FastQCFastQC Report
Fri 27 May 2016
SRR2139298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8987937
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105850.046.454131
GTATCAA176850.031.8311481
ATCAACG222250.024.8596973
TCAACGC232950.023.7178274
CAACGCA236300.023.330795
TATCAAC243150.023.0691812
AACGCAG239550.023.047666
ACGCAGA244150.022.5970367
ACATGGG270300.021.150963
GTACATG284550.021.1190621
TACATGG279700.021.0845072
GTGGTAT47100.019.1128231
CGCAGAG296100.018.6729778
CATGGGG194050.017.792674
AGAGTAC308500.016.48292710-11
TGGTATC49000.016.248582
GAGTACT192150.016.08438512-13
GCAGAGT347000.015.7494429
CAGAGTA331150.015.47030110-11
GAGTACA203650.014.9114761
Produced by FastQC (version 0.11.5)