FastQCFastQC Report
Fri 27 May 2016
SRR2139297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14538172
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149500.046.232291
GTATCAA263700.034.3304021
TCAACGC330900.026.58924
ATCAACG337350.026.0567783
CAACGCA340250.025.8464785
AACGCAG348050.025.3143866
TATCAAC351650.025.2892152
TACATGG455600.023.0935332
GTACATG465800.022.9312741
ACATGGG461000.022.1908913
ACGCAGA421500.020.8350147
CGCAGAG426250.020.6028398
CATGGGG322350.020.0286584
AGAGTAC453000.017.29910310-11
GCAGAGT517200.016.8291269
GAGTACT278300.016.1979212-13
ATGGGGG193250.015.9827025
GAGTACA323250.015.7858711
CAGAGTA496700.015.66150210-11
GTGGTAT73800.015.2849161
Produced by FastQC (version 0.11.5)