FastQCFastQC Report
Fri 27 May 2016
SRR2139304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9522820
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA301000.060.90271
ATCTCGT130300.057.70811594
TGGTATC95600.039.642542
GTATCAA592150.038.6750641
TATCTCG158650.037.26134594
GTGGTAT108400.035.5610541
TGCGTAT19650.029.88804494
ACCGAAC25850.029.542758
TATCAAC777900.029.1894382
TCAACGC784100.028.912164
CCGAACC26300.028.8835749
ATCAACG789200.028.7201773
CAACGCA797950.028.4208135
GACCGAA26900.028.087147
GCCGTCT77000.027.90855690-91
AACGCAG821800.027.6207076
TTAGCCG15350.027.59780794
CCGTCTT76100.027.16678890-91
TGCCGTC74550.026.994688-89
AGGACCG31850.026.6551655
Produced by FastQC (version 0.11.5)