##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139304_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9522820
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62560722559074	33.0	33.0	33.0	33.0	33.0
2	32.612203947990196	33.0	33.0	33.0	33.0	33.0
3	32.585187370967844	33.0	33.0	33.0	33.0	33.0
4	36.50786836252286	37.0	37.0	37.0	37.0	37.0
5	36.539173690146406	37.0	37.0	37.0	37.0	37.0
6	36.54547224456621	37.0	37.0	37.0	37.0	37.0
7	36.50878038228172	37.0	37.0	37.0	37.0	37.0
8	36.517823291840024	37.0	37.0	37.0	37.0	37.0
9	36.53387063915941	37.0	37.0	37.0	37.0	37.0
10-11	36.47182615023701	37.0	37.0	37.0	37.0	37.0
12-13	36.458168063661816	37.0	37.0	37.0	37.0	37.0
14-15	38.37580380601544	40.0	37.0	40.0	37.0	40.0
16-17	38.312005792401834	40.0	37.0	40.0	37.0	40.0
18-19	38.412118521614396	40.0	37.0	40.0	37.0	40.0
20-21	38.445054525573894	40.0	37.0	40.0	37.0	40.0
22-23	38.448268332980554	40.0	37.0	40.0	37.0	40.0
24-25	38.417414496059635	40.0	37.0	40.0	37.0	40.0
26-27	38.27764128636292	40.0	37.0	40.0	37.0	40.0
28-29	38.2238794052635	40.0	37.0	40.0	37.0	40.0
30-31	38.12289424010076	40.0	37.0	40.0	37.0	40.0
32-33	38.025397107553616	40.0	37.0	40.0	33.0	40.0
34-35	37.98242560867394	40.0	37.0	40.0	33.0	40.0
36-37	37.933347612352826	40.0	37.0	40.0	33.0	40.0
38-39	37.87383278050764	40.0	37.0	40.0	33.0	40.0
40-41	37.83602058273497	40.0	37.0	40.0	33.0	40.0
42-43	37.77975787558904	40.0	37.0	40.0	33.0	40.0
44-45	37.74111224496299	40.0	37.0	40.0	33.0	40.0
46-47	37.687909526228935	40.0	37.0	40.0	33.0	40.0
48-49	37.6831889648995	40.0	37.0	40.0	33.0	40.0
50-51	37.63432171469226	40.0	37.0	40.0	33.0	40.0
52-53	37.542613111316044	40.0	37.0	40.0	33.0	40.0
54-55	37.39277409418881	40.0	37.0	40.0	33.0	40.0
56-57	37.311047374594395	40.0	37.0	40.0	33.0	40.0
58-59	37.18553796658312	37.0	37.0	40.0	33.0	40.0
60-61	37.03737980969983	37.0	37.0	40.0	33.0	40.0
62-63	36.840751398713465	37.0	37.0	40.0	33.0	40.0
64-65	36.66517353998465	37.0	37.0	40.0	33.0	40.0
66-67	36.446755139113954	37.0	37.0	40.0	33.0	40.0
68-69	36.15335532601915	37.0	37.0	40.0	33.0	40.0
70-71	35.920733854452145	37.0	37.0	37.0	33.0	40.0
72-73	35.6097634673971	37.0	33.0	37.0	33.0	40.0
74-75	35.50202925830545	37.0	33.0	37.0	33.0	40.0
76-77	33.78892175247486	35.0	33.0	37.0	27.0	37.0
78-79	35.03969138347027	37.0	33.0	37.0	33.0	37.0
80-81	35.07940679246617	37.0	33.0	37.0	33.0	37.0
82-83	34.92877603096473	37.0	33.0	37.0	33.0	37.0
84-85	34.8158554267679	37.0	33.0	37.0	33.0	37.0
86-87	34.738170238103955	37.0	33.0	37.0	33.0	37.0
88-89	34.63641665507651	37.0	33.0	37.0	33.0	37.0
90-91	34.56330829012636	37.0	33.0	37.0	33.0	37.0
92-93	34.56329175158131	37.0	33.0	37.0	33.0	37.0
94-95	34.534018186464664	37.0	33.0	37.0	33.0	37.0
96-97	34.51869190199929	37.0	33.0	37.0	33.0	37.0
98-99	34.5436751936986	37.0	33.0	37.0	33.0	37.0
100	34.44149200476541	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	2.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	5.0
18	9.0
19	43.0
20	127.0
21	488.0
22	1599.0
23	4259.0
24	9231.0
25	17684.0
26	30522.0
27	48561.0
28	71966.0
29	101322.0
30	138269.0
31	184168.0
32	243427.0
33	327802.0
34	455259.0
35	663833.0
36	1168607.0
37	2892882.0
38	3059821.0
39	102929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65538779479188	20.297359395641205	14.703155157820897	21.344097651746015
2	15.32367512984599	21.51564347535709	41.90612654654819	21.254554848248734
3	18.11561071195297	28.70269521003232	30.33664397730924	22.845050100705464
4	12.070888665332328	18.410722874106618	39.188822218628516	30.329566241932536
5	12.197647335558164	39.15909362982814	34.75549259568069	13.887766438933005
6	27.72142075561651	38.655849842798666	18.999424540209727	14.623304861375097
7	26.41914894957586	33.964760438609574	22.728088948441744	16.888001663372826
8	25.527805839026673	35.133227342320865	20.800687191399188	18.53827962725327
9	27.74023863984571	15.82805144956285	21.064503913869046	35.3672059967224
10-11	24.57646866341716	27.327771028971696	27.590921959718866	20.504838347892274
12-13	26.13892423679501	25.964248757876096	27.83402931196091	20.06279769336798
14-15	23.44411299037761	26.140158759812227	27.239785367302318	23.175942882507847
16-17	20.695497762217492	29.42560607047072	27.906134947421034	21.972761219890746
18-19	20.615489949405745	27.490381000585963	31.46544826007422	20.428680789934074
20-21	22.47480041957526	26.270856197736347	31.48896355431237	19.76537982837602
22-23	22.084673995905515	26.330119355021793	31.328711517958695	20.256495131114
24-25	21.27455740087215	27.051854195750092	31.534368221365238	20.139220182012515
26-27	20.803829423165123	27.16654000466977	31.61433326359274	20.415297308572363
28-29	20.509231297229512	26.774359851105658	31.946407461116422	20.770001390548412
30-31	21.08807507655967	26.955401158276132	31.508001009922577	20.448522755241623
32-33	20.746794858836324	27.43733618929894	31.151409019400976	20.66445993246376
34-35	20.971309310836904	27.334982331701944	31.352228575731317	20.34147978172983
36-37	21.150434732259598	27.166811420322873	30.895433998392225	20.787319849025305
38-39	20.700619218966526	27.711464495993273	30.73193172786074	20.855984557179465
40-41	21.01926754265066	27.25625172075204	31.124313131715798	20.6001676048815
42-43	20.951790262877882	27.435033107308243	30.177709415707575	21.435467214106293
44-45	20.884067590658134	27.86729473290559	29.808852660065828	21.439785016370454
46-47	21.37522781543913	27.810457324805775	29.21455545722195	21.599759402533145
48-49	21.222316159517252	28.583419424128092	28.406264110920503	21.788000305434153
50-51	21.467134698072833	28.641146267503835	27.527420426644543	22.364298607778785
52-53	21.40465934277932	28.582818380315018	27.469034583739422	22.543487693166245
54-55	21.878288267668484	28.255198895361623	27.822258126511894	22.044254710458002
56-57	21.43901799564097	28.113731162651952	27.620579443318643	22.826671398388438
58-59	21.038621049196493	27.705915349407164	28.02207818899062	23.233385412405728
60-61	21.751268730157054	28.59407786878863	27.813824014654138	21.84082938640018
62-63	20.879052237598138	28.509776726599533	28.266501927193328	22.344669108609
64-65	20.688557870616535	28.65979411126084	27.798653934877947	22.852994083244678
66-67	20.846676510661123	29.549209299848535	27.07445699000744	22.529657199482905
68-69	20.994456098937313	29.60408706552314	26.974429287567343	22.42702754797221
70-71	21.16783477332032	28.828302350938344	27.065369384230202	22.938493491511135
72-73	21.50820090139814	28.215452505548182	26.685441491931357	23.59090510112232
74-75	21.303137646048036	28.409219185034285	27.033733174787315	23.25390999413036
76-77	21.734667883407358	27.982182714135522	26.724602012303304	23.558547390153816
78-79	22.21207115158838	27.758094594878173	26.001290562711848	24.028543690821596
80-81	21.769385092186656	28.78274029703856	26.086393901111897	23.361480709662885
82-83	22.05226917517497	28.10460050801057	26.26610949071919	23.577020826095268
84-85	21.853171114321256	28.594825405110168	26.08132354266574	23.470679937902833
86-87	22.455707318580014	28.61217122948643	26.614192339346403	22.317929112587144
88-89	22.35391073061818	28.51336838882956	26.915782220540592	22.216938660011675
90-91	22.463279295459607	27.8867550195396	26.75127580821495	22.89868987678585
92-93	22.3493356972854	27.413001436284045	27.006269015862145	23.231393850568406
94-95	21.310883581915142	27.48109757753372	28.078727878032023	23.129290962519118
96-97	21.71000139210125	27.42753229257347	28.088306081958198	22.774160233367084
98-99	22.701434282074032	29.13802167788731	28.659633175113196	19.50091086492546
100	29.64887021039251	40.265380324918034	30.08574946468946	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9090.0
1	7160.0
2	6641.0
3	8654.0
4	12636.0
5	21864.5
6	29104.0
7	31628.5
8	31126.0
9	27871.5
10	23826.0
11	21812.0
12	25233.0
13	39106.5
14	69035.0
15	110670.0
16	146595.5
17	165761.5
18	168955.5
19	152495.0
20	126981.0
21	100726.5
22	76699.0
23	62365.0
24	56155.0
25	58130.5
26	66139.5
27	76153.0
28	87683.0
29	105438.0
30	131093.5
31	155871.0
32	198087.0
33	256568.5
34	291045.5
35	325229.5
36	376239.0
37	404004.0
38	421238.5
39	420522.0
40	409751.5
41	393620.5
42	371784.0
43	378775.0
44	386754.5
45	380439.0
46	362038.5
47	347662.5
48	349112.5
49	346239.5
50	336905.5
51	327781.5
52	317227.0
53	305745.5
54	290072.0
55	270632.0
56	248849.5
57	224295.5
58	200657.5
59	175905.5
60	151789.5
61	126681.5
62	98382.5
63	75135.5
64	57573.5
65	45258.5
66	36218.5
67	28224.5
68	21918.5
69	14157.0
70	7668.5
71	5495.0
72	3941.0
73	2715.5
74	1850.5
75	1283.5
76	868.0
77	564.5
78	389.5
79	287.5
80	208.0
81	156.0
82	123.5
83	90.5
84	61.5
85	42.5
86	30.0
87	27.5
88	24.0
89	18.0
90	13.0
91	7.5
92	4.0
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.890196391405067E-4
10-11	0.0015541614773774994
12-13	1.890196391405067E-4
14-15	0.0013966451114270772
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4244.0
20-21	10946.0
22-23	8170.0
24-25	17386.0
26-27	14555.0
28-29	16011.0
30-31	41924.0
32-33	46127.0
34-35	61733.0
36-37	82984.0
38-39	72259.0
40-41	112881.0
42-43	133260.0
44-45	160422.0
46-47	148740.0
48-49	126805.0
50-51	123159.0
52-53	23747.0
54-55	20475.0
56-57	23094.0
58-59	60821.0
60-61	104268.0
62-63	95955.0
64-65	99565.0
66-67	108122.0
68-69	113851.0
70-71	111704.0
72-73	103821.0
74-75	102497.0
76-77	102332.0
78-79	113057.0
80-81	115358.0
82-83	109158.0
84-85	108483.0
86-87	111288.0
88-89	116585.0
90-91	123185.0
92-93	125953.0
94-95	139771.0
96-97	279787.0
98-99	1919449.0
100-101	4008888.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.63546569093162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.88196731336583	74.33684727779514
2	7.830222000475868	13.097685269463549
3	1.8232154506230418	4.574564198032795
4	0.6885163973758079	2.303375581214729
5	0.3097267475119567	1.295207038255005
6	0.15658916850686716	0.7857844818136559
7	0.09381416439271226	0.5492333929173073
8	0.05504436150498812	0.3682928646503737
9	0.035854626369844406	0.2698846536254665
>10	0.11956929305997123	1.5216661370571871
>50	0.0035350158911492133	0.19792269655689604
>100	0.001551586359806493	0.24842525064042387
>500	1.865736981115836E-4	0.11127481571795389
>1k	2.0730086420448404E-4	0.339836342259557
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0501091063361484E-5	0.0
2	0.0	0.0	0.0	1.0501091063361484E-5	0.0
3	0.0	0.0	0.0	2.1002182126722967E-5	0.0
4	0.0	0.0	0.0	3.150327319008445E-5	0.0
5	0.0	0.0	0.0	3.150327319008445E-5	0.0
6	0.0	0.0	0.0	3.150327319008445E-5	0.0
7	0.0	0.0	0.0	3.150327319008445E-5	0.0
8	0.0	0.0	0.0	3.150327319008445E-5	0.0
9	0.0	0.0	0.0	8.400872850689187E-5	0.0
10-11	0.0	5.250545531680742E-6	0.0	1.522658204187415E-4	0.0
12-13	0.0	1.0501091063361484E-5	0.0	2.6252727658403706E-4	0.0
14-15	0.0	3.150327319008445E-5	0.0	2.887800042424408E-4	0.0
16-17	0.0	3.150327319008445E-5	0.0	4.2529418806614013E-4	0.0
18-19	0.0	3.6753818721765193E-5	0.0	0.0018639436637466634	0.0
20-21	0.0	4.2004364253445934E-5	0.0	0.0030978218636916375	0.0
22-23	0.0	4.2004364253445934E-5	0.0	0.004567974612562245	0.0
24-25	1.0501091063361484E-5	4.7254909785126675E-5	0.0	0.008831417584287009	0.0
26-27	1.0501091063361484E-5	5.2505455316807416E-5	0.0	0.018224643540463853	0.0
28-29	1.0501091063361484E-5	5.2505455316807416E-5	0.0	0.052988505505722046	0.0
30-31	1.0501091063361484E-5	5.2505455316807416E-5	0.0	0.12030574976740083	0.0
32-33	1.0501091063361484E-5	5.2505455316807416E-5	0.0	0.1354430725352364	0.0
34-35	1.0501091063361484E-5	7.350763744353039E-5	0.0	0.15284863097275808	0.0
36-37	1.0501091063361484E-5	1.1026145616529558E-4	0.0	0.18380059688201605	0.0
38-39	1.0501091063361484E-5	1.3126363829201853E-4	0.0	0.41250910969649746	0.0
40-41	1.0501091063361484E-5	1.4701527488706077E-4	0.0	0.6544647488874094	0.0
42-43	1.0501091063361484E-5	1.4701527488706077E-4	0.0	0.7102360435249222	0.0
44-45	1.0501091063361484E-5	1.4701527488706077E-4	0.0	0.7816067089370585	0.0
46-47	1.0501091063361484E-5	1.5751636595042224E-4	0.0	0.8553873747482363	0.0
48-49	1.0501091063361484E-5	1.5751636595042224E-4	0.0	0.9278501536309622	0.0
50-51	1.0501091063361484E-5	1.6801745701378374E-4	0.0	0.9977769190218864	0.0
52-53	1.0501091063361484E-5	1.6801745701378374E-4	0.0	1.6329879174446225	0.0
54-55	1.0501091063361484E-5	1.785185480771452E-4	0.0	2.4012844934588706	0.0
56-57	1.0501091063361484E-5	1.890196391405067E-4	0.0	3.2338634984174854	0.0
58-59	1.0501091063361484E-5	1.9427018467218745E-4	0.0	4.1334604665424735	0.0
60-61	1.0501091063361484E-5	1.9952073020386817E-4	0.0	4.394664605652527	0.0
62-63	1.0501091063361484E-5	2.1002182126722967E-4	0.0	4.405092189078445	0.0
64-65	1.0501091063361484E-5	2.1002182126722967E-4	0.0	4.4134615586559445	0.0
66-67	1.0501091063361484E-5	2.2052291233059116E-4	0.0	4.419420927834402	0.0
68-69	1.0501091063361484E-5	2.2052291233059116E-4	0.0	4.425616571561785	0.0
70-71	1.0501091063361484E-5	2.2577345786227188E-4	0.0	4.431770210924915	0.0
72-73	1.0501091063361484E-5	2.3627454892563338E-4	0.0	4.436763479725544	0.0
74-75	1.0501091063361484E-5	2.4152509445731412E-4	0.0	4.440381105596872	0.0
76-77	1.0501091063361484E-5	2.4152509445731412E-4	0.0	4.44387271837544	0.0
78-79	1.0501091063361484E-5	2.4152509445731412E-4	0.0	4.446697511871484	0.0
80-81	1.0501091063361484E-5	2.572767310523563E-4	0.0	4.447563851884211	0.0
82-83	1.0501091063361484E-5	2.7302836764739856E-4	0.0	4.448199167893544	0.0
84-85	1.0501091063361484E-5	2.7302836764739856E-4	0.0	4.448487947897787	0.0
86-87	1.0501091063361484E-5	2.7302836764739856E-4	0.0	4.44856145553523	0.0
88	1.0501091063361484E-5	2.7302836764739856E-4	0.0	4.448566706080761	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30100	0.0	60.9027	1
ATCTCGT	13030	0.0	57.708115	94
TGGTATC	9560	0.0	39.64254	2
GTATCAA	59215	0.0	38.675064	1
TATCTCG	15865	0.0	37.261345	94
GTGGTAT	10840	0.0	35.561054	1
TGCGTAT	1965	0.0	29.888044	94
ACCGAAC	2585	0.0	29.54275	8
TATCAAC	77790	0.0	29.189438	2
TCAACGC	78410	0.0	28.91216	4
CCGAACC	2630	0.0	28.883574	9
ATCAACG	78920	0.0	28.720177	3
CAACGCA	79795	0.0	28.420813	5
GACCGAA	2690	0.0	28.08714	7
GCCGTCT	7700	0.0	27.908556	90-91
AACGCAG	82180	0.0	27.620707	6
TTAGCCG	1535	0.0	27.597807	94
CCGTCTT	7610	0.0	27.166788	90-91
TGCCGTC	7455	0.0	26.9946	88-89
AGGACCG	3185	0.0	26.655165	5
>>END_MODULE