FastQCFastQC Report
Fri 27 May 2016
SRR2139302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8159573
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203400.058.3333971
GTATCAA390550.038.8451581
TGGTATC66600.035.51872
GTGGTAT74000.033.2544631
ATCAACG473300.031.6680973
TCAACGC499850.029.9836254
TATCAAC505650.029.7407442
CAACGCA509650.029.4150355
AACGCAG515200.029.0970926
ACGCAGA518050.028.9290247
CGCAGAG601650.024.9368328
GCAGAGT667050.022.4671049
GAGTACT368400.021.45306812-13
TACATGG523550.021.2699432
GTACATG533650.021.0692231
ACATGGG516650.021.0565973
CAGAGTA663750.020.13313110-11
GTACTTT401500.019.58701514-15
AGAGTAC642000.018.5866210-11
CATGGGG306400.018.2380524
Produced by FastQC (version 0.11.5)