FastQCFastQC Report
Fri 27 May 2016
SRR2139290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33990618
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA447250.050.7419741
GTATCAA812950.037.1103631
TCAACGC1022550.028.8772624
ATCAACG1029900.028.6920413
CAACGCA1048350.028.182995
TATCAAC1077850.027.6150152
AACGCAG1084550.027.2779676
ACGCAGA1250400.023.6426967
CGCAGAG1269450.023.2642028
GTACATG1035800.021.2999781
TACATGG1046450.020.6560342
ACATGGG1048200.020.0556763
GCAGAGT1476800.019.8348069
AGAGTAC1335050.018.95954110-11
GAGTACT884750.018.4412112-13
CAGAGTA1439400.017.82394810-11
CATGGGG701200.017.5739574
AGTACTT955000.017.11392612-13
GTGGTAT209900.016.9551531
GTACTTT993700.016.4098914-15
Produced by FastQC (version 0.11.5)