##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139289_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5212265
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.762610304733165	33.0	33.0	33.0	33.0	33.0
2	32.81618221636851	33.0	33.0	33.0	33.0	33.0
3	33.47979889740832	33.0	33.0	33.0	33.0	37.0
4	36.617155497657926	37.0	37.0	37.0	37.0	37.0
5	36.631064422089054	37.0	37.0	37.0	37.0	37.0
6	36.58694406366522	37.0	37.0	37.0	37.0	37.0
7	36.57379737983391	37.0	37.0	37.0	37.0	37.0
8	36.564434847422376	37.0	37.0	37.0	37.0	37.0
9	36.58375063432116	37.0	37.0	37.0	37.0	37.0
10-11	36.53917442800778	37.0	37.0	37.0	37.0	37.0
12-13	36.62306924149098	37.0	37.0	37.0	37.0	38.5
14-15	35.153696137859455	40.0	37.0	40.0	17.5	40.0
16-17	36.11934571630567	40.0	37.0	40.0	24.0	40.0
18-19	38.11315013722441	40.0	37.0	40.0	35.0	40.0
20-21	38.39936340259496	40.0	37.0	40.0	37.0	40.0
22-23	38.43912240705492	40.0	37.0	40.0	37.0	40.0
24-25	38.46308178796107	40.0	37.0	40.0	37.0	40.0
26-27	38.40130728447058	40.0	37.0	40.0	37.0	40.0
28-29	38.33345682238942	40.0	37.0	40.0	37.0	40.0
30-31	38.20376474733361	40.0	37.0	40.0	37.0	40.0
32-33	38.02198704475279	40.0	37.0	40.0	33.0	40.0
34-35	37.947503072907416	40.0	37.0	40.0	33.0	40.0
36-37	37.891626365180585	40.0	37.0	40.0	33.0	40.0
38-39	37.885042572923155	40.0	37.0	40.0	33.0	40.0
40-41	37.834882296697046	40.0	37.0	40.0	33.0	40.0
42-43	37.78511108799094	40.0	37.0	40.0	33.0	40.0
44-45	37.73318180931706	40.0	37.0	40.0	33.0	40.0
46-47	37.582484304784984	40.0	37.0	40.0	33.0	40.0
48-49	37.37455390173006	40.0	37.0	40.0	33.0	40.0
50-51	37.28171889542121	40.0	37.0	40.0	33.0	40.0
52-53	37.19634401731463	40.0	37.0	40.0	33.0	40.0
54-55	36.981976659357606	37.0	37.0	40.0	33.0	40.0
56-57	36.88619024797339	37.0	37.0	40.0	33.0	40.0
58-59	36.82884692961252	37.0	37.0	40.0	33.0	40.0
60-61	36.70252850803701	37.0	37.0	40.0	33.0	40.0
62-63	36.51903211196823	37.0	37.0	40.0	33.0	40.0
64-65	36.23437990624886	37.0	37.0	40.0	33.0	40.0
66-67	35.90669162275984	37.0	35.0	40.0	33.0	40.0
68-69	35.62545909576491	37.0	33.0	38.5	33.0	40.0
70-71	35.377825899475795	37.0	33.0	37.0	30.0	40.0
72-73	35.13900426752515	37.0	33.0	37.0	27.0	40.0
74-75	34.499398027245604	37.0	33.0	37.0	27.0	38.5
76-77	32.35838206297705	33.0	30.0	37.0	24.5	37.0
78-79	34.21768314333802	37.0	33.0	37.0	27.0	37.0
80-81	34.490916781130785	37.0	33.0	37.0	27.0	37.0
82-83	34.4551439695878	37.0	33.0	37.0	27.0	37.0
84-85	34.39988378069817	37.0	33.0	37.0	27.0	37.0
86-87	34.209994612026954	37.0	33.0	37.0	27.0	37.0
88-89	33.98887683493295	37.0	33.0	37.0	27.0	37.0
90-91	33.853529035594164	37.0	33.0	37.0	27.0	37.0
92-93	33.86155037380131	37.0	33.0	37.0	27.0	37.0
94-95	33.79985761131839	35.0	33.0	37.0	27.0	37.0
96-97	33.74851173454459	33.0	33.0	37.0	27.0	37.0
98-99	33.72940716634781	33.0	33.0	37.0	27.0	37.0
100	33.69914281924831	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	1.0
18	0.0
19	6.0
20	41.0
21	192.0
22	772.0
23	2205.0
24	5411.0
25	10988.0
26	19487.0
27	31647.0
28	47885.0
29	67731.0
30	92594.0
31	124277.0
32	167892.0
33	227586.0
34	323706.0
35	501226.0
36	891414.0
37	1605391.0
38	1066021.0
39	25789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.88389523312161	0.0	24.51797282482947	29.598131942048916
2	14.935706784103644	23.97800627526038	38.60564819479784	22.480638745838142
3	16.89489744723672	24.490929445272098	32.70735266044344	25.90682044704774
4	13.126737854134696	23.338993719982955	37.21103393603565	26.323234489846705
5	17.55966827275982	36.18722797172959	32.077934336793014	14.175169418717578
6	28.018799544304635	37.192853609973724	20.353094841502354	14.435252004219286
7	26.141990911768175	32.93638909220325	22.33844022765666	18.58317976837192
8	24.087005587065928	32.339079512614305	22.174959331125315	21.398955569194452
9	23.84008696589781	20.678939510275782	23.699300750284237	31.78167277354217
10-11	24.738757820236984	26.209686200474554	28.36290216665642	20.688653812632047
12-13	25.12618043444006	24.182448173944458	28.408520518963815	22.282850872651665
14-15	22.652867392348686	26.39785434899171	26.625545669145367	24.32373258951424
16-17	21.637179003483034	28.861054537811732	27.449259338239646	22.052507120465588
18-19	21.596733857545615	27.85700650293107	29.3810752139425	21.16518442558082
20-21	22.439753054821303	27.172699499444263	29.295624507027718	21.09192293870672
22-23	22.21426833443095	27.00787214216524	29.09653045465919	21.681329068744617
24-25	21.926838742484918	27.2210414753612	29.31357894723708	21.53854083491681
26-27	21.906466453038366	27.483112089168397	29.067557213566264	21.542864244226976
28-29	21.839573923092424	27.38686980311844	29.105255981773603	21.668300292015534
30-31	22.117267456283482	27.227476533551854	29.130494546322712	21.524761463841955
32-33	21.89436492091951	27.541882448676468	28.975907276819235	21.587845353584783
34-35	22.042937735312698	27.39180985117113	28.929616496708217	21.635635916807956
36-37	22.057590318906332	27.406626731832834	28.92568203198495	21.610100917275886
38-39	21.86077852221458	27.619791386412146	28.917544938888152	21.60188515248512
40-41	22.247980321296108	27.364810719602477	28.60884202366261	21.778366935438804
42-43	22.12649684777683	27.441296034430717	28.61066631516304	21.821540802629414
44-45	22.14658734172804	27.810103309120098	28.250763334008415	21.792546015143444
46-47	22.267871209646025	27.60784843827829	27.87948942788144	22.24479092419425
48-49	22.154914930127656	27.745691141982697	27.99801439533721	22.101379532552436
50-51	22.152990470231085	27.99645561840267	27.77651496803426	22.07403894333198
52-53	22.397862436480573	27.787827736731312	27.66697922138615	22.147330605401958
54-55	22.32383724128275	27.743990558914277	27.747090915455274	22.1850812843477
56-57	22.345201918496667	28.029340459967077	27.485543211115264	22.139914410420992
58-59	22.325639859340058	27.88627635225013	27.669374709646675	22.118709078763136
60-61	22.30178292307888	27.951567523604986	27.660799837338452	22.085849715977684
62-63	22.23114429230338	28.169356888961882	27.507956743458966	22.091542075275775
64-65	22.351019628208512	27.992755094434578	27.432399207804664	22.223826069552246
66-67	22.30716544058802	27.963884898965436	27.41787628904553	22.311073371401015
68-69	22.360870210566453	28.031557432292452	27.474802254671754	22.132770102469344
70-71	22.378897511121025	27.646539336210857	27.50337845515415	22.471184697513973
72-73	22.33446597483699	27.79905774130548	27.496765794347194	22.369710489510343
74-75	22.22026284944163	27.905707549283466	27.46458067433345	22.409448926941455
76-77	22.42081224816222	27.762228084880764	27.30987480099556	22.507084865961456
78-79	22.299285619696047	27.797097989023683	27.461957014541365	22.4416593767389
80-81	22.310918394335598	27.921227018009564	27.321667774364478	22.44618681329036
82-83	22.393346459537135	27.70672636998437	27.467184398307893	22.432742772170606
84-85	22.506812226966716	27.75603503376651	27.475420638996795	22.26173210026998
86-87	22.24798110811848	27.842470326148828	27.475461931394285	22.434086634338403
88-89	22.392927877547127	27.711158444556073	27.39872705474216	22.49718662315464
90-91	22.469253739429	27.709965178990082	27.491645012506794	22.329136069074124
92-93	22.36038165027165	27.756875380224912	27.537623722189135	22.345119247314305
94-95	22.42108712193646	27.657924634214577	27.531566300267414	22.389421943581556
96-97	22.31744709864793	27.99908226724333	27.690443559622302	21.99302707448644
98-99	23.469243690379184	30.005313832974444	27.24546962388512	19.279972852761247
100	22.619596003388505	30.38224799413572	21.53754880960191	25.46060719287387
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	763.0
1	738.5
2	1259.5
3	1973.5
4	2903.0
5	5077.5
6	6815.0
7	7736.0
8	8247.0
9	7963.5
10	7361.0
11	6822.0
12	7402.0
13	9700.0
14	15461.0
15	24141.0
16	32342.0
17	37938.0
18	38559.5
19	34997.0
20	30256.5
21	25652.0
22	22721.0
23	22684.5
24	25494.5
25	30884.0
26	38773.5
27	48108.0
28	57776.0
29	68538.5
30	79433.0
31	89758.0
32	101041.0
33	112998.5
34	125408.5
35	136492.5
36	148171.0
37	159137.0
38	166163.5
39	172162.5
40	179197.5
41	186548.5
42	191008.5
43	194575.0
44	197991.0
45	201566.5
46	204092.0
47	205141.5
48	206817.0
49	205347.5
50	201267.0
51	195756.5
52	186473.5
53	176293.0
54	166653.0
55	154537.0
56	140966.5
57	126779.5
58	111800.0
59	97596.0
60	83026.5
61	67772.0
62	53711.0
63	41511.5
64	31214.0
65	23365.5
66	17690.0
67	13692.5
68	10533.0
69	7626.0
70	5568.0
71	4120.0
72	3009.5
73	2157.0
74	1545.5
75	1113.0
76	804.5
77	563.5
78	396.5
79	288.0
80	194.5
81	138.0
82	105.0
83	71.0
84	49.5
85	38.5
86	30.5
87	24.5
88	18.0
89	12.5
90	7.5
91	5.5
92	6.0
93	4.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.3429862065723826E-4
2	9.784613790741646E-4
3	0.0013429862065723827
4	3.453393102614698E-4
5	5.94751034339198E-4
6	2.1104068960423153E-4
7	5.755655171024497E-4
8	9.209048273639195E-4
9	0.002052850344332071
10-11	0.007415202412003227
12-13	0.22022095960201565
14-15	6.807242149046528
16-17	9.592758618374163E-6
18-19	0.0
20-21	1.9186463259457775E-5
22-23	3.3580836893941455E-4
24-25	0.011506998347176601
26-27	0.022573582341102287
28-29	0.007330838734422448
30-31	1.5390491888777916E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.695290590158156E-6
40-41	3.887687431485584E-5
42-43	5.849724097762929E-5
44-45	1.761932221012471E-4
46-47	0.0019282390111282019
48-49	0.031893560307688355
50-51	0.07256603251069346
52-53	0.052403628194665146
54-55	0.03417224973376039
56-57	0.004363136842046002
58-59	0.011678499700049616
60-61	0.03484825510752043
62-63	0.02843186458539829
64-65	2.0591435445438047E-4
66-67	0.006547434081369017
68-69	0.04170042964968703
70-71	0.08599797144905368
72-73	0.07311717686881325
74-75	0.03499917699424582
76-77	0.007161864400737747
78-79	8.371659042114772E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	1.0345399555998441E-4
90-91	0.002054845699768363
92-93	0.0032873613062858265
94-95	0.0010038897704841895
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	141.0
20-21	561.0
22-23	1118.0
24-25	2310.0
26-27	3020.0
28-29	5084.0
30-31	8309.0
32-33	9052.0
34-35	11142.0
36-37	11473.0
38-39	12119.0
40-41	14946.0
42-43	19138.0
44-45	24904.0
46-47	24847.0
48-49	18881.0
50-51	17781.0
52-53	19934.0
54-55	26416.0
56-57	28508.0
58-59	26686.0
60-61	28284.0
62-63	31868.0
64-65	41926.0
66-67	44617.0
68-69	35476.0
70-71	34241.0
72-73	34186.0
74-75	39412.0
76-77	27489.0
78-79	34810.0
80-81	41853.0
82-83	50671.0
84-85	55235.0
86-87	59847.0
88-89	66239.0
90-91	68648.0
92-93	75436.0
94-95	98105.0
96-97	284763.0
98-99	968013.0
100-101	2804776.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.22274493327414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.24676663261894	80.50040218615531
2	6.525295195531275	11.513589072993497
3	1.3912872844371733	3.682295496714251
4	0.4390952483707152	1.5495275239368902
5	0.1760127666927258	0.7764164710466118
6	0.07804508403062586	0.41312109250379114
7	0.04444398118815166	0.2744679011327077
8	0.02689218437018569	0.18980018579115787
9	0.01712370871925877	0.13596305279857637
>10	0.05312048092663476	0.7029935046892193
>50	0.0010468841266471228	0.06632359932385892
>100	8.398824502218325E-4	0.17935398376516182
>500	3.0666537411827964E-5	0.01574592914896977
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9185517236748322E-5	0.0	5.755655171024497E-5	0.0
3	0.0	1.9185517236748322E-5	0.0	5.755655171024497E-5	0.0
4	0.0	1.9185517236748322E-5	0.0	5.755655171024497E-5	0.0
5	0.0	1.9185517236748322E-5	0.0	5.755655171024497E-5	0.0
6	0.0	1.9185517236748322E-5	0.0	5.755655171024497E-5	1.9185517236748322E-5
7	0.0	1.9185517236748322E-5	0.0	7.674206894699329E-5	1.9185517236748322E-5
8	0.0	1.9185517236748322E-5	0.0	9.592758618374162E-5	1.9185517236748322E-5
9	0.0	5.755655171024497E-5	0.0	9.592758618374162E-5	1.9185517236748322E-5
10-11	0.0	5.755655171024497E-5	0.0	9.592758618374162E-5	1.9185517236748322E-5
12-13	0.0	5.755655171024497E-5	0.0	9.592758618374162E-5	1.9185517236748322E-5
14-15	0.0	1.0552034480211578E-4	0.0	9.592758618374162E-5	1.9185517236748322E-5
16-17	0.0	1.1511310342048994E-4	0.0	1.1511310342048994E-4	1.9185517236748322E-5
18-19	0.0	1.1511310342048994E-4	0.0	2.5900448269610234E-4	3.8371034473496644E-5
20-21	0.0	1.1511310342048994E-4	0.0	5.084162067738305E-4	4.796379309187081E-5
22-23	0.0	1.3429862065723826E-4	0.0	7.098641377596879E-4	6.714931032861913E-5
24-25	0.0	1.4389137927561243E-4	0.0	0.0010456106894027838	8.633482756536745E-5
26-27	0.0	1.5348413789398658E-4	0.0	0.002426967930448663	9.592758618374162E-5
28-29	0.0	1.5348413789398658E-4	0.0	0.00675330206733541	9.592758618374162E-5
30-31	0.0	1.7266965513073492E-4	0.0	0.01099330137665679	9.592758618374162E-5
32-33	0.0	1.7266965513073492E-4	0.0	0.019079996891946206	1.1511310342048994E-4
34-35	0.0	2.110406896042316E-4	0.0	0.028931759993016472	1.1511310342048994E-4
36-37	0.0	2.685972413144765E-4	0.0	0.04033754999026335	1.1511310342048994E-4
38-39	0.0	2.781899999328507E-4	0.0	0.0526834303321109	1.1511310342048994E-4
40-41	0.0	3.261537930247215E-4	0.0	0.06270786308831189	1.1511310342048994E-4
42-43	0.0	3.741175861165923E-4	0.0	0.07131256756899351	1.2470586203886408E-4
44-45	0.0	4.220813792084631E-4	0.0	0.07984052998072814	1.3429862065723826E-4
46-47	0.0	4.220813792084631E-4	0.0	0.08964432928870654	1.3429862065723826E-4
48-49	0.0	4.220813792084631E-4	0.0	0.09933301549326444	1.3429862065723826E-4
50-51	0.0	4.988234481554563E-4	0.0	0.10946296859426756	1.3429862065723826E-4
52-53	0.0	5.180089653922047E-4	0.0	0.13623635789814986	1.3429862065723826E-4
54-55	0.0	5.467872412473272E-4	0.0	0.16615617202885885	1.3429862065723826E-4
56-57	0.0	5.947510343391981E-4	0.0	0.1992128182277762	1.3429862065723826E-4
58-59	0.0	6.139365515759463E-4	0.0	0.21900267925748212	1.3429862065723826E-4
60-61	0.0	6.331220688126947E-4	0.0	0.23300810684030837	1.3429862065723826E-4
62-63	0.0	6.52307586049443E-4	0.0	0.24627489200951985	1.3429862065723826E-4
64-65	0.0	6.906786205229397E-4	0.0	0.2510233075256151	1.4389137927561243E-4
66-67	0.0	7.290496549964363E-4	0.0	0.25581968683480216	1.6307689651236075E-4
68-69	0.0	7.386424136148104E-4	0.0	0.26143145062655104	1.7266965513073492E-4
70-71	0.0	7.578279308515588E-4	0.0	0.2650862916601516	1.7266965513073492E-4
72-73	0.0	7.770134480883071E-4	0.0	0.26781063510776987	2.1104068960423156E-4
74-75	0.0	7.961989653250555E-4	0.0	0.27058294234848	2.1104068960423156E-4
76-77	0.0	8.441627584169262E-4	0.0	0.27271253476175905	2.1104068960423156E-4
78-79	0.0	8.729410342720488E-4	0.0	0.27397877889938443	2.206334482226057E-4
80-81	0.0	9.113120687455453E-4	0.0	0.2744200457958296	2.3981896545935405E-4
82-83	0.0	9.209048273639195E-4	0.0	0.27501479683016883	2.494117240777282E-4
84-85	0.0	9.209048273639195E-4	0.0	0.27552321303694266	2.5900448269610234E-4
86-87	0.0	9.209048273639195E-4	0.0	0.275839774071349	2.685972413144765E-4
88	0.0	9.592758618374162E-4	0.0	0.27586855234720414	2.685972413144765E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7585	0.0	50.593483	1
GTATCAA	14065	0.0	38.89835	1
ATCAACG	16285	0.0	33.093044	3
TCAACGC	16770	0.0	32.135662	4
CAACGCA	17255	0.0	31.210646	5
AACGCAG	17255	0.0	31.197968	6
TATCAAC	17500	0.0	30.86864	2
ACGCAGA	17725	0.0	30.619848	7
CGCAGAG	19835	0.0	28.024061	8
GCAGAGT	21905	0.0	23.699375	9
GAGTACT	12755	0.0	22.854404	12-13
GTACATG	15535	0.0	22.69949	1
TACATGG	15350	0.0	22.26966	2
CAGAGTA	20645	0.0	21.5875	10-11
ACATGGG	15570	0.0	21.400494	3
AGAGTAC	19185	0.0	21.106565	10-11
GTGGTAT	3715	0.0	20.82243	1
TGGTATC	3520	0.0	20.625862	2
GTACTTT	14575	0.0	19.448408	14-15
AGTACTT	13960	0.0	18.991505	12-13
>>END_MODULE