FastQCFastQC Report
Fri 27 May 2016
SRR2139289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5212265
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75850.050.5934831
GTATCAA140650.038.898351
ATCAACG162850.033.0930443
TCAACGC167700.032.1356624
CAACGCA172550.031.2106465
AACGCAG172550.031.1979686
TATCAAC175000.030.868642
ACGCAGA177250.030.6198487
CGCAGAG198350.028.0240618
GCAGAGT219050.023.6993759
GAGTACT127550.022.85440412-13
GTACATG155350.022.699491
TACATGG153500.022.269662
CAGAGTA206450.021.587510-11
ACATGGG155700.021.4004943
AGAGTAC191850.021.10656510-11
GTGGTAT37150.020.822431
TGGTATC35200.020.6258622
GTACTTT145750.019.44840814-15
AGTACTT139600.018.99150512-13
Produced by FastQC (version 0.11.5)