##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139301_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12148114
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00367447984107	33.0	31.0	34.0	30.0	34.0
2	32.15666728185132	33.0	31.0	34.0	30.0	34.0
3	32.22331622834623	34.0	31.0	34.0	30.0	34.0
4	35.881748804793894	37.0	35.0	37.0	35.0	37.0
5	35.76066515345509	37.0	35.0	37.0	35.0	37.0
6	35.77567645479784	37.0	35.0	37.0	33.0	37.0
7	35.65605731062451	37.0	35.0	37.0	33.0	37.0
8	35.64665436955893	37.0	35.0	37.0	33.0	37.0
9	37.22686945479767	39.0	37.0	39.0	33.0	39.0
10-11	37.14633246773944	39.0	37.0	39.0	33.0	39.0
12-13	37.10651995033962	39.0	37.0	39.0	33.0	39.0
14-15	38.30130582409747	40.0	38.0	41.0	33.0	41.0
16-17	38.30303975580078	40.0	38.0	41.0	33.0	41.0
18-19	38.34132549299422	40.0	38.0	41.0	33.5	41.0
20-21	38.3124896258298	40.0	38.0	41.0	33.5	41.0
22-23	38.2248797695632	40.0	38.0	41.0	33.0	41.0
24-25	38.15195788791523	40.0	37.5	41.0	33.0	41.0
26-27	37.98169838202233	40.0	37.0	41.0	33.0	41.0
28-29	37.88055148567018	40.0	37.0	41.0	33.0	41.0
30-31	37.761763993281335	40.0	37.0	41.0	32.5	41.0
32-33	37.934022478583024	40.0	37.0	41.0	33.0	41.0
34-35	37.91937495317782	40.0	37.0	41.0	33.0	41.0
36-37	37.847831916754146	40.0	37.0	41.0	33.0	41.0
38-39	37.676177223479186	40.0	37.0	41.0	32.0	41.0
40-41	37.69451042967901	40.0	37.0	41.0	32.5	41.0
42-43	37.66096093465755	40.0	37.0	41.0	32.0	41.0
44-45	37.63446026350434	40.0	37.0	41.0	32.5	41.0
46-47	37.65279095728132	40.0	37.0	41.0	32.0	41.0
48-49	37.56395314521946	40.0	36.5	41.0	32.0	41.0
50-51	37.47934701472698	40.0	36.0	41.0	32.0	41.0
52-53	37.32855269000119	39.0	36.0	41.0	31.0	41.0
54-55	37.232090083858175	39.0	36.0	41.0	31.0	41.0
56-57	37.08952432702449	39.0	35.0	41.0	31.0	41.0
58-59	36.94477123945909	39.0	35.0	40.0	31.0	41.0
60-61	36.742500601827935	38.5	35.0	40.0	31.0	41.0
62-63	36.50060685641054	38.0	35.0	40.0	31.0	41.0
64-65	36.31741185868183	38.0	35.0	40.0	30.5	41.0
66-67	36.03244613875993	37.0	34.0	40.0	30.0	41.0
68-69	35.7558800415625	37.0	34.0	39.5	30.0	41.0
70-71	35.47374350905612	36.0	34.0	39.0	29.5	41.0
72-73	35.1823679538569	36.0	34.0	39.0	29.0	40.0
74-75	34.82812813513679	35.5	34.0	38.5	29.0	40.0
76-77	33.73837712287962	35.0	32.5	37.0	28.0	39.0
78-79	34.031600511432735	35.0	33.0	37.0	29.0	39.0
80-81	33.90659760158891	35.0	33.0	36.5	29.0	39.0
82-83	33.693413622869244	35.0	33.0	36.0	29.0	37.5
84-85	33.510182429703065	35.0	33.0	35.5	29.0	37.0
86-87	33.33162181329696	35.0	33.0	35.0	29.0	37.0
88-89	33.12314192997178	35.0	33.0	35.0	29.0	36.0
90-91	33.046326302171416	35.0	33.0	35.0	29.0	36.0
92-93	33.00684405178511	35.0	33.0	35.0	29.0	36.0
94-95	32.95815667935494	34.0	33.0	35.0	29.0	35.5
96-97	32.86949883525307	34.0	32.0	35.0	29.0	35.0
98-99	32.99678467831299	34.0	32.5	35.0	29.0	35.0
100-101	32.475001060088026	34.0	31.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	3.0
19	23.0
20	85.0
21	570.0
22	2442.0
23	8389.0
24	21829.0
25	45660.0
26	81941.0
27	127461.0
28	179426.0
29	235879.0
30	296640.0
31	364365.0
32	447375.0
33	560743.0
34	735950.0
35	992006.0
36	1489871.0
37	2399317.0
38	2802523.0
39	1300645.0
40	54970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.595858254211315	21.384504623516047	14.009178708727957	25.01045841354469
2	14.59828279417452	22.28487881912506	39.83474803903959	23.282090347660834
3	17.551728035233154	26.554829177856	31.163828624106994	24.729614162803855
4	12.253976213920943	18.29447764484265	38.580243813978036	30.87130232725837
5	12.657223993782079	38.344124857570485	36.08858955390112	12.910061594746312
6	30.272921376931432	38.50197652079985	18.28278858759475	12.942313514673964
7	26.24235333978591	32.731105420973165	23.57630163826253	17.45023960097839
8	24.63585705567136	37.00292078260049	21.148731399787653	17.212490761940497
9	25.724182371024835	15.779288867391267	21.140730157784162	37.355798603799734
10-11	24.297425921422864	26.73362712928114	29.21291733021274	19.75602961908326
12-13	24.72478032392518	24.417070007739472	29.304931613252887	21.55321805508246
14-15	21.9076361115658	25.85095619990709	26.682027625112326	25.559380063414782
16-17	21.62660393210008	28.817181004392943	27.51653466538098	22.039680398125995
18-19	21.53949987627709	28.154201549310454	29.119129109259262	21.18716946515319
20-21	22.376970214605084	27.4553404302392	28.817822872343164	21.34986648281256
22-23	22.09713797610349	27.387948831417425	28.682616883985634	21.832296308493447
24-25	21.859240646016033	27.4578060613672	28.85212022264062	21.830833069976148
26-27	21.863698857347106	27.60864463822913	28.715941358352747	21.811715146071016
28-29	21.78141966191272	27.724429600897132	28.730461671845237	21.763689065344916
30-31	22.064174636356892	27.607142325003714	28.590688175842878	21.737994862796516
32-33	21.88266753962159	27.759556720378566	28.522067611617192	21.83570812838266
34-35	21.981354563621757	27.732192423563788	28.578717266305926	21.707735746508526
36-37	21.95797877556391	27.682707976986375	28.525636075790832	21.833677171658884
38-39	21.864155143493278	27.784124001736068	28.585407121828112	21.766313732942546
40-41	21.984937816811218	27.52533534603925	28.55190875242656	21.937818084722977
42-43	21.997464707349906	27.659655430914476	28.377374137186738	21.96550572454888
44-45	21.940339508285795	27.726547558283933	28.225016746637454	22.108096186792817
46-47	21.97191965794632	27.677619065149628	27.99896025060347	22.35150102630058
48-49	21.91787771707236	27.949273467491803	28.0397274111478	22.093121404288034
50-51	21.897539411885603	28.004266543270752	27.910730856093778	22.187463188749867
52-53	21.95399412294074	27.888687792119327	27.551060426403033	22.606257658536897
54-55	21.917539384423723	27.561405138227276	27.804325615902147	22.716729861446854
56-57	21.952770067152247	27.1636200779062	28.21897692184387	22.664632933097685
58-59	21.744174551723205	26.890801304414385	28.407255268368115	22.957768875494295
60-61	21.82225791034062	26.64841719939424	28.746976225487053	22.782348664778084
62-63	21.54921820599435	27.302426073073278	28.697310752510987	22.45104496842139
64-65	21.01428049107813	27.635010439202652	28.462171950610394	22.888537119108825
66-67	21.1352229969665	28.40163288226349	27.80358115768958	22.65956296308043
68-69	21.304167996991914	28.737272429815246	27.24322314559587	22.71533642759697
70-71	21.239058303405994	28.298182108231718	27.211776657455577	23.250982930906712
72-73	21.14810299507597	27.76169759707432	27.19524339989821	23.8949560079515
74-75	21.73604921919932	27.30428493194099	27.250147369982685	23.709518478877005
76-77	22.213137525331987	27.124641436979886	27.133539126177137	23.528681911510994
78-79	22.513844264533873	26.95092062274171	26.242668690486866	24.292566422237556
80-81	22.354820919409427	27.494578435064586	26.157159261633605	23.99344138389238
82-83	22.250924157955435	27.615489494751973	26.329405267901112	23.804181079391483
84-85	22.337627900147485	27.67124758663461	26.188756401855745	23.802368111362156
86-87	22.690671802287014	28.074346875372473	26.12110117754205	23.11388014479846
88-89	22.984566248498236	27.63479089289122	26.548344606326552	22.83229825228399
90-91	22.551682217184343	27.633232981348343	26.97566994191834	22.83941485954897
92-93	22.301304169799945	26.910397941959218	26.810729757672974	23.97756813056786
94-95	22.079993821825656	26.910559928472406	27.72496222938309	23.284484020318846
96-97	21.524106829321873	27.284142118085448	28.054250009029197	23.137501043563482
98-99	21.718697378888685	27.29364183184808	28.549158739939756	22.43850204932348
100-101	25.89760564641747	34.767139086263846	29.61863602453421	9.716619242784471
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2467.0
1	2301.0
2	3311.0
3	4814.0
4	6489.0
5	10237.0
6	13435.5
7	15828.0
8	17326.5
9	17109.0
10	16035.5
11	14863.5
12	16130.0
13	20766.5
14	31798.0
15	46964.0
16	58523.5
17	63914.0
18	64882.0
19	63099.5
20	59675.5
21	57010.5
22	56866.5
23	61680.0
24	72385.5
25	88235.0
26	109699.5
27	133302.5
28	159517.5
29	187227.5
30	210936.5
31	234691.0
32	261346.5
33	288477.0
34	315042.0
35	339477.0
36	366713.0
37	394948.5
38	416457.0
39	433360.0
40	451439.5
41	468392.5
42	480794.5
43	493804.0
44	506616.0
45	516530.5
46	523589.0
47	527848.0
48	527949.5
49	521988.5
50	511524.0
51	498244.5
52	478161.0
53	453162.0
54	425416.0
55	393984.0
56	361204.0
57	323211.0
58	284838.5
59	248653.5
60	210156.5
61	172553.0
62	137408.5
63	106686.5
64	80023.0
65	59289.5
66	44968.0
67	34294.0
68	26241.0
69	18980.5
70	13349.5
71	9587.0
72	6817.0
73	4693.5
74	3090.5
75	2031.0
76	1396.5
77	915.5
78	623.0
79	430.5
80	304.0
81	224.0
82	168.0
83	123.5
84	90.5
85	63.5
86	43.5
87	32.5
88	21.5
89	16.0
90	14.0
91	13.0
92	11.5
93	8.0
94	4.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00197561530950401
3	9.05490350189338E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.115865228133355E-6
16-17	0.0
18-19	0.0
20-21	0.0020703739449324985
22-23	4.569337661333124E-4
24-25	0.0011488074596227118
26-27	0.011439392332229282
28-29	3.338385698454583E-4
30-31	0.002468121303622051
32-33	0.0025747533266268323
34-35	0.0016336053487148847
36-37	0.06350413322692847
38-39	0.029914589605716087
40-41	0.02636311791316145
42-43	0.0034280383196407483
44-45	0.0015078245696374046
46-47	0.0031021270236332938
48-49	0.0025543779305656107
50-51	5.797632947813214E-4
52-53	9.112972293390557E-4
54-55	0.0012762177592650003
56-57	0.010618597932885639
58-59	0.007783840737514353
60-61	0.003982708635565222
62-63	0.002318255062396762
64-65	0.0010473142775987742
66-67	4.5715846583904794E-4
68-69	0.0018411185263783963
70-71	6.867720492213453E-5
72-73	7.318926400129291E-4
74-75	2.7791279005694733E-4
76-77	0.005818565431153646
78-79	0.0017357153881281135
80-81	5.88332071157863E-4
82-83	3.9883602401230014E-4
84-85	0.002613269481170681
86-87	0.003142702952998481
88-89	0.041583094330304805
90-91	3.2975523830659E-4
92-93	4.548879900354718E-4
94-95	2.537975976244545E-4
96-97	0.020180293865352257
98-99	2.0852904653221409E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	339.0
20-21	1139.0
22-23	2199.0
24-25	5142.0
26-27	5887.0
28-29	11660.0
30-31	32738.0
32-33	45782.0
34-35	65888.0
36-37	100233.0
38-39	69495.0
40-41	110473.0
42-43	122776.0
44-45	142199.0
46-47	143143.0
48-49	132411.0
50-51	123530.0
52-53	19308.0
54-55	18848.0
56-57	18795.0
58-59	19222.0
60-61	138900.0
62-63	136795.0
64-65	143730.0
66-67	152096.0
68-69	154344.0
70-71	152114.0
72-73	146551.0
74-75	150588.0
76-77	149716.0
78-79	158851.0
80-81	158393.0
82-83	150848.0
84-85	151752.0
86-87	158314.0
88-89	167413.0
90-91	178224.0
92-93	183532.0
94-95	219761.0
96-97	272962.0
98-99	1079524.0
100-101	6752499.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.76215669696184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.94832849080795	74.5050382897989
2	7.458566706506713	12.494912664103158
3	1.9569994978877876	4.917674957938575
4	0.7696401934555565	2.578668899380174
5	0.34776338585187366	1.4564705609595323
6	0.18456310864018707	0.9275642415838645
7	0.10259261582768475	0.6015365134036816
8	0.06580061007432139	0.44092808094407954
9	0.042180751819058414	0.31798356691172663
>10	0.12097411964836449	1.5390650371044887
>50	0.002046977367488927	0.11174393304924839
>100	4.7857146070559E-4	0.06286388504104656
>500	4.872928193470808E-5	0.028639445992255902
>1k	1.624137038934242E-5	0.016909923789261917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.23173045626671E-6	0.0	0.0	0.0
3	0.0	8.23173045626671E-6	0.0	0.0	0.0
4	0.0	4.115865228133355E-5	0.0	0.0	0.0
5	0.0	5.7622113193866964E-5	0.0	0.0	0.0
6	0.0	5.7622113193866964E-5	0.0	0.0	0.0
7	0.0	5.7622113193866964E-5	0.0	0.0	8.23173045626671E-6
8	0.0	8.23173045626671E-5	0.0	0.0	1.646346091253342E-5
9	8.23173045626671E-6	1.1524422638773393E-4	0.0	0.0	3.292692182506684E-5
10-11	8.23173045626671E-6	1.2347595684400063E-4	0.0	0.0	3.292692182506684E-5
12-13	8.23173045626671E-6	1.3170768730026735E-4	0.0	4.115865228133355E-6	3.292692182506684E-5
14-15	8.23173045626671E-6	1.7698220480973423E-4	0.0	8.23173045626671E-6	4.527451750946691E-5
16-17	8.23173045626671E-6	1.810980700378676E-4	0.0	2.469519136880013E-5	4.939038273760026E-5
18-19	8.23173045626671E-6	1.9344566572226767E-4	0.0	4.115865228133355E-5	5.762211319386697E-5
20-21	8.23173045626671E-6	2.0579326140666773E-4	0.0	2.881105659693348E-4	6.585384365013367E-5
22-23	8.23173045626671E-6	2.510677789161346E-4	0.0	7.614350672046706E-4	6.585384365013367E-5
24-25	8.23173045626671E-6	2.839947007412015E-4	0.0	0.0017368951262722756	6.585384365013367E-5
26-27	8.23173045626671E-6	2.963422964256015E-4	0.0	0.004634464246878157	6.585384365013367E-5
28-29	8.23173045626671E-6	2.963422964256015E-4	0.0	0.023629182274713587	6.996970887826703E-5
30-31	8.23173045626671E-6	3.375009487069351E-4	0.0	0.038931969192913404	7.408557410640038E-5
32-33	8.23173045626671E-6	3.4573267916320177E-4	0.0	0.054922105604211485	7.408557410640038E-5
34-35	8.23173045626671E-6	3.8689133144453533E-4	0.0	0.07667445333489627	7.408557410640038E-5
36-37	8.23173045626671E-6	4.3216584895400224E-4	0.0	0.10448535468139333	7.408557410640038E-5
38-39	8.23173045626671E-6	4.6097690555093576E-4	0.0	0.1654907090927859	8.23173045626671E-5
40-41	8.23173045626671E-6	5.144831535166694E-4	0.0	0.5655322299412073	8.643316979080044E-5
42-43	8.23173045626671E-6	5.432942101136029E-4	0.0	0.620462567275875	9.466490024706716E-5
44-45	8.23173045626671E-6	6.297273799044032E-4	0.0	0.7106987965374707	1.1524422638773393E-4
46-47	8.23173045626671E-6	6.996970887826703E-4	0.0	0.8143568623080093	1.1524422638773393E-4
48-49	8.23173045626671E-6	7.285081453796038E-4	0.0	0.9008682335381443	1.1524422638773393E-4
50-51	8.23173045626671E-6	8.602158326798711E-4	0.0	0.9825722741818195	1.2347595684400063E-4
52-53	8.23173045626671E-6	8.849110240486712E-4	0.0	1.2609158919647938	1.2347595684400063E-4
54-55	8.23173045626671E-6	8.890268892768047E-4	0.0	2.195921111705076	1.3170768730026735E-4
56-57	8.23173045626671E-6	9.096062154174714E-4	0.0	3.2161988272418256	1.3170768730026735E-4
58-59	8.23173045626671E-6	9.219538111018714E-4	0.0	4.263340795122601	1.3170768730026735E-4
60-61	8.23173045626671E-6	9.301855415581381E-4	0.0	5.046540557653641	1.3170768730026735E-4
62-63	8.23173045626671E-6	9.384172720144049E-4	0.0	5.068412265475941	1.3170768730026735E-4
64-65	8.23173045626671E-6	0.0010536614984021388	0.0	5.082035779381062	1.3170768730026735E-4
66-67	8.23173045626671E-6	0.0010536614984021388	0.0	5.0925353515780305	1.3170768730026735E-4
68-69	8.23173045626671E-6	0.0010824725549990722	0.0	5.101627297866978	1.3170768730026735E-4
70-71	8.23173045626671E-6	0.0010948201506834725	0.0	5.110180065811038	1.3170768730026735E-4
72-73	8.23173045626671E-6	0.0011112836115960058	0.0	5.1173252078470775	1.3170768730026735E-4
74-75	8.23173045626671E-6	0.0011277470725085392	0.0	5.1238323907727565	1.3170768730026735E-4
76-77	8.23173045626671E-6	0.0011647898595617393	0.0	5.130298415046155	1.3170768730026735E-4
78-79	8.23173045626671E-6	0.0011771374552461394	0.0	5.13608532155691	1.3582355252840072E-4
80-81	8.23173045626671E-6	0.0011771374552461394	0.0	5.139485026235349	1.4817114821280076E-4
82-83	8.23173045626671E-6	0.001185369185702406	0.0	5.141835185280613	1.5640287866906748E-4
84-85	8.23173045626671E-6	0.0011936009161586728	0.0	5.143814916455344	1.5640287866906748E-4
86-87	8.23173045626671E-6	0.0012018326466149397	0.0	5.144127722212683	1.5640287866906748E-4
88-89	8.23173045626671E-6	0.0012594547598088065	0.0	5.144156533269279	1.6051874389720085E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14310	0.0	46.243813	1
TATCTCG	18805	0.0	43.91736	94-95
CCGTCTT	7165	0.0	36.641857	92-93
TATGCCG	6935	0.0	35.727642	88-89
TGCCGTC	7485	0.0	33.804146	90-91
GCCGTCT	7470	0.0	32.89942	90-91
CTATCTC	23475	0.0	32.443207	94-95
ATGCCGT	7545	0.0	32.106655	88-89
GTATCAA	23590	0.0	31.89509	1
CGTATGC	8055	0.0	30.966658	86-87
ATCTCGT	13940	0.0	29.139616	94-95
CTCGTAT	8435	0.0	29.13832	84-85
TCGTATG	8265	0.0	28.812803	84-85
CGCTATC	24530	0.0	27.249546	92-93
TCTCGTA	9130	0.0	26.021664	82-83
GCTATCT	28745	0.0	25.946474	94-95
ATCAACG	28420	0.0	25.591871	3
TCAACGC	28730	0.0	25.301193	4
CAACGCA	29270	0.0	24.820139	5
AACGCAG	30120	0.0	24.175182	6
>>END_MODULE