##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23509273
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.332072114692785	18.0	18.0	28.0	18.0	33.0
2	30.184031764827438	32.0	27.0	33.0	27.0	33.0
3	31.913698522280974	33.0	32.0	33.0	30.0	33.0
4	32.250034401319006	33.0	33.0	33.0	31.0	34.0
5	32.758321833261284	33.0	33.0	34.0	32.0	34.0
6	36.59669522745344	38.0	37.0	38.0	34.0	38.0
7	36.91522051745284	38.0	38.0	38.0	35.0	38.0
8	36.94891245679949	38.0	38.0	38.0	35.0	38.0
9	37.108766145171735	38.0	38.0	38.0	36.0	38.0
10-11	37.15031881249582	38.0	38.0	38.0	36.0	38.0
12-13	37.169790554561175	38.0	38.0	38.0	36.0	38.0
14-15	37.16113218388335	38.0	38.0	38.0	36.0	38.0
16-17	37.115710319923544	38.0	38.0	38.0	36.0	38.0
18-19	37.140082660148614	38.0	38.0	38.0	36.0	38.0
20-21	37.156612332847956	38.0	38.0	38.0	36.0	38.0
22-23	37.159199253667786	38.0	38.0	38.0	36.0	38.0
24-25	37.16387863751124	38.0	38.0	38.0	36.0	38.0
26-27	37.13189832893015	38.0	38.0	38.0	36.0	38.0
28-29	37.105092397832166	38.0	38.0	38.0	36.0	38.0
30-31	37.10336238364112	38.0	38.0	38.0	36.0	38.0
32-33	37.092708095180704	38.0	38.0	38.0	36.0	38.0
34-35	37.067168667501846	38.0	38.0	38.0	36.0	38.0
36-37	37.0459245466798	38.0	38.0	38.0	36.0	38.0
38-39	37.026124123777116	38.0	38.0	38.0	36.0	38.0
40-41	36.99850056283613	38.0	38.0	38.0	36.0	38.0
42-43	36.96390955679159	38.0	38.0	38.0	36.0	38.0
44-45	36.903681911989494	38.0	38.0	38.0	35.5	38.0
46-47	36.867828375704356	38.0	38.0	38.0	35.0	38.0
48-49	36.825627330767276	38.0	38.0	38.0	35.0	38.0
50-51	36.77440450194848	38.0	38.0	38.0	35.0	38.0
52-53	36.741456796072924	38.0	38.0	38.0	35.0	38.0
54-55	36.70529261202896	38.0	38.0	38.0	35.0	38.0
56-57	36.70112582687484	38.0	38.0	38.0	35.0	38.0
58-59	36.680356385197214	38.0	38.0	38.0	34.0	38.0
60-61	36.68712518914165	38.0	38.0	38.0	34.5	38.0
62-63	36.671804828868495	38.0	38.0	38.0	34.0	38.0
64-65	36.65895185905339	38.0	38.0	38.0	34.0	38.0
66-67	36.647514667602174	38.0	38.0	38.0	34.0	38.0
68-69	36.65292113180797	38.0	38.0	38.0	34.0	38.0
70-71	36.626132264243935	38.0	38.0	38.0	34.0	38.0
72-73	36.60222901617397	38.0	38.0	38.0	34.0	38.0
74-75	36.61238969148951	38.0	38.0	38.0	34.0	38.0
76-77	36.58176454246468	38.0	38.0	38.0	34.0	38.0
78-79	36.54914391854894	38.0	38.0	38.0	34.0	38.0
80-81	36.53687102000019	38.0	38.0	38.0	34.0	38.0
82-83	36.47018151648855	38.0	38.0	38.0	34.0	38.0
84-85	36.46315747728983	38.0	38.0	38.0	34.0	38.0
86-87	36.44423435993272	38.0	38.0	38.0	34.0	38.0
88-89	36.41318813678696	38.0	38.0	38.0	34.0	38.0
90-91	36.386688228966854	38.0	37.0	38.0	34.0	38.0
92-93	36.384255628866214	38.0	37.5	38.0	34.0	38.0
94-95	36.32226618081238	38.0	37.0	38.0	33.5	38.0
96-97	36.281564699125596	38.0	37.0	38.0	33.0	38.0
98-99	36.31941255992099	38.0	37.0	38.0	33.5	38.0
100	34.52359686936258	38.0	34.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	10.0
18	51.0
19	157.0
20	360.0
21	962.0
22	3016.0
23	8330.0
24	19448.0
25	37698.0
26	64445.0
27	102466.0
28	154081.0
29	218322.0
30	293390.0
31	378979.0
32	489765.0
33	653095.0
34	953898.0
35	1622469.0
36	4385206.0
37	1.4123125E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.17599319213316	17.507419306415812	23.67380309888783	22.642784402563194
2	14.168338709861978	21.86779401278584	41.360238138595975	22.60362913875621
3	17.5836913374565	27.213023558831445	31.278934061465876	23.92435104224618
4	11.900491350795917	18.895986277414874	39.63652555312961	29.566996818659597
5	12.62252808980878	37.78312225557475	36.51989704448034	13.074452610136131
6	28.571972430096586	39.35514088300274	19.05939698056838	13.013489706332301
7	25.14805115411268	33.38610683537513	24.168114428719253	17.297727581792937
8	24.303444006966952	36.5761714537068	21.477227305157413	17.643157234168832
9	25.8242566667204	16.134122905459474	21.14159804090922	36.900022386910905
10-11	23.887025345275458	26.72638792360785	29.441316624295442	19.94527010682125
12-13	25.132587043418997	24.541001331687287	29.3819613222408	20.944450302652914
14-15	22.28179705939865	25.447518092116244	27.14772166710557	25.122963181379536
16-17	21.448311055811892	28.619141051277936	27.67337382147036	22.259174071439812
18-19	21.542599381954517	27.669953894363303	29.67127694676054	21.11616977692164
20-21	22.613913856539185	26.797030097917627	29.372774071295183	21.216281974248005
22-23	22.33824106088683	26.66062059420477	29.325511231517314	21.675627113391087
24-25	21.920010953999583	26.893639063841512	29.42009030922497	21.766259672933927
26-27	21.88334460007945	26.958829671268482	29.340813431408286	21.817012297243785
28-29	21.85049852938228	26.986915463565893	29.420236597272115	21.742349409779713
30-31	22.148152713822725	26.856262687249956	29.231562382733582	21.76402221619373
32-33	21.93750886071562	27.127075941756285	29.138290590582127	21.797124606945964
34-35	22.064193184367507	27.039376608697125	29.22936383119617	21.667066375739203
36-37	22.058213086279142	26.943088382644092	29.195967237694692	21.802731293382074
38-39	21.914379525207067	27.064946571294023	29.257417431160725	21.76325647233818
40-41	22.135817557006714	26.911504398686603	29.121967122757386	21.830710921549297
42-43	22.001607387124494	27.067875360374373	28.903573913224385	22.026943339276748
44-45	22.12254845659851	27.21990745366699	28.692141247901944	21.96540284183256
46-47	22.264974390074972	27.195509191658264	28.351841847294047	22.187674570972714
48-49	22.213260289700816	27.32624460720207	28.281985032354612	22.178510070742504
50-51	22.180463350087457	27.48709073448544	28.066285656019414	22.266160259407688
52-53	22.32814371778222	27.372915285553333	27.976796367537098	22.32214462912735
54-55	22.328739770700377	27.25662705875534	28.052429113491684	22.362204057052598
56-57	22.32906896771579	27.341901589414658	28.058821316354827	22.270208126514724
58-59	22.293848764351928	27.39920798653322	28.027166434484883	22.27977681462997
60-61	22.29155231186512	27.385585157579733	28.06023681857078	22.26262571198437
62-63	22.272853656891336	27.385843389323416	28.061031322852276	22.280271630932972
64-65	22.24608899301182	27.385274095968377	28.001337739005393	22.36729917201441
66-67	22.363099276490715	27.436382670393865	27.80956845264418	22.390949600471245
68-69	22.25500915182714	27.382700163170554	27.949211709870802	22.41307897513151
70-71	22.344138237577653	27.117034184539335	27.984877847148105	22.553949730734907
72-73	22.401838833225547	27.20062108716985	27.90907790739749	22.48846217220711
74-75	22.212442141282096	27.436532118026747	27.882310075769812	22.468715664921348
76-77	22.442445344361925	27.341153080890145	27.727098114397	22.48930346035093
78-79	22.419903762529366	27.37823757175093	27.64405186657692	22.55780679914279
80-81	22.487423815818104	27.38693035184263	27.584673553863652	22.54097227847561
82-83	22.498187724714562	27.305608548182015	27.740844774170814	22.455358952932613
84-85	22.580898498603414	27.396322882690487	27.61703351346478	22.405745105241326
86-87	22.373765240514672	27.421154342838598	27.743494580862677	22.461585835784057
88-89	22.501076942992704	27.37755213025749	27.6395525350188	22.481818391731
90-91	22.608331262535405	27.351023420823477	27.701301934682256	22.33934338195886
92-93	22.617032908717754	27.379576302487248	27.57025721044909	22.433133578345917
94-95	22.41368844124314	27.418319618448738	27.674227439601058	22.493764500707066
96-97	22.571347624966062	27.515794042745835	27.71837856149542	22.19447977079268
98-99	23.52070084090449	29.519197173258473	27.874487924934215	19.085614060902824
100	22.467824446877458	30.244602769466006	21.965970945066047	25.32160183859049
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4404.0
1	6322.5
2	11504.0
3	15227.0
4	18583.0
5	24161.5
6	28167.0
7	35599.0
8	40719.5
9	38907.0
10	37153.0
11	36783.5
12	40486.0
13	52147.0
14	80456.5
15	115348.5
16	135340.5
17	135912.0
18	123481.5
19	106457.0
20	90458.0
21	79420.5
22	77786.0
23	88174.5
24	110351.0
25	145756.5
26	191963.0
27	240719.5
28	287790.5
29	339822.0
30	388289.5
31	431272.0
32	484808.5
33	538909.0
34	588947.5
35	636688.0
36	687036.5
37	738975.0
38	772627.5
39	790612.0
40	817748.0
41	843316.5
42	861672.5
43	886601.5
44	912483.5
45	941125.5
46	964881.5
47	976015.5
48	978482.0
49	965438.0
50	942997.0
51	920242.5
52	887369.5
53	844196.5
54	793868.5
55	734948.5
56	672574.0
57	606293.5
58	537817.5
59	467547.0
60	388153.5
61	310148.0
62	241387.5
63	183019.0
64	135050.5
65	97631.5
66	70867.5
67	52437.5
68	39057.5
69	27843.0
70	19289.0
71	13275.0
72	8904.5
73	5749.5
74	3535.0
75	2007.5
76	1182.0
77	660.5
78	378.5
79	230.0
80	139.0
81	87.5
82	68.5
83	45.5
84	26.5
85	20.0
86	17.0
87	15.5
88	11.0
89	5.0
90	2.5
91	1.5
92	0.5
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002594720815058807
3	0.0
4	0.0
5	0.0020417475266036513
6	5.955096952593983E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.381452877288617E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.0670738734388922E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	6.64373132502489E-6
48-49	3.7956276022236754E-4
50-51	0.0016716568887112989
52-53	0.004277762816553316
54-55	0.006066856485081149
56-57	0.00667338452513883
58-59	0.008360292120886315
60-61	0.00801126814514446
62-63	0.005845471687627739
64-65	0.003386237772790949
66-67	0.0011269339934789038
68-69	6.040298860564735E-4
70-71	2.3694343739453903E-4
72-73	5.219274654475482E-4
74-75	4.917084192675747E-6
76-77	2.6269025000602824E-4
78-79	2.0243432018481606E-4
80-81	1.033996467212363E-4
82-83	2.0361924555149854E-5
84-85	1.7971264512855117E-4
86-87	1.4498234736362028E-4
88-89	2.8732402415684568E-5
90-91	7.91303678085003E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	363.0
20-21	2257.0
22-23	4179.0
24-25	2970.0
26-27	6461.0
28-29	15828.0
30-31	37802.0
32-33	47618.0
34-35	63130.0
36-37	100403.0
38-39	132027.0
40-41	133237.0
42-43	150147.0
44-45	186276.0
46-47	188354.0
48-49	174640.0
50-51	151625.0
52-53	131291.0
54-55	136206.0
56-57	144285.0
58-59	157491.0
60-61	162430.0
62-63	153421.0
64-65	155167.0
66-67	167995.0
68-69	177472.0
70-71	183028.0
72-73	166287.0
74-75	160114.0
76-77	166066.0
78-79	178698.0
80-81	183917.0
82-83	171816.0
84-85	162755.0
86-87	166988.0
88-89	181867.0
90-91	192942.0
92-93	192902.0
94-95	230356.0
96-97	734781.0
98-99	1712774.0
100-101	1.5940907E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.63002958580019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.48284659922855	68.00148786761561
2	8.617448255936864	13.551804226368358
3	2.4091265267740885	5.682890702285479
4	1.0192603437507803	3.2057788393902693
5	0.524706874868067	2.0628858547374436
6	0.2938145710545155	1.386158904885345
7	0.18016954960767936	0.9916715911278488
8	0.12106552488113612	0.761550864257933
9	0.0819111682797935	0.5796609826722859
>10	0.26143951796011977	3.108798508116953
>50	0.005880336831725045	0.3083642179124834
>100	0.002267939022827117	0.2925063179207715
>500	2.5123592533686294E-5	0.015526885240887888
>1k	3.766821131211055E-5	0.05091423746839178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.507281360848547E-6
2	0.0	5.104368816509128E-5	0.0	0.0	8.507281360848547E-6
3	0.0	5.955096952593983E-5	0.0	0.0	8.507281360848547E-6
4	0.0	1.8290654925824375E-4	0.0	4.253640680424273E-6	1.276092204127282E-5
5	1.276092204127282E-5	1.8290654925824375E-4	0.0	4.253640680424273E-6	1.276092204127282E-5
6	1.276092204127282E-5	1.8290654925824375E-4	0.0	4.253640680424273E-6	2.552184408254564E-5
7	1.276092204127282E-5	1.8716018993866803E-4	0.0	4.253640680424273E-6	2.552184408254564E-5
8	1.276092204127282E-5	2.0417475266036513E-4	0.0	4.253640680424273E-6	2.552184408254564E-5
9	1.276092204127282E-5	3.5305217647521467E-4	0.0	4.253640680424273E-6	2.9775484762969914E-5
10-11	1.276092204127282E-5	3.5305217647521467E-4	0.0	1.276092204127282E-5	2.9775484762969914E-5
12-13	1.276092204127282E-5	3.57305817155639E-4	0.0	1.914138306190923E-5	2.9775484762969914E-5
14-15	1.4887742381484957E-5	5.444660070943071E-4	0.0	2.552184408254564E-5	3.190230510318205E-5
16-17	2.552184408254564E-5	5.636073901562162E-4	0.0	2.552184408254564E-5	3.828276612381846E-5
18-19	2.552184408254564E-5	6.018901562800347E-4	0.0	2.7648664422757776E-5	4.679004748466701E-5
20-21	2.9775484762969914E-5	6.486802037647017E-4	0.0	2.9775484762969914E-5	4.891686782487914E-5
22-23	2.9775484762969914E-5	7.401334783938235E-4	0.0	3.4029125443394186E-5	6.167778986615196E-5
24-25	2.9775484762969914E-5	8.039380886001877E-4	0.0	4.891686782487914E-5	6.805825088678837E-5
26-27	2.9775484762969914E-5	8.422208547240062E-4	0.0	8.08191729280612E-5	7.231189156721264E-5
28-29	3.4029125443394186E-5	8.443476750642184E-4	0.0	2.7010618320694136E-4	7.231189156721264E-5
30-31	3.4029125443394186E-5	9.421814107139766E-4	0.0	8.932645428890973E-4	7.231189156721264E-5
32-33	3.4029125443394186E-5	9.549423327552494E-4	0.0	0.0023607705776354715	8.507281360848546E-5
34-35	3.4029125443394186E-5	0.0011378488820134932	0.0	0.004185582429537485	1.0208737633018257E-4
36-37	3.4029125443394186E-5	0.001322882251611949	0.0	0.0074098420652990845	1.0208737633018257E-4
38-39	3.4029125443394186E-5	0.0013739259397770403	0.0	0.01907119799068223	1.0846783735081898E-4
40-41	3.4029125443394186E-5	0.0014972815195093442	0.0	0.03272113093416372	1.2335557973230392E-4
42-43	3.828276612381846E-5	0.0015738470517569813	0.0	0.03953971694488383	1.2548240007251608E-4
44-45	3.828276612381846E-5	0.001758880421355437	0.0	0.04769394612925716	1.276092204127282E-4
46-47	3.828276612381846E-5	0.001867348258706256	0.0	0.05700941921938632	1.3611650177357677E-4
48-49	3.828276612381846E-5	0.0018716018993866802	0.0	0.06575915809901905	1.6589198653654667E-4
50-51	3.828276612381846E-5	0.0020821571130676822	0.0	0.07603595398292409	1.680188068767588E-4
52-53	3.828276612381846E-5	0.002099171675789379	0.0	0.10872943625266507	1.8928701027888016E-4
54-55	3.828276612381846E-5	0.0021225666995317123	0.0	0.14934957793037668	2.084283933407894E-4
56-57	3.828276612381846E-5	0.002160849465655531	0.0	0.20270724662561876	2.084283933407894E-4
58-59	3.828276612381846E-5	0.002175737208037016	0.0	0.23781254316116027	2.1268203402121367E-4
60-61	3.828276612381846E-5	0.0022182736148412584	0.0	0.2537126520245862	2.1693567470163795E-4
62-63	3.828276612381846E-5	0.0022671904826661376	0.0	0.2663140625403431	2.211893153820622E-4
64-65	3.828276612381846E-5	0.002615989018460928	0.0	0.27060598598689123	2.211893153820622E-4
66-67	3.828276612381846E-5	0.0026308767608424133	0.0	0.27473414426724296	2.254429560624865E-4
68-69	3.828276612381846E-5	0.002741471418533444	0.0	0.27890058531371853	2.2969659674291077E-4
70-71	3.828276612381846E-5	0.0027882614660181115	0.0	0.28280755427868826	2.3395023742333503E-4
72-73	3.828276612381846E-5	0.0028052760287398085	0.0	0.2858510341855318	2.552184408254564E-4
74-75	3.828276612381846E-5	0.002879714740647233	0.0	0.2881224783088784	2.552184408254564E-4
76-77	3.828276612381846E-5	0.0030519871882044164	0.0	0.29048537570685407	2.552184408254564E-4
78-79	3.828276612381846E-5	0.003137060001812902	0.0	0.29196776948398195	2.552184408254564E-4
80-81	3.828276612381846E-5	0.0031498209238541744	0.0	0.2924867136469937	2.5947208150588067E-4
82-83	3.828276612381846E-5	0.003164708666235659	0.0	0.29290144361333503	2.7648664422757775E-4
84-85	3.828276612381846E-5	0.0031817232289573566	0.0	0.2931630425151811	2.7648664422757775E-4
86-87	3.828276612381846E-5	0.0032604155815452052	0.0	0.2932672567118515	2.8074028490800206E-4
88	3.828276612381846E-5	0.0034029125443394187	0.0	0.29327576399321237	2.849939255884263E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	34110	0.0	45.71104	1
GTATCAA	63405	0.0	33.85031	1
ATCAACG	85950	0.0	29.5558	3
TCAACGC	86620	0.0	29.391972	4
CAACGCA	87270	0.0	29.1137	5
TATCAAC	89135	0.0	28.733011	2
AACGCAG	90480	0.0	28.180649	6
ACGCAGA	101565	0.0	25.015709	7
CGCAGAG	103850	0.0	24.469446	8
TGTATCA	16140	0.0	23.348866	1
GCAGAGT	119355	0.0	21.348568	9
TTATCAA	21960	0.0	21.111885	1
TACATGG	82420	0.0	20.23239	2
GAGTACT	67680	0.0	20.090317	12-13
ACATGGG	80740	0.0	20.05932	3
AGAGTAC	106605	0.0	19.810246	10-11
CAGAGTA	115675	0.0	19.00699	10-11
CATGGGG	55360	0.0	18.643421	4
AGTACTT	72880	0.0	18.425879	12-13
GTACATG	79315	0.0	18.069155	1
>>END_MODULE