FastQCFastQC Report
Fri 27 May 2016
SRR2139293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17313138
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232850.050.408321
ATCTCGT105950.041.68552894
GTATCAA419550.037.0159761
TATCTCG135400.035.29714694
ATCAACG519450.029.3624843
TCAACGC520700.029.2669984
CAACGCA526600.028.9720525
TATCAAC544300.028.2531342
AACGCAG544750.028.0704866
TATGCCG58300.026.71219686-87
GCCGTCT63550.026.40015490-91
ATGCCGT63350.026.3179688-89
TCTCGTA61800.025.1562382-83
TGCCGTC62450.024.8438888-89
ACGCAGA627450.024.2877147
CTCGTAT61900.024.1331882-83
CCGTCTT65800.024.12842690-91
TCGTATG68450.024.08485284-85
CGCAGAG633500.024.0420638
CGTATGC68150.022.73785284-85
Produced by FastQC (version 0.11.5)