FastQCFastQC Report
Fri 27 May 2016
SRR2139292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20567476
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA277700.051.9312251
GTATCAA519350.038.7562031
ATCAACG655500.030.1565363
TCAACGC659250.029.9915054
CAACGCA667550.029.573615
TATCAAC690400.028.781272
AACGCAG692000.028.5535096
ACGCAGA794150.024.8591127
CGCAGAG800050.024.6704258
GTACATG763650.021.497431
TACATGG758500.021.2530652
GCAGAGT942300.020.8368849
ACATGGG767400.020.6095963
AGAGTAC869250.019.30047610-11
GAGTACT523700.018.82796512-13
CAGAGTA926300.018.28316510-11
CATGGGG464050.017.6420694
GTGGTAT121350.017.3611181
AGTACTT572350.017.1563612-13
GTACTTT597700.016.44423114-15
Produced by FastQC (version 0.11.5)