##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139302_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8159573
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85231494344128	33.0	33.0	33.0	33.0	33.0
2	32.90382499182249	33.0	33.0	33.0	33.0	33.0
3	33.57405810328555	33.0	33.0	33.0	33.0	37.0
4	36.719353696572114	37.0	37.0	37.0	37.0	37.0
5	36.746940434260466	37.0	37.0	37.0	37.0	37.0
6	36.75328059446248	37.0	37.0	37.0	37.0	37.0
7	36.74506092414395	37.0	37.0	37.0	37.0	37.0
8	36.723206349155774	37.0	37.0	37.0	37.0	37.0
9	36.727270654971775	37.0	37.0	37.0	37.0	37.0
10-11	36.703825249188895	37.0	37.0	37.0	37.0	37.0
12-13	36.96007603584158	37.0	37.0	37.0	37.0	38.5
14-15	38.899910081079	40.0	38.5	40.0	37.0	40.0
16-17	38.84384807881491	40.0	37.0	40.0	37.0	40.0
18-19	38.92041648502931	40.0	40.0	40.0	37.0	40.0
20-21	38.97835170854904	40.0	40.0	40.0	37.0	40.0
22-23	39.007875541803514	40.0	40.0	40.0	37.0	40.0
24-25	38.99571662389108	40.0	40.0	40.0	37.0	40.0
26-27	38.903049998713314	40.0	40.0	40.0	37.0	40.0
28-29	38.82947824255362	40.0	38.5	40.0	37.0	40.0
30-31	38.73021758046637	40.0	37.0	40.0	37.0	40.0
32-33	38.672439075123634	40.0	37.0	40.0	37.0	40.0
34-35	38.664133527666934	40.0	37.0	40.0	37.0	40.0
36-37	38.629282405296436	40.0	37.0	40.0	37.0	40.0
38-39	38.59841998715351	40.0	37.0	40.0	37.0	40.0
40-41	38.53931134855547	40.0	37.0	40.0	37.0	40.0
42-43	38.43466298795763	40.0	37.0	40.0	37.0	40.0
44-45	38.39071883575532	40.0	37.0	40.0	37.0	40.0
46-47	38.31762315981764	40.0	37.0	40.0	37.0	40.0
48-49	38.251801987169074	40.0	37.0	40.0	37.0	40.0
50-51	38.223755609519316	40.0	37.0	40.0	37.0	40.0
52-53	38.20217848233307	40.0	37.0	40.0	37.0	40.0
54-55	38.15147461485658	40.0	37.0	40.0	37.0	40.0
56-57	38.055104095426344	40.0	37.0	40.0	37.0	40.0
58-59	37.852961593094285	40.0	37.0	40.0	33.0	40.0
60-61	37.768147759469514	40.0	37.0	40.0	33.0	40.0
62-63	37.465327254075845	38.5	37.0	40.0	33.0	40.0
64-65	37.300983479812125	37.0	37.0	40.0	33.0	40.0
66-67	37.15042657862169	37.0	37.0	40.0	33.0	40.0
68-69	36.93083795497998	37.0	37.0	40.0	33.0	40.0
70-71	36.66531695198633	37.0	37.0	40.0	33.0	40.0
72-73	36.417681696520106	37.0	37.0	38.5	33.0	40.0
74-75	35.950046035046	37.0	35.0	37.0	33.0	40.0
76-77	34.6182253680403	37.0	33.0	37.0	30.0	37.0
78-79	35.77166396652388	37.0	37.0	37.0	33.0	37.0
80-81	35.86101960954057	37.0	37.0	37.0	33.0	37.0
82-83	35.74839195255623	37.0	37.0	37.0	33.0	37.0
84-85	35.3830154690789	37.0	37.0	37.0	33.0	37.0
86-87	35.45953031598643	37.0	33.0	37.0	33.0	37.0
88-89	35.45269420732897	37.0	33.0	37.0	33.0	37.0
90-91	35.39119630430047	37.0	33.0	37.0	33.0	37.0
92-93	35.32277762770626	37.0	33.0	37.0	33.0	37.0
94-95	35.28216066495809	37.0	33.0	37.0	33.0	37.0
96-97	35.24488062721061	37.0	33.0	37.0	33.0	37.0
98-99	35.20435220240425	37.0	33.0	37.0	33.0	37.0
100	35.12226091284365	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	0.0
17	3.0
18	4.0
19	9.0
20	41.0
21	152.0
22	526.0
23	1603.0
24	3626.0
25	7517.0
26	13982.0
27	22806.0
28	35165.0
29	51163.0
30	71409.0
31	97776.0
32	132873.0
33	180137.0
34	255636.0
35	388541.0
36	747727.0
37	2174302.0
38	3746810.0
39	227763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.48021997307693	0.0	24.98188624266258	28.537893784260493
2	15.369839768230698	24.512527922997176	39.28313928328635	20.834493025485777
3	16.354810010053882	25.913119403674724	33.24898514371243	24.48308544255897
4	12.449425034431737	24.294996779015978	37.80942439697724	25.446153789575042
5	16.856525723871872	36.70754147432172	31.99840036983175	14.437532431974661
6	26.06299373753994	38.064994332424014	21.11839675924218	14.753615170793863
7	25.56609273548996	33.90866164197563	22.41862411182546	18.106621510708955
8	23.64730360277431	32.07039142857108	23.067910538946094	21.214394429708516
9	24.93325324744322	20.469600553852512	23.300128572904487	31.297017625799782
10-11	24.417529227174587	26.71291908981751	28.194865601108393	20.674686081899512
12-13	25.305052765022477	25.20159145602245	28.393468431922653	21.099887347032418
14-15	22.561575533618676	27.063729568168526	27.669717235168704	22.70497766304409
16-17	20.305375123275475	29.60826616987584	28.56218176176174	21.524176945086943
18-19	20.414257022304604	27.97402853973223	31.291304249781724	20.32041018818144
20-21	22.115432873121005	26.80620740948365	31.24148863749383	19.836871079901513
22-23	21.6893130377756	26.832733858251697	30.944380548510047	20.533572555462655
24-25	20.883708875707146	27.166871650621033	31.337154960023224	20.612264513648597
26-27	20.736019182428016	27.387095954958003	31.171764514201257	20.705120348412727
28-29	20.583066478989874	27.515631253798915	31.257815473361354	20.643486793849853
30-31	20.867981059475444	27.345105501418722	31.23406848481463	20.552844954291206
32-33	20.67603389975386	27.540543489140656	31.06919508898966	20.71422752211582
34-35	20.86155718011567	27.574575022064273	30.97061022437904	20.59325757344101
36-37	20.765458975015818	27.601102245983377	30.891318334379697	20.742120444621104
38-39	20.652898657174983	27.781816557816143	30.871702823958213	20.69358196105066
40-41	20.990628407618843	27.70551492386799	30.592512241172802	20.71134442734036
42-43	20.9286499820371	27.742159309202574	30.38983453179689	20.939356176963436
44-45	21.087871883352715	28.09838363478809	29.850503889788456	20.963240592070743
46-47	21.157348094035836	28.171319309957365	29.330978192442487	21.34035440356431
48-49	21.248905022306555	28.27716548880363	29.120767161389537	21.35316232750028
50-51	21.269885751732506	28.551929679216652	28.795915201661558	21.382269367389284
52-53	21.419492206118505	28.489029035938536	28.705453478899635	21.386025279043324
54-55	21.486400557032656	28.436513040846844	28.64136819157831	21.435718210542188
56-57	21.477121197732558	28.60941758949424	28.472698288875314	21.44076292389789
58-59	21.4730614104521	28.545295868527447	28.53608573542143	21.44555698559902
60-61	21.50361491718751	28.544471079142248	28.516993366953812	21.43492063671643
62-63	21.45952340737339	28.59564329791842	28.449622396061162	21.495210898647024
64-65	21.41048137009379	28.48749667601937	28.511326200295084	21.590695753591756
66-67	21.54053016703088	28.539222147440764	28.328857378634282	21.59139030689408
68-69	21.60090432115075	28.574327063651445	28.34586628525752	21.478902329940286
70-71	21.518698899982276	28.24839317683992	28.479124529782563	21.753783393395242
72-73	21.516808705339983	28.325514045320716	28.448094953091807	21.709582296247497
74-75	21.460428544280465	28.4635415198276	28.336344915313262	21.739685020578676
76-77	21.580859850145735	28.389945809666767	28.244270634806877	21.784923705380624
78-79	21.559472969678954	28.388803546503887	28.31198371460073	21.739739769216428
80-81	21.52332642071496	28.430139163291962	28.271991532660113	21.774542883332966
82-83	21.74520586228721	28.286200558827606	28.337793116954224	21.630800461930956
84-85	21.807402573118875	28.189653327216284	28.248769556151736	21.75417454351311
86-87	21.611127072134792	28.337146950897306	28.303978899299654	21.747747077668247
88-89	21.76036432654657	28.19096489629508	28.283026403710398	21.76564437344795
90-91	21.769216711002155	28.137004963974725	28.33489099108821	21.758887333934915
92-93	21.69676701288322	28.157420556998712	28.40072504396592	21.745087386152143
94-95	21.661084107719674	28.167339240682605	28.419205271377233	21.752371380220488
96-97	21.726665431797905	28.42055767700812	28.564613887808893	21.288163003385083
98-99	22.69126889837855	30.37068922670741	28.161217409325083	18.776824465588955
100	21.744402673528356	31.035684627714165	22.590722001816538	24.62919069694094
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2876.0
1	2583.0
2	3538.5
3	5192.0
4	7583.0
5	12911.0
6	16927.0
7	19541.5
8	21545.0
9	21400.0
10	19643.0
11	18005.5
12	20191.5
13	27211.5
14	44122.0
15	68768.5
16	89981.0
17	101656.5
18	101397.5
19	91713.5
20	77601.5
21	62654.5
22	51880.5
23	49321.5
24	54096.0
25	64781.5
26	80702.0
27	97876.5
28	113540.0
29	131160.5
30	149600.0
31	167923.5
32	188563.0
33	209591.0
34	231800.5
35	252189.5
36	272198.5
37	290507.5
38	302636.0
39	312779.0
40	322451.5
41	329739.0
42	333061.0
43	337323.5
44	341456.0
45	343238.0
46	343153.5
47	340773.0
48	336571.0
49	329437.0
50	319673.0
51	303469.5
52	281731.0
53	259478.0
54	236285.0
55	213310.5
56	191257.5
57	169585.5
58	149463.0
59	129806.5
60	110642.0
61	90895.5
62	71794.5
63	54714.5
64	40156.0
65	30269.5
66	22697.5
67	17576.5
68	13253.5
69	8821.5
70	6039.0
71	4201.0
72	2898.0
73	2031.5
74	1541.5
75	1149.5
76	750.5
77	450.0
78	299.5
79	218.5
80	172.5
81	136.0
82	108.5
83	84.0
84	58.0
85	34.5
86	23.0
87	19.0
88	15.5
89	11.5
90	8.5
91	5.5
92	3.5
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.554107549500446E-4
2	9.804434619311573E-4
3	0.006568971194938755
4	0.026079796087368785
5	0.0022917865922640807
6	0.0
7	0.0
8	0.0
9	0.0
10-11	6.127771637069734E-6
12-13	1.2255543274139468E-5
14-15	1.2255543274139468E-5
16-17	2.4511086548278936E-5
18-19	1.2255543274139468E-5
20-21	1.1643122842422098E-4
22-23	3.431922035066031E-4
24-25	0.007459535391695178
26-27	0.04575987232609332
28-29	0.08796743977503997
30-31	0.07966327024117072
32-33	0.033741831646940466
34-35	0.0019869523462595624
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.428651678613003E-5
56-57	8.312038928603118E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	7.288640893692331E-6
68-69	2.2109815918094628E-5
70-71	8.422957702962533E-4
72-73	0.014176731002596353
74-75	0.021368851284450797
76-77	0.0028188437703591186
78-79	7.011236207145571E-5
80-81	5.124909417226051E-4
82-83	0.009830747562122225
84-85	0.04362955600467912
86-87	0.040450615444144654
88-89	0.009607438093467216
90-91	7.551935288305618E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	141.0
20-21	507.0
22-23	1088.0
24-25	2374.0
26-27	4798.0
28-29	11196.0
30-31	22568.0
32-33	29753.0
34-35	42115.0
36-37	57110.0
38-39	67310.0
40-41	72554.0
42-43	90309.0
44-45	109328.0
46-47	106752.0
48-49	89399.0
50-51	74195.0
52-53	68910.0
54-55	72095.0
56-57	74460.0
58-59	73353.0
60-61	71223.0
62-63	68115.0
64-65	71232.0
66-67	75442.0
68-69	76312.0
70-71	75152.0
72-73	70870.0
74-75	71391.0
76-77	72143.0
78-79	76509.0
80-81	76382.0
82-83	73324.0
84-85	72775.0
86-87	76033.0
88-89	82209.0
90-91	85307.0
92-93	88946.0
94-95	110592.0
96-97	356783.0
98-99	1356215.0
100-101	3982303.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.97027372817716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.00492883199233	77.37748368571485
2	7.130457262015037	12.260147252450029
3	1.693456959947711	4.367608751807745
4	0.5821399604012351	2.001869269752176
5	0.24908052590630153	1.0706760496261534
6	0.11716296711574807	0.6043519412247768
7	0.07031218021438769	0.42313301656190827
8	0.04360787451223326	0.29991847268165545
9	0.02654354591660847	0.20537603173505425
>10	0.07907932157924112	1.0209546908220206
>50	0.0020641998848351956	0.12472536898168608
>100	0.0010354707894734217	0.1688450325090334
>500	1.0908353409562558E-4	0.05356674680432666
>1k	2.1816190601666763E-5	0.021343689328583076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4511086548278936E-5
2	0.0	0.0	0.0	3.67666298224184E-5	2.4511086548278936E-5
3	0.0	0.0	0.0	3.67666298224184E-5	2.4511086548278936E-5
4	0.0	0.0	0.0	7.35332596448368E-5	2.4511086548278936E-5
5	0.0	0.0	0.0	7.35332596448368E-5	2.4511086548278936E-5
6	0.0	0.0	0.0	7.35332596448368E-5	2.4511086548278936E-5
7	0.0	0.0	0.0	1.593220625638131E-4	2.4511086548278936E-5
8	0.0	0.0	0.0	1.593220625638131E-4	2.4511086548278936E-5
9	0.0	0.0	0.0	1.593220625638131E-4	2.4511086548278936E-5
10-11	0.0	0.0	0.0	1.7157760583795255E-4	2.4511086548278936E-5
12-13	0.0	0.0	0.0	2.3898309384571962E-4	2.4511086548278936E-5
14-15	0.0	0.0	0.0	2.6349418039399853E-4	2.4511086548278936E-5
16-17	0.0	0.0	0.0	2.880052669422775E-4	3.67666298224184E-5
18-19	0.0	0.0	0.0	3.3089966840176563E-4	3.67666298224184E-5
20-21	0.0	0.0	0.0	3.492829833129748E-4	4.2894401459488137E-5
22-23	0.0	0.0	0.0	3.7379406986125376E-4	4.902217309655787E-5
24-25	0.0	0.0	0.0	4.1056069968367214E-4	4.902217309655787E-5
26-27	0.0	0.0	0.0	5.086050458767879E-4	4.902217309655787E-5
28-29	0.0	0.0	0.0	9.865712335682271E-4	4.902217309655787E-5
30-31	0.0	0.0	0.0	0.0021079534431519883	4.902217309655787E-5
32-33	0.0	0.0	0.0	0.004111734768473792	4.902217309655787E-5
34-35	0.0	0.0	0.0	0.006930509721525869	4.902217309655787E-5
36-37	0.0	0.0	0.0	0.011121905521281568	4.902217309655787E-5
38-39	0.0	0.0	0.0	0.01655111119172535	4.902217309655787E-5
40-41	0.0	0.0	0.0	0.02210287229491053	4.902217309655787E-5
42-43	0.0	0.0	0.0	0.027961021979949194	4.902217309655787E-5
44-45	0.0	0.0	0.0	0.03455450426143623	4.902217309655787E-5
46-47	0.0	0.0	0.0	0.04147888621132503	4.902217309655787E-5
48-49	0.0	0.0	0.0	0.04815202952409397	6.127771637069734E-5
50-51	0.0	0.0	0.0	0.05475163957721807	6.127771637069734E-5
52-53	0.0	0.0	0.0	0.06144316620489822	6.127771637069734E-5
54-55	0.0	0.0	0.0	0.06843495364279478	6.127771637069734E-5
56-57	1.2255543274139468E-5	0.0	0.0	0.07591083504001986	6.127771637069734E-5
58-59	1.2255543274139468E-5	0.0	0.0	0.08326416100450354	6.127771637069734E-5
60-61	1.2255543274139468E-5	0.0	0.0	0.0907461701733657	6.127771637069734E-5
62-63	1.2255543274139468E-5	0.0	0.0	0.09729063028175616	6.127771637069734E-5
64-65	1.2255543274139468E-5	0.0	0.0	0.10398215690943631	6.127771637069734E-5
66-67	1.2255543274139468E-5	0.0	0.0	0.11048985038800438	6.127771637069734E-5
68-69	1.2255543274139468E-5	0.0	0.0	0.11756742662881992	6.127771637069734E-5
70-71	1.2255543274139468E-5	0.0	0.0	0.12316208213346458	6.127771637069734E-5
72-73	1.2255543274139468E-5	0.0	0.0	0.12750667222414702	6.127771637069734E-5
74-75	1.2255543274139468E-5	0.0	0.0	0.13087694662453536	6.127771637069734E-5
76-77	1.2255543274139468E-5	0.0	0.0	0.13408177119072284	6.127771637069734E-5
78-79	1.2255543274139468E-5	0.0	0.0	0.13625100235024554	6.127771637069734E-5
80-81	1.2255543274139468E-5	0.0	0.0	0.13680862956921888	6.127771637069734E-5
82-83	1.2255543274139468E-5	0.0	0.0	0.1371885514107172	6.127771637069734E-5
84-85	1.2255543274139468E-5	0.0	0.0	0.13743979004783707	6.127771637069734E-5
86-87	1.2255543274139468E-5	0.0	0.0	0.13750719553584484	6.127771637069734E-5
88	1.2255543274139468E-5	0.0	0.0	0.1375317066223931	6.127771637069734E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20340	0.0	58.333397	1
GTATCAA	39055	0.0	38.845158	1
TGGTATC	6660	0.0	35.5187	2
GTGGTAT	7400	0.0	33.254463	1
ATCAACG	47330	0.0	31.668097	3
TCAACGC	49985	0.0	29.983625	4
TATCAAC	50565	0.0	29.740744	2
CAACGCA	50965	0.0	29.415035	5
AACGCAG	51520	0.0	29.097092	6
ACGCAGA	51805	0.0	28.929024	7
CGCAGAG	60165	0.0	24.936832	8
GCAGAGT	66705	0.0	22.467104	9
GAGTACT	36840	0.0	21.453068	12-13
TACATGG	52355	0.0	21.269943	2
GTACATG	53365	0.0	21.069223	1
ACATGGG	51665	0.0	21.056597	3
CAGAGTA	66375	0.0	20.133131	10-11
GTACTTT	40150	0.0	19.587015	14-15
AGAGTAC	64200	0.0	18.58662	10-11
CATGGGG	30640	0.0	18.238052	4
>>END_MODULE