##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139300_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12087340
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.978420562340432	31.0	31.0	34.0	30.0	34.0
2	32.13131069366792	33.0	31.0	34.0	30.0	34.0
3	32.19321703534442	34.0	31.0	34.0	30.0	34.0
4	35.859754503472224	37.0	35.0	37.0	35.0	37.0
5	35.73422705078206	37.0	35.0	37.0	33.0	37.0
6	35.74877830854431	37.0	35.0	37.0	33.0	37.0
7	35.6299479455364	37.0	35.0	37.0	33.0	37.0
8	35.619166748019005	37.0	35.0	37.0	33.0	37.0
9	37.19034800046991	39.0	37.0	39.0	33.0	39.0
10-11	37.11062620063637	39.0	37.0	39.0	33.0	39.0
12-13	37.06991935363777	39.0	37.0	39.0	33.0	39.0
14-15	38.25561935049399	40.0	38.0	41.0	33.0	41.0
16-17	38.256090380513825	40.0	38.0	41.0	33.0	41.0
18-19	38.29280073200555	40.0	38.0	41.0	33.0	41.0
20-21	38.26170045636577	40.0	38.0	41.0	33.0	41.0
22-23	38.17480839649661	40.0	37.5	41.0	33.0	41.0
24-25	38.10094168337676	40.0	37.0	41.0	33.0	41.0
26-27	37.930291180927696	40.0	37.0	41.0	33.0	41.0
28-29	37.827432802777146	40.0	37.0	41.0	32.5	41.0
30-31	37.70658922799139	40.0	37.0	41.0	32.0	41.0
32-33	37.883554004928044	40.0	37.0	41.0	33.0	41.0
34-35	37.86954030656524	40.0	37.0	41.0	33.0	41.0
36-37	37.798614740658934	40.0	37.0	41.0	33.0	41.0
38-39	37.62061491341119	40.0	37.0	41.0	32.0	41.0
40-41	37.6365702172326	40.0	37.0	41.0	32.0	41.0
42-43	37.60783587404859	40.0	37.0	41.0	32.0	41.0
44-45	37.58149247417475	40.0	36.5	41.0	32.0	41.0
46-47	37.59979263862269	40.0	37.0	41.0	32.0	41.0
48-49	37.513042626905374	40.0	36.0	41.0	32.0	41.0
50-51	37.425766878949034	39.5	36.0	41.0	31.5	41.0
52-53	37.28095537332122	39.0	36.0	41.0	31.0	41.0
54-55	37.17289327108506	39.0	35.5	41.0	31.0	41.0
56-57	37.02038935411521	39.0	35.0	40.5	31.0	41.0
58-59	36.86475402822663	39.0	35.0	40.0	31.0	41.0
60-61	36.656821533398684	38.0	35.0	40.0	31.0	41.0
62-63	36.41128224792494	38.0	35.0	40.0	30.0	41.0
64-65	36.204894481219696	38.0	34.5	40.0	30.0	41.0
66-67	35.91316394769218	37.0	34.0	40.0	30.0	41.0
68-69	35.63334193016112	37.0	34.0	39.5	29.0	41.0
70-71	35.35753827435889	36.0	34.0	39.0	29.0	41.0
72-73	35.06716198978641	36.0	34.0	39.0	29.0	40.0
74-75	34.73736449261348	35.0	34.0	38.0	29.0	40.0
76-77	33.68284024212444	35.0	32.0	37.0	27.5	39.0
78-79	34.02285370010081	35.0	33.0	37.0	29.0	39.0
80-81	33.93488389447364	35.0	33.0	36.0	29.0	39.0
82-83	33.73784560713966	35.0	33.0	36.0	29.0	37.5
84-85	33.53830677605367	35.0	33.0	36.0	29.0	37.0
86-87	33.35488476077185	35.0	33.0	35.0	29.0	37.0
88-89	33.15838996047779	35.0	33.0	35.0	29.0	36.0
90-91	33.066775713071664	35.0	33.0	35.0	29.0	36.0
92-93	33.011910459111846	34.5	33.0	35.0	29.0	36.0
94-95	32.97529210977446	34.0	33.0	35.0	29.0	35.5
96-97	32.90902122580901	34.0	32.0	35.0	29.0	35.0
98-99	33.04109311517343	34.0	32.5	35.0	29.0	35.0
100-101	32.552222157253915	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	6.0
19	17.0
20	100.0
21	583.0
22	2604.0
23	8701.0
24	22141.0
25	46200.0
26	81536.0
27	127340.0
28	178521.0
29	233188.0
30	291737.0
31	360972.0
32	443069.0
33	556173.0
34	728823.0
35	983027.0
36	1464376.0
37	2330022.0
38	2693059.0
39	1476499.0
40	58643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.586253054849124	21.473351456978953	13.877197133529792	25.06319835464213
2	14.750619109790733	22.52556090110186	39.368548966159246	23.35527102294816
3	17.539368069087857	26.546879568315063	31.250290593442692	24.66346176915439
4	12.32781571462373	18.310720141900532	38.51408167553821	30.84738246793753
5	12.663811889133589	38.319646837103946	36.14518992598868	12.871351347773786
6	30.287085496064474	38.451131514460585	18.260932512860563	13.000850476614376
7	26.168487028577008	32.79494909549992	23.554380037295218	17.482183838627854
8	24.733977864443293	36.73862901184214	21.245038196989576	17.282354926724985
9	25.752862085454698	15.618035068096042	21.179002162593257	37.450100683856
10-11	24.325711860508598	26.67612146262122	29.226723993864656	19.771442683005525
12-13	24.72282983683755	24.38044681460106	29.280399988748556	21.616323359812828
14-15	21.877398170317043	25.833839372434298	26.693523968052524	25.59523848919613
16-17	21.634702093264522	28.76265166695071	27.5507349011445	22.051911338640263
18-19	21.57304253872233	28.171706926420537	29.079876134865074	21.17537439999206
20-21	22.370273769947296	27.431249730328034	28.819966766898798	21.378509732825872
22-23	22.127904889337223	27.340178258435223	28.65129362911589	21.880623223111662
24-25	21.84927797322239	27.411206417747263	28.84798882370145	21.8915267853289
26-27	21.847386604952156	27.59625822458826	28.681686287423542	21.87466888303604
28-29	21.777345137508842	27.726351347852784	28.697145022974237	21.799158491664137
30-31	22.09513500156884	27.609528128536915	28.55383030191342	21.741506567980835
32-33	21.903990537150715	27.78301873049817	28.47745768334167	21.835533049009452
34-35	21.98705707794672	27.762587038947185	28.534942444030055	21.71541343907604
36-37	21.949859553058797	27.677534968916472	28.52974208436119	21.842863393663546
38-39	21.857754809500584	27.817135397670768	28.54787100047702	21.777238792351625
40-41	22.080753845999183	27.605527450150298	28.4511785524362	21.86254015141432
42-43	21.885475374651246	27.768430145524874	28.264121964497196	22.08197251532668
44-45	22.010004191503782	27.87841736206192	28.085877007292687	22.02570143914161
46-47	22.038607126158183	27.773458665049965	27.873309451133938	22.31462475765791
48-49	22.005466431921995	27.899981785802485	27.924564615499374	22.169987166776146
50-51	22.001949970630264	27.993206293517364	27.752780859390658	22.252062876461707
52-53	22.120685957499138	27.920823107387733	27.57796231823994	22.38052861687319
54-55	22.08018076468928	27.772266729273387	27.69379520709745	22.453757298939877
56-57	22.13308910987611	27.804709096728185	27.690830904368667	22.371370889027038
58-59	22.083851508797782	27.888921026048784	27.687181695953626	22.340045769199808
60-61	22.15721234928553	27.817151227739572	27.701537332464994	22.324099090509904
62-63	22.148322184428782	27.882309497541957	27.605415999623666	22.363952318405595
64-65	22.03680246069499	27.870978990779253	27.6039139935278	22.488304554997953
66-67	22.152045076256886	27.999447654188465	27.350636102369936	22.497871167184712
68-69	22.16342617262976	27.953471343679116	27.391197964371283	22.491904519319842
70-71	22.109430018524325	27.72067576016923	27.505847659491682	22.664046561814764
72-73	22.153354276745777	27.711355261039078	27.48830700204088	22.646983460174262
74-75	22.06790133608211	27.831256072427742	27.41269024747548	22.688152344014668
76-77	22.286846946424607	27.816898874835168	27.286230311539782	22.61002386720044
78-79	22.289266853216972	27.779340755895593	27.26875612547656	22.662636265410875
80-81	22.3323970033118	27.72377006562027	27.232989683084618	22.71084324798331
82-83	22.31528563802963	27.695148385498534	27.451856919987144	22.537709056484694
84-85	22.454225047052578	27.71907912848157	27.29139722758981	22.535298596876043
86-87	22.240850961518735	27.75680045778645	27.38759867330606	22.614749907388756
88-89	22.379312957874482	27.642857839546448	27.407282175354126	22.570547027224936
90-91	22.364037417836638	27.676398381852675	27.486092729105433	22.473471471205254
92-93	22.45335955047366	27.59354891004505	27.449404754122792	22.503686785358497
94-95	22.339328799661043	27.611724720629148	27.576994129303806	22.471952350406003
96-97	22.247592301195603	27.617153969109125	27.686085037971164	22.449168691724104
98-99	22.370272417159153	28.249128340190676	27.775715831000714	21.604883411649457
100-101	26.326564907716243	35.384763840312566	28.56297929260875	9.725691959362438
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2450.0
1	2354.0
2	3480.5
3	5070.5
4	6913.5
5	10804.0
6	14018.0
7	16278.5
8	17551.0
9	17210.0
10	16057.0
11	14856.0
12	16347.5
13	21611.5
14	33156.0
15	48473.0
16	59996.0
17	65216.5
18	66341.0
19	65425.5
20	62989.0
21	60837.0
22	61701.0
23	68134.5
24	81085.0
25	100431.0
26	125564.5
27	152668.5
28	180272.0
29	208945.5
30	233513.5
31	256367.0
32	281482.0
33	307601.5
34	335518.0
35	362471.0
36	389842.0
37	414276.0
38	431780.0
39	446353.5
40	462030.0
41	477484.5
42	489108.0
43	502517.0
44	516467.5
45	527854.5
46	535158.5
47	538318.0
48	537951.0
49	529356.0
50	518130.5
51	506397.0
52	486967.5
53	462882.5
54	435925.5
55	403136.5
56	370776.0
57	336611.0
58	300172.5
59	263999.5
60	224075.0
61	183665.5
62	147176.5
63	115625.0
64	87931.5
65	65882.5
66	49546.0
67	37742.0
68	28830.5
69	20799.0
70	14635.5
71	10427.0
72	7310.5
73	5069.0
74	3533.0
75	2387.5
76	1653.5
77	1129.5
78	765.5
79	531.0
80	385.0
81	280.0
82	197.0
83	136.0
84	99.0
85	70.5
86	47.5
87	37.0
88	32.0
89	24.5
90	17.0
91	13.0
92	10.5
93	5.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018779979714312661
3	4.9638712901266945E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0024241448467086217
22-23	4.7578604819886435E-4
24-25	0.0011132142169291161
26-27	0.011422553842913097
28-29	2.9824474270115375E-4
30-31	0.002489168900108034
32-33	0.002909192127334882
34-35	0.001777084152795619
36-37	0.061807804169972135
38-39	0.028075734570423398
40-41	0.02557745672116075
42-43	0.003334985772464343
44-45	0.0014909743931085491
46-47	0.0033333058160831784
48-49	0.0029103847279666113
50-51	6.971995420866797E-4
52-53	8.87017349362743E-4
54-55	0.001368014651577951
56-57	0.011486560010153529
58-59	0.008822176613598328
60-61	0.0045829745583152144
62-63	0.002550917532217517
64-65	0.0011653939740454149
66-67	4.4080855976413256E-4
68-69	0.0015999181175457237
70-71	6.361189281586896E-5
72-73	7.008283089783815E-4
74-75	2.411971800102794E-4
76-77	0.00561103470770776
78-79	0.0015900856794952888
80-81	5.90315014083643E-4
82-83	3.892837038291833E-4
84-85	0.0029859456883515015
86-87	0.003404866767134732
88-89	0.043328690899193764
90-91	3.832157137858915E-4
92-93	5.033351839072367E-4
94-95	2.680339435505773E-4
96-97	0.0191085508692629
98-99	7.249026383266464E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	391.0
20-21	1197.0
22-23	2270.0
24-25	5008.0
26-27	6025.0
28-29	12441.0
30-31	35046.0
32-33	51006.0
34-35	72853.0
36-37	112265.0
38-39	117967.0
40-41	124078.0
42-43	139205.0
44-45	161167.0
46-47	161432.0
48-49	149100.0
50-51	138226.0
52-53	127498.0
54-55	139063.0
56-57	145302.0
58-59	147763.0
60-61	148224.0
62-63	141633.0
64-65	153410.0
66-67	160284.0
68-69	161957.0
70-71	159607.0
72-73	153216.0
74-75	156176.0
76-77	158171.0
78-79	164449.0
80-81	164648.0
82-83	155446.0
84-85	155788.0
86-87	162727.0
88-89	169868.0
90-91	178995.0
92-93	182190.0
94-95	215305.0
96-97	263095.0
98-99	888459.0
100-101	6144389.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.8176254390033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.5389432587787	77.69837120225044
2	6.519845339017091	11.190352904480005
3	1.6347323179198652	4.2086653725684195
4	0.5931809615902752	2.036215263172082
5	0.2712038614372631	1.1637035699217197
6	0.15246641264267327	0.7850583295318472
7	0.08506003402588888	0.5109755097903825
8	0.0526252556442826	0.36129395820102705
9	0.037451818499163854	0.28926235187737004
>10	0.11202231693121076	1.4859438834324301
>50	0.00167804888812016	0.09703835480596264
>100	7.133501832315969E-4	0.10541317434995878
>500	4.6221708269616605E-5	0.026627417890466885
>1k	3.080273396382501E-5	0.04107870772789854
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.273118816877824E-6	0.0
2	0.0	2.4819356450633472E-5	0.0	8.273118816877824E-6	0.0
3	0.0	2.4819356450633472E-5	0.0	8.273118816877824E-6	0.0
4	0.0	4.9638712901266945E-5	0.0	3.3092475267511294E-5	8.273118816877824E-6
5	0.0	4.9638712901266945E-5	0.0	4.136559408438912E-5	8.273118816877824E-6
6	0.0	5.791183171814477E-5	0.0	4.136559408438912E-5	2.4819356450633472E-5
7	0.0	5.791183171814477E-5	0.0	5.791183171814477E-5	3.3092475267511294E-5
8	8.273118816877824E-6	6.618495053502259E-5	0.0	6.618495053502259E-5	4.9638712901266945E-5
9	1.6546237633755647E-5	1.737354951544343E-4	0.0	9.927742580253389E-5	1.0755054461941171E-4
10-11	1.6546237633755647E-5	1.737354951544343E-4	0.0	1.1996022284472844E-4	1.1996022284472844E-4
12-13	1.6546237633755647E-5	1.737354951544343E-4	0.0	1.40643019886923E-4	1.365064604784841E-4
14-15	3.722903467595021E-5	2.399204456894569E-4	0.0	1.6132581692911757E-4	1.7787205456287322E-4
16-17	4.9638712901266945E-5	2.399204456894569E-4	0.0	2.068279704219456E-4	1.9028173278818995E-4
18-19	5.791183171814477E-5	2.6473980214009035E-4	0.0	2.8955915859072383E-4	2.1923764864726234E-4
20-21	6.204839112658368E-5	2.9783227740760165E-4	0.0	5.708451983645698E-4	2.2751076746414017E-4
22-23	6.618495053502259E-5	3.185150744497962E-4	0.0	0.0010837785650109949	2.5646668332321253E-4
24-25	6.618495053502259E-5	3.3092475267511296E-4	0.0	0.00258534963027432	2.854225991822849E-4
26-27	6.618495053502259E-5	3.433344309004297E-4	0.0	0.006068332652179884	2.8955915859072383E-4
28-29	6.618495053502259E-5	3.474709903088686E-4	0.0	0.02782663514056856	2.8955915859072383E-4
30-31	6.618495053502259E-5	3.805634655763799E-4	0.0	0.0439674899522972	2.9369571799916274E-4
32-33	7.445806935190041E-5	3.9710970321013556E-4	0.0	0.0599346092688714	3.3092475267511296E-4
34-35	7.445806935190041E-5	5.17069926054864E-4	0.0	0.0840135215853943	3.6401722794262426E-4
36-37	7.445806935190041E-5	6.328935894911535E-4	0.0	0.1157450688075292	3.722903467595021E-4
38-39	7.445806935190041E-5	6.783957429839816E-4	0.0	0.16680675814529913	3.847000249848188E-4
40-41	1.3236990107004518E-4	7.445806935190042E-4	0.0	0.2559992521100589	3.8883658439325773E-4
42-43	1.3236990107004518E-4	7.569903717443209E-4	0.0	0.297910872036362	3.8883658439325773E-4
44-45	1.5718925752067865E-4	8.604043569552937E-4	0.0	0.356993350067095	3.9297314380169664E-4
46-47	1.9028173278818995E-4	9.43135545124072E-4	0.0	0.42124652735837664	4.095193814354523E-4
48-49	1.9855485160506778E-4	9.472721045325109E-4	0.0	0.4733919952611575	4.136559408438912E-4
50-51	2.1510108923882343E-4	0.0010093204956590945	0.0	0.5210989349186834	4.2192905966076903E-4
52-53	2.2337420805570125E-4	0.0010175936144759723	0.0	0.5844379325807001	4.508849755198414E-4
54-55	2.2751076746414017E-4	0.0010175936144759723	0.0	0.7041458253015138	4.881140101957916E-4
56-57	2.3164732687257908E-4	0.0010175936144759723	0.0	0.8228940362395696	5.046602478295472E-4
58-59	2.35783886281018E-4	0.001030003292701289	0.0	0.9152592712706021	5.129333666464251E-4
60-61	2.399204456894569E-4	0.0010630957679688003	0.0	0.9705443877643882	5.418892825054974E-4
62-63	2.399204456894569E-4	0.001075505446194117	0.0	1.0054900416468802	5.873914359983255E-4
64-65	2.440570050978958E-4	0.001174782871996651	0.0	1.031066388469258	5.873914359983255E-4
66-67	2.481935645063347E-4	0.001174782871996651	0.0	1.044874223774627	5.915279954067644E-4
68-69	2.481935645063347E-4	0.0012120119066726012	0.0	1.0579622977429277	5.998011142236422E-4
70-71	2.481935645063347E-4	0.00121614846608104	0.0	1.069209602774473	6.246204706742757E-4
72-73	2.523301239147736E-4	0.001220285025489479	0.0	1.0779418796856877	7.03215099434615E-4
74-75	2.6473980214009035E-4	0.0012740602977991849	0.0	1.0854580081308212	7.03215099434615E-4
76-77	2.6473980214009035E-4	0.001319562451292013	0.0	1.09213855157545	7.07351658843054E-4
78-79	2.6473980214009035E-4	0.0013319721295173296	0.0	1.0984385315545024	7.114882182514929E-4
80-81	2.6473980214009035E-4	0.0013319721295173296	0.0	1.1022400296508579	7.197613370683707E-4
82-83	2.6473980214009035E-4	0.0013402452483342075	0.0	1.1047633308900056	7.445806935190042E-4
84-85	2.6473980214009035E-4	0.0013567914859679631	0.0	1.106831610594225	7.445806935190042E-4
86-87	2.6473980214009035E-4	0.0013816108424185966	0.0	1.1071170331934073	7.52853812335882E-4
88-89	2.6887636154852927E-4	0.0014726151494042527	0.0	1.1071666719063087	7.611269311527598E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13820	0.0	43.410904	1
GTATCAA	22745	0.0	31.00329	1
ATCAACG	27950	0.0	24.522041	3
TCAACGC	28230	0.0	24.335947	4
CAACGCA	28560	0.0	23.970055	5
AACGCAG	29335	0.0	23.556688	6
TATCAAC	29830	0.0	23.288198	2
GTACATG	34115	0.0	21.190384	1
TACATGG	33715	0.0	20.867777	2
ACATGGG	34260	0.0	20.076185	3
ACGCAGA	34870	0.0	19.66717	7
CGCAGAG	35310	0.0	19.376425	8
CATGGGG	24780	0.0	17.994646	4
AGAGTAC	37395	0.0	16.258383	10-11
GAGTACT	23875	0.0	16.228168	12-13
GCAGAGT	42130	0.0	16.019669	9
GTGGTAT	6910	0.0	15.987349	1
CAGAGTA	40830	0.0	15.394169	10-11
TGGTATC	6655	0.0	15.388262	2
ATGGGGG	15205	0.0	15.11395	5
>>END_MODULE