##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139293_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17313138
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66017206124043	31.0	31.0	34.0	30.0	34.0
2	31.62890776934834	31.0	31.0	34.0	30.0	34.0
3	31.79581795050672	31.0	31.0	34.0	30.0	34.0
4	35.54846983833895	37.0	35.0	37.0	33.0	37.0
5	35.469119174120834	37.0	35.0	37.0	33.0	37.0
6	35.536603243155575	37.0	35.0	37.0	33.0	37.0
7	35.43722657325321	37.0	35.0	37.0	33.0	37.0
8	35.437342092461805	37.0	35.0	37.0	33.0	37.0
9	36.97889019310075	39.0	37.0	39.0	33.0	39.0
10-11	36.90879281964945	39.0	37.0	39.0	33.0	39.0
12-13	36.871680483341606	39.0	37.0	39.0	32.5	39.0
14-15	38.043366546261	40.0	37.0	41.0	33.0	41.0
16-17	38.05616763408228	40.0	37.0	41.0	33.0	41.0
18-19	38.119184892998604	40.0	37.0	41.0	33.0	41.0
20-21	38.14391165149651	40.0	37.0	41.0	33.0	41.0
22-23	38.10905425176203	40.0	37.0	41.0	33.0	41.0
24-25	38.060986405551674	40.0	37.0	41.0	33.0	41.0
26-27	37.929060643472894	40.0	37.0	41.0	33.0	41.0
28-29	37.85025223791537	40.0	37.0	41.0	32.0	41.0
30-31	37.760693045140115	40.0	37.0	41.0	32.0	41.0
32-33	37.65790347946708	40.0	36.5	41.0	32.0	41.0
34-35	37.576654121835276	40.0	36.0	41.0	32.0	41.0
36-37	37.517459965822546	40.0	36.0	41.0	31.5	41.0
38-39	37.45589126116354	39.5	36.0	41.0	31.0	41.0
40-41	37.404747993900244	39.0	36.0	41.0	31.0	41.0
42-43	37.341037016723675	39.0	36.0	41.0	31.0	41.0
44-45	37.2833813429839	39.0	36.0	41.0	31.0	41.0
46-47	37.23972198668479	39.0	36.0	41.0	31.0	41.0
48-49	37.158150456741126	39.0	36.0	41.0	31.0	41.0
50-51	37.07720058194271	39.0	36.0	41.0	31.0	41.0
52-53	36.98339072865157	39.0	35.0	41.0	31.0	41.0
54-55	36.846854027391345	39.0	35.0	40.0	30.0	41.0
56-57	36.6793582128804	39.0	35.0	40.0	30.0	41.0
58-59	36.52325215844229	38.0	35.0	40.0	30.0	41.0
60-61	36.35690199871025	38.0	34.5	40.0	30.0	41.0
62-63	36.16732070253974	38.0	34.0	40.0	29.0	41.0
64-65	35.956950772072986	38.0	34.0	40.0	29.0	41.0
66-67	35.69447450352561	37.0	34.0	40.0	29.0	41.0
68-69	35.42211161482075	37.0	34.0	39.5	29.0	41.0
70-71	35.1081085301815	36.0	33.5	39.0	28.0	41.0
72-73	34.824688296064124	36.0	33.0	39.0	28.0	40.0
74-75	34.51945674975203	35.0	33.0	38.5	28.0	40.0
76-77	34.19286678724991	35.0	33.0	37.5	28.0	39.5
78-79	34.174521761554914	35.0	33.0	37.0	29.0	39.0
80-81	33.9471609758032	35.0	33.0	37.0	29.0	39.0
82-83	33.666670186119106	35.0	33.0	36.0	28.0	38.5
84-85	33.4440329932998	35.0	33.0	36.0	28.0	37.0
86-87	33.23595293419008	35.0	32.0	35.5	28.0	37.0
88-89	33.190434595482515	35.0	32.5	35.0	28.5	36.5
90-91	33.3021888254532	35.0	33.0	35.0	29.0	36.0
92-93	33.28231715624665	35.0	33.0	35.0	29.0	36.0
94-95	33.25113435348919	35.0	33.0	35.0	29.0	36.0
96-97	33.28487015429724	35.0	33.0	35.0	29.0	36.0
98-99	33.373235675247685	35.0	33.0	35.0	29.0	35.0
100	32.956658926769016	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	20.0
20	149.0
21	1023.0
22	4796.0
23	16352.0
24	41341.0
25	85491.0
26	148233.0
27	227115.0
28	312471.0
29	401340.0
30	493468.0
31	597180.0
32	722877.0
33	883987.0
34	1121619.0
35	1451271.0
36	2039616.0
37	3147027.0
38	3833568.0
39	1735858.0
40	48329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.78353895174867	21.39355095534963	14.152321780141763	24.67058831275994
2	13.894326955633346	22.593500958636152	41.0234701531288	22.488701932601703
3	17.07508482864285	27.991222619492778	31.28115192058193	23.65254063128244
4	11.850844139288903	19.534719817978694	40.01253267894012	28.60190336379228
5	11.964728751079095	39.23121851162972	36.43281189117767	12.371240846113512
6	27.671269067456173	40.35377064515976	19.608397969218522	12.366562318165546
7	24.555843082865742	33.68381283624032	24.77211236923081	16.988231711663133
8	23.665080241375076	37.32508803430088	21.963303244045072	17.046528480278965
9	25.33165275988674	16.303999887253255	21.64300313438269	36.72134421847732
10-11	23.03009714356808	27.347468725773457	30.23495220797062	19.387481922687847
12-13	24.240634482322037	25.137615145215154	30.07314445249613	20.548605919966676
14-15	21.41800925978872	26.28661251357206	27.72556887145473	24.569809355184486
16-17	20.77156954446964	29.35743941970543	28.286585020000416	21.584406015824513
18-19	20.824356046835646	28.565592788551676	30.065306474193182	20.544744690419495
20-21	21.81463470312777	27.711283665586638	29.785173105285086	20.68890852600051
22-23	21.439427961610587	27.694647666896095	29.640626017400184	21.225298354093137
24-25	21.16615628255039	27.834706632342716	29.731454944093162	21.267682141013736
26-27	21.23173053931875	27.926927563306876	29.591953581208468	21.249388316165906
28-29	21.20426588361428	27.962096385785447	29.62515411151247	21.208483619087804
30-31	21.452569004248385	27.814114362968294	29.571052194606068	21.16226443817725
32-33	21.281901304006453	27.976557572130854	29.469353084121018	21.272188039741675
34-35	21.412103860428573	28.016212582688432	29.436077730180234	21.13560582670276
36-37	21.39282642245631	27.95465107952846	29.363362077030793	21.28916042098444
38-39	21.302264966477036	27.969473494190076	29.405011665785853	21.323249873547038
40-41	21.58342945446684	27.846593614605197	29.314067777291612	21.255909153636345
42-43	21.379471115044034	27.972913081839224	29.108765281041094	21.53885052207565
44-45	21.501822338248985	28.104427546900272	28.87674361328436	21.517006501566385
46-47	21.568528388619853	28.116840232182444	28.648438536238608	21.666192842959095
48-49	21.561401741467723	28.287182834090892	28.51197259437692	21.639442830064468
50-51	21.541787595308207	28.39689993754649	28.266994921081068	21.79431754606424
52-53	21.539841222557147	28.226083545701975	28.244295762230127	21.989779469510758
54-55	21.667136209973222	28.0318734422093	28.50711425596057	21.793876091856905
56-57	21.55799271665339	27.822318312890005	28.600067257733624	22.019621712722977
58-59	21.45124997208737	27.715114397484314	28.583644657818247	22.249990972610068
60-61	21.605369983067934	27.840948460490615	28.79874134600331	21.75494021043814
62-63	21.253721942281263	28.036361386311537	28.753632121653062	21.95628454975414
64-65	21.13239999359019	28.263113955447412	28.493314158361926	22.11117189260047
66-67	21.224719292566153	28.709638577797563	28.137142424261043	21.92849970537524
68-69	21.293940915781526	28.708948145141438	27.96764609505167	22.029464844025366
70-71	21.299791659025953	28.271221751614995	27.94836511022516	22.4806214791339
72-73	21.459791065403845	27.95475305881875	27.981922686069673	22.603533189707736
74-75	21.510150227912867	27.979874967739516	28.12848969232386	22.381485112023757
76-77	21.75817732945099	27.91202531601588	27.66194855373712	22.667848800796005
78-79	21.896405730212457	27.990630512608806	27.324026613790263	22.788937143388473
80-81	21.809260047680045	28.241855597972084	27.442632341208583	22.506252013139292
82-83	21.761294626703062	28.053400167018296	27.56338868685454	22.6219165194241
84-85	21.82656753169524	28.38290856880989	27.458385924087796	22.332137975407072
86-87	22.108820587935732	28.31425753563922	27.525686917142984	22.051234959282066
88-89	21.927544700163192	28.38402582367308	27.7887670821339	21.89966239402982
90-91	21.911951938499854	28.093131174402046	27.747931724980624	22.246985162117475
92-93	21.880916119156872	27.762077569485736	27.915492580482866	22.441513730874522
94-95	21.307406101054454	28.156216252421412	28.36953916817041	22.166838478353725
96-97	21.425545126250476	28.166139507865505	28.44455397031467	21.96376139556935
98-99	22.507328537299127	30.059079511459974	28.793408483516163	18.64018346772473
100	21.58171694155846	31.152706291980536	22.485404383102374	24.78017238335863
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2795.0
1	2962.0
2	5286.0
3	7527.0
4	9517.0
5	16557.5
6	22788.0
7	26335.0
8	27827.0
9	25319.0
10	20668.0
11	15642.0
12	15441.5
13	22255.0
14	41674.5
15	70374.0
16	93566.0
17	104799.5
18	105488.5
19	99091.5
20	88897.5
21	78675.5
22	73209.5
23	78191.5
24	94641.5
25	120545.5
26	155161.0
27	194355.0
28	236962.5
29	284381.5
30	328026.5
31	368560.5
32	412794.5
33	453169.0
34	492229.0
35	532077.0
36	571029.5
37	607375.0
38	632562.5
39	649769.0
40	668259.5
41	681587.5
42	688406.0
43	697720.5
44	705245.5
45	710596.5
46	709703.5
47	704276.5
48	696397.0
49	677223.5
50	652221.5
51	622035.5
52	582178.0
53	536697.0
54	491443.0
55	445475.0
56	400276.0
57	355297.0
58	310891.5
59	266982.0
60	219595.0
61	174397.5
62	134787.0
63	102443.5
64	75917.0
65	54710.0
66	39623.0
67	29345.5
68	22022.0
69	15942.5
70	11355.0
71	8232.5
72	5806.5
73	4029.5
74	2758.5
75	1882.5
76	1330.0
77	915.0
78	634.0
79	474.0
80	343.0
81	245.5
82	190.5
83	134.0
84	97.0
85	73.5
86	56.5
87	45.0
88	30.5
89	18.0
90	10.5
91	7.5
92	6.0
93	4.0
94	2.0
95	1.0
96	1.5
97	1.5
98	1.5
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.2243730914987204E-4
24-25	1.473732325073102E-4
26-27	1.5900068474367616E-4
28-29	3.81851136202395E-4
30-31	4.3155935021412005E-4
32-33	4.38212272694796E-4
34-35	8.844693352735798E-4
36-37	4.5245846664803124E-4
38-39	0.0019835940074716743
40-41	8.536679743191947E-5
42-43	1.3918286333205256E-4
44-45	1.0142652832326854E-4
46-47	3.610848288163025E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.2475085694482407E-5
64-65	0.0
66-67	0.0
68-69	5.014707466241436E-4
70-71	0.0
72-73	4.3250087321275926E-4
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	5.234378124833492E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	725.0
20-21	2977.0
22-23	5115.0
24-25	6635.0
26-27	9817.0
28-29	17159.0
30-31	32311.0
32-33	40596.0
34-35	53505.0
36-37	71914.0
38-39	62448.0
40-41	98613.0
42-43	115940.0
44-45	142803.0
46-47	138234.0
48-49	120306.0
50-51	66972.0
52-53	22116.0
54-55	21803.0
56-57	21626.0
58-59	72527.0
60-61	127004.0
62-63	119068.0
64-65	122400.0
66-67	132594.0
68-69	137870.0
70-71	140872.0
72-73	133523.0
74-75	131819.0
76-77	136859.0
78-79	148421.0
80-81	155575.0
82-83	150181.0
84-85	146160.0
86-87	153239.0
88-89	166020.0
90-91	187211.0
92-93	203872.0
94-95	248536.0
96-97	640434.0
98-99	1193920.0
100-101	1.1613418E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.76147429635252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.24236421532966	68.78818116617688
2	8.995908777380823	14.350538934387853
3	2.5311157901873718	6.056565811203662
4	1.0138352762406062	3.2345998530640228
5	0.4651633145346259	1.8551055877929854
6	0.2569267316534906	1.2295712941695451
7	0.1528724737981612	0.85353337226303
8	0.09292564429967554	0.5929509111424455
9	0.06512776253808021	0.4675217721894032
>10	0.17828137489670892	2.10194474481345
>50	0.003926415393047744	0.2160156552270922
>100	0.0014802897861492998	0.20859018576740532
>500	5.7558527334586046E-5	0.03015829295091063
>1k	1.437543425139262E-5	0.01472241885131104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.7759604295882125E-6
2	0.0	2.310384171835285E-5	0.0	5.7759604295882125E-6	5.7759604295882125E-6
3	0.0	2.310384171835285E-5	0.0	5.7759604295882125E-6	5.7759604295882125E-6
4	0.0	5.775960429588212E-5	0.0	3.465576257752927E-5	5.7759604295882125E-6
5	1.7327881288764637E-5	5.775960429588212E-5	0.0	5.1983643866293906E-5	5.7759604295882125E-6
6	1.7327881288764637E-5	5.775960429588212E-5	0.0	5.775960429588212E-5	1.7327881288764637E-5
7	1.7327881288764637E-5	5.775960429588212E-5	0.0	1.2129516902135245E-4	1.7327881288764637E-5
8	2.887980214794106E-5	6.931152515505855E-5	0.0	1.5595093159888172E-4	1.7327881288764637E-5
9	2.887980214794106E-5	1.5017497116929352E-4	0.0	1.90606694176411E-4	1.7327881288764637E-5
10-11	4.62076834367057E-5	1.5017497116929352E-4	0.0	2.1371053589476385E-4	1.7327881288764637E-5
12-13	5.1983643866293906E-5	1.5306295138408762E-4	0.0	2.1948649632435205E-4	1.7327881288764637E-5
14-15	6.0647584510676226E-5	2.1371053589476383E-4	0.0	2.8013408083502826E-4	1.7327881288764637E-5
16-17	8.952738665861729E-5	2.1948649632435205E-4	0.0	3.234537840569399E-4	2.310384171835285E-5
18-19	1.1551920859176424E-4	2.512542786870872E-4	0.0	3.6966146749364554E-4	2.887980214794106E-5
20-21	1.5306295138408762E-4	2.887980214794106E-4	0.0	4.274210717895277E-4	2.887980214794106E-5
22-23	1.7905477331723457E-4	3.0612590276817524E-4	0.0	4.4186097286349823E-4	4.331970322191159E-5
24-25	2.2815043696873438E-4	3.3789368513091036E-4	0.0	5.140604782333509E-4	6.0647584510676226E-5
26-27	2.7435812040544006E-4	3.6388550706405734E-4	0.0	6.411316076842915E-4	6.353556472547033E-5
28-29	2.859100412646165E-4	3.6677348727885147E-4	0.0	0.0012620473538650244	6.353556472547033E-5
30-31	2.887980214794106E-4	4.0431723007117485E-4	0.0	0.0035002320203304568	6.353556472547033E-5
32-33	3.1190186319776344E-4	4.07205210285969E-4	0.0	0.008193199869370879	6.353556472547033E-5
34-35	3.4655762577529275E-4	4.7074077501143923E-4	0.0	0.01392584059573718	6.353556472547033E-5
36-37	3.6677348727885147E-4	5.313883595221155E-4	0.0	0.026491442510306336	6.353556472547033E-5
38-39	3.696614674936456E-4	5.544922012404684E-4	0.0	0.13654948051589494	6.353556472547033E-5
40-41	3.841013685676161E-4	6.411316076842915E-4	0.0	0.25610608544794133	6.931152515505855E-5
42-43	4.0431723007117485E-4	6.786753504766149E-4	0.0	0.287824772147025	6.931152515505855E-5
44-45	4.1009319050076305E-4	7.768666777796145E-4	0.0	0.33041381637459366	7.219950536985266E-5
46-47	4.303090520043218E-4	8.317383018607025E-4	0.0	0.3739270142708965	7.508748558464676E-5
48-49	4.331970322191159E-4	8.375142622902908E-4	0.0	0.416111741268394	8.663940644382319E-5
50-51	4.331970322191159E-4	9.674733719560256E-4	0.0	0.4592841574993511	8.663940644382319E-5
52-53	4.389729926487041E-4	9.761373126004079E-4	0.0	0.8417711451268972	8.952738665861729E-5
54-55	4.4186097286349823E-4	9.848012532447901E-4	0.0	1.3338656458465241	9.24153668734114E-5
56-57	4.4474895307829236E-4	0.0010165690356075253	0.0	1.871535362335817	9.24153668734114E-5
58-59	4.534128937226747E-4	0.0010223449960371136	0.0	2.444929394082113	9.24153668734114E-5
60-61	4.736287552262334E-4	0.0010512247981850544	0.0	2.60150990536782	9.24153668734114E-5
62-63	4.765167354410275E-4	0.0010685526794738192	0.0	2.6081060521784094	9.819132730299961E-5
64-65	4.851806760854098E-4	0.00119562380892476	0.0	2.6138675727069236	9.819132730299961E-5
66-67	5.025085573741744E-4	0.0012042877495691423	0.0	2.6188175707950805	9.819132730299961E-5
68-69	5.082845178037627E-4	0.0012649353340798185	0.0	2.623724249180016	9.819132730299961E-5
70-71	5.082845178037627E-4	0.0012995910966573476	0.0	2.627813629164164	9.819132730299961E-5
72-73	5.082845178037627E-4	0.0013053670570869359	0.0	2.630649625735092	1.0396728773258781E-4
74-75	5.082845178037627E-4	0.0013342468592348771	0.0	2.63291957818392	1.0396728773258781E-4
76-77	5.082845178037627E-4	0.0014324381865378766	0.0	2.6353888012675695	1.0396728773258781E-4
78-79	5.082845178037627E-4	0.0014670939491154059	0.0	2.6369743024054912	1.0396728773258781E-4
80-81	5.256123990925273E-4	0.0014670939491154059	0.0	2.637549010468235	1.1263122837697014E-4
82-83	5.342763397369096E-4	0.0014728699095449941	0.0	2.637982207500454	1.2129516902135245E-4
84-85	5.42940280381292E-4	0.0014873098106189646	0.0	2.6381785901550603	1.2129516902135245E-4
86-87	5.42940280381292E-4	0.0015075256721225234	0.0	2.6382710055219336	1.2418314923614657E-4
88	5.42940280381292E-4	0.0016114929598551112	0.0	2.638279669462578	1.2707112945094067E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23285	0.0	50.40832	1
ATCTCGT	10595	0.0	41.685528	94
GTATCAA	41955	0.0	37.015976	1
TATCTCG	13540	0.0	35.297146	94
ATCAACG	51945	0.0	29.362484	3
TCAACGC	52070	0.0	29.266998	4
CAACGCA	52660	0.0	28.972052	5
TATCAAC	54430	0.0	28.253134	2
AACGCAG	54475	0.0	28.070486	6
TATGCCG	5830	0.0	26.712196	86-87
GCCGTCT	6355	0.0	26.400154	90-91
ATGCCGT	6335	0.0	26.31796	88-89
TCTCGTA	6180	0.0	25.15623	82-83
TGCCGTC	6245	0.0	24.84388	88-89
ACGCAGA	62745	0.0	24.287714	7
CTCGTAT	6190	0.0	24.13318	82-83
CCGTCTT	6580	0.0	24.128426	90-91
TCGTATG	6845	0.0	24.084852	84-85
CGCAGAG	63350	0.0	24.042063	8
CGTATGC	6815	0.0	22.737852	84-85
>>END_MODULE