FastQCFastQC Report
Fri 27 May 2016
SRR2139291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41327236
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA523250.048.7534941
GTATCAA930000.036.4828381
TCAACGC1152050.028.8794024
ATCAACG1156700.028.7558653
CAACGCA1175650.028.2923565
TATCAAC1218050.027.4771182
AACGCAG1216100.027.422086
ACGCAGA1417000.023.4886597
CGCAGAG1434000.023.1801878
GTACATG1210600.020.7453251
TACATGG1194350.020.5843242
ACATGGG1206800.019.8512553
GCAGAGT1700000.019.4164529
AGAGTAC1522650.018.63220410-11
GAGTACT1006650.018.38740712-13
CATGGGG826100.017.6414554
CAGAGTA1646900.017.57670210-11
AGTACTT1105800.016.6511512-13
GTGGTAT233200.016.4817371
GTACTTT1151150.016.08236514-15
Produced by FastQC (version 0.11.5)