FastQCFastQC Report
Fri 27 May 2016
SRR2139296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23509273
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA341100.045.711041
GTATCAA634050.033.850311
ATCAACG859500.029.55583
TCAACGC866200.029.3919724
CAACGCA872700.029.11375
TATCAAC891350.028.7330112
AACGCAG904800.028.1806496
ACGCAGA1015650.025.0157097
CGCAGAG1038500.024.4694468
TGTATCA161400.023.3488661
GCAGAGT1193550.021.3485689
TTATCAA219600.021.1118851
TACATGG824200.020.232392
GAGTACT676800.020.09031712-13
ACATGGG807400.020.059323
AGAGTAC1066050.019.81024610-11
CAGAGTA1156750.019.0069910-11
CATGGGG553600.018.6434214
AGTACTT728800.018.42587912-13
GTACATG793150.018.0691551
Produced by FastQC (version 0.11.5)