##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139288_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3048353
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62348291027975	33.0	33.0	33.0	33.0	33.0
2	32.647442077738376	33.0	33.0	33.0	33.0	33.0
3	32.63273708786351	33.0	33.0	33.0	33.0	33.0
4	36.55651494429943	37.0	37.0	37.0	37.0	37.0
5	36.579609710555175	37.0	37.0	37.0	37.0	37.0
6	36.54210158731617	37.0	37.0	37.0	37.0	37.0
7	36.50792181876574	37.0	37.0	37.0	37.0	37.0
8	36.521799148589416	37.0	37.0	37.0	37.0	37.0
9	36.53202926301514	37.0	37.0	37.0	37.0	37.0
10-11	36.4753816241098	37.0	37.0	37.0	37.0	37.0
12-13	36.4427891061173	37.0	37.0	37.0	37.0	37.0
14-15	34.81921237468233	40.0	35.0	40.0	19.5	40.0
16-17	35.081106420417846	40.0	33.0	40.0	24.0	40.0
18-19	37.85520344920683	40.0	37.0	40.0	35.0	40.0
20-21	38.25934452823997	40.0	37.0	40.0	37.0	40.0
22-23	38.309020736268735	40.0	37.0	40.0	37.0	40.0
24-25	38.35282759602572	40.0	37.0	40.0	37.0	40.0
26-27	38.27840197165183	40.0	37.0	40.0	37.0	40.0
28-29	38.195525967023414	40.0	37.0	40.0	37.0	40.0
30-31	38.05553622651164	40.0	37.0	40.0	35.0	40.0
32-33	37.84115323398345	40.0	37.0	40.0	33.0	40.0
34-35	37.73942101910837	40.0	37.0	40.0	33.0	40.0
36-37	37.65828275562549	40.0	37.0	40.0	33.0	40.0
38-39	37.6687229310549	40.0	37.0	40.0	33.0	40.0
40-41	37.631240614364444	40.0	37.0	40.0	33.0	40.0
42-43	37.60813109987825	40.0	37.0	40.0	33.0	40.0
44-45	37.56014389541035	40.0	37.0	40.0	33.0	40.0
46-47	37.41209954259702	40.0	37.0	40.0	33.0	40.0
48-49	37.1938478738347	40.0	37.0	40.0	33.0	40.0
50-51	37.111373694945755	40.0	37.0	40.0	33.0	40.0
52-53	37.05890906954683	40.0	37.0	40.0	33.0	40.0
54-55	36.84039576649871	37.0	37.0	40.0	33.0	40.0
56-57	36.74820594509781	37.0	37.0	40.0	33.0	40.0
58-59	36.71929415103972	37.0	37.0	40.0	33.0	40.0
60-61	36.61531062228484	37.0	37.0	40.0	33.0	40.0
62-63	36.454685584650605	37.0	37.0	40.0	33.0	40.0
64-65	36.20140871620547	37.0	37.0	40.0	33.0	40.0
66-67	35.87733464514906	37.0	35.0	40.0	33.0	40.0
68-69	35.59730226971499	37.0	33.0	38.5	33.0	40.0
70-71	35.37346231066514	37.0	33.0	37.0	30.0	40.0
72-73	35.168439277261015	37.0	33.0	37.0	27.0	40.0
74-75	34.88004341661358	37.0	33.0	37.0	27.0	40.0
76-77	31.887999574546463	33.0	30.0	35.0	24.5	37.0
78-79	34.06873087726279	37.0	33.0	37.0	27.0	37.0
80-81	34.40912647289614	37.0	33.0	37.0	27.0	37.0
82-83	34.3925942220458	37.0	33.0	37.0	27.0	37.0
84-85	34.352671327146936	37.0	33.0	37.0	27.0	37.0
86-87	34.16395956633083	37.0	33.0	37.0	27.0	37.0
88-89	33.92962920232486	37.0	33.0	37.0	27.0	37.0
90-91	33.7754072926424	37.0	33.0	37.0	27.0	37.0
92-93	33.79949674365666	37.0	33.0	37.0	27.0	37.0
94-95	33.72779915428828	33.0	33.0	37.0	27.0	37.0
96-97	33.67692638987909	33.0	33.0	37.0	27.0	37.0
98-99	33.67566608164401	33.0	33.0	37.0	27.0	37.0
100	33.690071697027776	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	9.0
20	40.0
21	171.0
22	638.0
23	1863.0
24	3948.0
25	7693.0
26	13314.0
27	21099.0
28	31398.0
29	43787.0
30	59170.0
31	78073.0
32	103226.0
33	140102.0
34	194020.0
35	298540.0
36	519582.0
37	906366.0
38	605658.0
39	19654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44459512744961	20.598213717489706	14.80066869137387	23.15652246368682
2	14.4172985669273	21.32983635382478	42.00535044425745	22.247514634990463
3	17.481010062903398	27.10025997884084	31.509435509665146	23.90929444859062
4	12.122802655086149	18.828719124978925	38.91917497337894	30.129303246555995
5	12.76644742237604	38.05521019567963	35.66644250168124	13.511899880263092
6	28.718688419615447	38.59674388104003	18.87635716729657	13.808210532047962
7	25.236578572101067	33.76311732925944	23.104607635664244	17.895696462975252
8	24.22491752103513	36.40952999865829	21.557017838813287	17.808534641493292
9	25.266397953255414	16.888988906468512	21.402475369486407	36.44213777078967
10-11	23.718857363304053	27.19773595774505	28.879152119193545	20.204254559757352
12-13	24.856045215235902	24.909467505895808	29.05445990014936	21.180027378718936
14-15	22.41597497594184	25.820718049066148	27.091578126087974	24.67172884890404
16-17	21.206746725198823	29.434402774219393	27.596885924956855	21.761964575624937
18-19	21.104478385541306	27.93751248625077	30.087263515741125	20.8707456124668
20-21	22.007427699996505	27.07514915845809	30.103148283839122	20.814274857706284
22-23	21.6232435767683	27.30745234162148	29.969076746727417	21.100227334882803
24-25	21.45788521993996	27.490164485519863	30.143077846324356	20.90887244821582
26-27	21.455911693268565	27.662424032132567	30.082914064307193	20.798750210291676
28-29	21.08602979537323	27.53869605328948	30.13569165552304	21.239582495814254
30-31	21.582145210966857	27.31782260951822	30.123237331662768	20.976794847852158
32-33	21.382356558611846	27.60808714824265	30.001593128251773	21.007963164893727
34-35	21.255539111494322	27.796056415801946	30.096201896362025	20.852202576341703
36-37	21.455270036159348	27.710879981457087	29.752626110242	21.081223872141564
38-39	21.308054119640698	27.63917594684117	29.83999710622247	21.212772827295655
40-41	21.477686730574028	27.505189288343413	29.82012143202904	21.197002549053526
42-43	21.69648668841996	27.683172997775422	29.453851641416804	21.16648867238781
44-45	21.51943480346391	28.066187973039725	29.058135751173758	21.356241472322605
46-47	21.938620055692727	27.778286458018105	28.425862269048395	21.857231217240777
48-49	21.736071690476003	27.97573113106776	28.449598410908784	21.838598767547452
50-51	21.617819897615327	28.277429350812533	28.360628764672402	21.74412198689974
52-53	21.75993649452692	28.26292738774436	28.34475229200904	21.632383825719682
54-55	21.775101625744536	28.229452704924828	28.17790231629158	21.817543353039056
56-57	22.021242247008423	28.42199035903579	27.822657976629245	21.734109417326543
58-59	21.631147066543235	28.63481764162184	28.17150901298553	21.562526278849397
60-61	21.93464011830223	28.749619344116788	27.826060203779164	21.489680333801818
62-63	21.67980943728504	28.860148091128185	27.76923099873868	21.690811472848097
64-65	21.735589265935406	28.767957224519897	27.794995625441043	21.701457884103654
66-67	21.906608137668304	28.57150801660755	27.641604936164565	21.880278909559582
68-69	21.883609461918418	28.435404510105627	27.77406943152576	21.906916596450195
70-71	21.87311634848638	28.166684476182773	27.856188846205086	22.10401032912576
72-73	21.950858878562993	28.27653516817262	27.778263577562996	21.99434237570139
74-75	21.736469562973344	28.3264388644647	27.81812671164184	22.11896486092012
76-77	21.921434059565822	28.26340686817122	27.715721001705973	22.09943807055698
78-79	21.95256403764346	28.333417730169728	27.65058556995242	22.06343266223439
80-81	21.99188817199985	28.29559213584992	27.6077883917492	22.104731300401035
82-83	21.9507304954059	28.200896601652236	27.68428225623842	22.164090646703443
84-85	22.130626675918837	28.17546362802802	27.70427400576132	21.989635690291824
86-87	21.895229425910827	28.182200564518777	27.74856615009177	22.174003859478628
88-89	21.951998627967818	28.115862191578973	27.781047727953318	22.151091452499898
90-91	22.05492433110091	28.03950619765295	27.903107813877874	22.002461657368265
92-93	22.00886943339759	28.04007949081952	27.823392095307092	22.1276589804758
94-95	21.925530537610655	28.05920689274274	27.887482198617697	22.12778037102891
96-97	21.90797386692909	28.21069300280388	28.002403602083774	21.87892952818326
98-99	23.01597900920706	30.178929822498997	28.15318395280217	18.65190721549177
100	21.919318275480983	31.052208691225065	22.155934727501393	24.87253830579256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	822.0
1	789.0
2	1222.0
3	1804.5
4	2634.5
5	4536.0
6	6066.0
7	6958.5
8	7482.5
9	7296.5
10	6750.0
11	6239.0
12	6857.0
13	9195.0
14	14512.0
15	21829.5
16	28190.0
17	32499.5
18	33122.0
19	30187.0
20	26194.5
21	22331.5
22	19794.0
23	19534.0
24	21155.0
25	24871.5
26	30742.0
27	37044.5
28	42917.0
29	49723.0
30	56260.0
31	62033.5
32	68806.5
33	75800.5
34	82658.5
35	89569.0
36	96855.0
37	102655.5
38	105670.0
39	107711.5
40	111046.0
41	113944.0
42	114904.0
43	116074.5
44	116839.5
45	117481.0
46	119566.0
47	121415.5
48	120767.0
49	118527.0
50	116318.0
51	113300.0
52	108049.0
53	101753.5
54	95457.0
55	88491.5
56	80688.0
57	72048.0
58	63574.0
59	55885.0
60	47422.0
61	38638.5
62	30498.0
63	23311.5
64	17688.5
65	13604.0
66	10585.5
67	8273.0
68	6371.5
69	4528.5
70	3187.0
71	2419.5
72	1808.5
73	1307.0
74	924.0
75	663.5
76	491.5
77	356.5
78	248.5
79	171.5
80	124.5
81	101.5
82	83.5
83	63.0
84	39.0
85	20.5
86	12.5
87	8.5
88	5.0
89	3.0
90	2.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	3.608505970273128E-4
2	2.6243679783804564E-4
3	9.185287924331598E-4
4	4.920689959463356E-4
5	9.841379918926711E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	8.130554433820492
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.00960150787988366
26-27	0.025885460123908573
28-29	0.009288919256439276
30-31	8.23426008832726E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.024513987531342075
50-51	0.07165164687243811
52-53	0.06270134891053752
54-55	0.039039698259303424
56-57	0.0048438375582160855
58-59	0.010009988148174031
60-61	0.038598754711359214
62-63	0.03951711575756175
64-65	0.0
66-67	0.0037776125258728106
68-69	0.03765077081979692
70-71	0.09814127271065133
72-73	0.08820079122582818
74-75	0.04132560984405358
76-77	0.01015260442787836
78-79	0.0010292191191037066
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0013475211321004655
92-93	0.0029080026604734743
94-95	0.0010452078526465968
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	88.0
20-21	386.0
22-23	869.0
24-25	2194.0
26-27	2620.0
28-29	4311.0
30-31	7440.0
32-33	7545.0
34-35	9203.0
36-37	10839.0
38-39	14833.0
40-41	21517.0
42-43	22874.0
44-45	35090.0
46-47	33104.0
48-49	24630.0
50-51	20847.0
52-53	16878.0
54-55	27867.0
56-57	28524.0
58-59	32597.0
60-61	33685.0
62-63	33031.0
64-65	37248.0
66-67	39566.0
68-69	29418.0
70-71	27413.0
72-73	27290.0
74-75	31596.0
76-77	26527.0
78-79	36732.0
80-81	39221.0
82-83	42485.0
84-85	43143.0
86-87	40181.0
88-89	37132.0
90-91	39989.0
92-93	42814.0
94-95	49923.0
96-97	106494.0
98-99	176732.0
100-101	1783477.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.44417150673873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.65941289595168	85.64607416077862
2	4.8960968867729795	8.954390468553559
3	0.9193163588080582	2.521983683513839
4	0.2730485464875635	0.9987479245869793
5	0.11518286515580813	0.5266400837972638
6	0.04799405492427027	0.2633265953879285
7	0.026882503634275162	0.17207737910042295
8	0.017887909599805674	0.13085960586733347
9	0.012525245984264842	0.10308246677542844
>10	0.029763742701249662	0.41722906970520224
>50	0.00101723919121965	0.0668194680243948
>100	8.259309127267158E-4	0.17589190902952642
>500	4.581987601411715E-5	0.022877184879562282
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2804599729755704E-5	0.0	0.0	0.0	0.0
2	3.2804599729755704E-5	3.2804599729755704E-5	0.0	6.560919945951141E-5	0.0
3	3.2804599729755704E-5	3.2804599729755704E-5	0.0	6.560919945951141E-5	0.0
4	3.2804599729755704E-5	1.6402299864877855E-4	0.0	9.841379918926713E-5	0.0
5	3.2804599729755704E-5	1.9682759837853425E-4	0.0	9.841379918926713E-5	0.0
6	6.560919945951141E-5	1.9682759837853425E-4	0.0	1.3121839891902282E-4	0.0
7	6.560919945951141E-5	1.9682759837853425E-4	0.0	1.3121839891902282E-4	0.0
8	6.560919945951141E-5	1.9682759837853425E-4	0.0	1.3121839891902282E-4	0.0
9	6.560919945951141E-5	2.2963219810828996E-4	0.0	1.6402299864877855E-4	0.0
10-11	6.560919945951141E-5	2.2963219810828996E-4	0.0	2.132298982434121E-4	0.0
12-13	6.560919945951141E-5	2.2963219810828996E-4	0.0	2.2963219810828996E-4	0.0
14-15	9.841379918926713E-5	4.264597964868242E-4	0.0	2.2963219810828996E-4	0.0
16-17	1.9682759837853425E-4	4.592643962165799E-4	0.0	2.460344979731678E-4	0.0
18-19	1.9682759837853425E-4	4.920689959463356E-4	0.0	2.7883909770292346E-4	0.0
20-21	1.9682759837853425E-4	5.248735956760913E-4	0.0	3.444482971624349E-4	0.0
22-23	2.2963219810828993E-4	5.412758955409692E-4	0.0	8.037126933790148E-4	0.0
24-25	2.6243679783804564E-4	5.740804952707249E-4	0.0	0.002001080583515098	0.0
26-27	2.6243679783804564E-4	5.904827951356027E-4	0.0	0.004264597964868242	0.0
28-29	2.6243679783804564E-4	5.904827951356027E-4	0.0	0.012580563996361314	0.0
30-31	2.6243679783804564E-4	6.888965943248698E-4	0.0	0.01572980557041786	0.0
32-33	2.6243679783804564E-4	6.888965943248698E-4	0.0	0.02179865652042267	0.0
34-35	2.9524139756780134E-4	7.709080936492592E-4	0.0	0.027457449973805528	0.0
36-37	2.9524139756780134E-4	8.529195929736484E-4	0.0	0.03474007111381129	0.0
38-39	2.9524139756780134E-4	8.857241927034041E-4	0.0	0.04997780768828282	0.0
40-41	2.9524139756780134E-4	0.0010825517910819384	0.0	0.06864362493451381	0.0
42-43	2.9524139756780134E-4	0.0011645632904063276	0.0	0.08463586730276973	1.6402299864877852E-5
44-45	2.9524139756780134E-4	0.001344988588919984	0.0	0.10112017866697197	3.2804599729755704E-5
46-47	2.9524139756780134E-4	0.0014434023881092512	0.0	0.1247230881725312	3.2804599729755704E-5
48-49	2.9524139756780134E-4	0.0014434023881092512	0.0	0.14601327339714265	3.2804599729755704E-5
50-51	3.280459972975571E-4	0.0017058391859472968	0.0	0.15988961908282934	3.2804599729755704E-5
52-53	3.280459972975571E-4	0.0017550460855419304	0.0	0.18734706905663484	6.560919945951141E-5
54-55	3.280459972975571E-4	0.0017714483854068082	0.0	0.21746169160855058	1.1481609905414497E-4
56-57	3.280459972975571E-4	0.0017714483854068082	0.0	0.2716712926619719	1.3121839891902282E-4
58-59	3.280459972975571E-4	0.0017714483854068082	0.0	0.3198448473651182	1.3121839891902282E-4
60-61	3.7725289689219063E-4	0.0017714483854068082	0.0	0.3401016876982423	1.3121839891902282E-4
62-63	4.592643962165799E-4	0.0017714483854068082	0.0	0.3609818154262318	1.3121839891902282E-4
64-65	4.592643962165799E-4	0.002099494382704365	0.0	0.369412597556779	1.3121839891902282E-4
66-67	4.592643962165799E-4	0.002132298982434121	0.0	0.3752026094090809	1.3121839891902282E-4
68-69	4.592643962165799E-4	0.0021487012822989987	0.0	0.3820095638530052	1.3121839891902282E-4
70-71	4.592643962165799E-4	0.002165103582163877	0.0	0.3870778745112525	1.3121839891902282E-4
72-73	4.592643962165799E-4	0.002165103582163877	0.0	0.3909816218790934	1.6402299864877855E-4
74-75	4.592643962165799E-4	0.0021815058820287545	0.0	0.3941636680528797	1.6402299864877855E-4
76-77	4.592643962165799E-4	0.002263517381353144	0.0	0.39747693262558503	1.6402299864877855E-4
78-79	4.592643962165799E-4	0.0022963219810828994	0.0	0.39949441550896503	1.6402299864877855E-4
80-81	4.592643962165799E-4	0.0022963219810828994	0.0	0.4003965420015333	1.6402299864877855E-4
82-83	4.592643962165799E-4	0.0022963219810828994	0.0	0.40129866849410156	1.9682759837853425E-4
84-85	4.920689959463356E-4	0.0022963219810828994	0.0	0.402315611085724	1.9682759837853425E-4
86-87	4.920689959463356E-4	0.0022963219810828994	0.0	0.40270926628248105	2.132298982434121E-4
88	4.920689959463356E-4	0.0023291265808126553	0.0	0.4027420708822108	2.2963219810828996E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5430	0.0	51.77116	1
GTATCAA	10600	0.0	39.140076	1
CTTATAC	3725	0.0	31.932917	1
TCAACGC	13540	0.0	30.246243	4
ATCAACG	13585	0.0	30.176352	3
TATCAAC	13660	0.0	30.07093	2
CAACGCA	13745	0.0	29.765192	5
AACGCAG	13795	0.0	29.687141	6
TATACAC	4875	0.0	27.270615	3
CGCAGAG	15080	0.0	27.158772	8
ACGCAGA	15635	0.0	26.193422	7
GCAGAGT	16070	0.0	24.590054	9
GAGTACT	10000	0.0	23.817993	12-13
TTATACA	5260	0.0	23.318335	2
GTACATG	9840	0.0	22.58737	1
AGAGTAC	14230	0.0	22.201887	10-11
TACATGG	9920	0.0	22.073286	2
CAGAGTA	15165	0.0	21.579382	10-11
AGTACTT	10560	0.0	21.250088	12-13
TACACAT	6260	0.0	21.03985	5
>>END_MODULE