FastQCFastQC Report
Fri 27 May 2016
SRR2139299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15709611
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173300.043.7679521
GTATCAA287900.031.6546171
ATCAACG349950.025.3095653
TCAACGC354850.025.0517144
CAACGCA356300.024.8699045
AACGCAG368700.024.1657816
TATCAAC374900.023.9726722
GTACATG452000.022.2396931
TACATGG447050.021.831282
ACATGGG449850.021.2340893
ACGCAGA437850.020.2471457
CGCAGAG442300.020.0434368
CATGGGG324000.018.8809994
GTGGTAT87200.017.2475871
GCAGAGT531450.016.6352889
AGAGTAC471950.016.6095210-11
GAGTACT297450.016.26855312-13
CAGAGTA513150.015.85817410-11
TGGTATC87350.015.7753842
ATGGGGG204000.015.1033225
Produced by FastQC (version 0.11.5)