FastQCFastQC Report
Fri 27 May 2016
SRR2139294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25956927
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA313500.048.2941361
GTATCAA559100.037.8525541
ATCAACG696750.029.6032263
TCAACGC699850.029.4535224
CAACGCA712350.028.9245195
TATCAAC741800.028.0565852
AACGCAG738850.027.9985286
ACGCAGA852200.024.16777
CGCAGAG867300.023.7519288
GTACATG755850.020.311621
TACATGG744400.020.1001472
GCAGAGT1026300.019.877939
AGACCGA185750.019.72557688-89
ACATGGG746450.019.6095643
CGAGACC187800.019.46379986-87
AGAGTAC917750.019.25920510-11
GAGACCG187750.019.1345288-89
GACCGAG190400.019.11011790-91
ACCGAGG198800.018.52878290-91
ACGAGAC197950.018.3836586-87
Produced by FastQC (version 0.11.5)