##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139290_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33990618
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623387753644256	31.0	31.0	34.0	30.0	34.0
2	31.59326299980777	31.0	31.0	34.0	30.0	34.0
3	31.755320629945594	31.0	31.0	34.0	30.0	34.0
4	35.51347286477698	37.0	35.0	37.0	33.0	37.0
5	35.427211061593525	37.0	35.0	37.0	33.0	37.0
6	35.49424861883947	37.0	35.0	37.0	33.0	37.0
7	35.38827469980099	37.0	35.0	37.0	33.0	37.0
8	35.38929807042638	37.0	35.0	37.0	33.0	37.0
9	36.91565469624589	39.0	37.0	39.0	33.0	39.0
10-11	36.84216385827406	39.0	37.0	39.0	32.5	39.0
12-13	36.798991401097794	39.0	37.0	39.0	32.0	39.0
14-15	37.95571675101641	40.0	37.0	41.0	33.0	41.0
16-17	37.96594854497791	40.0	37.0	41.0	33.0	41.0
18-19	38.025867534976854	40.0	37.0	41.0	33.0	41.0
20-21	38.04715738075617	40.0	37.0	41.0	33.0	41.0
22-23	38.00971236796538	40.0	37.0	41.0	33.0	41.0
24-25	37.959620369301824	40.0	37.0	41.0	33.0	41.0
26-27	37.819924969155466	40.0	37.0	41.0	32.0	41.0
28-29	37.73761433194795	40.0	37.0	41.0	32.0	41.0
30-31	37.64438193805279	40.0	37.0	41.0	32.0	41.0
32-33	37.53663261564688	40.0	36.0	41.0	32.0	41.0
34-35	37.45233142576778	39.0	36.0	41.0	31.0	41.0
36-37	37.38918039356	39.0	36.0	41.0	31.0	41.0
38-39	37.32658886510354	39.0	36.0	41.0	31.0	41.0
40-41	37.27183030803498	39.0	36.0	41.0	31.0	41.0
42-43	37.20619967304368	39.0	36.0	41.0	31.0	41.0
44-45	37.1436976130004	39.0	36.0	41.0	31.0	41.0
46-47	37.09232434388865	39.0	36.0	41.0	31.0	41.0
48-49	37.00769457596156	39.0	35.0	41.0	30.5	41.0
50-51	36.918358237576555	39.0	35.0	40.5	30.0	41.0
52-53	36.82084210704712	39.0	35.0	40.0	30.0	41.0
54-55	36.678072206998344	39.0	35.0	40.0	30.0	41.0
56-57	36.509274685914974	38.0	35.0	40.0	30.0	41.0
58-59	36.34284984642476	38.0	34.5	40.0	29.5	41.0
60-61	36.16651253491872	38.0	34.0	40.0	29.0	41.0
62-63	35.96142450199441	38.0	34.0	40.0	29.0	41.0
64-65	35.724355187078984	37.0	34.0	40.0	29.0	41.0
66-67	35.450789066922646	37.0	34.0	40.0	28.0	41.0
68-69	35.181199990009944	36.0	34.0	39.0	28.0	41.0
70-71	34.86621609414434	36.0	33.0	39.0	28.0	40.5
72-73	34.57601341469986	35.5	33.0	38.5	28.0	40.0
74-75	34.28671126328484	35.0	33.0	38.0	28.0	40.0
76-77	33.97884124435995	35.0	32.5	37.0	28.0	39.0
78-79	33.97235098811325	35.0	33.0	37.0	28.0	39.0
80-81	33.77529166892377	35.0	33.0	36.5	28.0	39.0
82-83	33.52122395391591	35.0	32.5	36.0	28.0	38.0
84-85	33.303633990235866	35.0	32.0	36.0	28.0	37.0
86-87	33.117329696414004	35.0	32.0	35.0	28.0	37.0
88-89	33.09037181414199	35.0	32.0	35.0	28.5	36.5
90-91	33.20770989871162	35.0	33.0	35.0	29.0	36.0
92-93	33.19745912645173	35.0	33.0	35.0	29.0	36.0
94-95	33.18900587686791	35.0	33.0	35.0	29.0	36.0
96-97	33.2390595620853	35.0	33.0	35.0	29.0	35.5
98-99	33.32411679026234	35.0	33.0	35.0	29.0	35.0
100	32.922922292380925	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	6.0
19	43.0
20	358.0
21	2305.0
22	10327.0
23	34879.0
24	88707.0
25	180964.0
26	312412.0
27	470921.0
28	647516.0
29	828535.0
30	1016110.0
31	1226357.0
32	1477571.0
33	1804320.0
34	2275626.0
35	2940528.0
36	4108687.0
37	6214000.0
38	7110670.0
39	3152778.0
40	86996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.02730106289918	21.080667024059405	14.124597558067348	24.767434354974068
2	14.200718562986998	22.382308553495555	40.582933796614114	22.83403908690333
3	17.3612083193074	27.496711004195333	31.066696110085434	24.075384566411824
4	12.218904051700385	19.248605600521884	39.27527884312077	29.25721150465696
5	12.287637724033143	38.866524874599214	36.13585960690682	12.709977794460814
6	28.33145310861956	39.84749850679384	19.146112612603865	12.674935771982728
7	24.972355607067808	33.412222749230395	24.34456766864315	17.270853975058646
8	23.879092165961797	37.208046643929805	21.604796947204665	17.308064242903733
9	25.336358991766495	16.36568655503704	21.39091145680258	36.90704299639388
10-11	23.41651893472487	27.082342251029385	29.824145886373703	19.67699292787204
12-13	24.538416747821415	24.826905177187424	29.757252427714025	20.87742564727714
14-15	21.750609241644266	25.995139305793145	27.25493840682744	24.99931304573515
16-17	21.15761325669336	29.097092321181094	27.858841813349787	21.88645260877575
18-19	21.224084540033957	28.250941186182608	29.62419659448381	20.900777679299622
20-21	22.129773141529665	27.417439362970814	29.41766185313713	21.035125642362388
22-23	21.803406547953415	27.33255848674996	29.335224625636936	21.52881033965969
24-25	21.531693773516427	27.514720609951915	29.392288942675744	21.561296673855914
26-27	21.624590286272017	27.566877231157733	29.254032848249086	21.554499634321168
28-29	21.59023836278432	27.519443775081882	29.325710429108177	21.56460743302562
30-31	21.836105882238744	27.452473909409463	29.15285245646066	21.558567751891133
32-33	21.652673589283506	27.654431899217858	29.078458096123356	21.61443641537528
34-35	21.78483692946143	27.624415716167235	29.079465920061892	21.51128143430945
36-37	21.801795368505374	27.54969213384268	29.004925641493802	21.643586856158148
38-39	21.686645881868056	27.66708210580788	29.05312009547903	21.593151916845038
40-41	21.893838406394053	27.562064557706773	28.888506068819964	21.655590967079213
42-43	21.79170296294702	27.699760944862504	28.674547065844568	21.833989026345908
44-45	21.917648666813676	27.76540798136623	28.482581790413043	21.83436156140705
46-47	22.04148618706089	27.82108173072385	28.139012219273322	21.998419862941933
48-49	21.971245301012893	27.95661576074739	28.061748368998728	22.010390569240986
50-51	21.98192121204641	28.07060531630492	27.94873067980626	21.99874279184241
52-53	22.139346474796813	27.92614583942474	27.880970071806882	22.05353761397156
54-55	22.073995726314593	27.848145266849134	27.965519043707047	22.11233996312923
56-57	22.070132361595654	27.950398617279298	27.959029019082358	22.020440002042687
58-59	22.081137223076713	27.992188918518814	27.881821901361388	22.044851957043086
60-61	22.12412989130399	27.963309436406565	27.91129119426119	22.001269478028256
62-63	22.084894014325993	28.036375557017113	27.898464807202615	21.980265621454283
64-65	22.10749387199391	27.972125106763524	27.84603553112338	22.074345490119182
66-67	22.122978992540627	27.998725259947776	27.75688724949643	22.121408498015164
68-69	22.097307008084446	27.959005222069656	27.81726031356969	22.126427456276204
70-71	22.10710140166264	27.789977788299915	27.878425254558703	22.224495555478747
72-73	22.1317023013712	27.858799846186077	27.78192165750194	22.227576194940784
74-75	21.959678910804104	27.990517419525112	27.829082598688952	22.220721070981828
76-77	22.164522201834156	27.894802048023127	27.75807761271797	22.18259813742475
78-79	22.0992253701005	27.92273177167761	27.73184140901406	22.24620144920783
80-81	22.114326861155078	27.963787146992225	27.722675171689286	22.19921082016341
82-83	22.091457174619173	27.923625625568548	27.813911432273514	22.171005767538762
84-85	22.125618441692463	27.952047015651875	27.769048866659823	22.15328567599584
86-87	22.046868218146574	27.947452805665474	27.86422019794576	22.1414587782422
88-89	22.076185719015488	27.90676317009007	27.830666638971284	22.18638447192316
90-91	22.079203718166823	27.946839141499268	27.860775874229056	22.113181266104856
92-93	22.07257239282627	27.942901732577436	27.837059764036354	22.14746611055994
94-95	21.94332688411415	27.95235135285243	27.923417360851694	22.180904402181728
96-97	22.02016179626031	28.082312639920453	27.94788913697146	21.94963642684777
98-99	23.020946528683204	30.065006250118252	28.13831061036385	18.775736610834695
100	22.074621931155406	30.99516517899873	22.002094737227747	24.928118152618115
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6447.0
1	6531.5
2	10566.5
3	14707.5
4	18031.5
5	30737.0
6	42702.5
7	50635.0
8	55124.0
9	51528.5
10	42287.5
11	32074.5
12	31962.5
13	44686.5
14	80765.5
15	134599.0
16	178416.5
17	200942.0
18	204659.0
19	194670.5
20	176803.5
21	158564.5
22	149464.5
23	158763.0
24	189108.5
25	240153.0
26	307499.5
27	381303.0
28	459238.0
29	543806.5
30	626242.0
31	705111.5
32	791858.5
33	874200.5
34	945631.5
35	1010400.5
36	1077095.0
37	1141203.0
38	1191634.5
39	1226990.0
40	1259386.0
41	1289762.5
42	1307917.5
43	1325645.5
44	1339361.0
45	1356476.5
46	1362770.0
47	1350758.0
48	1337824.0
49	1309342.5
50	1274559.0
51	1236818.5
52	1182803.0
53	1116215.0
54	1046582.5
55	969714.5
56	888557.0
57	806707.5
58	719755.5
59	629113.5
60	528833.5
61	430590.0
62	342334.5
63	265301.0
64	199296.0
65	146344.5
66	109096.5
67	83018.0
68	62798.0
69	45901.5
70	33208.5
71	24630.0
72	17909.5
73	12624.0
74	8876.0
75	6146.0
76	4190.0
77	2811.5
78	1984.0
79	1462.0
80	1064.5
81	749.5
82	550.0
83	389.0
84	281.0
85	213.5
86	165.5
87	133.0
88	100.5
89	73.0
90	47.5
91	29.5
92	18.5
93	8.5
94	2.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.413082497331447E-4
24-25	1.560136498991585E-4
26-27	1.6050144538914024E-4
28-29	4.2582967275755845E-4
30-31	4.292834891241471E-4
32-33	4.4044016999394335E-4
34-35	9.48221112006752E-4
36-37	4.4147699234478897E-4
38-39	0.001996373528505101
40-41	7.506924424623895E-5
42-43	1.314027460636495E-4
44-45	9.129179857760987E-5
46-47	2.455582540531884E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.2752716987258137E-5
64-65	0.0
66-67	0.0
68-69	4.4411521885715215E-4
70-71	0.0
72-73	3.7067129553307576E-4
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.6148796834290806E-6
88-89	0.0
90-91	0.0
92-93	4.496638088533109E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1554.0
20-21	5477.0
22-23	9481.0
24-25	13678.0
26-27	19642.0
28-29	32845.0
30-31	60484.0
32-33	76447.0
34-35	102457.0
36-37	139073.0
38-39	180797.0
40-41	191102.0
42-43	228432.0
44-45	279914.0
46-47	271330.0
48-49	239428.0
50-51	213194.0
52-53	200271.0
54-55	210908.0
56-57	224087.0
58-59	232297.0
60-61	236341.0
62-63	222696.0
64-65	229113.0
66-67	246238.0
68-69	257543.0
70-71	262359.0
72-73	247077.0
74-75	243773.0
76-77	255319.0
78-79	276939.0
80-81	289987.0
82-83	281010.0
84-85	274943.0
86-87	289617.0
88-89	312108.0
90-91	353102.0
92-93	384231.0
94-95	470130.0
96-97	1196351.0
98-99	2182817.0
100-101	2.2546026E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.14203643463048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.21467406624988	67.36990202084691
2	8.884179752655777	13.884557958476682
3	2.428351338707996	5.692689563562118
4	1.0237158341639767	3.199809600477984
5	0.5073220398248436	1.982158866004199
6	0.2798940924828652	1.312289662358033
7	0.17680913655241698	0.9671358193318165
8	0.11748774697056745	0.7344585443517382
9	0.07633073594821366	0.5368175234592724
>10	0.28089711142270934	3.484052137776761
>50	0.007571530503765284	0.3999533064615134
>100	0.0026503306447526608	0.3475818136109482
>500	6.813493061515405E-5	0.035250698960698275
>1k	4.814894169999929E-5	0.05334248432129421
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.883976572594238E-6	2.941988286297119E-6
2	0.0	5.295578915334814E-5	0.0	2.059391800407983E-5	2.941988286297119E-6
3	2.941988286297119E-6	5.295578915334814E-5	0.0	2.647789457667407E-5	2.941988286297119E-6
4	2.941988286297119E-6	1.382734494559646E-4	0.0	3.530385943556542E-5	2.941988286297119E-6
5	2.941988286297119E-6	1.5004140260115305E-4	0.0	3.8245847721862546E-5	2.941988286297119E-6
6	5.883976572594238E-6	1.5004140260115305E-4	0.0	4.118783600815966E-5	1.1767953145188475E-5
7	5.883976572594238E-6	1.5298339088745018E-4	0.0	4.118783600815966E-5	1.1767953145188475E-5
8	5.883976572594238E-6	1.6475134403263865E-4	0.0	4.4129824294456785E-5	1.1767953145188475E-5
9	5.883976572594238E-6	2.941988286297119E-4	0.0	7.649169544372509E-5	1.1767953145188475E-5
10-11	5.883976572594238E-6	3.000828052023061E-4	0.0	9.41436251615078E-5	1.1767953145188475E-5
12-13	5.883976572594238E-6	3.074377759180489E-4	0.0	1.088535665929934E-4	1.3238947288337034E-5
14-15	8.825964858891356E-6	3.809874830754769E-4	0.0	1.2209251388133044E-4	2.6477894576674067E-5
16-17	1.4709941431485593E-5	3.9422643036381394E-4	0.0	1.4415742602855882E-4	3.530385943556542E-5
18-19	1.4709941431485593E-5	4.2658830151308223E-4	0.0	1.6475134403263865E-4	4.70718125807539E-5
20-21	1.4709941431485593E-5	4.618921609486476E-4	0.0	1.853452620367185E-4	4.70718125807539E-5
22-23	1.4709941431485593E-5	5.236739149608871E-4	0.0	1.9417122689560984E-4	5.44267832964967E-5
24-25	1.4709941431485593E-5	5.869266631162752E-4	0.0	2.3388806876062094E-4	6.766573058483373E-5
26-27	1.4709941431485593E-5	6.192885342655434E-4	0.0	2.824308754845234E-4	7.354970715742798E-5
28-29	1.6180935574634153E-5	6.237015166949893E-4	0.0	3.662775416439913E-4	7.796268958687366E-5
30-31	1.765192971778271E-5	6.987222179955658E-4	0.0	7.722719251529936E-4	8.384666615946789E-5
32-33	1.765192971778271E-5	7.090191769976057E-4	0.0	0.0016607523876147235	8.6788654445765E-5
34-35	1.765192971778271E-5	8.075757845885591E-4	0.0	0.0028654965908533935	9.41436251615078E-5
36-37	2.059391800407983E-5	9.164293511815524E-4	0.0	0.004767492017944481	9.708561344780492E-5
38-39	2.059391800407983E-5	9.649721579054549E-4	0.0	0.007946310361288518	9.855660759095348E-5
40-41	2.059391800407983E-5	0.0010488188240649228	0.0	0.011634092678161956	1.1473754316558763E-4
42-43	2.059391800407983E-5	0.001091477654216231	0.0	0.015060038037554952	1.1473754316558763E-4
44-45	2.059391800407983E-5	0.0011929762500934816	0.0	0.01885961590930768	1.1767953145188476E-4
46-47	2.059391800407983E-5	0.001262112974821464	0.0	0.02342411073549766	1.2797649045392467E-4
48-49	2.059391800407983E-5	0.001269467945537207	0.0	0.02814158895257509	1.5886736746004442E-4
50-51	2.059391800407983E-5	0.0014283353129972511	0.0	0.03306647734383647	1.6033836160319297E-4
52-53	2.2064912147228393E-5	0.0014415742602855882	0.0	0.03776189064876667	1.7651929717782714E-4
54-55	2.353590629037695E-5	0.0014489292310013312	0.0	0.042258719744371814	1.9711321518190697E-4
56-57	2.647789457667407E-5	0.001462168178289668	0.0	0.046914416207437006	2.0446818589764975E-4
58-59	2.647789457667407E-5	0.001472465137291708	0.0	0.05155981571150016	2.0741017418394688E-4
60-61	2.647789457667407E-5	0.0015136529732998677	0.0	0.05655972480406211	2.0888116832709543E-4
62-63	2.647789457667407E-5	0.00154160186201969	0.0	0.06126396407385121	2.206491214722839E-4
64-65	2.647789457667407E-5	0.0017166501650543687	0.0	0.06547836229397183	2.2359110975858103E-4
66-67	2.794888871982263E-5	0.0017357730889153002	0.0	0.06978396215096766	2.2947508633117526E-4
68-69	2.9419882862971187E-5	0.0017887288780686482	0.0	0.07425137136370984	2.324170746174724E-4
70-71	2.9419882862971187E-5	0.0018328587023631049	0.0	0.07804212327060367	2.353590629037695E-4
72-73	2.9419882862971187E-5	0.001853452620367185	0.0	0.08090909085560022	2.6330795162359216E-4
74-75	2.9419882862971187E-5	0.0019343572982403556	0.0	0.08312882101761139	2.647789457667407E-4
76-77	2.9419882862971187E-5	0.0020520368296922404	0.0	0.08527941445489459	2.647789457667407E-4
78-79	2.9419882862971187E-5	0.0021182315661339257	0.0	0.08676217655118834	2.647789457667407E-4
80-81	2.9419882862971187E-5	0.0021241155427065197	0.0	0.08728437947200607	2.7213391648248347E-4
82-83	2.9419882862971187E-5	0.002137354489994857	0.0	0.08769772882623081	2.8831485205711764E-4
84-85	2.9419882862971187E-5	0.0021579484079989365	0.0	0.08789190005312643	2.9125684034341475E-4
86-87	2.9419882862971187E-5	0.0022020782322933934	0.0	0.08798310169000163	2.9125684034341475E-4
88	2.9419882862971187E-5	0.002332996711033615	0.0	0.08799192765486052	2.941988286297119E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	44725	0.0	50.741974	1
GTATCAA	81295	0.0	37.110363	1
TCAACGC	102255	0.0	28.877262	4
ATCAACG	102990	0.0	28.692041	3
CAACGCA	104835	0.0	28.18299	5
TATCAAC	107785	0.0	27.615015	2
AACGCAG	108455	0.0	27.277967	6
ACGCAGA	125040	0.0	23.642696	7
CGCAGAG	126945	0.0	23.264202	8
GTACATG	103580	0.0	21.299978	1
TACATGG	104645	0.0	20.656034	2
ACATGGG	104820	0.0	20.055676	3
GCAGAGT	147680	0.0	19.834806	9
AGAGTAC	133505	0.0	18.959541	10-11
GAGTACT	88475	0.0	18.44121	12-13
CAGAGTA	143940	0.0	17.823948	10-11
CATGGGG	70120	0.0	17.573957	4
AGTACTT	95500	0.0	17.113926	12-13
GTGGTAT	20990	0.0	16.955153	1
GTACTTT	99370	0.0	16.40989	14-15
>>END_MODULE