##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8987937
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.121253297614345	33.0	31.0	34.0	30.0	34.0
2	32.25729252441356	33.0	31.0	34.0	30.0	34.0
3	32.974230460226856	34.0	31.0	34.0	30.0	37.0
4	35.8378863803785	37.0	35.0	37.0	35.0	37.0
5	35.74541009800136	37.0	35.0	37.0	33.0	37.0
6	35.74544024952556	37.0	35.0	37.0	33.0	37.0
7	35.705840951043605	37.0	35.0	37.0	33.0	37.0
8	35.94911924727554	37.0	35.0	37.0	33.0	39.0
9	37.19420708000067	39.0	37.0	39.0	33.0	39.0
10-11	37.13522480186499	39.0	37.0	39.0	33.0	39.0
12-13	37.24216458126042	39.0	37.0	39.0	33.0	39.5
14-15	38.27208101258387	40.0	38.0	41.0	33.0	41.0
16-17	38.26893791089101	40.0	38.0	41.0	33.0	41.0
18-19	38.30800772190548	40.0	38.0	41.0	33.5	41.0
20-21	38.2720606206896	40.0	38.0	41.0	33.0	41.0
22-23	38.18994155009376	40.0	38.0	41.0	33.0	41.0
24-25	38.100122890609626	40.0	37.0	41.0	33.0	41.0
26-27	37.933062067071326	40.0	37.0	41.0	33.0	41.0
28-29	37.83441108673024	40.0	37.0	41.0	33.0	41.0
30-31	37.739549426873104	40.0	37.0	41.0	32.5	41.0
32-33	37.89957528021186	40.0	37.0	41.0	33.0	41.0
34-35	37.8801444393515	40.0	37.0	41.0	33.0	41.0
36-37	37.7841829693698	40.0	37.0	41.0	33.0	41.0
38-39	37.64118714550722	40.0	37.0	41.0	32.0	41.0
40-41	37.660976576384044	40.0	37.0	41.0	32.0	41.0
42-43	37.62850934177155	40.0	37.0	41.0	32.0	41.0
44-45	37.61572375973614	40.0	37.0	41.0	32.0	41.0
46-47	37.61749561405348	40.0	37.0	41.0	32.0	41.0
48-49	37.53347290641486	40.0	36.0	41.0	32.0	41.0
50-51	37.44105723545414	39.5	36.0	41.0	31.5	41.0
52-53	37.30658777577445	39.0	36.0	41.0	31.0	41.0
54-55	37.194395725690754	39.0	36.0	41.0	31.0	41.0
56-57	37.047250309304545	39.0	35.0	40.5	31.0	41.0
58-59	36.89261307460434	39.0	35.0	40.0	31.0	41.0
60-61	36.68320724873214	38.0	35.0	40.0	31.0	41.0
62-63	36.445395052308015	38.0	35.0	40.0	30.5	41.0
64-65	36.22202865188744	37.5	35.0	40.0	30.0	41.0
66-67	35.93066771022396	37.0	34.0	40.0	30.0	41.0
68-69	35.645264300636754	36.5	34.0	39.0	30.0	41.0
70-71	35.365354773689	36.0	34.0	39.0	29.0	40.5
72-73	35.06449025004508	36.0	34.0	39.0	29.0	40.0
74-75	34.63064865546633	35.0	33.5	37.5	29.0	40.0
76-77	33.793016670060496	35.0	32.5	37.0	28.5	39.0
78-79	34.06720581961906	35.0	33.0	37.0	29.0	39.0
80-81	33.95911073919685	35.0	33.0	36.0	29.0	39.0
82-83	33.756914925749726	35.0	33.0	36.0	29.0	37.5
84-85	33.557343995112745	35.0	33.0	35.5	29.0	37.0
86-87	33.365782626522865	35.0	33.0	35.0	29.0	37.0
88-89	33.18035015833182	35.0	33.0	35.0	29.0	36.0
90-91	33.094640606418324	35.0	33.0	35.0	29.0	36.0
92-93	33.037706163792755	34.5	33.0	35.0	29.0	36.0
94-95	32.99519901295957	34.0	33.0	35.0	29.0	35.5
96-97	32.945338506852494	34.0	32.5	35.0	29.0	35.0
98-99	33.05866067287704	34.0	33.0	35.0	29.0	35.0
100-101	32.48168545721727	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0
19	14.0
20	104.0
21	446.0
22	1993.0
23	6413.0
24	16230.0
25	33438.0
26	58585.0
27	90717.0
28	128192.0
29	168357.0
30	213805.0
31	264963.0
32	326578.0
33	411949.0
34	540234.0
35	726517.0
36	1082306.0
37	1727695.0
38	1995052.0
39	1127491.0
40	66855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0777744448974	0.010736671395994253	24.520599277605594	31.39088960610101
2	14.881155963119017	25.43766404126469	36.47156210119684	23.209617894419456
3	16.120779258058636	25.079033735672734	33.69007827605321	25.11010873021542
4	13.006466333709282	23.816043659407047	36.921576108065736	26.25591389881794
5	17.148295543237563	37.20414373175958	32.24013474949813	13.407425975504724
6	28.066295969809314	37.4835960688198	20.33289730446486	14.117210656906028
7	26.037754826274373	32.89415580015748	22.752039761738427	18.316049611829722
8	24.556714182576044	31.78020718213757	22.44564019529732	21.217438439989063
9	23.870238520808503	19.906915235387164	23.848431514373097	32.37441472943124
10-11	24.93701974863752	25.919275241875596	28.841931626909023	20.30177338257786
12-13	24.78871402859188	24.132284193803315	28.77047869828193	22.308523079322875
14-15	22.2355697419775	26.719379541712406	26.52935262007288	24.515698096237212
16-17	21.5544882359247	28.694626257609436	27.717266106326644	22.03361940013922
18-19	21.54887184081382	28.016311011362365	29.215521150842978	21.219295996980836
20-21	22.415471633682195	27.45195977694807	28.8932040217711	21.23936456759863
22-23	22.20023029942542	27.175838535564957	28.68930470884149	21.934626456168132
24-25	21.88079837322036	27.40452451565486	28.940642413208593	21.774034697916182
26-27	21.825203612206145	27.665224983781695	28.723829690997064	21.785741713015092
28-29	21.827470378982166	27.59519585453718	28.717093106665537	21.860240659815116
30-31	22.07730556842402	27.51438935054039	28.719100980539753	21.689204100495832
32-33	21.91664157443907	27.831340553730406	28.55351475840486	21.69850311342567
34-35	22.061580707621896	27.62873015314205	28.523620489350463	21.786068649885586
36-37	22.00738151728816	27.64683028479104	28.611747831231348	21.734040366689452
38-39	21.815326464869763	27.921958446707617	28.54272944749195	21.71998564093067
40-41	22.095572859924104	27.570778327149302	28.416883808995326	21.916765003931268
42-43	21.976651390693412	27.58520075894446	28.43245336228103	22.005694488081097
44-45	22.027427358300415	28.025447873212617	27.997748634400775	21.94937613408619
46-47	22.17767610099121	27.730755046696885	27.81379372648809	22.27777512582382
48-49	22.082922449736543	27.870117237561367	27.922721362217157	22.124238950484937
50-51	22.04913944618148	28.095501205659595	27.670403118520266	22.18495622963866
52-53	22.24655651846187	27.841501852809035	27.57152874756149	22.3404128811676
54-55	22.205919133291136	27.736899570073447	27.763300751039928	22.293880545595492
56-57	22.220623932159537	28.056755639777244	27.53183388118286	22.190786546880364
58-59	22.266833065843503	27.90538377387235	27.575354066993192	22.252429093290957
60-61	22.25382220443996	27.89729293510374	27.649781358659446	22.199103501796852
62-63	22.231780506646544	28.05344368018036	27.52312024286452	22.191655570308576
64-65	22.2658634578438	27.768197500932168	27.555765673520334	22.4101733677037
66-67	22.268686731629035	27.895094207717648	27.44653056620673	22.389688494446595
68-69	22.232251351164937	27.983320517809606	27.51591580186718	22.268512329158284
70-71	22.29310684765737	27.51528599335069	27.616271265507912	22.575335893484027
72-73	22.26198182180248	27.734444877023414	27.563542760088268	22.440030541085846
74-75	22.082222967335564	27.88407909523183	27.55376098446464	22.479936952967975
76-77	22.352587246566607	27.684807255375578	27.394824406693758	22.567781091364058
78-79	22.19518465470199	27.80206997248681	27.48874223389634	22.51400313891486
80-81	22.22412260478042	27.84417709191275	27.4206505271739	22.511049776132925
82-83	22.394942316619943	27.548759320008102	27.572676506269765	22.483621857102186
84-85	22.436240426870775	27.63013468396868	27.530043481962487	22.40358140719806
86-87	22.20905725186966	27.75179170513691	27.509414636167346	22.529736406826093
88-89	22.340098541976797	27.562239138357157	27.507608694544665	22.590053625121378
90-91	22.3321158569347	27.560269939378312	27.62267333945281	22.484940864234176
92-93	22.266794445303937	27.622277969306673	27.59277420504708	22.51815338034231
94-95	22.335902492268907	27.487224961620964	27.59289983519925	22.583972710910878
96-97	22.10424572596018	27.661718724459757	27.766622789893887	22.467412759686177
98-99	22.52265782073403	28.794766758450642	27.764145782526857	20.918429638288476
100-101	26.681395296221456	35.72569254578358	28.554667769893715	9.03824438810125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2105.0
1	1974.5
2	2842.0
3	4092.5
4	5580.5
5	8781.5
6	11284.5
7	13032.0
8	14090.0
9	13915.0
10	13025.5
11	12045.0
12	13239.5
13	16882.0
14	25465.0
15	38387.0
16	49003.0
17	53785.5
18	53963.5
19	51959.0
20	48939.0
21	45991.5
22	45420.0
23	49979.0
24	59954.5
25	74497.5
26	94060.0
27	114152.0
28	132985.5
29	154200.0
30	173014.5
31	189869.5
32	208915.5
33	228471.0
34	249066.5
35	269204.0
36	290723.5
37	309434.5
38	322060.5
39	331749.5
40	342615.5
41	354347.5
42	362728.0
43	371987.5
44	382714.5
45	390883.0
46	395106.0
47	397784.0
48	397971.0
49	393305.5
50	386184.5
51	376834.5
52	363077.5
53	347125.5
54	330131.0
55	307243.5
56	281652.5
57	256744.5
58	231645.5
59	204793.5
60	174866.5
61	144900.5
62	116563.5
63	91729.5
64	70026.5
65	52723.0
66	40250.5
67	31274.0
68	24249.0
69	17521.0
70	12151.5
71	8533.0
72	5810.5
73	3873.5
74	2592.5
75	1759.5
76	1255.5
77	871.0
78	607.0
79	425.0
80	280.5
81	188.5
82	145.5
83	105.5
84	75.0
85	50.5
86	35.5
87	28.0
88	21.0
89	13.0
90	7.5
91	5.5
92	6.0
93	4.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.451751608850841E-4
2	0.0011904845349939592
3	3.3378071074596984E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.563011845766165E-6
12-13	0.0
14-15	0.0
16-17	1.6689035537298492E-5
18-19	2.336464975221789E-4
20-21	0.0020473064651434668
22-23	6.009226499598299E-4
24-25	0.0025546488932860264
26-27	0.009917396605989573
28-29	4.0126713473363307E-4
30-31	0.0027082606360794037
32-33	0.002925239453545096
34-35	0.004100518349453496
36-37	0.06284654851386898
38-39	0.026751152281221144
40-41	0.023059740557334854
42-43	0.0029675628374099886
44-45	0.0017950686610790894
46-47	0.0037293597588443908
48-49	0.002376285406576002
50-51	7.513582352495183E-4
52-53	0.0011010714368856415
54-55	0.0022835001106987275
56-57	0.013340561358652999
58-59	0.008029307102336082
60-61	0.0047092021010778865
62-63	0.0027417164959536323
64-65	0.0010718008308242774
66-67	5.095225933834513E-4
68-69	0.001475963229214535
70-71	2.237216060295715E-4
72-73	5.5030002724352E-4
74-75	8.727447194841826E-4
76-77	0.004848113681665117
78-79	0.001297374538669445
80-81	8.181892920263126E-4
82-83	9.377629192671888E-4
84-85	0.0030364374948117323
86-87	0.011762231889323887
88-89	0.032781102195383174
90-91	4.3371649583441336E-4
92-93	5.591837408540173E-4
94-95	0.002020267652702886
96-97	0.01621161130830333
98-99	1.1035332928971982E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	311.0
20-21	1059.0
22-23	1881.0
24-25	4173.0
26-27	6296.0
28-29	13745.0
30-31	28912.0
32-33	41253.0
34-35	60052.0
36-37	88851.0
38-39	94871.0
40-41	95332.0
42-43	108895.0
44-45	124915.0
46-47	124305.0
48-49	113860.0
50-51	106458.0
52-53	105961.0
54-55	113159.0
56-57	115644.0
58-59	113808.0
60-61	111463.0
62-63	107627.0
64-65	112329.0
66-67	117233.0
68-69	118033.0
70-71	115378.0
72-73	111192.0
74-75	112610.0
76-77	114050.0
78-79	118580.0
80-81	119104.0
82-83	112667.0
84-85	114178.0
86-87	118293.0
88-89	125028.0
90-91	130408.0
92-93	134461.0
94-95	160546.0
96-97	239347.0
98-99	950095.0
100-101	4181574.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.98799026499017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.99224327140432	79.15232371882537
2	6.427140714028133	11.181681077272025
3	1.5157010560078379	3.955433661139354
4	0.4938469973617934	1.7183503119560923
5	0.22402857505605467	0.9743897753027854
6	0.12174018206946825	0.6353960263630222
7	0.06488846758252714	0.39511621704653016
8	0.03923033064436808	0.27300540961477093
9	0.027425142013194192	0.21470921878137386
>10	0.09085133762318442	1.2211263000998698
>50	0.002292869893186296	0.13471928566231084
>100	5.334974235948723E-4	0.07845835884693975
>500	3.8780148491240765E-5	0.020915075046504347
>1k	3.8778743820985E-5	0.044375564043137083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.675614214919397E-5	0.0	0.0	0.0
3	0.0	6.675614214919397E-5	0.0	0.0	0.0
4	0.0	6.675614214919397E-5	0.0	1.1126023691532328E-5	0.0
5	0.0	6.675614214919397E-5	0.0	1.1126023691532328E-5	0.0
6	0.0	6.675614214919397E-5	0.0	1.1126023691532328E-5	1.1126023691532328E-5
7	0.0	6.675614214919397E-5	0.0	1.1126023691532328E-5	1.1126023691532328E-5
8	0.0	8.900818953225862E-5	0.0	1.1126023691532328E-5	1.1126023691532328E-5
9	1.1126023691532328E-5	2.336464975221789E-4	0.0	3.3378071074596984E-5	2.2252047383064655E-5
10-11	2.2252047383064655E-5	2.336464975221789E-4	0.0	7.78821658407263E-5	2.2252047383064655E-5
12-13	2.2252047383064655E-5	2.336464975221789E-4	0.0	8.344517768649246E-5	2.2252047383064655E-5
14-15	2.2252047383064655E-5	3.226546870544375E-4	0.0	9.45712013780248E-5	3.3378071074596984E-5
16-17	3.3378071074596984E-5	3.4490673443750216E-4	0.0	1.0013421322379096E-4	3.3378071074596984E-5
18-19	3.3378071074596984E-5	3.4490673443750216E-4	0.0	1.0013421322379096E-4	3.3378071074596984E-5
20-21	3.3378071074596984E-5	3.504697462832683E-4	0.0	2.169574619848804E-4	3.3378071074596984E-5
22-23	3.3378071074596984E-5	3.782848055120992E-4	0.0	2.447725212137112E-4	4.450409476612932E-5
24-25	4.450409476612931E-5	4.0053685289516384E-4	0.0	2.948396278256067E-4	6.11931303034278E-5
26-27	4.450409476612931E-5	4.227889002782285E-4	0.0	4.2835191212399465E-4	8.900818953225863E-5
28-29	4.450409476612931E-5	4.227889002782285E-4	0.0	0.001012468155929442	1.0013421322379096E-4
30-31	4.450409476612931E-5	4.951080542731886E-4	1.1126023691532328E-5	0.002469977259520177	1.0569722506955712E-4
32-33	5.006710661189548E-5	5.006710661189548E-4	1.1126023691532328E-5	0.005262609206094791	1.223862606068556E-4
34-35	6.675614214919397E-5	6.230573267258105E-4	1.1126023691532328E-5	0.009635136516866996	1.3351228429838794E-4
36-37	7.78821658407263E-5	7.45443587332666E-4	1.1126023691532328E-5	0.01574888653536401	1.390752961441541E-4
38-39	7.78821658407263E-5	7.676956347157307E-4	1.1126023691532328E-5	0.024510630192445718	1.4463830798992026E-4
40-41	7.78821658407263E-5	8.344517768649247E-4	1.1126023691532328E-5	0.03346707926412924	1.557643316814526E-4
42-43	7.78821658407263E-5	0.0010069051440836758	1.1126023691532328E-5	0.04211756268429563	1.557643316814526E-4
44-45	1.3351228429838794E-4	0.0011682324876108945	1.1126023691532328E-5	0.05235350448050537	1.557643316814526E-4
46-47	1.4463830798992026E-4	0.0011682324876108945	1.1126023691532328E-5	0.06340720901804274	1.557643316814526E-4
48-49	1.557643316814526E-4	0.001184921523148193	1.1126023691532328E-5	0.07420501501067486	1.557643316814526E-4
50-51	1.557643316814526E-4	0.0012405516416058547	1.1126023691532328E-5	0.08463009920964065	1.6132734352721875E-4
52-53	1.557643316814526E-4	0.0012461146534516207	1.1126023691532328E-5	0.09517200665736754	1.6689035537298491E-4
54-55	1.557643316814526E-4	0.001257240677143153	1.1126023691532328E-5	0.10628134131336256	1.6689035537298491E-4
56-57	1.557643316814526E-4	0.0012794927245262178	1.1126023691532328E-5	0.11769664162087473	1.7801637906451727E-4
58-59	1.557643316814526E-4	0.0012794927245262178	1.1126023691532328E-5	0.1284165654476661	1.9470541460181576E-4
60-61	1.557643316814526E-4	0.0013351228429838793	1.1126023691532328E-5	0.13916986734553213	2.0026842644758192E-4
62-63	1.557643316814526E-4	0.0013740639259042426	1.1126023691532328E-5	0.14960607756818944	2.0026842644758192E-4
64-65	1.6132734352721875E-4	0.0014463830798992026	1.1126023691532328E-5	0.1604316986200504	2.0026842644758192E-4
66-67	1.7245336721875108E-4	0.0014797611509737997	1.1126023691532328E-5	0.17102923618623495	2.0026842644758192E-4
68-69	1.7801637906451724E-4	0.001524265245739929	1.1126023691532328E-5	0.1816545888116483	2.0026842644758192E-4
70-71	1.7801637906451724E-4	0.0015687693405060583	1.1126023691532328E-5	0.19194616072631573	2.0026842644758192E-4
72-73	1.7801637906451724E-4	0.0015687693405060583	1.1126023691532328E-5	0.20115294533105874	2.1139445013911425E-4
74-75	1.7801637906451724E-4	0.001591021387889123	1.1126023691532328E-5	0.20904685914020094	2.169574619848804E-4
76-77	1.7801637906451724E-4	0.0016522145181925509	1.1126023691532328E-5	0.21629546357523421	2.2252047383064657E-4
78-79	1.7801637906451724E-4	0.0016689035537298493	1.1126023691532328E-5	0.22219225613174637	2.2252047383064657E-4
80-81	1.7801637906451724E-4	0.0016744665655756153	1.1126023691532328E-5	0.22580265081964862	2.336464975221789E-4
82-83	1.7801637906451724E-4	0.001691155601112914	1.1126023691532328E-5	0.22836163626870104	2.447725212137112E-4
84-85	1.7801637906451724E-4	0.0017245336721875108	1.1126023691532328E-5	0.23016961511857503	2.447725212137112E-4
86-87	1.7801637906451724E-4	0.0017245336721875108	1.1126023691532328E-5	0.2304199506516345	2.447725212137112E-4
88-89	1.8357939091028343E-4	0.0017746007787994064	1.1126023691532328E-5	0.23045332872270913	2.447725212137112E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10585	0.0	46.45413	1
GTATCAA	17685	0.0	31.831148	1
ATCAACG	22225	0.0	24.859697	3
TCAACGC	23295	0.0	23.717827	4
CAACGCA	23630	0.0	23.33079	5
TATCAAC	24315	0.0	23.069181	2
AACGCAG	23955	0.0	23.04766	6
ACGCAGA	24415	0.0	22.597036	7
ACATGGG	27030	0.0	21.15096	3
GTACATG	28455	0.0	21.119062	1
TACATGG	27970	0.0	21.084507	2
GTGGTAT	4710	0.0	19.112823	1
CGCAGAG	29610	0.0	18.672977	8
CATGGGG	19405	0.0	17.79267	4
AGAGTAC	30850	0.0	16.482927	10-11
TGGTATC	4900	0.0	16.24858	2
GAGTACT	19215	0.0	16.084385	12-13
GCAGAGT	34700	0.0	15.749442	9
CAGAGTA	33115	0.0	15.470301	10-11
GAGTACA	20365	0.0	14.911476	1
>>END_MODULE