FastQCFastQC Report
Fri 27 May 2016
SRR2139288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048353
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54300.051.771161
GTATCAA106000.039.1400761
CTTATAC37250.031.9329171
TCAACGC135400.030.2462434
ATCAACG135850.030.1763523
TATCAAC136600.030.070932
CAACGCA137450.029.7651925
AACGCAG137950.029.6871416
TATACAC48750.027.2706153
CGCAGAG150800.027.1587728
ACGCAGA156350.026.1934227
GCAGAGT160700.024.5900549
GAGTACT100000.023.81799312-13
TTATACA52600.023.3183352
GTACATG98400.022.587371
AGAGTAC142300.022.20188710-11
TACATGG99200.022.0732862
CAGAGTA151650.021.57938210-11
AGTACTT105600.021.25008812-13
TACACAT62600.021.039855
Produced by FastQC (version 0.11.5)