FastQCFastQC Report
Fri 27 May 2016
SRR2139295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39083605
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA493900.048.371351
GTATCAA886150.037.004311
ATCAACG1105200.029.1394183
TCAACGC1105250.029.1029854
CAACGCA1127450.028.5108095
TATCAAC1165200.027.8165782
AACGCAG1167600.027.6079756
ACGCAGA1349550.023.81237
CGCAGAG1369250.023.466558
GCAGAGT1617250.019.732049
GTACATG1135700.019.5323641
TACATGG1123600.019.1900352
AGAGTAC1449650.018.94188910-11
ACATGGG1120200.018.6977753
GAGTACT960900.018.25885612-13
CAGAGTA1571400.017.5607410-11
GTGGTAT233300.016.9131221
AGTACTT1053550.016.83530612-13
CATGGGG733350.015.9829024
GTACTTT1095000.015.98264714-15
Produced by FastQC (version 0.11.5)