTACTTT	37470	0.0	18.46044	14-15
AGTACTT	36120	0.0	18.079	12-13
>>END_MODULE