##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139292_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20567476
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60600722227657	31.0	31.0	34.0	30.0	34.0
2	31.571545336919318	31.0	31.0	34.0	30.0	34.0
3	31.73833405712981	31.0	31.0	34.0	30.0	34.0
4	35.49926490737123	37.0	35.0	37.0	33.0	37.0
5	35.42025778953139	37.0	35.0	37.0	33.0	37.0
6	35.488546139545754	37.0	35.0	37.0	33.0	37.0
7	35.38666144547829	37.0	35.0	37.0	33.0	37.0
8	35.38625702055031	37.0	35.0	37.0	33.0	37.0
9	36.91396912290064	39.0	37.0	39.0	33.0	39.0
10-11	36.84231840114947	39.0	37.0	39.0	32.5	39.0
12-13	36.804462054556424	39.0	37.0	39.0	32.0	39.0
14-15	37.956923372610234	40.0	37.0	41.0	33.0	41.0
16-17	37.97251801825367	40.0	37.0	41.0	33.0	41.0
18-19	38.03928389171331	40.0	37.0	41.0	33.0	41.0
20-21	38.065450097191885	40.0	37.0	41.0	33.0	41.0
22-23	38.02923952691401	40.0	37.0	41.0	33.0	41.0
24-25	37.980366641706766	40.0	37.0	41.0	33.0	41.0
26-27	37.8462518284156	40.0	37.0	41.0	32.0	41.0
28-29	37.766679465728984	40.0	37.0	41.0	32.0	41.0
30-31	37.675146745578026	40.0	37.0	41.0	32.0	41.0
32-33	37.57057842287516	40.0	36.0	41.0	32.0	41.0
34-35	37.48744492222173	39.5	36.0	41.0	31.5	41.0
36-37	37.427198991935406	39.0	36.0	41.0	31.0	41.0
38-39	37.368519185386155	39.0	36.0	41.0	31.0	41.0
40-41	37.316773615622715	39.0	36.0	41.0	31.0	41.0
42-43	37.25414617347249	39.0	36.0	41.0	31.0	41.0
44-45	37.19595575270779	39.0	36.0	41.0	31.0	41.0
46-47	37.15161049740085	39.0	36.0	41.0	31.0	41.0
48-49	37.07297157282717	39.0	36.0	41.0	31.0	41.0
50-51	36.993261632378456	39.0	35.0	41.0	30.5	41.0
52-53	36.90338185690263	39.0	35.0	40.0	30.0	41.0
54-55	36.76742502353199	39.0	35.0	40.0	30.0	41.0
56-57	36.604630147548754	38.5	35.0	40.0	30.0	41.0
58-59	36.44782844948969	38.0	35.0	40.0	30.0	41.0
60-61	36.28445926269613	38.0	34.0	40.0	29.0	41.0
62-63	36.091131541445684	38.0	34.0	40.0	29.0	41.0
64-65	35.870001533556604	37.5	34.0	40.0	29.0	41.0
66-67	35.607181648148924	37.0	34.0	40.0	28.5	41.0
68-69	35.343747601100574	37.0	34.0	39.0	28.0	41.0
70-71	35.03363932604884	36.0	33.0	39.0	28.0	40.5
72-73	34.74699889662094	36.0	33.0	39.0	28.0	40.0
74-75	34.45389436450128	35.0	33.0	38.0	28.0	40.0
76-77	34.13554927176489	35.0	33.0	37.5	28.0	39.5
78-79	34.124628911151504	35.0	33.0	37.0	28.0	39.0
80-81	33.91589377788904	35.0	33.0	37.0	29.0	39.0
82-83	33.64331275199892	35.0	33.0	36.0	28.0	38.5
84-85	33.410813085443046	35.0	32.5	36.0	28.0	37.0
86-87	33.21193927937	35.0	32.0	35.5	28.0	37.0
88-89	33.17451591881475	35.0	32.5	35.0	28.5	36.5
90-91	33.27531313281339	35.0	33.0	35.0	29.0	36.0
92-93	33.25347237655117	35.0	33.0	35.0	29.0	36.0
94-95	33.237601831217574	35.0	33.0	35.0	29.0	36.0
96-97	33.28381594317693	35.0	33.0	35.0	29.0	36.0
98-99	33.36678995697963	35.0	33.0	35.0	29.0	35.0
100	32.96391357672456	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	22.0
20	197.0
21	1307.0
22	6091.0
23	20409.0
24	51941.0
25	106238.0
26	182546.0
27	276945.0
28	379240.0
29	486525.0
30	598515.0
31	725243.0
32	875445.0
33	1072570.0
34	1351787.0
35	1744002.0
36	2424248.0
37	3692400.0
38	4435030.0
39	2079391.0
40	57380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.8519925342322	21.374810404543563	14.161883548569595	24.61131351265464
2	13.961100525898267	22.688379458908813	40.93529026118713	22.415229754005793
3	17.18263339653344	28.02006916162197	31.220374342481307	23.576923099363288
4	11.865943103567982	19.558656589656405	39.90680723293417	28.668593073841436
5	11.963568111128463	39.35168807295557	36.2769452119453	12.407798603970658
6	27.581473779281424	40.29990359536581	19.648473152466543	12.47014947288622
7	24.62251566502375	33.76217869416743	24.620609743266506	16.994695897542314
8	23.726815093889012	37.24295095810492	21.96744510604996	17.062788841956106
9	25.48076390121958	16.2933701733747	21.577280556933676	36.64858536847205
10-11	23.065965896836346	27.380505998888733	30.153792327264657	19.399735777010267
12-13	24.31462907748136	25.197710210042302	30.00283311379578	20.484827598680557
14-15	21.459983714093067	26.306681967199086	27.72412375733415	24.509210561373695
16-17	20.723033784019	29.408221990875305	28.293651588556614	21.575092636549083
18-19	20.7932319940473	28.559554414941335	30.090002292940564	20.557211298070797
20-21	21.895273920094034	27.685869751969765	29.802033315907373	20.616823012028824
22-23	21.500418536691456	27.651042820690243	29.683287459325687	21.165251183292614
24-25	21.156525784197772	27.813031805729953	29.79293955968781	21.23750285038446
26-27	21.195272570122068	27.91892685034948	29.65649506953076	21.229305509997694
28-29	21.169428198990833	27.952348227726198	29.7091362561125	21.169087317170472
30-31	21.416603762067968	27.836027200528648	29.599240298687267	21.148128738716114
32-33	21.25822497665358	27.946673085644385	29.545888994751113	21.24921294295092
34-35	21.355083045068348	27.98551684038707	29.533261790097352	21.126138324447226
36-37	21.356493559798036	27.896612201212218	29.47115098127767	21.275743257712076
38-39	21.27117600718263	28.00837282355562	29.494497192651675	21.225953976610075
40-41	21.49471463016476	27.901192250960378	29.337326876569296	21.266766242305568
42-43	21.391119161614284	28.010497565968983	29.1487346600092	21.44964861240753
44-45	21.50095938323474	28.170331222453846	28.888300815187833	21.44040857912358
46-47	21.600057681568313	28.159648962396467	28.587686487077818	21.652606868957402
48-49	21.56804341587832	28.271557686311038	28.5111998843463	21.649199013464337
50-51	21.56407508948045	28.387733365979066	28.3489837404922	21.699207804048285
52-53	21.653038251306317	28.29966333153417	28.29982152455699	21.747476892602524
54-55	21.67335923681378	28.242539931720707	28.36815116799667	21.715949663468844
56-57	21.65651307736996	28.246074541255616	28.37785973100245	21.719552650371977
58-59	21.6387196230806	28.28548438676666	28.337881621767032	21.737914368385706
60-61	21.674882244568213	28.290102359054032	28.334863951789384	21.70015144458837
62-63	21.627405392112703	28.327580047371615	28.305949307766625	21.739065252749054
64-65	21.59217198756047	28.325360768809887	28.28218736235042	21.800279881279224
66-67	21.66161458482166	28.39850793208646	28.111960825943672	21.82791665714821
68-69	21.672362536677024	28.36038044467185	28.139771080237004	21.82748593841412
70-71	21.67166709043139	28.16021919056769	28.246344855565695	21.92176886343522
72-73	21.698809781442304	28.15003029649278	28.197368215865847	21.953791706199073
74-75	21.616484706871205	28.313014160951074	28.150424373521815	21.920076758655906
76-77	21.741548299266896	28.245648948769574	28.08775024469131	21.925052507272213
78-79	21.721129634408364	28.273796963845786	28.05976859292201	21.945304808823845
80-81	21.711283746803616	28.288990052071306	28.028100502828725	21.97162569829636
82-83	21.743801522216334	28.279658571788346	28.148694271676632	21.827845634318688
84-85	21.832630392028946	28.29085806348238	28.067081265410394	21.809430279078285
86-87	21.687949550752585	28.331396247627783	28.13463352574687	21.84602067587276
88-89	21.752071950495854	28.30775917334793	28.092285627389902	21.847883248766315
90-91	21.774758745442117	28.287825771061968	28.174574079007414	21.7628414044885
92-93	21.77067309684607	28.30000102116239	28.15999559807186	21.76933028391968
94-95	21.6043385735368	28.356872048408434	28.24109393913113	21.797695438923636
96-97	21.63285788080049	28.483283897181277	28.31522346001015	21.56863476200808
98-99	22.633913947351356	30.429949719620065	28.57367507124824	18.362461261780343
100	21.66258462062909	31.418336229671596	22.365197994671725	24.553881155027586
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3403.0
1	3515.5
2	6208.0
3	8894.5
4	11058.5
5	18808.0
6	25798.5
7	30204.5
8	32348.5
9	29931.5
10	24732.0
11	18882.5
12	19038.5
13	27360.5
14	50673.0
15	85325.0
16	113574.0
17	127736.5
18	129198.0
19	122084.5
20	109712.5
21	97029.5
22	91116.0
23	98631.0
24	119851.5
25	153837.5
26	199162.5
27	248547.5
28	299633.0
29	355197.0
30	408033.0
31	458797.5
32	513670.5
33	564755.0
34	613086.5
35	658192.0
36	700943.0
37	741610.5
38	769162.0
39	787222.0
40	807649.5
41	821758.0
42	826283.0
43	831899.0
44	836949.5
45	841767.0
46	835995.5
47	823488.0
48	811203.0
49	787824.0
50	761499.5
51	729106.0
52	684467.0
53	635992.5
54	587049.0
55	536148.0
56	484750.5
57	431653.0
58	377341.5
59	326118.5
60	272531.5
61	217524.0
62	168206.0
63	127760.5
64	95137.0
65	68667.5
66	49228.5
67	36085.5
68	26534.5
69	19163.5
70	13503.5
71	9407.5
72	6494.0
73	4408.0
74	3001.5
75	2063.5
76	1487.0
77	1045.0
78	734.0
79	545.0
80	408.5
81	300.5
82	230.0
83	170.0
84	125.5
85	93.0
86	68.5
87	46.0
88	30.5
89	21.5
90	14.0
91	11.0
92	6.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.8693462632236135E-4
24-25	1.6296571527282092E-4
26-27	1.5086794572511053E-4
28-29	4.3340500748354096E-4
30-31	3.997796141746445E-4
32-33	3.9564869473175446E-4
34-35	8.960574134832698E-4
36-37	4.2493514519456686E-4
38-39	0.0019022053764515018
40-41	6.945632884952214E-5
42-43	1.1480264564194307E-4
44-45	9.052437602679159E-5
46-47	1.5222086435573408E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.880699897630818E-5
64-65	0.0
66-67	0.0
68-69	4.652964557900913E-4
70-71	0.0
72-73	4.066815022208877E-4
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.0070409261878505E-6
88-89	0.0
90-91	0.0
92-93	5.152627702724984E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	851.0
20-21	3216.0
22-23	5390.0
24-25	7773.0
26-27	11069.0
28-29	19242.0
30-31	36680.0
32-33	46216.0
34-35	62209.0
36-37	84808.0
38-39	104432.0
40-41	118448.0
42-43	141017.0
44-45	173615.0
46-47	170072.0
48-49	148329.0
50-51	126072.0
52-53	112571.0
54-55	120554.0
56-57	136922.0
58-59	143601.0
60-61	148888.0
62-63	141411.0
64-65	145773.0
66-67	157231.0
68-69	165022.0
70-71	169058.0
72-73	158384.0
74-75	156741.0
76-77	163051.0
78-79	176637.0
80-81	185990.0
82-83	178857.0
84-85	174767.0
86-87	183142.0
88-89	197691.0
90-91	222715.0
92-93	239070.0
94-95	291699.0
96-97	724485.0
98-99	1319727.0
100-101	1.349405E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.67833918637005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.42932958402012	71.41082436600534
2	8.507536143534832	13.897628455438815
3	2.2168963411555285	5.43217233881772
4	0.8528008316674665	2.786214223494152
5	0.3983300439095183	1.6267468217281658
6	0.21080303124098812	1.0330824892329835
7	0.11573358468087427	0.6617048889373253
8	0.06888504798628105	0.4501133051434273
9	0.0493048565131707	0.36244249144362567
>10	0.14440801123229624	1.8082445527535735
>50	0.004222519245108003	0.2389204619355276
>100	0.0016554765139554827	0.225918415224522
>500	7.096680638763996E-5	0.035984851818681766
>1k	2.3561493471080344E-5	0.03000233802615503
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.862045299092606E-6
2	0.0	0.0	0.0	0.0	9.724090598185213E-6
3	0.0	0.0	0.0	0.0	9.724090598185213E-6
4	0.0	2.9172271794555637E-5	0.0	9.724090598185213E-6	1.4586135897277818E-5
5	4.862045299092606E-6	2.9172271794555637E-5	0.0	1.9448181196370426E-5	1.4586135897277818E-5
6	4.862045299092606E-6	2.9172271794555637E-5	0.0	1.9448181196370426E-5	1.9448181196370426E-5
7	4.862045299092606E-6	2.9172271794555637E-5	0.0	4.3758407691833455E-5	1.9448181196370426E-5
8	4.862045299092606E-6	3.403431709364824E-5	0.0	4.862045299092606E-5	1.9448181196370426E-5
9	4.862045299092606E-6	7.293067948638909E-5	0.0	1.0210295128094473E-4	2.431022649546303E-5
10-11	4.862045299092606E-6	7.293067948638909E-5	0.0	1.2398215512686146E-4	2.431022649546303E-5
12-13	1.9448181196370426E-5	7.293067948638909E-5	0.0	1.3127522307550038E-4	2.431022649546303E-5
14-15	2.4310226495463033E-5	1.0210295128094473E-4	0.0	1.7260260811778752E-4	4.618943034137976E-5
16-17	3.6465339743194546E-5	1.0696499658003734E-4	0.0	2.0420590256188946E-4	6.320658888820388E-5
18-19	4.132738504228715E-5	1.1668908717822255E-4	0.0	2.2365408375825987E-4	7.049965683684279E-5
20-21	4.3758407691833455E-5	1.2155113247731516E-4	0.0	2.6741249145009337E-4	7.293067948638909E-5
22-23	5.591352093956497E-5	1.482923816223245E-4	0.0	2.96584763244649E-4	8.994783803321322E-5
24-25	7.77927247854817E-5	1.60447494870056E-4	0.0	3.4763623888512134E-4	1.1182704187912994E-4
26-27	8.0223747435028E-5	1.653095401691486E-4	0.0	4.5460123546515866E-4	1.1182704187912994E-4
28-29	8.26547700845743E-5	1.677405628186949E-4	0.0	9.310816747762341E-4	1.1182704187912994E-4
30-31	8.26547700845743E-5	1.8961976666461165E-4	0.0	0.0026838490050991185	1.1425806452867624E-4
32-33	9.724090598185212E-5	1.8961976666461165E-4	0.0	0.006393589568306777	1.1668908717822255E-4
34-35	1.0210295128094473E-4	2.309471517068988E-4	0.0	0.010832636926378328	1.1668908717822255E-4
36-37	1.1182704187912994E-4	2.722745367491859E-4	0.0	0.019579456419445924	1.1668908717822255E-4
38-39	1.1182704187912994E-4	2.7713658204827854E-4	0.0	0.046126223752491556	1.2884420042595407E-4
40-41	1.1912010982776885E-4	3.2575703503920464E-4	0.0	0.07679357447651816	1.4099931367368557E-4
42-43	1.2155113247731516E-4	3.330501029878435E-4	0.0	0.09479529719640854	1.4343033632323188E-4
44-45	1.2641317777640777E-4	3.646533974319455E-4	0.0	0.11666720797437663	1.482923816223245E-4
46-47	1.3370624572504668E-4	3.889636239274085E-4	0.0	0.13765422650790987	1.555854495709634E-4
48-49	1.3613726837459296E-4	3.889636239274085E-4	0.0	0.15736009610513219	1.653095401691486E-4
50-51	1.3613726837459296E-4	4.3029100896969566E-4	0.0	0.17765670420619428	1.653095401691486E-4
52-53	1.4343033632323188E-4	4.375840769183346E-4	0.0	0.23464473715685874	1.701715854682412E-4
54-55	1.507234042718708E-4	4.4487714486697344E-4	0.0	0.30258452714372924	1.7746465341688013E-4
56-57	1.507234042718708E-4	4.5703225811470497E-4	0.0	0.3656112203558667	1.8475772136551904E-4
58-59	1.555854495709634E-4	4.643253260633439E-4	0.0	0.3950946630495643	1.9205078931415796E-4
60-61	1.555854495709634E-4	4.740494166615291E-4	0.0	0.4144966548155933	1.9448181196370424E-4
62-63	1.555854495709634E-4	4.910665752083532E-4	0.0	0.4297537529641459	1.9934385726279685E-4
64-65	1.555854495709634E-4	5.494111187974645E-4	0.0	0.4370322347768876	1.9934385726279685E-4
66-67	1.555854495709634E-4	5.61566232045196E-4	0.0	0.44361544411185894	2.0663692521143576E-4
68-69	1.555854495709634E-4	5.810144132415664E-4	0.0	0.4502813082169149	2.1636101580962098E-4
70-71	1.580164722205097E-4	5.907385038397517E-4	0.0	0.4551239053348111	2.187920384591673E-4
72-73	1.653095401691486E-4	5.956005491388443E-4	0.0	0.4585832505651155	2.2608510640780618E-4
74-75	1.701715854682412E-4	6.101866850361221E-4	0.0	0.4611941688907283	2.2851612905735248E-4
76-77	1.701715854682412E-4	6.685312286252333E-4	0.0	0.4637491736954015	2.2851612905735248E-4
78-79	1.701715854682412E-4	6.855483871720575E-4	0.0	0.46549707898042525	2.2851612905735248E-4
80-81	1.7260260811778752E-4	6.855483871720575E-4	0.0	0.46606107623511994	2.4310226495463032E-4
82-83	1.7503363076733382E-4	6.855483871720575E-4	0.0	0.466430591677851	2.6011942350145445E-4
84-85	1.7503363076733382E-4	6.855483871720575E-4	0.0	0.4665570048556274	2.6255044615100076E-4
86-87	1.7503363076733382E-4	6.97703500419789E-4	0.0	0.4666226424671651	2.6498146880054706E-4
88	1.7503363076733382E-4	7.633411119575392E-4	0.0	0.4666250734898147	2.674124914500933E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27770	0.0	51.931225	1
GTATCAA	51935	0.0	38.756203	1
ATCAACG	65550	0.0	30.156536	3
TCAACGC	65925	0.0	29.991505	4
CAACGCA	66755	0.0	29.57361	5
TATCAAC	69040	0.0	28.78127	2
AACGCAG	69200	0.0	28.553509	6
ACGCAGA	79415	0.0	24.859112	7
CGCAGAG	80005	0.0	24.670425	8
GTACATG	76365	0.0	21.49743	1
TACATGG	75850	0.0	21.253065	2
GCAGAGT	94230	0.0	20.836884	9
ACATGGG	76740	0.0	20.609596	3
AGAGTAC	86925	0.0	19.300476	10-11
GAGTACT	52370	0.0	18.827965	12-13
CAGAGTA	92630	0.0	18.283165	10-11
CATGGGG	46405	0.0	17.642069	4
GTGGTAT	12135	0.0	17.361118	1
AGTACTT	57235	0.0	17.15636	12-13
GTACTTT	59770	0.0	16.444231	14-15
>>END_MODULE