FastQCFastQC Report
Fri 27 May 2016
SRR2139303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17479933
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA265750.049.8422241
GTATCAA526150.035.9647561
ATCAACG694000.030.9355053
TCAACGC703500.030.5543254
CAACGCA712950.030.1373065
TATCAAC728200.029.7485262
AACGCAG734050.029.3230066
ACGCAGA820800.026.2029747
CGCAGAG832900.025.8171628
GCAGAGT959750.022.3915089
TACATGG824900.021.8947432
ACATGGG824450.021.3235073
GTACATG808850.020.1715561
AGAGTAC904800.019.86311110-11
TGGTATC113300.019.6422042
GAGTACT507000.019.52748912-13
CAGAGTA956800.019.1622710-11
CATGGGG452250.018.7442134
TTATCAA165100.018.6738821
AGTACTT549200.017.99188212-13
Produced by FastQC (version 0.11.5)