##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139299_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15709611
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98019034335096	31.0	31.0	34.0	30.0	34.0
2	32.13125684652535	33.0	31.0	34.0	30.0	34.0
3	32.19575557918016	34.0	31.0	34.0	30.0	34.0
4	35.8614378166334	37.0	35.0	37.0	35.0	37.0
5	35.737303106996094	37.0	35.0	37.0	33.0	37.0
6	35.75375106360049	37.0	35.0	37.0	33.0	37.0
7	35.63413766260667	37.0	35.0	37.0	33.0	37.0
8	35.62404957067365	37.0	35.0	37.0	33.0	37.0
9	37.19773023023931	39.0	37.0	39.0	33.0	39.0
10-11	37.11743241127995	39.0	37.0	39.0	33.0	39.0
12-13	37.07813659421612	39.0	37.0	39.0	33.0	39.0
14-15	38.26702020183695	40.0	38.0	41.0	33.0	41.0
16-17	38.26813728869544	40.0	38.0	41.0	33.0	41.0
18-19	38.305961490707816	40.0	38.0	41.0	33.5	41.0
20-21	38.27739662069102	40.0	38.0	41.0	33.0	41.0
22-23	38.19276033675058	40.0	38.0	41.0	33.0	41.0
24-25	38.11819830913781	40.0	37.0	41.0	33.0	41.0
26-27	37.94859469144855	40.0	37.0	41.0	33.0	41.0
28-29	37.84966361485867	40.0	37.0	41.0	33.0	41.0
30-31	37.733495782459364	40.0	37.0	41.0	32.5	41.0
32-33	37.903769257333764	40.0	37.0	41.0	33.0	41.0
34-35	37.88838104636968	40.0	37.0	41.0	33.0	41.0
36-37	37.81711399893072	40.0	37.0	41.0	33.0	41.0
38-39	37.64572277340059	40.0	37.0	41.0	32.0	41.0
40-41	37.66351860923703	40.0	37.0	41.0	32.0	41.0
42-43	37.63535995004311	40.0	37.0	41.0	32.0	41.0
44-45	37.6105992043828	40.0	37.0	41.0	32.0	41.0
46-47	37.629036477110425	40.0	37.0	41.0	32.0	41.0
48-49	37.543098479265645	40.0	36.0	41.0	32.0	41.0
50-51	37.457680629342185	39.5	36.0	41.0	31.5	41.0
52-53	37.311095835166086	39.0	36.0	41.0	31.0	41.0
54-55	37.20692537476685	39.0	36.0	41.0	31.0	41.0
56-57	37.060170435116504	39.0	35.0	41.0	31.0	41.0
58-59	36.908477021841605	39.0	35.0	40.0	31.0	41.0
60-61	36.70308112987273	38.5	35.0	40.0	31.0	41.0
62-63	36.459008831420135	38.0	35.0	40.0	30.5	41.0
64-65	36.25618636095365	38.0	35.0	40.0	30.0	41.0
66-67	35.96483772137787	37.0	34.0	40.0	30.0	41.0
68-69	35.685156127401825	37.0	34.0	39.5	30.0	41.0
70-71	35.40696405848509	36.0	34.0	39.0	29.0	41.0
72-73	35.1160462699161	36.0	34.0	39.0	29.0	40.0
74-75	34.7875134419579	35.0	34.0	38.0	29.0	40.0
76-77	33.72564222807599	35.0	32.5	37.0	28.0	39.0
78-79	34.06394115257325	35.0	33.0	37.0	29.0	39.0
80-81	33.97210895525696	35.0	33.0	36.5	29.0	39.0
82-83	33.77254690420423	35.0	33.0	36.0	29.0	37.5
84-85	33.57060516420016	35.0	33.0	36.0	29.0	37.0
86-87	33.38068380478906	35.0	33.0	35.0	29.0	37.0
88-89	33.17891088079594	35.0	33.0	35.0	29.0	36.0
90-91	33.0856811651757	35.0	33.0	35.0	29.0	36.0
92-93	33.027403989936445	34.5	33.0	35.0	29.0	36.0
94-95	32.99304573875209	34.0	33.0	35.0	29.0	35.5
96-97	32.924647028105085	34.0	32.0	35.0	29.0	35.0
98-99	33.05218423991743	34.0	33.0	35.0	29.0	35.0
100-101	32.55603825327364	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	8.0
19	18.0
20	143.0
21	771.0
22	3403.0
23	11387.0
24	29037.0
25	60083.0
26	105879.0
27	163721.0
28	228892.0
29	298657.0
30	376499.0
31	464444.0
32	574141.0
33	721383.0
34	947090.0
35	1277975.0
36	1905106.0
37	3041373.0
38	3534187.0
39	1889157.0
40	76257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.49871196683355	21.540858013607085	13.959301729368093	25.00112829019127
2	14.676139016233822	22.523718749566342	39.51004315786914	23.290099076330698
3	17.5356395111499	26.644043983039417	31.255987978307026	24.56432852750366
4	12.278668135067125	18.439839153241923	38.65915585051724	30.62233686117371
5	12.659619643032535	38.36211475892051	36.136184403292994	12.842081194753963
6	30.119116253101364	38.540763358176086	18.397145543578386	12.942974845144162
7	26.145504175755846	32.78945608519523	23.61812778177639	17.446911957272526
8	24.724794267662006	36.783737038428264	21.274511507636948	17.216957186272786
9	25.726607743501734	15.730625029480361	21.2201626125561	37.32260461446181
10-11	24.234307902340802	26.762718694944134	29.26711870841359	19.73585469430147
12-13	24.67065225230593	24.440894812735976	29.342292434866785	21.546160500091315
14-15	21.8686955393103	25.888473622930576	26.71439159123673	25.5284392465224
16-17	21.57463988128032	28.86569883875546	27.538552673264793	22.02110860669943
18-19	21.531688467652064	28.250486278749996	29.086076669880622	21.131748583717318
20-21	22.379124282474	27.492658428658643	28.79883426096334	21.32938302790402
22-23	22.087416369209997	27.423558402752622	28.65497824381047	21.83404698422691
24-25	21.824663410175717	27.518520273337376	28.826723935463193	21.830092381023704
26-27	21.8726172188926	27.640251288450006	28.680782150178345	21.806349342479052
28-29	21.750264673566928	27.80178061297436	28.683555206134958	21.764399507323756
30-31	22.06809600361159	27.64710884098614	28.560379911572966	21.724415243829302
32-33	21.91321110485873	27.81496712793114	28.46619453975886	21.805627227451268
34-35	21.989629109846398	27.816423913559323	28.545052995239683	21.648893981354597
36-37	21.939948396416522	27.736772311549178	28.53291607327964	21.790363218754656
38-39	21.874440251922493	27.829382153135246	28.585245045578073	21.710932549364184
40-41	22.040568465540098	27.687559994945605	28.443222134152585	21.828649405361713
42-43	21.919222801588706	27.799450022169754	28.248455438226372	22.032871738015164
44-45	22.038441849124496	27.922111135353518	28.06995610168742	21.969490913834562
46-47	22.056980476499177	27.878089532428184	27.852125739409246	22.212804251663396
48-49	22.061659696085282	27.926953046321785	27.89809516504733	22.113292092545596
50-51	22.036524368231365	28.005077233805704	27.7702902534442	22.188108144518733
52-53	22.141719438263923	27.93787018190625	27.62967917846216	22.290731201367667
54-55	22.111005037159533	27.82227183676232	27.721739883918357	22.344983242159792
56-57	22.13907698928291	27.893273039300198	27.708755769895195	22.2588942015217
58-59	22.08800451583243	27.936402149556887	27.719941674800687	22.255651659809995
60-61	22.182278055816596	27.90017268592227	27.71286009479069	22.204689163470448
62-63	22.17074302482547	27.92518596440978	27.655728857706034	22.24834215305872
64-65	22.05952133028229	27.898475770731775	27.684696982874062	22.357305916111876
66-67	22.184869871576893	27.965253627709874	27.49192389134341	22.35795260936983
68-69	22.11216019880438	27.949926539378783	27.549213147241815	22.388700114575027
70-71	22.111143196251916	27.70883902670061	27.64917677449288	22.530841002554595
72-73	22.17279261584456	27.719826248811092	27.595969638966128	22.51141149637822
74-75	22.028075348505713	27.855422115493823	27.562785571312236	22.553716964688224
76-77	22.176558342064077	27.76914716122205	27.500833422743078	22.553461073970794
78-79	22.172293709905695	27.7554488152976	27.450422346820492	22.621835127976208
80-81	22.19602477844029	27.76876728646605	27.431569211519463	22.603638723574196
82-83	22.255457397371714	27.651356850044913	27.602232466455245	22.49095328612813
84-85	22.323356620681658	27.704167648596822	27.4513261112238	22.52114961949772
86-87	22.13653518341373	27.73398008828628	27.56356838867127	22.565916339628718
88-89	22.26866637615395	27.59422223130532	27.537992292151948	22.599119100388783
90-91	22.24979784391186	27.57160611786695	27.67022868865425	22.508367349566942
92-93	22.23583642576208	27.4997380088763	27.693962727150478	22.57046283821113
94-95	22.062178566837343	27.54369277159506	27.795468193352857	22.59866046821474
96-97	22.04924053342788	27.597366346271972	27.81326603023145	22.540127090068697
98-99	22.183293445158924	28.2599218721608	27.88757309308551	21.669211589594767
100-101	26.176178858573497	35.417261817419885	28.646019071000783	9.760540253005829
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2884.0
1	2728.5
2	4112.0
3	6062.0
4	8273.5
5	12972.0
6	16852.5
7	19639.0
8	21482.5
9	21431.5
10	20167.5
11	18569.5
12	20115.5
13	26426.5
14	40820.5
15	60144.0
16	74598.0
17	81182.5
18	82797.0
19	81528.5
20	78565.5
21	76165.5
22	77228.0
23	85890.0
24	103377.0
25	128954.5
26	162067.5
27	197406.5
28	233378.0
29	272376.0
30	305603.0
31	335569.5
32	369940.5
33	403727.0
34	438137.0
35	473114.0
36	508729.0
37	541351.5
38	565700.5
39	584952.0
40	605274.5
41	624546.5
42	638085.5
43	652348.0
44	669581.5
45	684010.0
46	691090.0
47	695205.5
48	693525.5
49	680614.0
50	665100.5
51	647983.5
52	621278.0
53	589416.0
54	553547.0
55	511194.0
56	469083.0
57	424501.5
58	379074.0
59	332389.0
60	281680.5
61	231593.5
62	184849.0
63	145013.5
64	110186.0
65	82117.5
66	62141.0
67	48198.5
68	37232.0
69	26885.5
70	19093.5
71	13529.5
72	9145.5
73	6128.0
74	4186.0
75	2859.0
76	1951.5
77	1312.5
78	902.0
79	600.0
80	422.5
81	314.0
82	236.0
83	168.0
84	117.5
85	87.5
86	67.0
87	53.0
88	35.5
89	24.0
90	17.5
91	14.0
92	13.0
93	9.5
94	5.5
95	3.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019669487678593695
3	6.365529993072393E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0022089477406888876
22-23	3.819960304882492E-4
24-25	0.0012067771970564953
26-27	0.011311504794233661
28-29	2.58155260118993E-4
30-31	0.00239060454750436
32-33	0.0026379043744938083
34-35	0.001630131203698205
36-37	0.06345795913443344
38-39	0.029140065591115913
40-41	0.026610610497856166
42-43	0.003527979878721444
44-45	0.0017532448769594661
46-47	0.0035102526893166946
48-49	0.0029064896370826787
50-51	7.051663892719845E-4
52-53	0.0010224174250601753
54-55	0.001501633978944936
56-57	0.011561473632634666
58-59	0.008891460237397194
60-61	0.00463978881745093
62-63	0.0026988954201905353
64-65	0.0012989428104303261
66-67	5.578375314963652E-4
68-69	0.0018189955926410492
70-71	4.0274219103028704E-5
72-73	7.07957503725473E-4
74-75	3.0347770904261723E-4
76-77	0.005945271054499248
78-79	0.0015600924399897334
80-81	6.738315378275242E-4
82-83	4.5439959713466303E-4
84-85	0.0029196362858563297
86-87	0.0033933445940050174
88-89	0.043181534909169095
90-91	3.700014545381856E-4
92-93	4.7165549457279285E-4
94-95	3.471711388682543E-4
96-97	0.01996819703142926
98-99	2.1185766250596326E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	484.0
20-21	1538.0
22-23	2874.0
24-25	6402.0
26-27	7717.0
28-29	15477.0
30-31	43106.0
32-33	61438.0
34-35	88547.0
36-37	137174.0
38-39	145575.0
40-41	150575.0
42-43	170664.0
44-45	198556.0
46-47	199098.0
48-49	185307.0
50-51	170858.0
52-53	162594.0
54-55	176557.0
56-57	181793.0
58-59	181977.0
60-61	187634.0
62-63	176632.0
64-65	189900.0
66-67	199873.0
68-69	202005.0
70-71	199253.0
72-73	190981.0
74-75	193793.0
76-77	196148.0
78-79	206210.0
80-81	206204.0
82-83	195518.0
84-85	196298.0
86-87	205385.0
88-89	214204.0
90-91	227567.0
92-93	232539.0
94-95	277875.0
96-97	341131.0
98-99	1176943.0
100-101	8205207.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.55639882651877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.86511583261057	75.98670574933526
2	6.813290913623356	11.522146876268659
3	1.8018263779223689	4.570678494832366
4	0.6756297009052897	2.2851525779515707
5	0.335054555469082	1.416550331044283
6	0.17822353290898316	0.9041964077353905
7	0.09406330200064042	0.5567557855233803
8	0.06381854366914474	0.4317012984812646
9	0.039978178467496614	0.304236972256985
>10	0.12964255884012313	1.7013487205534032
>50	0.0023961412425519326	0.13936396062858006
>100	8.835974621424792E-4	0.10670314642242727
>500	3.840565871813665E-5	0.0240771146676851
>1k	3.8359219550391684E-5	0.05038256429877228
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.3655299930723935E-6
2	0.0	5.7289769937651544E-5	0.0	0.0	6.3655299930723935E-6
3	0.0	6.365529993072393E-5	0.0	0.0	6.3655299930723935E-6
4	0.0	1.2731059986144787E-4	0.0	0.0	6.3655299930723935E-6
5	0.0	1.4004165984759267E-4	0.0	0.0	6.3655299930723935E-6
6	0.0	1.4004165984759267E-4	0.0	6.3655299930723935E-6	1.909658997921718E-5
7	0.0	1.4004165984759267E-4	0.0	6.3655299930723935E-6	1.909658997921718E-5
8	0.0	1.5277271983373745E-4	0.0	6.3655299930723935E-6	2.5462119972289574E-5
9	0.0	3.373730896328369E-4	0.0	1.2731059986144787E-5	5.092423994457915E-5
10-11	3.1827649965361968E-6	3.4055585462937306E-4	0.0	2.8644884968825772E-5	5.092423994457915E-5
12-13	6.3655299930723935E-6	3.4373861962590924E-4	0.0	3.819317995843436E-5	5.092423994457915E-5
14-15	6.3655299930723935E-6	4.073939195566332E-4	0.0	3.819317995843436E-5	9.230018489954971E-5
16-17	6.3655299930723935E-6	4.2330774453931414E-4	0.0	5.7289769937651544E-5	1.2094506986837548E-4
18-19	6.3655299930723935E-6	4.7423198448389337E-4	0.0	1.0503124488569449E-4	1.3685889485105646E-4
20-21	6.3655299930723935E-6	5.315217544215448E-4	0.0	2.959971446778663E-4	1.4004165984759267E-4
22-23	6.3655299930723935E-6	6.397357643037755E-4	0.0	6.747461792656736E-4	1.6550377981988223E-4
24-25	6.3655299930723935E-6	7.097565942275719E-4	0.0	0.0019351211178940076	2.0051419478178038E-4
26-27	6.3655299930723935E-6	7.193048892171805E-4	0.0	0.00497147892458954	2.164280197644614E-4
28-29	6.3655299930723935E-6	7.224876542137167E-4	0.0	0.023787985584111535	2.2597631475407E-4
30-31	6.3655299930723935E-6	8.147878391132664E-4	0.0	0.034450248322507794	2.3552460974367857E-4
32-33	6.3655299930723935E-6	8.275188990994112E-4	0.0	0.03962542420687565	2.4825566972982337E-4
34-35	6.3655299930723935E-6	0.0010121192688985106	0.0	0.04761734711317804	2.5780396471943197E-4
36-37	6.3655299930723935E-6	0.00119671963869761	0.0	0.058639262296182895	2.6098672971596815E-4
38-39	6.3655299930723935E-6	0.001228547288662972	0.0	0.07802548389008487	2.769005546986491E-4
40-41	6.3655299930723935E-6	0.001359040653520956	0.0	0.11945871861499308	2.864488496882577E-4
42-43	6.3655299930723935E-6	0.001406782128468999	0.0	0.13686844314604607	2.9917990967440254E-4
44-45	6.3655299930723935E-6	0.001604113558254243	0.0	0.16202183491367164	3.119109696605473E-4
46-47	6.3655299930723935E-6	0.0017568862780879806	0.0	0.19018612236802043	3.373730896328369E-4
48-49	6.3655299930723935E-6	0.0017664345730775892	0.0	0.21534587966563906	3.6283520960512643E-4
50-51	6.3655299930723935E-6	0.0019796798278455146	0.0	0.23885696469505197	3.6283520960512643E-4
52-53	6.3655299930723935E-6	0.001989228122835123	0.0	0.27036952092575683	3.851145645808798E-4
54-55	6.3655299930723935E-6	0.002021055772800485	0.0	0.3347632223356772	4.073939195566332E-4
56-57	6.3655299930723935E-6	0.0020719800127450643	0.0	0.40061144734901455	4.073939195566332E-4
58-59	6.3655299930723935E-6	0.002087893837727745	0.0	0.46565443281822827	4.073939195566332E-4
60-61	6.3655299930723935E-6	0.002119721487693107	0.0	0.5078006069023606	4.137594495497056E-4
62-63	6.3655299930723935E-6	0.002138818077672324	0.0	0.5297616853784604	4.328560395289228E-4
64-65	6.3655299930723935E-6	0.002317052917478351	0.0	0.5515286151897714	4.36038804525459E-4
66-67	6.3655299930723935E-6	0.0023552460974367857	0.0	0.5636390360015917	4.5195262950813994E-4
68-69	6.3655299930723935E-6	0.00239343927739522	0.0	0.5743840506298978	4.646836894942847E-4
70-71	6.3655299930723935E-6	0.002415718632370973	0.0	0.584689843688682	4.869630444700381E-4
72-73	1.2731059986144787E-5	0.002437997987346727	0.0	0.5929904947996485	5.601666393903707E-4
74-75	1.2731059986144787E-5	0.0025112015822670595	0.0	0.600247198991751	5.601666393903707E-4
76-77	1.2731059986144787E-5	0.0026416949471250433	0.0	0.6072588302791202	5.601666393903707E-4
78-79	1.2731059986144787E-5	0.0026862536570765503	0.0	0.6135829843272376	5.601666393903707E-4
80-81	1.2731059986144787E-5	0.0026862536570765503	0.0	0.6174086678530741	5.824459943661241E-4
82-83	1.2731059986144787E-5	0.002698984717062695	0.0	0.6199803419702754	6.397357643037755E-4
84-85	1.2731059986144787E-5	0.0027085330120523035	0.0	0.6220236770980516	6.46101294296848E-4
86-87	1.2731059986144787E-5	0.0027403606620176654	0.0	0.6223101259477399	6.588323542829927E-4
88-89	1.5913824982680983E-5	0.002813564256937998	0.0	0.6223324053027156	6.715634142691376E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17330	0.0	43.767952	1
GTATCAA	28790	0.0	31.654617	1
ATCAACG	34995	0.0	25.309565	3
TCAACGC	35485	0.0	25.051714	4
CAACGCA	35630	0.0	24.869904	5
AACGCAG	36870	0.0	24.165781	6
TATCAAC	37490	0.0	23.972672	2
GTACATG	45200	0.0	22.239693	1
TACATGG	44705	0.0	21.83128	2
ACATGGG	44985	0.0	21.234089	3
ACGCAGA	43785	0.0	20.247145	7
CGCAGAG	44230	0.0	20.043436	8
CATGGGG	32400	0.0	18.880999	4
GTGGTAT	8720	0.0	17.247587	1
GCAGAGT	53145	0.0	16.635288	9
AGAGTAC	47195	0.0	16.60952	10-11
GAGTACT	29745	0.0	16.268553	12-13
CAGAGTA	51315	0.0	15.858174	10-11
TGGTATC	8735	0.0	15.775384	2
ATGGGGG	20400	0.0	15.103322	5
>>END_MODULE