##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17479933
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.636691227592234	18.0	18.0	30.0	18.0	33.0
2	30.390983592442833	32.0	28.0	33.0	27.0	33.0
3	32.019595670074935	33.0	32.0	33.0	30.0	33.0
4	32.3786040827502	33.0	33.0	33.0	31.0	34.0
5	32.8427297175567	33.0	33.0	34.0	32.0	34.0
6	36.726067828749684	38.0	37.0	38.0	34.0	38.0
7	36.98779817977563	38.0	38.0	38.0	35.0	38.0
8	37.04645750072383	38.0	38.0	38.0	36.0	38.0
9	37.17654930370728	38.0	38.0	38.0	36.0	38.0
10-11	37.20601346126441	38.0	38.0	38.0	36.0	38.0
12-13	37.22186538129179	38.0	38.0	38.0	36.0	38.0
14-15	37.213180107726956	38.0	38.0	38.0	36.0	38.0
16-17	37.169850708237846	38.0	38.0	38.0	36.0	38.0
18-19	37.19374124603338	38.0	38.0	38.0	36.0	38.0
20-21	37.209613799147576	38.0	38.0	38.0	36.0	38.0
22-23	37.21200642951875	38.0	38.0	38.0	36.0	38.0
24-25	37.21448112348464	38.0	38.0	38.0	36.0	38.0
26-27	37.18209212753872	38.0	38.0	38.0	36.0	38.0
28-29	37.15254010772529	38.0	38.0	38.0	36.0	38.0
30-31	37.15208007720412	38.0	38.0	38.0	36.0	38.0
32-33	37.14079628257949	38.0	38.0	38.0	36.0	38.0
34-35	37.11461802463264	38.0	38.0	38.0	36.0	38.0
36-37	37.09383207977804	38.0	38.0	38.0	36.0	38.0
38-39	37.07105338203929	38.0	38.0	38.0	36.0	38.0
40-41	37.05216570408119	38.0	38.0	38.0	36.0	38.0
42-43	37.02143388334075	38.0	38.0	38.0	36.0	38.0
44-45	36.96120997384621	38.0	38.0	38.0	36.0	38.0
46-47	36.922301459951356	38.0	38.0	38.0	36.0	38.0
48-49	36.881286538934944	38.0	38.0	38.0	36.0	38.0
50-51	36.83652795207358	38.0	38.0	38.0	35.0	38.0
52-53	36.81050303176794	38.0	38.0	38.0	35.0	38.0
54-55	36.78155418850933	38.0	38.0	38.0	35.0	38.0
56-57	36.780426801046886	38.0	38.0	38.0	35.0	38.0
58-59	36.763988882524785	38.0	38.0	38.0	35.0	38.0
60-61	36.77359221778436	38.0	38.0	38.0	35.0	38.0
62-63	36.7599733673618	38.0	38.0	38.0	35.0	38.0
64-65	36.75218157235002	38.0	38.0	38.0	35.0	38.0
66-67	36.74222033451974	38.0	38.0	38.0	35.0	38.0
68-69	36.74931372980552	38.0	38.0	38.0	35.0	38.0
70-71	36.72722524170543	38.0	38.0	38.0	35.0	38.0
72-73	36.70939021972667	38.0	38.0	38.0	34.5	38.0
74-75	36.72337945935179	38.0	38.0	38.0	35.0	38.0
76-77	36.69914474789363	38.0	38.0	38.0	34.5	38.0
78-79	36.67334731346146	38.0	38.0	38.0	34.0	38.0
80-81	36.66273505454101	38.0	38.0	38.0	34.0	38.0
82-83	36.60251973776137	38.0	38.0	38.0	34.0	38.0
84-85	36.59687190551332	38.0	38.0	38.0	34.0	38.0
86-87	36.583069644111646	38.0	38.0	38.0	34.0	38.0
88-89	36.55506649387226	38.0	38.0	38.0	34.0	38.0
90-91	36.52998152284337	38.0	38.0	38.0	34.0	38.0
92-93	36.53112698454724	38.0	38.0	38.0	34.0	38.0
94-95	36.47146674196932	38.0	38.0	38.0	34.0	38.0
96-97	36.43396707087936	38.0	38.0	38.0	34.0	38.0
98-99	36.455298473666446	38.0	38.0	38.0	34.0	38.0
100	34.70045435882488	38.0	34.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	8.0
18	57.0
19	107.0
20	269.0
21	661.0
22	2114.0
23	5838.0
24	13288.0
25	25683.0
26	44620.0
27	70762.0
28	106567.0
29	152406.0
30	205035.0
31	266105.0
32	342277.0
33	451544.0
34	651722.0
35	1105143.0
36	3076782.0
37	1.0958945E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.33041385227278	18.61662742071151	22.667220749644752	22.385737977370965
2	13.474752206417268	22.641358421205954	42.63263618778329	21.25125318459349
3	17.16222253254632	28.44733443772353	31.608313372825858	22.78212965690429
4	10.957690741720807	19.842169875593918	41.29726355358456	27.902875829100722
5	11.733341129118395	38.84933182703815	37.00455120906848	12.412775834774973
6	26.451158040263227	40.80694538687508	20.340071369895938	12.401825202965759
7	24.261208552687243	34.040250611944565	24.9247007983383	16.773840037029892
8	23.379992360382616	37.35935944376904	22.421418892166233	16.839229303682114
9	25.695521830661477	16.3854232164391	21.902658322546202	36.01639663035321
10-11	22.888594595871734	27.708993507011726	30.2529792305268	19.149432666589743
12-13	24.329235129219317	25.571402361782507	30.20810777707214	19.89125473192603
14-15	21.3937919556099	26.512910547197176	28.264284537017392	23.829012960175532
16-17	20.309951988946413	29.756412681902155	28.713656968822477	21.219978360328955
18-19	20.356662694302088	28.692773021498425	30.866333984232092	20.0842302999674
20-21	21.740446132256114	27.670348870138113	30.550233787215593	20.038971210390187
22-23	21.26518027552059	27.736777855115612	30.413517174687172	20.58452469467663
24-25	20.72282933346021	27.9362137851977	30.640326362341014	20.700630519001074
26-27	20.75795232418326	28.0363001451968	30.511928110714116	20.69381941990582
28-29	20.628083733544297	28.094215708688342	30.608761572350996	20.668938985416364
30-31	20.942605549532146	27.96560944196872	30.486124937607066	20.605660070892068
32-33	20.72624208662746	28.091640126108793	30.452623669049384	20.72949411821436
34-35	20.778548704734735	28.169439704107052	30.540577598164397	20.51143399299382
36-37	20.776236426264745	28.095289031453852	30.42756750132322	20.700907040958185
38-39	20.66243006823041	28.113902316698347	30.527510671400982	20.696156943670257
40-41	20.833558756340032	28.030050898933425	30.42147829823883	20.71491204648772
42-43	20.841381480069245	28.079947970057162	30.21001777364566	20.86865277622794
44-45	20.885347730307636	28.310024953293794	29.9700523176411	20.83457499875747
46-47	20.94343816689849	28.302998649207602	29.653384830551182	21.100178353342727
48-49	21.024790428718283	28.393566694439144	29.546702958925668	21.03493991791691
50-51	20.971036436599984	28.50202549489447	29.380217618409215	21.146720450096335
52-53	21.04006052492375	28.5220732787398	29.366683939845238	21.071182256491205
54-55	21.081787791504507	28.509578555876963	29.316187944465344	21.092445708153182
56-57	21.161364607032972	28.525181083286412	29.19332952078107	21.120124788899545
58-59	21.053522332376073	28.582810025721262	29.30645916612797	21.057208475774694
60-61	21.142316553830316	28.607767274781647	29.22566870787271	21.024247463515326
62-63	21.060591478858925	28.64240033492121	29.225522086717614	21.07148609950225
64-65	21.01540462540261	28.607345235396647	29.277745767339418	21.099504371861325
66-67	21.139310904665336	28.635001154810407	29.117714493980458	21.107973446543795
68-69	21.139037068761255	28.554415315786567	29.18865829165467	21.11788932379751
70-71	21.120435753439995	28.36357340291013	29.266631554753157	21.24935928889672
72-73	21.156725800804164	28.397160498689573	29.242061990050004	21.204051710456255
74-75	21.11135690823218	28.47230910747506	29.17560115696571	21.24073282732705
76-77	21.17612777473763	28.477270449213567	29.09370648599154	21.252895290057268
78-79	21.186547644902305	28.49871995383606	29.020873000914506	21.29385940034713
80-81	21.197617694418312	28.50959683504708	28.999715741591686	21.293069728942925
82-83	21.23698121928113	28.4975836078109	29.08612316415034	21.179312008757638
84-85	21.337019062599925	28.505890957754975	28.94696596009261	21.21012401955249
86-87	21.159255769513287	28.576744533885297	28.998093222195507	21.265906474405906
88-89	21.280587767245667	28.533364500334496	28.919586093344236	21.266461639075597
90-91	21.329083638010964	28.521436472846943	28.94300894043021	21.206470948711882
92-93	21.286256772610333	28.539322662540272	28.926673225935005	21.24774733891439
94-95	21.181098568935973	28.614470314942636	28.95594738396681	21.24848373215459
96-97	21.27814417317223	28.778680517178245	28.974342864117524	20.968832445531994
98-99	22.214946063088775	30.631094013575122	29.318238898071712	17.835721025264387
100	21.156385343617345	31.57108670367142	23.35189376795777	23.920634184753464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2901.0
1	4362.5
2	8409.5
3	11379.5
4	14017.5
5	18456.0
6	21528.5
7	27159.5
8	31459.0
9	30710.0
10	29414.5
11	28972.0
12	32454.0
13	41902.0
14	63069.5
15	88769.0
16	103934.5
17	105435.5
18	97046.5
19	85071.0
20	74443.5
21	68651.0
22	71369.5
23	84814.5
24	108222.5
25	140087.5
26	181074.5
27	228711.0
28	277690.0
29	327918.0
30	376803.0
31	423316.5
32	469552.0
33	512763.5
34	557078.0
35	598561.0
36	636243.0
37	672337.5
38	697453.5
39	714533.0
40	729192.0
41	737357.0
42	739058.5
43	744784.0
44	747683.5
45	742976.0
46	731339.5
47	712282.0
48	691196.5
49	661199.5
50	625223.5
51	586649.5
52	540969.0
53	491078.0
54	441356.0
55	394044.5
56	349244.5
57	304901.0
58	262231.5
59	221873.5
60	181971.0
61	142702.0
62	108133.5
63	81526.5
64	60399.0
65	43866.5
66	31434.5
67	22604.5
68	16239.5
69	11312.5
70	7916.5
71	5456.0
72	3744.5
73	2578.5
74	1683.5
75	1031.0
76	638.5
77	386.0
78	234.5
79	141.0
80	94.0
81	65.5
82	50.5
83	34.5
84	20.0
85	14.0
86	10.5
87	8.0
88	5.5
89	2.5
90	2.0
91	1.5
92	2.0
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0026601932627544965
3	0.0
4	0.0
5	0.0022997799819942104
6	8.00918401689526E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.4303571681948982E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	2.583254785644532E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	2.919671884937833E-6
42-43	0.0
44-45	0.0
46-47	1.4934461757368581E-5
48-49	3.797003513313108E-4
50-51	0.0015196033543481307
52-53	0.0039838393916833414
54-55	0.005529105312125704
56-57	0.006387677995163882
58-59	0.007929120675861712
60-61	0.007408772852361443
62-63	0.005388104326692357
64-65	0.0032767145304477704
66-67	0.0010871730982123056
68-69	5.779109505178931E-4
70-71	1.945041500757099E-4
72-73	5.493304394383833E-4
74-75	1.678941801933691E-5
76-77	2.742898846529764E-4
78-79	2.1184852393174185E-4
80-81	7.590338920018287E-5
82-83	1.3938175827300427E-5
84-85	1.3011252553194572E-4
86-87	1.1707368575208986E-4
88-89	1.7903834779402197E-5
90-91	1.4470986847139168E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	193.0
20-21	1101.0
22-23	2134.0
24-25	1886.0
26-27	4503.0
28-29	12129.0
30-31	29582.0
32-33	37394.0
34-35	49984.0
36-37	80408.0
38-39	109118.0
40-41	105776.0
42-43	119271.0
44-45	147858.0
46-47	150728.0
48-49	142789.0
50-51	128431.0
52-53	113561.0
54-55	118577.0
56-57	126022.0
58-59	131493.0
60-61	134724.0
62-63	122511.0
64-65	123768.0
66-67	136286.0
68-69	144442.0
70-71	153169.0
72-73	131051.0
74-75	123971.0
76-77	129649.0
78-79	139263.0
80-81	144577.0
82-83	133920.0
84-85	124688.0
86-87	127409.0
88-89	139479.0
90-91	147964.0
92-93	146824.0
94-95	166122.0
96-97	508693.0
98-99	1175936.0
100-101	1.1712549E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.76609041660589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.50366985619546	73.25102741519311
2	7.764423821461876	12.852620080799246
3	1.9898510970226841	4.940765874352857
4	0.7726070699969673	2.557826664475118
5	0.3761722963484042	1.5567155145897145
6	0.20051423916090713	0.9957467788925147
7	0.1106469970345713	0.6410473552622477
8	0.07599662347224662	0.5031954727728575
9	0.05232538233260011	0.3897690592701108
>10	0.14937304729081247	1.8659514108356676
>50	0.002982523473201134	0.17381216642507463
>100	0.0013598718311247484	0.21084617521934407
>500	3.861246784090648E-5	0.02118507130821927
>1k	3.8561911320055205E-5	0.03949096060389212
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1441691452707514E-5
2	5.720845726353757E-6	0.0	0.0	0.0	1.1441691452707514E-5
3	5.720845726353757E-6	0.0	0.0	1.1441691452707514E-5	1.1441691452707514E-5
4	5.720845726353757E-6	1.716253717906127E-5	0.0	4.0045920084476294E-5	1.1441691452707514E-5
5	2.2883382905415028E-5	1.716253717906127E-5	0.0	4.0045920084476294E-5	1.1441691452707514E-5
6	2.2883382905415028E-5	1.716253717906127E-5	0.0	4.0045920084476294E-5	1.716253717906127E-5
7	2.2883382905415028E-5	1.716253717906127E-5	0.0	5.148761153718381E-5	1.716253717906127E-5
8	2.2883382905415028E-5	2.2883382905415028E-5	0.0	5.148761153718381E-5	1.716253717906127E-5
9	2.2883382905415028E-5	3.432507435812254E-5	0.0	1.315794517061364E-4	2.2883382905415028E-5
10-11	2.8604228631768783E-5	3.432507435812254E-5	0.0	1.5446283461155143E-4	2.2883382905415028E-5
12-13	2.8604228631768783E-5	3.432507435812254E-5	0.0	1.6304410320108206E-4	2.2883382905415028E-5
14-15	4.290634294765317E-5	4.0045920084476294E-5	0.0	1.945087546960277E-4	2.2883382905415028E-5
16-17	6.0068880126714444E-5	4.0045920084476294E-5	0.0	2.231129833277965E-4	2.2883382905415028E-5
18-19	6.292930298989132E-5	5.148761153718381E-5	0.0	2.374150976436809E-4	2.8604228631768783E-5
20-21	7.437099444259883E-5	6.292930298989132E-5	0.0	2.517172119595653E-4	2.8604228631768783E-5
22-23	8.295226303212947E-5	7.723141730577572E-5	0.0	2.7746101772815716E-4	4.0045920084476294E-5
24-25	1.0869606880072138E-4	9.725437734801387E-5	0.0	2.9462355490721847E-4	4.0045920084476294E-5
26-27	1.2585860597978264E-4	1.0297522307436762E-4	0.0	3.3466947499169474E-4	4.0045920084476294E-5
28-29	1.315794517061364E-4	1.0297522307436762E-4	0.0	4.6624892669783116E-4	4.0045920084476294E-5
30-31	1.315794517061364E-4	1.2013776025342889E-4	0.0	0.001058356459375445	4.0045920084476294E-5
32-33	1.3730029743249015E-4	1.2013776025342889E-4	0.0	0.0020795274215295906	4.5766765810830056E-5
34-35	1.430211431588439E-4	1.3730029743249015E-4	0.0	0.003363857287096009	4.5766765810830056E-5
36-37	1.4874198888519766E-4	1.4874198888519766E-4	0.0	0.005120156925086612	4.5766765810830056E-5
38-39	1.4874198888519766E-4	1.5446283461155143E-4	0.0	0.008278063766033886	4.862718867400693E-5
40-41	1.5446283461155143E-4	1.716253717906127E-4	0.0	0.012125332517006787	5.148761153718381E-5
42-43	1.573232574747283E-4	1.7734621751696645E-4	0.0	0.015569281644271748	5.148761153718381E-5
44-45	1.6304410320108206E-4	1.9164833183285085E-4	0.0	0.019399387858065588	5.148761153718381E-5
46-47	1.7162537179061268E-4	2.0022960042238147E-4	0.0	0.023827322450263394	5.148761153718381E-5
48-49	1.7734621751696645E-4	2.0022960042238147E-4	0.0	0.02845834706574676	5.7208457263537566E-5
50-51	1.7734621751696645E-4	2.3455467478050403E-4	0.0	0.03319806775003085	5.7208457263537566E-5
52-53	1.7734621751696645E-4	2.3455467478050403E-4	0.0	0.037777604753977026	6.865014871624508E-5
54-55	1.7734621751696645E-4	2.3455467478050403E-4	0.0	0.04215119131177448	8.009184016895259E-5
56-57	1.7734621751696645E-4	2.3455467478050403E-4	0.0	0.046295944040517775	8.009184016895259E-5
58-59	1.7734621751696645E-4	2.459963662332115E-4	0.0	0.050629484678230745	8.009184016895259E-5
60-61	1.8306706324332022E-4	2.517172119595653E-4	0.0	0.055071721384744435	8.581268589530636E-5
62-63	1.8306706324332022E-4	2.5743805768591905E-4	0.0	0.05956830612565849	8.581268589530636E-5
64-65	1.8306706324332022E-4	3.032048234967491E-4	0.0	0.06355859601979023	8.581268589530636E-5
66-67	1.8878790896967396E-4	3.117860920862798E-4	0.0	0.06767188409703859	8.581268589530636E-5
68-69	1.9450875469602773E-4	3.2036736067581035E-4	0.0	0.07174512625420246	8.581268589530636E-5
70-71	1.9736917755920462E-4	3.260882064021641E-4	0.0	0.07573255572547102	9.153353162166011E-5
72-73	2.0022960042238147E-4	3.318090521285179E-4	0.0	0.0789905773666295	1.0297522307436762E-4
74-75	2.0022960042238147E-4	3.461111664444023E-4	0.0	0.08139905341742443	1.0297522307436762E-4
76-77	2.0022960042238147E-4	3.7471539507617107E-4	0.0	0.08363876451929192	1.0297522307436762E-4
78-79	2.0595044614873524E-4	3.7757581793934793E-4	0.0	0.08496886115066918	1.0297522307436762E-4
80-81	2.0595044614873524E-4	3.7757581793934793E-4	0.0	0.0855352248775782	1.0297522307436762E-4
82-83	2.088108690119121E-4	3.7757581793934793E-4	0.0	0.08598431126709696	1.0869606880072138E-4
84-85	2.1739213760144275E-4	3.7757581793934793E-4	0.0	0.0862388889019197	1.0869606880072138E-4
86-87	2.1739213760144275E-4	3.8901750939205547E-4	0.0	0.08636474750789949	1.0869606880072138E-4
88	2.1739213760144275E-4	4.2906342947653174E-4	0.0	0.08637332877648901	1.0869606880072138E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	26575	0.0	49.842224	1
GTATCAA	52615	0.0	35.964756	1
ATCAACG	69400	0.0	30.935505	3
TCAACGC	70350	0.0	30.554325	4
CAACGCA	71295	0.0	30.137306	5
TATCAAC	72820	0.0	29.748526	2
AACGCAG	73405	0.0	29.323006	6
ACGCAGA	82080	0.0	26.202974	7
CGCAGAG	83290	0.0	25.817162	8
GCAGAGT	95975	0.0	22.391508	9
TACATGG	82490	0.0	21.894743	2
ACATGGG	82445	0.0	21.323507	3
GTACATG	80885	0.0	20.171556	1
AGAGTAC	90480	0.0	19.863111	10-11
TGGTATC	11330	0.0	19.642204	2
GAGTACT	50700	0.0	19.527489	12-13
CAGAGTA	95680	0.0	19.16227	10-11
CATGGGG	45225	0.0	18.744213	4
TTATCAA	16510	0.0	18.673882	1
AGTACTT	54920	0.0	17.991882	12-13
>>END_MODULE