FastQCFastQC Report
Fri 27 May 2016
SRR2139301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12148114
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143100.046.2438131
TATCTCG188050.043.9173694-95
CCGTCTT71650.036.64185792-93
TATGCCG69350.035.72764288-89
TGCCGTC74850.033.80414690-91
GCCGTCT74700.032.8994290-91
CTATCTC234750.032.44320794-95
ATGCCGT75450.032.10665588-89
GTATCAA235900.031.895091
CGTATGC80550.030.96665886-87
ATCTCGT139400.029.13961694-95
CTCGTAT84350.029.1383284-85
TCGTATG82650.028.81280384-85
CGCTATC245300.027.24954692-93
TCTCGTA91300.026.02166482-83
GCTATCT287450.025.94647494-95
ATCAACG284200.025.5918713
TCAACGC287300.025.3011934
CAACGCA292700.024.8201395
AACGCAG301200.024.1751826
Produced by FastQC (version 0.11.5)