##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139294_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25956927
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.572949871916656	31.0	31.0	34.0	30.0	34.0
2	31.541025792459948	31.0	31.0	34.0	30.0	34.0
3	31.708513646472866	31.0	31.0	34.0	30.0	34.0
4	35.47386221797365	37.0	35.0	37.0	33.0	37.0
5	35.38890420272014	37.0	35.0	37.0	33.0	37.0
6	35.45948312756745	37.0	35.0	37.0	33.0	37.0
7	35.356102014695345	37.0	35.0	37.0	32.0	37.0
8	35.3575013328812	37.0	35.0	37.0	32.0	37.0
9	36.881599928990056	39.0	37.0	39.0	33.0	39.0
10-11	36.80757462160293	39.0	37.0	39.0	32.0	39.0
12-13	36.76732266881977	39.0	37.0	39.0	32.0	39.0
14-15	37.91939748877053	40.0	37.0	41.0	33.0	41.0
16-17	37.93150315906039	40.0	37.0	41.0	33.0	41.0
18-19	37.991724290013224	40.0	37.0	41.0	33.0	41.0
20-21	38.013029668388064	40.0	37.0	41.0	33.0	41.0
22-23	37.97757104567098	40.0	37.0	41.0	33.0	41.0
24-25	37.928054514321104	40.0	37.0	41.0	32.0	41.0
26-27	37.78955086505498	40.0	37.0	41.0	32.0	41.0
28-29	37.70807888277735	40.0	37.0	41.0	32.0	41.0
30-31	37.61752204965355	40.0	36.5	41.0	32.0	41.0
32-33	37.51245565552837	39.0	36.0	41.0	32.0	41.0
34-35	37.42708728248223	39.0	36.0	41.0	31.0	41.0
36-37	37.365627260629225	39.0	36.0	41.0	31.0	41.0
38-39	37.30044907470195	39.0	36.0	41.0	31.0	41.0
40-41	37.24671061615098	39.0	36.0	41.0	31.0	41.0
42-43	37.17948068777973	39.0	36.0	41.0	31.0	41.0
44-45	37.115720776603894	39.0	36.0	41.0	31.0	41.0
46-47	37.06888409927035	39.0	36.0	41.0	31.0	41.0
48-49	36.98048690451099	39.0	35.0	41.0	30.0	41.0
50-51	36.89349600664407	39.0	35.0	40.0	30.0	41.0
52-53	36.79499335857074	39.0	35.0	40.0	30.0	41.0
54-55	36.649954854471986	39.0	35.0	40.0	30.0	41.0
56-57	36.47921018574041	38.0	35.0	40.0	30.0	41.0
58-59	36.31309477534004	38.0	34.5	40.0	29.0	41.0
60-61	36.14122285770276	38.0	34.0	40.0	29.0	41.0
62-63	35.936139765806494	38.0	34.0	40.0	29.0	41.0
64-65	35.70680849211054	37.0	34.0	40.0	29.0	41.0
66-67	35.44136399202729	37.0	34.0	40.0	28.0	41.0
68-69	35.170389322925544	36.0	33.5	39.0	28.0	41.0
70-71	34.85862746118732	36.0	33.0	39.0	28.0	40.5
72-73	34.56695429939923	35.5	33.0	39.0	28.0	40.0
74-75	34.27552724062871	35.0	33.0	38.0	28.0	40.0
76-77	33.96310013887313	35.0	32.0	37.0	27.5	39.0
78-79	33.96152763970012	35.0	33.0	37.0	28.0	39.0
80-81	33.757219087038244	35.0	33.0	36.5	28.0	39.0
82-83	33.49295660387021	35.0	32.0	36.0	28.0	38.0
84-85	33.2766578320211	35.0	32.0	36.0	28.0	37.0
86-87	33.08779431403066	35.0	32.0	35.0	27.5	37.0
88-89	33.05807580406461	35.0	32.0	35.0	28.0	36.5
90-91	33.172488878558035	35.0	33.0	35.0	29.0	36.0
92-93	33.16447451607565	35.0	33.0	35.0	29.0	36.0
94-95	33.15234626963872	35.0	33.0	35.0	29.0	36.0
96-97	33.20339392000192	35.0	33.0	35.0	29.0	35.5
98-99	33.29823731473399	35.0	33.0	35.0	29.0	35.0
100	32.893032493996714	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	4.0
19	41.0
20	257.0
21	1748.0
22	8142.0
23	27303.0
24	69334.0
25	142337.0
26	242770.0
27	367408.0
28	503245.0
29	640866.0
30	787626.0
31	946696.0
32	1142731.0
33	1394651.0
34	1753587.0
35	2256340.0
36	3143156.0
37	4743836.0
38	5420636.0
39	2307558.0
40	56654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.56572363130659	21.24593947503878	14.173858099612485	25.014478794042144
2	14.084506228337428	22.495405561683015	40.647373242603024	22.77271496737653
3	17.25217318675666	27.540810204536154	31.131820804519734	24.07519580418745
4	12.122109061677447	19.4463042562781	39.56420187952141	28.867384802523045
5	12.225056533078819	38.941408588158374	36.25810173908491	12.575433139677896
6	28.085689804498042	40.10559878679013	19.33076669668948	12.477944712022344
7	24.81709025109174	33.44578115891762	24.524929318482116	17.212199271508528
8	23.741381250561748	37.30097172134436	21.784990187783016	17.172656840310875
9	25.310130124417267	16.352459595852775	21.427890905575993	36.90951937415396
10-11	23.26925294353989	27.182609096985942	30.024259034977447	19.523878924496724
12-13	24.425085064961657	24.84147873128433	29.829118446879328	20.904317756874686
14-15	21.60283649909714	26.011004692504624	27.394664245116534	24.9914945632817
16-17	21.078458170337345	29.070979781235273	27.994719868033684	21.855842180393694
18-19	21.1425971186805	28.345267912492105	29.605571183368507	20.906563785458886
20-21	22.063472851306997	27.56230238937133	29.344707951075254	21.02951680824642
22-23	21.701597166946346	27.49711794711127	29.268179188061684	21.5331056978807
24-25	21.474267287037048	27.61886871978008	29.335627374096855	21.571236619086015
26-27	21.53041365401631	27.71913676229402	29.178766244864768	21.571683338824904
28-29	21.52558766066239	27.75186365853177	29.207476347796334	21.515072333009506
30-31	21.84314939006777	27.579715368114222	29.08840578766786	21.48872945415015
32-33	21.65856077927656	27.789035605363583	29.033442993880566	21.518960621479295
34-35	21.693507378766235	27.779673081420043	29.06919424265596	21.45762529715777
36-37	21.73314625829106	27.685023366293564	29.01880431680639	21.56302605860899
38-39	21.610864506155394	27.80190982253717	29.040782703336337	21.546442967971096
40-41	21.851853537442626	27.698409992541308	28.882428382731874	21.567308087284196
42-43	21.74649337998374	27.782050291383587	28.714409531059452	21.757046797573224
44-45	21.8335521145831	27.923799921100972	28.49403013167831	21.748617832637613
46-47	21.9492768746992	27.8965285248007	28.193063835828365	21.961130764671736
48-49	21.90549615855877	27.98889068266738	28.17479563705154	21.930817521722314
50-51	21.870066531691762	28.104694637530393	28.02759277820968	21.997646052568165
52-53	21.996519461712854	28.01297784698194	27.95750720139499	22.032995489910213
54-55	21.99100586903072	27.91023333760727	28.070420989161722	22.02833980420029
56-57	21.977366451712015	27.964949430483493	28.052853750671602	22.00483036713289
58-59	21.938573120944035	27.96179401490767	28.004483748287907	22.095149115860394
60-61	22.07498169283373	27.943985646473013	28.078408756843633	21.902623903849626
62-63	21.9324226378561	28.027588652135414	28.015282334691662	22.02470637531682
64-65	21.855199552632936	28.017537587027526	28.01338880235196	22.11387405798758
66-67	21.943935360610407	28.089162362749086	27.980923056458295	21.985979220182212
68-69	21.88073079805186	28.151653129606245	27.902212233294488	22.065403839047406
70-71	21.944515756734468	27.853464008461838	27.857172608161452	22.344847626642242
72-73	21.976226745291193	27.870428456898505	27.826572643293307	22.32677215451699
74-75	21.848041447892506	28.093091253210417	27.93552524638055	22.12334205251653
76-77	22.002582680297202	27.912100432875704	27.828619700814105	22.25669718601299
78-79	21.941266431662605	27.942235884937034	27.668972641241226	22.44752504215914
80-81	22.1003348502396	28.10394709958742	27.641217976197378	22.1545000739756
82-83	22.13512404044827	27.89797621816888	27.799920636006625	22.166979105376228
84-85	22.09842241790491	27.900649617008316	27.713988173349772	22.286939791737005
86-87	21.909406800233267	28.093971738396473	27.778337638082945	22.218283823287315
88-89	22.10331146080497	28.085517161721963	27.727408049822554	22.083763327650512
90-91	22.188963763207	28.046852307436325	27.816467596058995	21.947716333297677
92-93	22.023150246598462	27.881030631772784	27.788838595658	22.30698052597075
94-95	21.972472222557045	28.10941263801131	27.90001536158664	22.018099777845002
96-97	22.135314878712105	28.124372685955024	27.9790437614028	21.761268673930072
98-99	22.956239049701637	30.17859004579742	28.2418376431406	18.62333326136034
100	22.01035709655002	31.193999638006396	21.997666121002464	24.797977144441123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3775.0
1	3933.0
2	7132.5
3	10369.5
4	13030.0
5	22146.5
6	30397.5
7	35729.5
8	38547.0
9	36028.5
10	30021.5
11	23209.0
12	23097.5
13	31879.5
14	57678.5
15	96102.0
16	127121.0
17	142945.0
18	145155.0
19	137334.5
20	123758.0
21	110655.0
22	105100.0
23	113372.5
24	137161.5
25	176320.5
26	230677.0
27	291085.0
28	349007.5
29	411504.0
30	471160.0
31	527075.5
32	589788.5
33	650174.0
34	709324.0
35	764899.0
36	824126.0
37	880624.5
38	911675.0
39	932488.5
40	962715.0
41	989640.5
42	1007970.5
43	1025678.5
44	1038692.0
45	1052357.5
46	1059068.0
47	1056944.5
48	1047839.5
49	1025454.5
50	996852.0
51	957816.5
52	906008.5
53	847423.0
54	786471.5
55	721730.5
56	655983.0
57	587641.5
58	518373.5
59	450010.5
60	373537.0
61	299077.5
62	233570.0
63	177102.5
64	130692.5
65	94888.5
66	69240.0
67	51921.5
68	39330.0
69	28515.0
70	20273.0
71	14125.0
72	9527.5
73	6366.5
74	4326.5
75	2984.0
76	2075.0
77	1458.0
78	1088.5
79	833.0
80	608.5
81	435.5
82	331.5
83	236.5
84	172.0
85	130.0
86	98.5
87	68.5
88	45.5
89	35.5
90	27.0
91	21.0
92	15.5
93	7.5
94	2.5
95	2.5
96	2.5
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.678283661030331E-4
24-25	1.8310249601660519E-4
26-27	2.0246265157004872E-4
28-29	4.3797594913922295E-4
30-31	5.157096955392888E-4
32-33	4.7596672760395157E-4
34-35	0.0010277389256913149
36-37	4.4146963491141874E-4
38-39	0.0019777466214894414
40-41	1.1181557319830929E-4
42-43	1.913062133832265E-4
44-45	1.330136347313768E-4
46-47	2.8009183651135534E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.072317111435309E-6
60-61	0.0
62-63	2.308877036093709E-5
64-65	0.0
66-67	0.0
68-69	4.913946918644137E-4
70-71	0.0
72-73	4.494655222198081E-4
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	2.3252157957210495E-6
88-89	0.0
90-91	0.0
92-93	4.4073952431392577E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1163.0
20-21	4544.0
22-23	7520.0
24-25	9803.0
26-27	14269.0
28-29	23002.0
30-31	42167.0
32-33	53481.0
34-35	70447.0
36-37	94057.0
38-39	116228.0
40-41	131666.0
42-43	157347.0
44-45	191089.0
46-47	186852.0
48-49	164959.0
50-51	140034.0
52-53	124239.0
54-55	132239.0
56-57	155544.0
58-59	102562.0
60-61	171587.0
62-63	163731.0
64-65	168454.0
66-67	182626.0
68-69	189349.0
70-71	195484.0
72-73	185036.0
74-75	183234.0
76-77	190441.0
78-79	207127.0
80-81	218758.0
82-83	211957.0
84-85	208825.0
86-87	219284.0
88-89	236659.0
90-91	268823.0
92-93	296721.0
94-95	368512.0
96-97	1000242.0
98-99	1713518.0
100-101	1.7453347E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.00141054572484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.22545347567551	68.11932449523137
2	8.782290205422537	13.87626628100568
3	2.6386328454031793	6.25367150097392
4	1.0332664349840153	3.2651802333315874
5	0.48405114714287023	1.9120361700281479
6	0.26776682177943256	1.2692373970752533
7	0.1713859197907682	0.9477810587803002
8	0.10708008957193871	0.6767582494036575
9	0.0711802926935833	0.5061009173265568
>10	0.21177893522874072	2.541908910829548
>50	0.005064076567575017	0.26748450351214176
>100	0.0019543546427533806	0.24858910079412017
>500	5.306936712198474E-5	0.03246454390847714
>1k	4.233173001445782E-5	0.08319663779916188
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1557608495027165E-5
2	0.0	7.70507233001811E-6	0.0	0.0	1.1557608495027165E-5
3	3.852536165009055E-6	7.70507233001811E-6	0.0	3.852536165009055E-6	1.1557608495027165E-5
4	3.852536165009055E-6	2.311521699005433E-5	0.0	7.70507233001811E-6	1.1557608495027165E-5
5	3.852536165009055E-6	2.311521699005433E-5	0.0	7.70507233001811E-6	1.1557608495027165E-5
6	3.852536165009055E-6	2.311521699005433E-5	0.0	7.70507233001811E-6	1.9262680825045276E-5
7	3.852536165009055E-6	2.311521699005433E-5	0.0	7.70507233001811E-6	1.9262680825045276E-5
8	1.541014466003622E-5	2.311521699005433E-5	0.0	1.541014466003622E-5	2.311521699005433E-5
9	1.9262680825045276E-5	7.319818713517205E-5	0.0	1.9262680825045276E-5	3.082028932007244E-5
10-11	2.6967753155063385E-5	7.319818713517205E-5	0.0	2.311521699005433E-5	3.082028932007244E-5
12-13	2.6967753155063385E-5	7.319818713517205E-5	0.0	2.6967753155063385E-5	3.46728254850815E-5
14-15	2.6967753155063385E-5	1.0401847645524449E-4	0.0	3.082028932007244E-5	5.200923822762225E-5
16-17	2.6967753155063385E-5	1.0401847645524449E-4	0.0	3.6599093567586025E-5	7.319818713517205E-5
18-19	2.6967753155063385E-5	1.1557608495027166E-4	0.0	4.4304165897604134E-5	9.05345998777128E-5
20-21	2.8894021237567916E-5	1.2905996152780334E-4	0.0	6.356684672264941E-5	9.631340412522638E-5
22-23	3.082028932007244E-5	1.5602771468286675E-4	0.0	9.438713604272185E-5	1.0401847645524449E-4
24-25	3.082028932007244E-5	1.7914293167292108E-4	0.0	1.3291249769281242E-4	1.3098622961030788E-4
26-27	3.082028932007244E-5	1.887742720854437E-4	0.0	1.9840561249796632E-4	1.3098622961030788E-4
28-29	3.082028932007244E-5	1.907005401679482E-4	0.0	4.52672999388564E-4	1.3098622961030788E-4
30-31	3.082028932007244E-5	2.234470975705252E-4	0.0	0.0016373278701288486	1.3098622961030788E-4
32-33	3.082028932007244E-5	2.234470975705252E-4	0.0	0.004214674564519906	1.4832264235284864E-4
34-35	3.082028932007244E-5	2.6775126346812936E-4	0.0	0.007439247334632486	1.6180651893038032E-4
36-37	3.082028932007244E-5	3.082028932007244E-4	0.0	0.013466540164789154	1.6180651893038032E-4
38-39	3.46728254850815E-5	3.274655740257697E-4	0.0	0.0371423011668523	1.7143785934290297E-4
40-41	3.46728254850815E-5	3.4287571868580593E-4	0.0	0.06469756608707956	1.810691997554256E-4
42-43	3.46728254850815E-5	3.544333271808331E-4	0.0	0.07880362725526022	1.8684800400293919E-4
44-45	3.6599093567586025E-5	4.2185271006849154E-4	0.0	0.09679689741393502	1.945530763329573E-4
46-47	3.852536165009055E-5	4.661568759660957E-4	0.0	0.11420458207552844	1.9840561249796632E-4
48-49	3.852536165009055E-5	4.680831440486002E-4	0.0	0.13068187925327215	2.234470975705252E-4
50-51	3.852536165009055E-5	5.297237226887451E-4	0.0	0.14751168349011423	2.234470975705252E-4
52-53	3.852536165009055E-5	5.316499907712496E-4	0.0	0.20789826160854863	2.44636046478075E-4
54-55	3.852536165009055E-5	5.335762588537542E-4	0.0	0.2812293612414135	2.735300677156429E-4
56-57	3.852536165009055E-5	5.393550631012677E-4	0.0	0.34682071571877515	2.831614081281656E-4
58-59	3.852536165009055E-5	5.412813311837723E-4	0.0	0.49111938404727185	2.870139442931746E-4
60-61	3.852536165009055E-5	5.489864035137903E-4	0.0	0.6223637335806353	2.985715527882018E-4
62-63	3.852536165009055E-5	5.624702800913221E-4	0.0	0.6288571832867581	3.1205542936573344E-4
64-65	3.852536165009055E-5	6.356684672264941E-4	0.0	0.6343181533006584	3.159079655307425E-4
66-67	3.852536165009055E-5	6.452998076390168E-4	0.0	0.6390914456091046	3.236130378607607E-4
68-69	3.852536165009055E-5	6.60709952299053E-4	0.0	0.644128636644854	3.3324437827328326E-4
70-71	3.852536165009055E-5	6.741938288765846E-4	0.0	0.6480601498012457	3.5443332718083307E-4
72-73	3.852536165009055E-5	6.838251692891074E-4	0.0	0.6511132847120153	3.929586888309236E-4
74-75	3.852536165009055E-5	6.97309045866639E-4	0.0	0.6533708709047107	3.929586888309236E-4
76-77	3.852536165009055E-5	7.300556032692159E-4	0.0	0.6556188257569935	3.968112249959327E-4
78-79	3.852536165009055E-5	7.396869436817386E-4	0.0	0.6571656190272446	3.968112249959327E-4
80-81	3.852536165009055E-5	7.435394798467476E-4	0.0	0.6577820248136461	4.122213696559689E-4
82-83	3.852536165009055E-5	7.473920160117567E-4	0.0	0.6582154351322096	4.411153908935368E-4
84-85	3.852536165009055E-5	7.685809649193065E-4	0.0	0.6583406425575724	4.4304165897604135E-4
86-87	3.852536165009055E-5	7.955487180743699E-4	0.0	0.6584369559616976	4.4304165897604135E-4
88	3.852536165009055E-5	8.39852883971974E-4	0.0	0.6584523661063577	4.468941951410504E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	31350	0.0	48.294136	1
GTATCAA	55910	0.0	37.852554	1
ATCAACG	69675	0.0	29.603226	3
TCAACGC	69985	0.0	29.453522	4
CAACGCA	71235	0.0	28.924519	5
TATCAAC	74180	0.0	28.056585	2
AACGCAG	73885	0.0	27.998528	6
ACGCAGA	85220	0.0	24.1677	7
CGCAGAG	86730	0.0	23.751928	8
GTACATG	75585	0.0	20.31162	1
TACATGG	74440	0.0	20.100147	2
GCAGAGT	102630	0.0	19.87793	9
AGACCGA	18575	0.0	19.725576	88-89
ACATGGG	74645	0.0	19.609564	3
CGAGACC	18780	0.0	19.463799	86-87
AGAGTAC	91775	0.0	19.259205	10-11
GAGACCG	18775	0.0	19.13452	88-89
GACCGAG	19040	0.0	19.110117	90-91
ACCGAGG	19880	0.0	18.528782	90-91
ACGAGAC	19795	0.0	18.38365	86-87
>>END_MODULE