FastQCFastQC Report
Fri 27 May 2016
SRR2139300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12087340
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138200.043.4109041
GTATCAA227450.031.003291
ATCAACG279500.024.5220413
TCAACGC282300.024.3359474
CAACGCA285600.023.9700555
AACGCAG293350.023.5566886
TATCAAC298300.023.2881982
GTACATG341150.021.1903841
TACATGG337150.020.8677772
ACATGGG342600.020.0761853
ACGCAGA348700.019.667177
CGCAGAG353100.019.3764258
CATGGGG247800.017.9946464
AGAGTAC373950.016.25838310-11
GAGTACT238750.016.22816812-13
GCAGAGT421300.016.0196699
GTGGTAT69100.015.9873491
CAGAGTA408300.015.39416910-11
TGGTATC66550.015.3882622
ATGGGGG152050.015.113955
Produced by FastQC (version 0.11.5)