##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139295_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39083605
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59703202915903	31.0	31.0	34.0	30.0	34.0
2	31.56595708098063	31.0	31.0	34.0	30.0	34.0
3	31.73147047208158	31.0	31.0	34.0	30.0	34.0
4	35.49293618129648	37.0	35.0	37.0	33.0	37.0
5	35.41214335269226	37.0	35.0	37.0	33.0	37.0
6	35.48296192226894	37.0	35.0	37.0	33.0	37.0
7	35.38292665172519	37.0	35.0	37.0	33.0	37.0
8	35.38538781159005	37.0	35.0	37.0	33.0	37.0
9	36.91313593001465	39.0	37.0	39.0	33.0	39.0
10-11	36.84181428504356	39.0	37.0	39.0	32.5	39.0
12-13	36.803653283774615	39.0	37.0	39.0	32.0	39.0
14-15	37.96382366723847	40.0	37.0	41.0	33.0	41.0
16-17	37.97589541701693	40.0	37.0	41.0	33.0	41.0
18-19	38.03568214856332	40.0	37.0	41.0	33.0	41.0
20-21	38.06080594715855	40.0	37.0	41.0	33.0	41.0
22-23	38.02413507663013	40.0	37.0	41.0	33.0	41.0
24-25	37.974550021552815	40.0	37.0	41.0	33.0	41.0
26-27	37.83907864106361	40.0	37.0	41.0	32.0	41.0
28-29	37.75697543476437	40.0	37.0	41.0	32.0	41.0
30-31	37.66744644791076	40.0	37.0	41.0	32.0	41.0
32-33	37.56260529917314	40.0	36.0	41.0	32.0	41.0
34-35	37.480001887323205	39.5	36.0	41.0	31.5	41.0
36-37	37.41804642076002	39.0	36.0	41.0	31.0	41.0
38-39	37.3562323862851	39.0	36.0	41.0	31.0	41.0
40-41	37.30392089285628	39.0	36.0	41.0	31.0	41.0
42-43	37.238451675973465	39.0	36.0	41.0	31.0	41.0
44-45	37.17844979678395	39.0	36.0	41.0	31.0	41.0
46-47	37.132284278480284	39.0	36.0	41.0	31.0	41.0
48-49	37.04725871619232	39.0	35.5	41.0	31.0	41.0
50-51	36.961706349639385	39.0	35.0	40.5	30.0	41.0
52-53	36.86383759404595	39.0	35.0	40.0	30.0	41.0
54-55	36.724027243488806	39.0	35.0	40.0	30.0	41.0
56-57	36.55684019749826	38.0	35.0	40.0	30.0	41.0
58-59	36.39282733773567	38.0	35.0	40.0	29.5	41.0
60-61	36.22320163455633	38.0	34.0	40.0	29.0	41.0
62-63	36.02476389759886	38.0	34.0	40.0	29.0	41.0
64-65	35.79638999761828	37.5	34.0	40.0	29.0	41.0
66-67	35.53003242644263	37.0	34.0	40.0	28.5	41.0
68-69	35.261283231437154	36.5	34.0	39.0	28.0	41.0
70-71	34.94937507866462	36.0	33.0	39.0	28.0	40.5
72-73	34.66131325679684	36.0	33.0	39.0	28.0	40.0
74-75	34.36749234005217	35.0	33.0	38.0	28.0	40.0
76-77	34.05265683957376	35.0	32.5	37.0	28.0	39.5
78-79	34.04661758828992	35.0	33.0	37.0	28.0	39.0
80-81	33.84558887051874	35.0	33.0	36.5	28.0	39.0
82-83	33.57862145330745	35.0	33.0	36.0	28.0	38.5
84-85	33.35144011002514	35.0	32.0	36.0	28.0	37.0
86-87	33.158513528253906	35.0	32.0	35.5	28.0	37.0
88-89	33.126400545808316	35.0	32.5	35.0	28.5	36.5
90-91	33.234428170080065	35.0	33.0	35.0	29.0	36.0
92-93	33.21686645403607	35.0	33.0	35.0	29.0	36.0
94-95	33.20414584878655	35.0	33.0	35.0	29.0	36.0
96-97	33.25091181440409	35.0	33.0	35.0	29.0	35.5
98-99	33.338124257155684	35.0	33.0	35.0	29.0	35.0
100	32.93784428302508	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	53.0
20	349.0
21	2515.0
22	11790.0
23	39123.0
24	100270.0
25	204859.0
26	351967.0
27	532353.0
28	731839.0
29	936969.0
30	1149614.0
31	1388309.0
32	1680395.0
33	2057123.0
34	2593189.0
35	3356997.0
36	4687382.0
37	7103352.0
38	8319738.0
39	3731379.0
40	104036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.59621432055718	21.28663156840317	14.188220354800945	24.92893375623871
2	14.079612154508265	22.56240436367116	40.7101264072237	22.64785707459688
3	17.21822487971619	27.615709963295348	31.232060092716623	23.934005064271837
4	12.10091546058763	19.53088513713103	39.651104344136115	28.717095058145226
5	12.152382053804914	38.95502218897157	36.3695314186089	12.523064338614617
6	27.941900446491562	40.25309845394251	19.3550032040289	12.449997895537015
7	24.715143344632615	33.51365873235081	24.64073362730997	17.130464295706602
8	23.640749106946508	37.39674474757383	21.832272125357935	17.130234020121737
9	25.258880290085827	16.419299089733403	21.542598232686057	36.779222387494706
10-11	23.168221048186318	27.235656741490455	30.105266645694527	19.4908555646287
12-13	24.355582091263077	24.95347345773247	29.942877582556676	20.74806686844778
14-15	21.55385487085953	26.117676452824657	27.4919304398865	24.836538236429316
16-17	20.961436387457095	29.19876377831574	28.08539667720007	21.754403157027095
18-19	21.04133306024355	28.44721591061009	29.773234838495576	20.738216190650785
20-21	21.964656650790086	27.587141657425086	29.580747439523737	20.867454252261094
22-23	21.590907113207845	27.5344151892742	29.494912877746287	21.379764819771665
24-25	21.36729031673435	27.690443703923716	29.52002523620766	21.42224074313427
26-27	21.430139773638327	27.78161383770328	29.382982058829477	21.405264329828917
28-29	21.41009287719232	27.81056069879731	29.401386615937895	21.37795980807248
30-31	21.68846861848847	27.668322762610977	29.3025742893446	21.340634329555954
32-33	21.50905154764448	27.83866009262514	29.238839011923627	21.413449347806754
34-35	21.60351608182934	27.849736069423052	29.221392751441382	21.325355097306225
36-37	21.628482214894486	27.762595148564472	29.17706553694877	21.431857099592268
38-39	21.52907239260023	27.847491758852343	29.205850589536585	21.417585259010842
40-41	21.754272245315303	27.7686079088253	29.031380077968684	21.445739767890718
42-43	21.63563652673262	27.894513489492905	28.826306056300012	21.643543927474465
44-45	21.737685127398308	28.00256907783123	28.62222695040616	21.6375188443643
46-47	21.839172624061867	27.981243989778054	28.326529000062976	21.8530543860971
48-49	21.820535797589184	28.083840214136064	28.274662452042122	21.820961536232637
50-51	21.793061686793862	28.216670036796625	28.118636039704565	21.871632236704947
52-53	21.934259005108398	28.120231549388343	28.039157134588628	21.906352310914635
54-55	21.904977581286918	28.006455727131268	28.175196522777014	21.913370168804807
56-57	21.899236055303113	28.067597720911667	28.143847971677825	21.889318252107394
58-59	21.886542067060923	28.118617799716972	28.108638916651884	21.88620121657022
60-61	21.91787278500301	28.113122395827332	28.119597084282677	21.84940773488698
62-63	21.88542366357779	28.180170620779077	28.070336924749867	21.864068790893267
64-65	21.83789943119323	28.178376789786018	28.035578227406532	21.94814555161421
66-67	21.917722390476904	28.23255959961331	27.859473689843007	21.99024432006678
68-69	21.873010756458044	28.18809647454343	27.93127504926393	22.007617719734593
70-71	21.888432860926958	27.94945013451987	28.03418010437343	22.127936900179744
72-73	21.957424371410607	27.980671753060793	27.9537080898571	22.1081957856715
74-75	21.830084348085034	28.173115183154124	27.925188407082974	22.07161206167787
76-77	21.967202828598055	28.110748399355913	27.84998973241076	22.072059039635274
78-79	21.91408591888631	28.13147261063611	27.81916329345545	22.13527817702213
80-81	21.907494912715723	28.143804005864503	27.826930986093434	22.121770095326347
82-83	21.978672340604124	28.08463943337008	27.937061815043723	21.999626410982074
84-85	22.035283799487075	28.14497675935786	27.85161877015306	21.96812067100201
86-87	21.853986396805947	28.206522968654557	27.950491280614344	21.988999353925152
88-89	21.933425556541287	28.172366157875707	27.89073023167303	22.003478053909976
90-91	21.981109910639038	28.160076545847843	27.963162230827987	21.895651312685132
92-93	22.002489092789954	28.124959058843025	27.92846417734392	21.944087671023105
94-95	21.772730002665234	28.190398796078377	28.02906910282773	22.007802098428655
96-97	21.791368110942013	28.35378686865848	28.107679598684264	21.747165421715238
98-99	22.825381247086302	30.26089921532759	28.344820981802897	18.568898555783214
100	21.876345389778816	31.255101084634408	22.155278580594327	24.713274944992452
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5695.0
1	6075.0
2	11515.5
3	16796.0
4	21049.5
5	35779.0
6	48917.0
7	56932.5
8	60851.5
9	56217.5
10	46378.5
11	35456.0
12	35429.0
13	50438.5
14	92731.0
15	155160.0
16	205696.0
17	231305.0
18	234876.5
19	222987.0
20	201855.5
21	180671.5
22	170802.0
23	182751.5
24	220138.0
25	280306.0
26	361111.5
27	451251.5
28	539155.5
29	633732.0
30	725915.0
31	816545.5
32	915057.5
33	1006471.0
34	1095759.5
35	1180473.5
36	1268812.5
37	1354477.0
38	1410161.0
39	1449755.5
40	1494531.5
41	1531156.0
42	1552561.5
43	1573170.0
44	1587929.0
45	1599572.0
46	1598615.5
47	1587575.5
48	1568905.0
49	1526074.5
50	1475015.0
51	1414220.0
52	1335229.5
53	1246153.5
54	1152878.0
55	1050853.0
56	949148.0
57	850716.0
58	750482.5
59	651391.5
60	543100.5
61	436623.5
62	342550.0
63	262472.0
64	196653.0
65	144548.0
66	107881.0
67	82169.0
68	61704.0
69	44360.0
70	31580.0
71	22647.0
72	15960.0
73	10906.5
74	7411.0
75	5063.5
76	3475.5
77	2433.5
78	1752.0
79	1324.0
80	1013.5
81	757.0
82	584.5
83	425.0
84	308.0
85	228.0
86	160.0
87	121.5
88	82.5
89	49.5
90	34.5
91	26.5
92	20.5
93	10.0
94	4.5
95	4.0
96	3.0
97	2.5
98	1.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.700136587241061E-4
24-25	1.4977031378288834E-4
26-27	1.536783705595067E-4
28-29	4.446710031102494E-4
30-31	4.182410147974184E-4
32-33	4.922756424839791E-4
34-35	9.160459124272735E-4
36-37	4.7046082464958296E-4
38-39	0.0017989636801044363
40-41	7.697421911611678E-5
42-43	1.4434331664199708E-4
44-45	9.779401933802727E-5
46-47	3.731296841267994E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.3855710812028205E-6
60-61	0.0
62-63	2.5306972878039148E-5
64-65	0.0
66-67	0.0
68-69	3.9850350281701695E-4
70-71	0.0
72-73	3.658954098801362E-4
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	1.2499028200557405E-5
88-89	0.0
90-91	0.0
92-93	4.194300195425239E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1772.0
20-21	6977.0
22-23	11902.0
24-25	15488.0
26-27	21914.0
28-29	36688.0
30-31	68079.0
32-33	86262.0
34-35	114739.0
36-37	153823.0
38-39	189560.0
40-41	213837.0
42-43	253017.0
44-45	310575.0
46-47	301565.0
48-49	264847.0
50-51	224888.0
52-53	199739.0
54-55	213815.0
56-57	244966.0
58-59	256544.0
60-61	265512.0
62-63	254427.0
64-65	261583.0
66-67	283905.0
68-69	293037.0
70-71	300204.0
72-73	284378.0
74-75	281617.0
76-77	292433.0
78-79	316807.0
80-81	334118.0
82-83	322862.0
84-85	317304.0
86-87	334278.0
88-89	360226.0
90-91	407645.0
92-93	443242.0
94-95	550497.0
96-97	1391863.0
98-99	2547063.0
100-101	2.6049607E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.10978389592249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.65269001126869	66.04660416873367
2	8.908687245648931	13.738938966167
3	2.619084883308315	6.058712080709445
4	1.1283180538721893	3.480174451998093
5	0.5833631888005476	2.2491504710623227
6	0.3499673109812747	1.6191542232241916
7	0.20954194434615817	1.131041383196457
8	0.13221522368438038	0.8156069860842898
9	0.09337039190483529	0.6479793667853496
>10	0.3156887293802498	3.6363118694454992
>50	0.005171083319358218	0.26861468920726456
>100	0.0018207443246556067	0.23790923599078723
>500	5.721064163288218E-5	0.028842477042814604
>1k	2.397851870191325E-5	0.04095963035286619
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	2.55861760960894E-6	0.0	2.55861760960894E-6	2.55861760960894E-6
2	0.0	2.3027558486480456E-5	0.0	1.023447043843576E-5	5.11723521921788E-6
3	0.0	2.8144793705698337E-5	0.0	2.5586176096089396E-5	5.11723521921788E-6
4	0.0	8.443438111709501E-5	0.0	2.8144793705698337E-5	5.11723521921788E-6
5	5.11723521921788E-6	8.955161633631289E-5	0.0	3.0703411315307274E-5	5.11723521921788E-6
6	5.11723521921788E-6	8.955161633631289E-5	0.0	3.0703411315307274E-5	1.023447043843576E-5
7	5.11723521921788E-6	8.955161633631289E-5	0.0	3.5820646534525156E-5	1.023447043843576E-5
8	5.11723521921788E-6	9.466885155553077E-5	0.0	3.8379264144134096E-5	1.2793088048044698E-5
9	5.11723521921788E-6	1.7910323267262578E-4	0.0	5.884820502100561E-5	1.5351705657653637E-5
10-11	5.11723521921788E-6	1.7910323267262578E-4	0.0	8.699299872670394E-5	1.5351705657653637E-5
12-13	5.11723521921788E-6	1.816618502822347E-4	0.0	1.0362401318916205E-4	1.5351705657653637E-5
14-15	6.396544024022349E-6	2.4818590813206715E-4	0.0	1.0746193960357547E-4	2.046894087687152E-5
16-17	1.151377924324023E-5	2.5969968737530734E-4	0.0	1.2793088048044696E-4	3.5820646534525156E-5
18-19	1.2793088048044698E-5	2.96799642714637E-4	0.0	1.5095843896692742E-4	3.709995533932962E-5
20-21	1.6631014462458107E-5	3.2494443642033533E-4	0.0	1.6631014462458105E-4	3.8379264144134096E-5
22-23	1.7910323267262578E-5	3.81234023831732E-4	0.0	1.957342471350839E-4	4.093788175374304E-5
24-25	2.046894087687152E-5	4.2217190558547504E-4	0.0	2.4051005530324033E-4	4.605511697296091E-5
26-27	2.046894087687152E-5	4.413615376575421E-4	0.0	2.955203339098325E-4	4.605511697296091E-5
28-29	2.046894087687152E-5	4.4775808168156443E-4	0.0	6.12788917501341E-4	4.989304338737432E-5
30-31	2.046894087687152E-5	4.963718162641342E-4	0.0	0.0018754667078433528	5.2451660996983266E-5
32-33	2.046894087687152E-5	5.040476690929611E-4	0.0	0.004723208107338103	5.373096980178773E-5
34-35	2.046894087687152E-5	5.897613590148607E-4	0.0	0.008353886495373188	5.6289587411396674E-5
36-37	2.046894087687152E-5	6.844302105703914E-4	0.0	0.014836144209317437	5.6289587411396674E-5
38-39	2.046894087687152E-5	7.087370778616763E-4	0.0	0.03765389605180996	6.140682263061455E-5
40-41	2.046894087687152E-5	7.547921948346371E-4	0.0	0.06380040940440371	6.39654402402235E-5
42-43	2.046894087687152E-5	7.803783709307266E-4	0.0	0.07773975814155322	6.524474904502796E-5
44-45	2.046894087687152E-5	8.712092960718439E-4	0.0	0.09527780254661769	6.652405784983243E-5
46-47	2.046894087687152E-5	9.569229859937434E-4	0.0	0.1122491131511538	6.908267545944136E-5
48-49	2.3027558486480456E-5	9.709953828465926E-4	0.0	0.12803194587602654	7.419991067865924E-5
50-51	2.3027558486480456E-5	0.0010733400872309502	0.0	0.14430603318194418	7.547921948346373E-5
52-53	2.3027558486480456E-5	0.0010848538664741903	0.0	0.19918838090805596	9.594816036033524E-5
54-55	2.5586176096089396E-5	0.0010989262633270395	0.0	0.26487321218193666	1.1641710123720675E-4
56-57	2.5586176096089396E-5	0.0011283503658375423	0.0	0.3246527540128399	1.2025502765162016E-4
58-59	2.5586176096089396E-5	0.001148819306714414	0.0	0.3461106005958253	1.2793088048044696E-4
60-61	2.5586176096089396E-5	0.0011884778796633524	0.0	0.3600141286864402	1.356067333092738E-4
62-63	2.5586176096089396E-5	0.0012127847469546372	0.0	0.36996715118781903	1.4328258613810062E-4
64-65	2.5586176096089396E-5	0.0013458328626543023	0.0	0.3745189319153133	1.4456189494290508E-4
66-67	2.8144793705698337E-5	0.0013522294066783246	0.0	0.3792024814497025	1.4584120374770956E-4
68-69	2.8144793705698337E-5	0.0013867707444080452	0.0	0.38391673439540697	1.4584120374770956E-4
70-71	2.8144793705698337E-5	0.0014059603764801122	0.0	0.3878889882343248	1.5479636538134087E-4
72-73	2.9424102510502807E-5	0.0014097983028945257	0.0	0.39105911545263033	1.9701355593988834E-4
74-75	3.198272012011174E-5	0.0014609706550867045	0.0	0.39343990913837146	1.9701355593988834E-4
76-77	3.3262028924916215E-5	0.0015441257273989951	0.0	0.39581814420650296	1.9701355593988834E-4
78-79	3.3262028924916215E-5	0.0015837843003479336	0.0	0.39727783555278484	1.9701355593988834E-4
80-81	3.3262028924916215E-5	0.0015863429179575426	0.0	0.39779339700112104	2.0085148235430176E-4
82-83	3.3262028924916215E-5	0.0015978566972007827	0.0	0.39812857590797984	2.0852733518312856E-4
84-85	3.3262028924916215E-5	0.0016119290940536319	0.0	0.3982833722733612	2.0980664398793304E-4
86-87	3.3262028924916215E-5	0.0016541462846121793	0.0	0.39835117564001576	2.0980664398793304E-4
88	3.3262028924916215E-5	0.0017526530625821236	0.0	0.39836141011045423	2.12365261597542E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	49390	0.0	48.37135	1
GTATCAA	88615	0.0	37.00431	1
ATCAACG	110520	0.0	29.139418	3
TCAACGC	110525	0.0	29.102985	4
CAACGCA	112745	0.0	28.510809	5
TATCAAC	116520	0.0	27.816578	2
AACGCAG	116760	0.0	27.607975	6
ACGCAGA	134955	0.0	23.8123	7
CGCAGAG	136925	0.0	23.46655	8
GCAGAGT	161725	0.0	19.73204	9
GTACATG	113570	0.0	19.532364	1
TACATGG	112360	0.0	19.190035	2
AGAGTAC	144965	0.0	18.941889	10-11
ACATGGG	112020	0.0	18.697775	3
GAGTACT	96090	0.0	18.258856	12-13
CAGAGTA	157140	0.0	17.56074	10-11
GTGGTAT	23330	0.0	16.913122	1
AGTACTT	105355	0.0	16.835306	12-13
CATGGGG	73335	0.0	15.982902	4
GTACTTT	109500	0.0	15.982647	14-15
>>END_MODULE