##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139297_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14538172
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.989869290306924	33.0	31.0	34.0	30.0	34.0
2	32.143308525996254	33.0	31.0	34.0	30.0	34.0
3	32.21145684615645	34.0	31.0	34.0	30.0	34.0
4	35.874904148884745	37.0	35.0	37.0	35.0	37.0
5	35.75504355017949	37.0	35.0	37.0	35.0	37.0
6	35.768049105485886	37.0	35.0	37.0	33.0	37.0
7	35.647921279236485	37.0	35.0	37.0	33.0	37.0
8	35.63649026851519	37.0	35.0	37.0	33.0	37.0
9	37.21159606586028	39.0	37.0	39.0	33.0	39.0
10-11	37.128997648397615	39.0	37.0	39.0	33.0	39.0
12-13	37.088071285715976	39.0	37.0	39.0	33.0	39.0
14-15	38.277953170453614	40.0	38.0	41.0	33.0	41.0
16-17	38.28138465413671	40.0	38.0	41.0	33.0	41.0
18-19	38.31958233813715	40.0	38.0	41.0	33.5	41.0
20-21	38.2885028626401	40.0	38.0	41.0	33.0	41.0
22-23	38.2016304550626	40.0	38.0	41.0	33.0	41.0
24-25	38.12776480356257	40.0	37.0	41.0	33.0	41.0
26-27	37.95844985733838	40.0	37.0	41.0	33.0	41.0
28-29	37.85818707014788	40.0	37.0	41.0	33.0	41.0
30-31	37.741425860988855	40.0	37.0	41.0	32.5	41.0
32-33	37.91290980494861	40.0	37.0	41.0	33.0	41.0
34-35	37.89896263362442	40.0	37.0	41.0	33.0	41.0
36-37	37.82732370117325	40.0	37.0	41.0	33.0	41.0
38-39	37.655626687152434	40.0	37.0	41.0	32.0	41.0
40-41	37.6757120480124	40.0	37.0	41.0	32.5	41.0
42-43	37.64658661612502	40.0	37.0	41.0	32.0	41.0
44-45	37.621447846925165	40.0	37.0	41.0	32.0	41.0
46-47	37.63738942351263	40.0	37.0	41.0	32.0	41.0
48-49	37.54849924167078	40.0	36.0	41.0	32.0	41.0
50-51	37.46299045895258	39.5	36.0	41.0	32.0	41.0
52-53	37.31587365335617	39.0	36.0	41.0	31.0	41.0
54-55	37.208668893708904	39.0	36.0	41.0	31.0	41.0
56-57	37.062829090039514	39.0	35.0	40.5	31.0	41.0
58-59	36.91101595401235	39.0	35.0	40.0	31.0	41.0
60-61	36.699632799086345	38.0	35.0	40.0	31.0	41.0
62-63	36.44924935323235	38.0	35.0	40.0	30.5	41.0
64-65	36.238225456755	38.0	35.0	40.0	30.0	41.0
66-67	35.94042282206608	37.0	34.0	40.0	30.0	41.0
68-69	35.654550181243366	37.0	34.0	39.0	30.0	41.0
70-71	35.375137232301384	36.0	34.0	39.0	29.0	41.0
72-73	35.08155181674229	36.0	34.0	39.0	29.0	40.0
74-75	34.7506012671105	35.0	34.0	38.0	29.0	40.0
76-77	33.70555609038517	35.0	32.5	37.0	28.0	39.0
78-79	34.0450040867556	35.0	33.0	37.0	29.0	39.0
80-81	33.95679097710784	35.0	33.0	36.0	29.0	39.0
82-83	33.761628895690436	35.0	33.0	36.0	29.0	37.5
84-85	33.56461869817693	35.0	33.0	36.0	29.0	37.0
86-87	33.37655095088218	35.0	33.0	35.0	29.0	37.0
88-89	33.179362971521314	35.0	33.0	35.0	29.0	36.0
90-91	33.08570863076005	35.0	33.0	35.0	29.0	36.0
92-93	33.0235031304248	34.5	33.0	35.0	29.0	36.0
94-95	32.98590316181153	34.0	33.0	35.0	29.0	35.5
96-97	32.918795457964535	34.0	32.0	35.0	29.0	35.0
98-99	33.04558450320198	34.0	32.5	35.0	29.0	35.0
100-101	32.55004934516328	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	1.0
18	1.0
19	31.0
20	123.0
21	694.0
22	3056.0
23	10161.0
24	25708.0
25	54304.0
26	96983.0
27	150339.0
28	212274.0
29	278869.0
30	351818.0
31	434279.0
32	536094.0
33	674381.0
34	883676.0
35	1195839.0
36	1789294.0
37	2860758.0
38	3241080.0
39	1669749.0
40	68659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.78117744101528	21.438314252988615	13.545458122245355	25.23505018375075
2	14.556559933734611	22.948999670241367	39.31203021948278	23.182410176541243
3	17.304357186756373	27.466121068236603	31.21270119028089	24.016820554726127
4	12.121833473974581	18.591539569073745	38.933725643086355	30.35290131386532
5	12.505884508726407	38.62319829480626	36.12632317185407	12.744594024613273
6	29.60079850479139	38.84991868303663	18.757839706395	12.791443105776985
7	25.811587591617435	32.88292365780237	24.18248318977104	17.12300556080916
8	24.81862919217079	36.63017606340054	21.6267217088916	16.92447303553707
9	26.1240409041797	15.429051190204657	21.133860570641204	37.313047334974435
10-11	24.254589916806594	26.666395885259853	29.496775798222775	19.582238399710775
12-13	24.78995639892003	24.40157194453333	29.49892875115248	21.309542905394157
14-15	22.022624302422617	25.55981591083116	26.792020344786128	25.625539441960104
16-17	21.676576670024264	28.51561049078247	27.60900751483749	22.19880532435577
18-19	21.69402728210947	27.973767953770256	29.03049984551015	21.301704918610127
20-21	22.570124622046063	27.307213825538767	28.63073620663741	21.49192534577777
22-23	22.268181438086682	27.21704763493283	28.57675285171966	21.938018075260832
24-25	21.956984603620963	27.35194129962763	28.72355095635415	21.967523140397258
26-27	21.98073227065148	27.460806917061408	28.583941961631425	21.974518850655684
28-29	21.956376806188324	27.539137431276817	28.61080007220396	21.89368569033089
30-31	22.225324099305087	27.465870700633673	28.409136805437495	21.899668394623745
32-33	22.02498846111255	27.683335162816764	28.40388879701124	21.88778757905945
34-35	22.12984111047372	27.618186525155686	28.423318570698918	21.828653793671677
36-37	22.129432541253447	27.511096655764383	28.436987460103136	21.922483342879033
38-39	22.02430725397044	27.690294464661232	28.450182359709064	21.835215921659266
40-41	22.18407603591783	27.519457198430853	28.372381916135396	21.92408484951592
42-43	22.03700156484995	27.67061574217798	28.166857546387646	22.125525146584426
44-45	22.142349335037167	27.780443244690005	28.02216775009369	22.055039670179134
46-47	22.263168427126065	27.676528712958167	27.81584518549771	22.244457674418058
48-49	22.17200758402214	27.783523794145655	27.806432605058166	22.238036016774043
50-51	22.143017712421663	27.87676415648943	27.669020621147673	22.311197509941234
52-53	22.298575446928794	27.78131770006076	27.587890180188673	22.332216672821772
54-55	22.27379284915345	27.684708925371726	27.6441088130806	22.397389412394226
56-57	22.267247568159164	27.80415677129236	27.671591077856288	22.25700458269219
58-59	22.272779130040988	27.854850236113933	27.592800100376785	22.279570533468295
60-61	22.328044689925715	27.825046283527232	27.59504417136107	22.251864855185985
62-63	22.351018878642034	27.81168000572414	27.572789938971116	22.26451117666271
64-65	22.26779140115446	27.82934560643361	27.571712966556976	22.331150025854956
66-67	22.342204860034194	27.871624696789947	27.39761991125043	22.388550531925418
68-69	22.27767248355614	27.80811659979251	27.486617773523754	22.4275931431276
70-71	22.312505901410063	27.512181733013925	27.619740226846663	22.55557213872935
72-73	22.366954976612924	27.613111569852013	27.552348724380916	22.467584729154147
74-75	22.10746060792495	27.802452334795074	27.55926609763579	22.53082095964419
76-77	22.27563000774554	27.72845744473446	27.503297973582686	22.49261457393731
78-79	22.266290663556553	27.766626064288197	27.429314346993227	22.537768925162023
80-81	22.32232330398671	27.667733405842178	27.43026015893629	22.579683131234823
82-83	22.306079049133775	27.584843878250126	27.62614972447166	22.482927348144443
84-85	22.41133976685165	27.583463584207657	27.491123865450106	22.514072783490587
86-87	22.209834577493247	27.6451420435264	27.613961324734554	22.531062054245805
88-89	22.33920953707744	27.587825985915405	27.53742603239432	22.535538444612836
90-91	22.34003943517002	27.566159317462084	27.661318469708217	22.43248277765968
92-93	22.383738670718962	27.512844285670624	27.556371242178756	22.54704580143166
94-95	22.180554371809805	27.512871262125504	27.730301681550294	22.576272684514397
96-97	22.18855211755347	27.540990360657432	27.732889411332412	22.53756811045669
98-99	22.316531690929935	28.26252719094115	27.77602221180626	21.644918906322655
100-101	26.247101655504494	35.410028062424594	28.603908041537196	9.738962240533715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3379.0
1	3179.5
2	4659.5
3	6808.0
4	8932.5
5	13679.0
6	17815.5
7	21070.0
8	22990.0
9	22421.5
10	20504.5
11	18323.5
12	19589.5
13	25433.0
14	38759.0
15	56106.0
16	68571.5
17	73532.5
18	73495.0
19	71276.5
20	68059.0
21	66228.0
22	68538.0
23	78018.5
24	95259.5
25	119990.0
26	151694.0
27	184005.5
28	215768.0
29	248688.0
30	276738.0
31	303021.5
32	332262.5
33	364244.0
34	395139.5
35	424789.5
36	458015.5
37	486699.5
38	505564.5
39	519743.0
40	536684.0
41	553208.0
42	566980.0
43	583966.0
44	600851.5
45	612748.0
46	617500.0
47	618464.0
48	617893.5
49	612530.0
50	603568.5
51	591573.5
52	571697.5
53	547031.0
54	520026.0
55	487009.5
56	451603.0
57	412186.5
58	370666.0
59	328290.0
60	279700.0
61	229660.5
62	182607.0
63	142000.0
64	107496.0
65	78957.5
66	58525.0
67	44650.5
68	33803.5
69	23728.0
70	16047.5
71	10681.0
72	6947.5
73	4518.0
74	3058.5
75	2119.0
76	1479.0
77	1000.5
78	666.0
79	462.5
80	328.0
81	232.5
82	172.5
83	120.5
84	79.0
85	50.5
86	38.0
87	31.0
88	23.0
89	17.5
90	12.5
91	9.5
92	9.0
93	6.5
94	2.5
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018296660680586251
3	4.814910705417435E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0023800514269956027
22-23	3.921328113930955E-4
24-25	0.0010837750921028199
26-27	0.011392840491215226
28-29	2.8242264486809295E-4
30-31	0.002535013188276775
32-33	0.002922246356019314
34-35	0.0016835131956020525
36-37	0.06259287093309522
38-39	0.02853168524289965
40-41	0.025889285582995636
42-43	0.0033742643565256333
44-45	0.0015240222925272252
46-47	0.003325347233309265
48-49	0.002746302492567214
50-51	7.422855711252571E-4
52-53	8.842845446233803E-4
54-55	0.0014415857875367739
56-57	0.011119719030841186
58-59	0.008273126541008166
60-61	0.004523862110438974
62-63	0.002736919463951989
64-65	0.0012458578823187315
66-67	4.957400102763988E-4
68-69	0.001673410595883765
70-71	8.579961833755775E-5
72-73	6.576210814330443E-4
74-75	3.136679991224365E-4
76-77	0.005194201048744597
78-79	0.0014878166922477106
80-81	5.874810109484257E-4
82-83	4.234066537226548E-4
84-85	0.002552842403572832
86-87	0.0029683474087735997
88-89	0.03980767564543767
90-91	3.5773989407472895E-4
92-93	4.4766854991048946E-4
94-95	1.6835466772786224E-4
96-97	0.018314969801112295
98-99	1.9216675190769303E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	444.0
20-21	1305.0
22-23	2562.0
24-25	5465.0
26-27	8048.0
28-29	15430.0
30-31	35843.0
32-33	49212.0
34-35	70274.0
36-37	117305.0
38-39	136131.0
40-41	133061.0
42-43	143656.0
44-45	167513.0
46-47	173312.0
48-49	168781.0
50-51	155966.0
52-53	144659.0
54-55	153312.0
56-57	164403.0
58-59	169752.0
60-61	169746.0
62-63	153707.0
64-65	154606.0
66-67	168145.0
68-69	175632.0
70-71	177597.0
72-73	164705.0
74-75	160885.0
76-77	163712.0
78-79	178192.0
80-81	183184.0
82-83	171303.0
84-85	166091.0
86-87	172325.0
88-89	188462.0
90-91	205050.0
92-93	208951.0
94-95	243266.0
96-97	305930.0
98-99	1107588.0
100-101	7902661.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.54572792200716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.74406834337991	75.87477584773413
2	6.984908545794023	11.810883549456083
3	1.755504336384615	4.452611759696322
4	0.7003788338420601	2.368561533133739
5	0.30986527658170776	1.3098892683177281
6	0.16670966306364818	0.8456753889209211
7	0.10180104565889242	0.6024790455916782
8	0.06846509485663793	0.4630745025522978
9	0.0410849268689317	0.31261995438806345
>10	0.1229815489541328	1.5932856610621975
>50	0.0031346438297212035	0.17437182407989354
>100	0.001027881572592443	0.13109237805251384
>500	5.588874992112173E-5	0.04015102200589876
>1k	1.3970463203316513E-5	0.020528265008598345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.87844386488205E-6
2	0.0	2.75137754595282E-5	0.0	0.0	6.87844386488205E-6
3	0.0	2.75137754595282E-5	0.0	0.0	6.87844386488205E-6
4	0.0	1.8571798435181535E-4	0.0	1.37568877297641E-5	1.37568877297641E-5
5	0.0	1.8571798435181535E-4	0.0	1.37568877297641E-5	1.37568877297641E-5
6	3.439221932441025E-5	1.8571798435181535E-4	0.0	1.37568877297641E-5	2.063533159464615E-5
7	3.439221932441025E-5	1.9259642821669738E-4	0.0	2.75137754595282E-5	2.063533159464615E-5
8	3.439221932441025E-5	2.201102036762256E-4	0.0	2.75137754595282E-5	2.75137754595282E-5
9	3.439221932441025E-5	4.539772950822153E-4	0.0	4.8149107054174346E-5	6.190599478393845E-5
10-11	4.12706631892923E-5	4.539772950822153E-4	0.0	6.190599478393845E-5	6.190599478393845E-5
12-13	4.8149107054174346E-5	4.539772950822153E-4	0.0	7.222366058126151E-5	6.190599478393845E-5
14-15	4.8149107054174346E-5	6.156207259069435E-4	0.0	7.910210444614358E-5	8.254132637858461E-5
16-17	5.158832898661537E-5	6.259383917042666E-4	0.0	1.100551018381128E-4	1.238119895678769E-4
18-19	5.50275509190564E-5	7.050404961504101E-4	0.0	1.2725121150031793E-4	1.3069043343275896E-4
20-21	6.190599478393845E-5	7.841426005965537E-4	0.0	1.6852187468961022E-4	1.37568877297641E-4
22-23	6.190599478393845E-5	8.529270392453742E-4	0.0	2.0291409401402048E-4	1.6164343082472816E-4
24-25	6.190599478393845E-5	9.251506998266357E-4	0.0	2.7857697652772305E-4	2.0635331594646148E-4
26-27	6.534521671637948E-5	9.629821410834869E-4	0.0	3.920713002982768E-4	2.1323175981134354E-4
28-29	6.87844386488205E-5	9.767390288132512E-4	0.0	8.3917015151561E-4	2.1323175981134354E-4
30-31	7.222366058126151E-5	0.001090233352583805	0.0	0.002042897827869969	2.1323175981134354E-4
32-33	7.566288251370255E-5	0.00110742946224601	0.0	0.004553529838551917	2.441847572033128E-4
34-35	7.910210444614358E-5	0.0013447357755844405	0.0	0.007906771222681916	2.5450242300063586E-4
36-37	8.25413263785846E-5	0.0015751636450579893	0.0	0.013203172998641094	2.5450242300063586E-4
38-39	8.25413263785846E-5	0.0016129950863148406	0.0	0.02107211278006616	2.613808668655179E-4
40-41	8.25413263785846E-5	0.0017780777390720098	0.0	0.029725195162087777	2.613808668655179E-4
42-43	8.25413263785846E-5	0.0018606190654505946	0.0	0.03722269897480922	2.71698532662841E-4
44-45	8.941977024346664E-5	0.0021219999323161126	0.0	0.04588265980069571	2.8201619846016405E-4
46-47	9.629821410834869E-5	0.002311157138600369	0.0	0.055223586569205535	2.923338642574871E-4
48-49	9.629821410834869E-5	0.0023283532482625737	0.0	0.06520764783908184	3.0952997391969225E-4
50-51	9.973743604078972E-5	0.0025690987835334453	0.0	0.0752054659966879	3.0952997391969225E-4
52-53	1.0317665797323075E-4	0.0025794164493307685	0.0	0.0843262825615215	3.9894974416315894E-4
54-55	1.0317665797323075E-4	0.002593173337060533	0.0	0.09343334223862532	4.986871802039486E-4
56-57	1.0317665797323075E-4	0.002648200887979589	0.0	0.10234780548751246	5.158832898661538E-4
58-59	1.0317665797323075E-4	0.0026688362195742355	0.0	0.11086675821416889	5.227617337310358E-4
60-61	1.100551018381128E-4	0.0027135461046959687	0.0	0.11959894270063665	5.227617337310358E-4
62-63	1.37568877297641E-4	0.00278576976527723	0.0	0.12815916609048236	5.365186214607999E-4
64-65	1.37568877297641E-4	0.0031090566269266866	0.0	0.1365646244933682	5.365186214607999E-4
66-67	1.478865430949641E-4	0.0031331311804537734	0.0	0.14486346701634842	5.468362872581229E-4
68-69	1.513257650274051E-4	0.0032019156191025943	0.0	0.15363348294407303	5.57153953055446E-4
70-71	1.5476498695984612E-4	0.003250064726156769	0.0	0.16228312610416218	5.640323969203281E-4
72-73	1.7196109662205125E-4	0.0032672608358189736	0.0	0.1700454500056816	5.984246162447384E-4
74-75	1.7196109662205125E-4	0.003349802162197558	0.0	0.17643896357808947	5.984246162447384E-4
76-77	1.8915720628425638E-4	0.003470174929832994	0.0	0.18216182887367133	5.984246162447384E-4
78-79	2.063533159464615E-4	0.0035148848149547276	0.0	0.18772992918229336	5.984246162447384E-4
80-81	2.063533159464615E-4	0.0035148848149547276	0.0	0.190955919354923	6.224991697718255E-4
82-83	2.063533159464615E-4	0.0035148848149547276	0.0	0.19313294683815818	6.740874987584409E-4
84-85	2.063533159464615E-4	0.0035458378123466965	0.0	0.19506922878612248	6.740874987584409E-4
86-87	2.0979253787890254E-4	0.003618061472927958	0.0	0.19533060965298799	6.775267206908819E-4
88-89	2.1323175981134354E-4	0.00375906957215804	0.0	0.19536156265038	6.844051645557639E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14950	0.0	46.23229	1
GTATCAA	26370	0.0	34.330402	1
TCAACGC	33090	0.0	26.5892	4
ATCAACG	33735	0.0	26.056778	3
CAACGCA	34025	0.0	25.846478	5
AACGCAG	34805	0.0	25.314386	6
TATCAAC	35165	0.0	25.289215	2
TACATGG	45560	0.0	23.093533	2
GTACATG	46580	0.0	22.931274	1
ACATGGG	46100	0.0	22.190891	3
ACGCAGA	42150	0.0	20.835014	7
CGCAGAG	42625	0.0	20.602839	8
CATGGGG	32235	0.0	20.028658	4
AGAGTAC	45300	0.0	17.299103	10-11
GCAGAGT	51720	0.0	16.829126	9
GAGTACT	27830	0.0	16.19792	12-13
ATGGGGG	19325	0.0	15.982702	5
GAGTACA	32325	0.0	15.785871	1
CAGAGTA	49670	0.0	15.661502	10-11
GTGGTAT	7380	0.0	15.284916	1
>>END_MODULE